白芷叶总香豆素的大孔树脂纯化工艺
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摘要
采用单因素与正交试验结合的方法对白芷叶总香豆素的大孔树脂纯化工艺进行研究,发现其优选工艺为:大孔树脂型号为D-101、上柱液的质量浓度为0.2 g/mL、上柱体积为3 BV,上柱液pH为4,上柱液流速为2 BV/h,洗脱用乙醇体积分数为90%,洗脱速度为2 BV/h,洗脱体积为6 BV.采用该纯化工艺,可使白芷叶总香豆素的纯度达到58.3%.
The method of combining single factor with orthogonal test was used to study the purification process of macroporous resin from total coumarin of Angelica Dahurica leaves. The optimized process is as follows: the macroporous resin model is D-101,the concentration of the sample solution is 0.2 g/mL, the volume of the supernatant is 3 BV, the pH of the sample is 4, the loading speed is 2 BV/h, the ethanol volume fraction is 90%, the elution rate is 2 BV/h and the elution volume is 6 BV. Using the optimized process, total coumarin purity of Angelica Dahurica leaves is up to 58.3%.
The method of combining single factor with orthogonal test was used to study the purification process of macroporous resin from total coumarin of Angelica Dahurica leaves. The optimized process is as follows: the macroporous resin model is D-101,the concentration of the sample solution is 0.2 g/mL, the volume of the supernatant is 3 BV, the pH of the sample is 4, the loading speed is 2 BV/h, the ethanol volume fraction is 90%, the elution rate is 2 BV/h and the elution volume is 6 BV. Using the optimized process, total coumarin purity of Angelica Dahurica leaves is up to 58.3%.
引文
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[7]倪红霞,王春梅.白芷总香豆素联合白芷挥发油对大鼠偏头痛的预防作用及其机制[J].吉林大学学报(医学版),2018,44(3):487-492.
[8]于定荣,麻印莲,顾雪竹,等.不同提取方法对白芷中香豆素成分欧前胡素、异欧前胡素的影响及镇痛作用的比较研究[J].时珍国医国药,2016,27(10):2405-2407.
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[10]杨正明,滕云,韩亚涛,等.响应面法优化藏药毛莲蒿总香豆素的超声辅助提取工艺[J].西南师范大学学报(自然科学版),2016,41(6):74-80.
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[12]蒋林,米阿娜,罗宇东,等.星点设计-响应面法优选龙钻通痹方总生物碱大孔树脂纯化工艺[J].中草药, 2018, 49(2):337-345.
[13]王蓉蓉,杨素芳,王英豪,等.羌活-独活合提液中总香豆素的大孔吸附树脂纯化工艺优选[J].中国实验方剂学杂志,2014,20(15):25-27.
[14]张华潭,郑文丽,魏艳婷,等.大孔树脂纯化黄蜀葵花总黄酮的工艺优选[J].中国实验方剂学杂志,2015,21(1):28-31.
[15] LIU C X, LIU R C, ZHANG P, et al. Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography[J]. Journal of Separation Science, 2015,38(23):4141–4145.
[16]王瑞海,柏冬,刘寨华,等.秦皮总香豆素大孔树脂纯化工艺研究[J].中草药,2015,46(22):3344-3351.
[17]张春梅,陈朝银,赵声兰,等.大孔吸附树脂纯化核桃内种皮总多酚的工艺优选[J].中国实验方剂学杂志,2014,20(17):27-30.
[18]阿依姑丽·艾合麦提,李珊,英提扎尔·艾合买提江,等.野山杏果肉总有机酸的分离纯化及其清除自由基能力的研究[J].食品科技,2017,42(5):219-224.
[19]金晨,罗晶,张武岗,等.东北铁线莲中抗肿瘤活性成分的大孔树脂纯化工艺优选[J].中国实验方剂学杂志,2016,22(15):14-17.
[20]王凯顺,袁慕华,刘璇,等.大孔树脂富集多舌飞蓬总咖啡酸酯的工艺[J].精细化工,2017,34(2):186-190.
[21]钟方丽,王晓林,付丽娟,等.大孔树脂法纯化刺玫果总皂苷工艺研究[J].河南工业大学学报(自然科学版),2014,35(1):76-81.
[22]骆党委,叶静,黄雅燕,等.AB-8大孔吸附树脂精制芦柑皮总黄酮及黄酮类化合物的分离[J].食品科学,2014,35(6):30-35.
