欧前胡素逆转A2780/Taxol多药耐药作用及其机制研究
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摘要
目的:探讨欧前胡素对人卵巢癌耐紫杉醇细胞株A2780/Taxol耐药性的逆转作用及其可能的作用机制。方法:采用四甲基偶氮唑盐(MTT)比色法检测欧前胡素对人卵巢癌细胞A2780和人卵巢癌耐紫杉醇细胞株A2780/Taxol增殖的影响;观察欧前胡素作用A2780/Taxol细胞24h之后对紫杉醇耐药性的逆转效果;采用高效液相-荧光检测法和Western blot分别对其机制进行研究。结果:A2780/Taxol对紫杉醇耐药倍数为4.34(1.5 Objective: To study the reverse effect and mechanism of imperatorin on the drug resistance of A2780/Taxol cells. Methods: MTT was used to study its effect on proliferation of A2780 and A2780/Taxol cell lines. Drug resistance of A2780/Taxol cell lines against paclitaxel was observed after treated by imperatorin for 24 hours. HPLC-FLD and western blot were used to study its mechanism. Results: The results showed that the multidrug resistance of A2780/Taxol against paclitaxel was 4.34 times(1.5
引文
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[21]葛倩,杨振弢,王邈,等.西黄丸逆转大肠癌细胞株多药耐药的体外研究 .中华中医药学刊,2018,36(1)∶176～180.
[2]汤涛,董伟,张婧,等.中药单体成分逆转肿瘤多药耐药的研究进展 .中草药,2017,48(04)∶792～797+846.
[3]李建华,秦风绮,杨佩满.蝙蝠葛碱逆转K562/ADM细胞多耐药性的研究.大连医科大学学报,2002,24∶94～96
[4]汤涛,廖正根,董伟,等.中药四气属性与逆转肿瘤多药耐药功能关联规律探讨 .中国中药杂志,2017,42(4)∶795～799.
[5]Ewelina Koziol,Krystyna Skalicka-Wozniak.Imperatorin-pharmacological meaning and analytical clues:profound investigation .Phytochemistry Reviews,2016,15(4)∶627～649.
[6]Kanuengnit Choochuay,Preedakorn Chunhacha.Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells.Journal of Natural Medicines,2013,67(3)∶599～606.
[7]Luo K,Sun J,Chan J Y W,et al.Anticancer Effects of Imperatorin Isolated from Angelica dahurica:Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways .Chemotherapy,2011,57(6)∶449～459.
[8]黄志平,邵立龙,阮阳平.欧前胡素对顺铂的骨肉瘤细胞抗肿瘤作用的增效作用 .中国现代应用药学,2015,10∶1193～1197.
[9]郑颖,姜凯.欧前胡素增强多柔比星对HeLa细胞的抗肿瘤效应 .中国病理生理杂志,2015,9∶1578～1583.
[10]Dorota Badziul,Joanna Jakubowicz-Gil,Roman Paduch,et al.Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells..Molecular and Cellular Biochemistry,2014,392(1-2)∶213～227.
[11]Joanna Jakubowicz-Gil,Roman Paduch.Cell death in HeLa cells upon imperatorin and cisplatin treatment Cell death in HeLa cells upon imperatorin and cisplatin treatment .Folia Histochemica Et Cytochemica,2012,50(3)∶381～391.
[12]Dorota Badziula,Joanna Jakubowicz-Gila,et al.The effect of quercetin and imperatorin on programmed cell death induction in T98G cells in vitro..Pharmacological Reports,2014,66(2)∶292～300.
[13]关延彬,田雨冬,周树瑶,等.Pluronic/SWCNTs纳米复合物的制备及对罗丹明123在Caco-2细胞摄取影响的初步研究 .中国新药杂志,2016,25(4)∶453～458.
[14]黄蓓蓓,任非,李国锋,等.罗丹明123经大鼠空肠黏膜的体外Ussing chamber渗透研究 .中国医院药学杂志,2008(17)∶1440～1442.
[15] 陈冲,焦宁,靖景艳,等.台盼蓝拒染法、MTT法、CCK-8法在研究As2O3细胞毒性作用中的意义 .中国医药导报,2013,10(12)∶24～26.
[16]彭有梅,王宁,王亚峰,等.盐酸千金藤碱逆转K562/ADR细胞多药耐药性及其机制.药学学报,2012,47(5)∶594～599.
[17]Snow K,Judd W.characterisation of adriamycin and amscacrine-resistant human leukaenic T cell lines.British Journal of Cancer,1991,63(11)∶17～28.
[18]张亚南,何冰玉,苏荣健,等.雷公藤红素通过逆转EMT抑制SKOV-3细胞的侵袭转移 .中药药理与临床,2018,34(4)∶36～40.
[19]郭明伟,侯凌波,谢建兴.穿心莲内酯对前列腺癌PC-3细胞的凋亡作用及机制研究 .中药药理与临床,2015,31(6)∶18～20.
[20]时文波,甘为,黄雪莲.白桦脂酸诱导乳腺癌细胞MCF-7凋亡的机制研究 .中药药理与临床,2015,31(6)∶39～43.
[21]葛倩,杨振弢,王邈,等.西黄丸逆转大肠癌细胞株多药耐药的体外研究 .中华中医药学刊,2018,36(1)∶176～180.