紫锥菊单咖啡酰酒石酸和菊苣酸提取工艺优化
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摘要
采用正交试验设计,选取乙醇体积浓度百分比、提取温度、提取时间、抗氧化剂用量等因素,优化紫锥菊Echinacea purpurea单咖啡酰酒石酸和菊苣酸的加热回流提取工艺,并考察加入抗氧化剂对提取效果的影响。结果表明,优化的提取条件是以25%乙醇,在80℃回流提取90 min。抗氧化剂用量对提取效果影响不显著。优化后的加热回流提取条件对紫锥菊单咖啡酰酒石酸和菊苣酸的提取均适用,提取中无需加入抗氧化剂。
To optimize the reflux extraction process of caftaric acid and cichoric acid in Echinacea purpurea, ethanol concentration, extraction temperature, extraction time, and antioxidant amount were optimized by orthogonal experiments. And the addition of antioxidants on the extraction result was evaluated by comparative tests. The results showed that optimized conditions were extracted by 25% ethanol aqueous solution at 80 ℃ for 90 min. The addition of antioxidant had no significant effect on the extraction result. The optimized conditions of reflux extraction were applicable to the extraction of caftaric acid and cichoric acid in E. purpurea. It was not necessary to add antioxidant in extraction.
To optimize the reflux extraction process of caftaric acid and cichoric acid in Echinacea purpurea, ethanol concentration, extraction temperature, extraction time, and antioxidant amount were optimized by orthogonal experiments. And the addition of antioxidants on the extraction result was evaluated by comparative tests. The results showed that optimized conditions were extracted by 25% ethanol aqueous solution at 80 ℃ for 90 min. The addition of antioxidant had no significant effect on the extraction result. The optimized conditions of reflux extraction were applicable to the extraction of caftaric acid and cichoric acid in E. purpurea. It was not necessary to add antioxidant in extraction.
引文
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[17]陈育如,柯小红,闫莽,钱媛媛.翅果菊为原料同时提取菊苣酸、单咖啡酰酒石酸、3,5-二咖啡酰奎尼酸和绿原酸的方法,CN103524347A[P].2014-01-22.
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[3]Fonseca F N,Papanicolaou G,Lin H,Lau C B,Kennelly E J,Cassileth B R.Echinacea purpurea(L.)Moench modulates human T-cell cytokine response[J].International Immunopharmacology,2014,19(1):94—102.
[4]Goel V,Lovlin R,Chang C,Slama J V,Barton R,Gahler R,Bauer R,Goonewardene L,Basu T K.A proprietary extract from the Echinacea plant(Echinacea purpurea)enhances systemic immune response during a common cold[J].Phytotherapy Research,2005,19(8):689—94.
[5]陈荣,杨跃生,吴鸿.广州地区紫锥菊引种与露地栽培试验初报[J].广东农业科学,2011,38(10):20—22.
[6]郑文,李宗周.齐鲁动保新产品获国家一类新兽药证书[J].中国禽业导刊,2012(12):56—56.
[7]Stuart D L,Wills R B H.Factors affecting the extraction of alkylamides and cichoric acid during ethanolic processing of Echinacea purpurea(L.)Moench[J].Animal Production Science,2000,40(6):873—877.
[8]吴启林,袁其朋,陈养文.紫锥菊中菊苣酸提取纯化工艺研究[J].中草药,2004,35(9):995—997.
[9]张雷,邹任贤,邓韵,张干元,欧杰迪.紫锥菊有效部位提取工艺的研究[J].中成药,2008,30(4):603—605.
[10]张广求,王亚洲,袁永华,王伯初.紫锥菊中有效成分的分离提取工艺研究[J].云南民族大学学报(自然科学版),2010,19(2):150—153.
[11]郭喜宝,杨红玉,刘利国.紫锥菊有效成分提取工艺研究[J].西北药学杂志,2011,26(4):245—247.
[12]辛杰,韩琳娜,周凤琴.正交试验法优选紫锥菊中菊苣酸提取工艺[J].山东中医药大学学报,2012(6):539—541.
[13]马晓玲,魏鸿雁,冯薇源,费飞.正交设计优化紫锥菊提取工艺研究[J].新疆中医药,2014,32(3):52—54.
[14]British Pharmacopoeia Commission.British Pharmacopoeia 2013[S].The Stationery Office,London,2013,3681.
[15]郭建伟.紫锥菊中菊苣酸和单咖啡酰酒石酸纯化工艺及菊苣酸性质研究[D].长沙:湖南师范大学硕士论文,2009.
[16]谢春燕,徐新军,谢鸷生,杨梅,黄洁云,林妙卿,杨得坡.快速制备液相色谱分离紫锥菊中咖啡酰基酒石酸、菊苣酸和松果菊苷[J].中药新药与临床药理,2012,23(1):90—94.
[17]陈育如,柯小红,闫莽,钱媛媛.翅果菊为原料同时提取菊苣酸、单咖啡酰酒石酸、3,5-二咖啡酰奎尼酸和绿原酸的方法,CN103524347A[P].2014-01-22.