[23]王瑞海,柏冬,刘寨华,等.秦皮总香豆素大孔树脂纯化工艺研究[J].中草药,2015,46(22):3344-3351.
[2] XU S F, YE Y P, LI X Y, et al. Chemical composition and antioxidant activities of different polysaccharides from the roots of Angelica dahurica[J]. Chemistry&Biodiversity, 2011,8(6):1121-1131.
[3] ZHENG Y M, SHEN J Z, WANG Y, et al. Anti-oxidant and anti-cancer activities of Angelica dahurica extract via induction of apoptosis in colon cancer cells[J]. Phytomedicine,2016,23(11):1267-1274.
[4]王慧君.白芷产新人气浓[J].全国药材信息,2013(23):12-13.
[5]鹿凡存.白芷叶治蜂蜇效果观察[J].山东医药工业,1997,3(12):56-57.
[6] LI B, ZHANG X, WANG J, et al. Simultaneous characterisation of fifty coumarins from the roots of Angelica dahurica by off-line two-dimensional high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry[J]. Phytochemical Analysis,2014,25(3):229-240.
[7]倪红霞,王春梅.白芷总香豆素联合白芷挥发油对大鼠偏头痛的预防作用及其机制[J].吉林大学学报(医学版),2018,44(3):487-492.
[8]于定荣,麻印莲,顾雪竹,等.不同提取方法对白芷中香豆素成分欧前胡素、异欧前胡素的影响及镇痛作用的比较研究[J].时珍国医国药,2016,27(10):2405-2407.
[9]薛天乐.白芷叶总香豆素提取工艺和清除羟自由基活性的研究[J].安徽科技学院学报,2017,31(2):45-50.
[10]杨正明,滕云,韩亚涛,等.响应面法优化藏药毛莲蒿总香豆素的超声辅助提取工艺[J].西南师范大学学报(自然科学版),2016,41(6):74-80.
[11]李浩,赵凤春,袁贤达,等.大血藤药材和饮片中酚类和皂苷类成分的含量[J].中国中药杂志,2015,40(10):1865-1871.
[12]蒋林,米阿娜,罗宇东,等.星点设计-响应面法优选龙钻通痹方总生物碱大孔树脂纯化工艺[J].中草药, 2018, 49(2):337-345.
[13]王蓉蓉,杨素芳,王英豪,等.羌活-独活合提液中总香豆素的大孔吸附树脂纯化工艺优选[J].中国实验方剂学杂志,2014,20(15):25-27.
[14]张华潭,郑文丽,魏艳婷,等.大孔树脂纯化黄蜀葵花总黄酮的工艺优选[J].中国实验方剂学杂志,2015,21(1):28-31.
[15] LIU C X, LIU R C, ZHANG P, et al. Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography[J]. Journal of Separation Science, 2015,38(23):4141–4145.
[16]王瑞海,柏冬,刘寨华,等.秦皮总香豆素大孔树脂纯化工艺研究[J].中草药,2015,46(22):3344-3351.
[17]张春梅,陈朝银,赵声兰,等.大孔吸附树脂纯化核桃内种皮总多酚的工艺优选[J].中国实验方剂学杂志,2014,20(17):27-30.
[18]阿依姑丽·艾合麦提,李珊,英提扎尔·艾合买提江,等.野山杏果肉总有机酸的分离纯化及其清除自由基能力的研究[J].食品科技,2017,42(5):219-224.
[19]金晨,罗晶,张武岗,等.东北铁线莲中抗肿瘤活性成分的大孔树脂纯化工艺优选[J].中国实验方剂学杂志,2016,22(15):14-17.
[20]王凯顺,袁慕华,刘璇,等.大孔树脂富集多舌飞蓬总咖啡酸酯的工艺[J].精细化工,2017,34(2):186-190.
[21]钟方丽,王晓林,付丽娟,等.大孔树脂法纯化刺玫果总皂苷工艺研究[J].河南工业大学学报(自然科学版),2014,35(1):76-81.
[22]骆党委,叶静,黄雅燕,等.AB-8大孔吸附树脂精制芦柑皮总黄酮及黄酮类化合物的分离[J].食品科学,2014,35(6):30-35.
[23]王瑞海,柏冬,刘寨华,等.秦皮总香豆素大孔树脂纯化工艺研究[J].中草药,2015,46(22):3344-3351.