福建省浙江红花油茶遗传多样性的ISSR分析
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摘要
【目的】为福建省浙江红花油茶的良种选育与充分开发利用提供参考。【方法】以福建省13个居群198份浙江红花油茶为实验材料,对其进行遗传多样性的ISSR分子标记分析。【结果】17个ISSR引物扩增出167条位点,其中多态性位点142条,平均多态位点百分率为85.03%。13个居群浙江红花油茶的遗传距离的变化范围为0.145 9~0.509 4。通过遗传距离进行聚类分析,可将13个天然居群的浙江红花油茶分为4个大类。【结论】福建省不同地区的浙江红花油茶遗传多样性丰富,居群间亲缘关系差异明显。
【Objective】The objective of this study was to provide a reference for fully exploiting and utilizing the germplasm resources of Camellia chekiangoleosa Hu in Fujian province.【Method】The genetic diversity of 13 populations of C. chekiangoleosa in Fujian Province was analyzed by ISSR molecular marker technique.【Results】The results showed that 17 primers were obtained and 167 loci were amplified; in addition,polymorphic loci was 142 and the average percentage of polymorphic loci was 85.03%.The genetic distance of 13 populations of C. chekiangoleosa in Fujian province ranged from 0.745 1 to 0.940 2. The 13 populations of C. chekiangoleosa in Fujian province were divided into four groups by cluster analysis based on genetic distance.【Conclusion】The genetic diversity of C. chekiangoleosa in different areas of Fujian Province was rich and the genetic relationship among populations was significant.
【Objective】The objective of this study was to provide a reference for fully exploiting and utilizing the germplasm resources of Camellia chekiangoleosa Hu in Fujian province.【Method】The genetic diversity of 13 populations of C. chekiangoleosa in Fujian Province was analyzed by ISSR molecular marker technique.【Results】The results showed that 17 primers were obtained and 167 loci were amplified; in addition,polymorphic loci was 142 and the average percentage of polymorphic loci was 85.03%.The genetic distance of 13 populations of C. chekiangoleosa in Fujian province ranged from 0.745 1 to 0.940 2. The 13 populations of C. chekiangoleosa in Fujian province were divided into four groups by cluster analysis based on genetic distance.【Conclusion】The genetic diversity of C. chekiangoleosa in different areas of Fujian Province was rich and the genetic relationship among populations was significant.
引文
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[14]杜明凤,丁贵杰.不同种源马尾松ISSR遗传结构及影响因素分析[J].广西植物,2016,36(9):1068-1075.
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[17]谢云,李纪元,范正琪,等.浙江红山茶总DNA提取及ISSR-PCR引物筛选[J].安徽农业科学,2011,39(25):15210-15212.
[18]刘扬,彭赟,贺瑞,等.基于ISSR标记的海南油茶种质资源遗传多样性分析[J].分子植物育种,2016(2):517-523.
[19]彭继庆,曹福祥,范海燕.博白大果油茶遗传多样性的ISSR研究[J].中南林业科技大学学报,2013,33(7):62-66.
[20]刘子雷,姚小华,杨水平,等.浙江红花油茶果实经济性状变异的研究[J].西南大学学报(自然科学版),2007,29(4):83-88.
[2]祈承经,汤庚国.树木学(第二版)[M].中国林业出版社,2010:304-305.
[3]杨军,邱日红,赵文进,等.浙江红花油茶种质资源及开发利用[J].现代园艺,2012(9):12-13.
[4]何方,胡芳名.经济林栽培学(第二版)[M].中国林业出版社,2010:279-280.
[5]刘曲,高焕章,姚小华,等.低海拔地区浙江红花油茶无性系树体性状变异研究[J].湖北农业科学,2015,54(5):1105-1108.
[6]蹇黎.分子标记在药用兰科植物研究中的应用[J].种子,2015,34(2):51-53.
[7]靳晓丽,田新会,杜文华.基于ISSR分子标记的鹰嘴豆遗传多样性分析[J].中国草地学报,2015,37(3):19-25.
[8]赵谦,杜虹,庄东红.ISSR分子标记及其在植物研究中的应用[J].2007,5(f11):123-129.
[9]HAMZA H,BENABDERRAHIM M A,ELBEKKAY M,et al.Investigation of genetic variation in Tunisian date palm(Phoenix dactylifera L).cultivars using ISSR marker systems and their relation with fruit characteristic[sJ].Turkish Journal of Biology,2012,18(3):196-198.
[10]QI S S,FAN Y,GONG Z N,et al.Genetic diversity of Shiraia bambusicola from East China assessed using ISSR makers[J].Biochemical Systematics and Ecology,2015(59):239-245.
[11]周兰英,王湘南,陈永忠,等.油茶种质遗传多样性的分子标记技术与EST文库构建[J].生命科学研究,2012,16(6):557-565.
[12]董斌,李荣喜,黄永芳,等.分子标记在油茶研究中的应用[J].生物技术通报2015,31(6):74-80.
[13]SUN L,ZHENG Y,Yuan,et al.Genetic diversity of Atractylodes macrocephala by ISSR[J].China journal of Chinese materia edica,2013,37(22):3381-3385.
[14]杜明凤,丁贵杰.不同种源马尾松ISSR遗传结构及影响因素分析[J].广西植物,2016,36(9):1068-1075.
[15]周兰英.油茶种质资源多样性的ISSR分析[D].湖南师范大学,2014,
[16]谢荟.黎蒴与广宁红花油茶种质资源遗传多样性研究[D].华南农业大学,2016.
[17]谢云,李纪元,范正琪,等.浙江红山茶总DNA提取及ISSR-PCR引物筛选[J].安徽农业科学,2011,39(25):15210-15212.
[18]刘扬,彭赟,贺瑞,等.基于ISSR标记的海南油茶种质资源遗传多样性分析[J].分子植物育种,2016(2):517-523.
[19]彭继庆,曹福祥,范海燕.博白大果油茶遗传多样性的ISSR研究[J].中南林业科技大学学报,2013,33(7):62-66.
[20]刘子雷,姚小华,杨水平,等.浙江红花油茶果实经济性状变异的研究[J].西南大学学报(自然科学版),2007,29(4):83-88.