龙须藤化学成分及其抗类风湿性关节炎活性研究
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摘要
目的:研究龙须藤的化学成分及其对滑膜细胞的抑制作用。方法:运用硅胶柱层析、Sephadex LH-20凝胶柱色谱等方法,对龙须藤乙酸乙酯部位化学成分进行分离纯化,利用现代波谱学技术鉴定化合物结构,采用MTT法对分离得到的单体化合物进行体外抑制滑膜细胞增殖的活性筛选。结果:从龙须藤乙酸乙酯部位分离得到9个化合物,分别鉴定为:豆甾醇-4-烯-3-酮(1)、β-谷甾醇棕榈酸酯(2)、1-(2,4-二羟基苯基)-3-(3,4-二羟基苯基)-3-羟丙基-1-酮(3)、7-羟基香豆素(4)、没食子酸(5)、松柏醛(6)、1-(3,4-二甲氧基苯基)-1-丙醇(7)、丁香酸(8)、没食子酸乙酯(9)。结论:其中,化合物1～4、6、7、9为首次从龙须藤中分离得到,化合物1、3、6、7、9为首次从该属植物中分离得到。MTT法结果显示化合物1～9均对滑膜细胞具有一定的抑制作用,其中化合物5抑制作用最强。
Objective:To study the chemical constituents of the Bauhinia championii rattan,and to explore the effect on proliferation of synovial cells.Methods:The compounds in the ethyl acetate extract of Bauhinia championii rattan were isolated by means of silica gel column chromatography,Sephadex LH-20.Their structures were identified on the basis of modern spectral data analysis.The effect of the compounds on the proliferation of synovial cells were studied by MTT assay.Results:Nine compounds were isolated from the ethyl acetate extract of Bauhinia championii rattan,and were identified as stigmast-4-en-3-one(1),β-sitosterol palmitate(2),1-(2,4-dihydroxyphenyl)-3-(3,4-dihydroxyphenyl)-3-hydroxypropan-1-one(3),umbelliferone(4),gallic acid(5),ferulyl aldehyde(6),1-(3,4-dimethoxyphenyl)-1-propanol(7),syringic acid(8),ethyl gallate(9).Conclusion:Compounds 1～4,6,7,9 are isolated from this plant for the first time,compounds 1,3,6,7,9 are obtained from this genus for the first time.MTT results show that compounds 1～9 had a certain effect on the proliferation of synovial cells,especially compound 5.
Objective:To study the chemical constituents of the Bauhinia championii rattan,and to explore the effect on proliferation of synovial cells.Methods:The compounds in the ethyl acetate extract of Bauhinia championii rattan were isolated by means of silica gel column chromatography,Sephadex LH-20.Their structures were identified on the basis of modern spectral data analysis.The effect of the compounds on the proliferation of synovial cells were studied by MTT assay.Results:Nine compounds were isolated from the ethyl acetate extract of Bauhinia championii rattan,and were identified as stigmast-4-en-3-one(1),β-sitosterol palmitate(2),1-(2,4-dihydroxyphenyl)-3-(3,4-dihydroxyphenyl)-3-hydroxypropan-1-one(3),umbelliferone(4),gallic acid(5),ferulyl aldehyde(6),1-(3,4-dimethoxyphenyl)-1-propanol(7),syringic acid(8),ethyl gallate(9).Conclusion:Compounds 1～4,6,7,9 are isolated from this plant for the first time,compounds 1,3,6,7,9 are obtained from this genus for the first time.MTT results show that compounds 1～9 had a certain effect on the proliferation of synovial cells,especially compound 5.
引文
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[2] 郑海音,徐伟,李煌,等.龙须藤抗类风湿关节炎有效部位的研究[J].浙江中医药大学学报,2013,37(3):321-325.
[3] Costa EV,Sampaio MFC,Salvador MJ,et al.Chemical constituents from the stem bark of Annona pickelii(Annonaceae)[J].Química Nova,2015,38(6):769-776.
[4] Yun XJ,Shu HM,Chen GY,et al.Chemical constituents from barks of Lannea coromandelica[J].Chinese Herbal Medicines,2014,6(1):65-69.
[5] Kim KH,Moon E,Choi SU,et al.Polyphenols from the bark of Rhus verniciflua and their biological evaluation on antitumor and anti-inflammatory activities[J].Phytochemistry,2013,92(4):113-121.
[6] 石磊岭,马国需,杨峻山,等.天山假狼毒的化学成分研究[J].中草药,2016,47(2):223-226.
[7] Li B,Gao J Y,Gong L M,et al.Chemical constituents from Rhus chinensis fruit dregs[J].Journal of Chinese Medicinal materials,2015,38(6):1209-1211.
[8] Haruna M,Koube T,Ito K,et al.Balanophonin,a new neo-lignan from Balanophora japonica Makino[J].Chemical and Pharmaceutical Bulletin,1982,30(4):1525-1527.
[9] Zacchino SA,López SN,Pezzenati GD,et al.In vitro evaluation of antifungal properties of phenylpropanoids and related compounds acting against dermatophytes[J].Journal of Natural Products,1999,62(10):1353-1357.
[10] 王绍军,吴闯,赵赶.帘子藤藤茎化学成分研究[J].中药材,2016,39(2):326-328.
[11] 黄建猷,卢文杰,谭晓,等.广西特色药材退黄藤化学成分的分离与鉴定[J].中国实验方剂学杂志,2016,22(16):49-52.