片仔癀对四氯化碳诱导肝纤维化大鼠肝功能及核因子-κB蛋白表达的影响
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摘要
目的观察片仔癀对CCl_4诱导的肝纤维化大鼠肝功能和核因子-κB(NF-κB)蛋白表达的影响,探讨其作用机制。方法将24只大鼠随机分为正常对照组、模型组、片仔癀组、秋水仙碱组,每组6只,50%CCl_4灌胃8周制备肝纤维化模型。末次给药1 h后,血清测定ALT、AST、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白和透明质酸含量,取肝脏计算肝指数,HE染色和VG染色观察肝组织病理形态,Western blot检测NF-κB蛋白表达。结果与正常对照组比较,模型组大鼠肝指数及ALT、AST、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白、透明质酸含量和NF-κB蛋白表达明显升高(P<0.01);与模型组比较,各给药组大鼠肝指数及ALT、AST、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白、透明质酸含量和NF-κB蛋白表达均明显降低(P<0.01)。结论片仔癀对CCl_4所致肝纤维化大鼠具有保护作用,其机制可能与抑制NF-κB的表达有关。
Objective To investigate the protective effects of Pien Tze Huang on liver function and NF-κB protein expression in rats with hepatic fibrosis induced by CCl_4; To discuss its mechanism of action. Methods Totally 24 rats were randomly divided into four groups: normal group, model group, Pien Tze Huang group and colchicine group, with 6 rats in each group. The liver fibrosis model was prepared by intragastric administration of 50% CCl_4 for 8 weeks. After 1 hour of the last administration, serum was used to determine the contents of ALT, AST, type Ⅲprocollagen, type collagen, laminin and hyaluronic acid. Ⅳ Liver was taken to calculate the liver index. HE and VG staining were performed to observe the pathological damage. The expression level of NF-κB protein was determined by Western blot. Results Compared with the normal group, the levels of liver index, ALT, AST, type procollagen, Ⅲtype Ⅳ collagen, laminin, hyaluronic acid and NF-κB protein in the model group significantly increased(P<0.01). Compared with the model group, the liver index, ALT, AST, type Ⅲprocollagen, type Ⅳcollagen, laminin, hyaluronic acid and NF-κB protein significantly decreased in each administration group(P<0.01, P<0.05). Conclusion Pien Tze Huang has a significant protective effect on hepatic fibrosis induced by CCl_4, which may be related to the inhibition of NF-κB expression.
Objective To investigate the protective effects of Pien Tze Huang on liver function and NF-κB protein expression in rats with hepatic fibrosis induced by CCl_4; To discuss its mechanism of action. Methods Totally 24 rats were randomly divided into four groups: normal group, model group, Pien Tze Huang group and colchicine group, with 6 rats in each group. The liver fibrosis model was prepared by intragastric administration of 50% CCl_4 for 8 weeks. After 1 hour of the last administration, serum was used to determine the contents of ALT, AST, type Ⅲprocollagen, type collagen, laminin and hyaluronic acid. Ⅳ Liver was taken to calculate the liver index. HE and VG staining were performed to observe the pathological damage. The expression level of NF-κB protein was determined by Western blot. Results Compared with the normal group, the levels of liver index, ALT, AST, type procollagen, Ⅲtype Ⅳ collagen, laminin, hyaluronic acid and NF-κB protein in the model group significantly increased(P<0.01). Compared with the model group, the liver index, ALT, AST, type Ⅲprocollagen, type Ⅳcollagen, laminin, hyaluronic acid and NF-κB protein significantly decreased in each administration group(P<0.01, P<0.05). Conclusion Pien Tze Huang has a significant protective effect on hepatic fibrosis induced by CCl_4, which may be related to the inhibition of NF-κB expression.
引文
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[3]YANNG J J,TAO H,LI J.Hedgehog s ignaling pathwaa y as key player r in lii ver fibrosis:n new insights and d perspectives[[J].Expert Opin n Ther Targets,2014,118(9):1011-1021.
[4]王晨晨晓,罗伟生,郭潇,等.络化纤丸联合核苷(酸)类类药物治疗慢性乙乙型肝炎肝纤维化的系系统评价[J].中国国实验方剂学杂志志,2015,21(7):203-2209.
[5]CHEENG Q,LI N,CHHEN M,et al.FFuzheng Huayu ii nhibits carbon n tetraa chloride-induc ced liver fibros sis in mice throo ugh activating g hepatt ic NK cells[J].J Ethnophama acol,2013,145(1)):175-181.
[6]叶静静,刘清华,王璐璐璐.抗肝纤维化药药物治疗的研究进展[J].山东医医药,20014,54(42):1011-103.
[7]曾璐璐,周本杰.肝纤维化发病机制和治疗方法的研究进展[J].广东医医药,20014,35(24):39118-3921.
[8]国家家药典委员会.中中华人民共和国药药典:一部[M].北北京:中国医药科科技出版版社,2015:670.
[9]李倩倩.白藜芦醇介导导NF-κB转录及PI3K磷酸化抑制制肝星状细胞活化化的分子子机制研究[D].延延边:延边大学,2013.
[10]吴吴楠.NF-κB在胰胰腺星状细胞活化化中的作用及黄芩芩苷对其干预机制制研究[D].咸阳:陕西中中医药大学,2017.
[11]洪洪绯,赵锦燕,张泽泽修,等.复方片仔仔癀肝保片对大鼠鼠四氯化碳诱导的的慢性肝肝损伤的治疗作用用[J].福建中医药药,2014,45(5):553-54,60.
[12]率率红莉,方步武,邢邢伟,等.蒿鳖养阴阴软坚方对四氯化化碳复合因素所致致肝纤维维化的预防作用[[J].中国实验方剂剂学杂志,2013,199(16):197-201.
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