双波长法分析测定汉中莲菜支链淀粉和直链淀粉的含量
|
摘要
用碘为显色剂,采用双波长紫外—可见法检测汉中莲菜中支链淀粉和直链淀粉的含量,并对汉中莲菜进行了评价。根据直链淀粉与碘形成蓝色复合物、支链淀粉与碘形成紫红色复合物的显色特征和双波长法测定结果,确定直链淀粉的测定波长和参比波长分别为511 nm和582 nm,y=0.046 43x+0.011 67(r2=0.998 7);支链淀粉的测定波长和参比波长分别为614 nm和549 nm,y=0.004 6x+0.002 55(r2=0.996 8)。测得汉中不同品种莲菜中的直链淀粉和支链淀粉各不相同,方法的回收率为98.07%~101.3%,稳定性的相对标准偏差(RSD)值分别为1.95%和1.85%,精密度RSD值分别为0.26%和0.11%,结果表明该方法可用于莲菜中淀粉的分析。
Dual-wavelength spectrophotometry was applied to determine the amylose and amylopectin content in lotus root from Hanzhong with iodine as coloring agent. According to the color characteristics of amylose mixed with iodine forming blue mixtures and amylopectin mixed with iodine forming amaranth mixtures, and the determination results of Dual-wavelength spectrophotometry, the determination and reference wavelength of amylose were 511 nm and 582 nm,respectively,Y=0.046 43 x+0.011 67(r2=0.998 7); the determination and reference wavelength of amylopectin were 614 nm and 549 nm, respectively,Y=0.004 6 x+0.002 55(r2=0.996 8). The amylose and amylopectin content was different in different varieties of lotus root from Hanzhong, the recovery rate of both amylose and amylopectin ranged from 98.07% to 101.3%.The RSD of stability was 1.95% and 1.85%, and the RSD of precision is 0.26% and 0.11%,respectively. The results showed the method Could accurately analyze the starch in lotus root.
Dual-wavelength spectrophotometry was applied to determine the amylose and amylopectin content in lotus root from Hanzhong with iodine as coloring agent. According to the color characteristics of amylose mixed with iodine forming blue mixtures and amylopectin mixed with iodine forming amaranth mixtures, and the determination results of Dual-wavelength spectrophotometry, the determination and reference wavelength of amylose were 511 nm and 582 nm,respectively,Y=0.046 43 x+0.011 67(r2=0.998 7); the determination and reference wavelength of amylopectin were 614 nm and 549 nm, respectively,Y=0.004 6 x+0.002 55(r2=0.996 8). The amylose and amylopectin content was different in different varieties of lotus root from Hanzhong, the recovery rate of both amylose and amylopectin ranged from 98.07% to 101.3%.The RSD of stability was 1.95% and 1.85%, and the RSD of precision is 0.26% and 0.11%,respectively. The results showed the method Could accurately analyze the starch in lotus root.
引文
[1]王清章,邱承光,彭光华,等.莲菜粉糊的流变特性实验研究[J].农业工报,2002,18(4):116-119.
[2]廖丽莎,刘宏生,刘兴训,等.淀粉的微观结构与加工过程中相变研究进展[J].高分子学报,2014(6):761-773.
[3]陈旭红.旋光法测定木薯粗淀粉的含量[J].食品工业科技,2000(2):66-67.
[4]郭冬生,彭小兰.蒽酮比色法和酶水解法两种淀粉测定方法的比较研究[J].湖南文理学院学报(自然科学版),2007(3):34-36.
[5]张水华.食品分析[M].北京:中国轻工业出版社,2004.
[6]王宪泽.生物化学实验技术原理和方法[M].北京:中国农业出版社,2002.
[7]GRANT L A,OSTENSON A M,RAYAS-DUARTE P.Determination of Amylose and Amylopectin of Wheat Starch Using High Performance Size-Exclusion Chromatog raphy(HPSEC)[J].Cereal Chemistry,2007,79(6):771-773.
[8]FERTIG C C,PODCZECK F,JEE R D,et al.Feasibility study for the rapid determination of the amylose content in starch by near-infrared spectroscopy[J].European Journal of Pharmaceutical Sciences,2004,21(2-3):155-159.
[9]MOORTHY S N,ANDERSSON L,ELIASSON A C,et al.Determination of Amylose Content in Different Starches Using Modulated Differential Scanning Calorimetry[J].Starch-Starke,2010,58(5):209-214.
[10]王丹,吕冰,周爽,等.高效液相色谱法检测淀粉及含淀粉食品中6种有机酸[J].中国食品添加剂,2014(2):108-113.
[11]加列西·马那甫,景伟文,削合来提·再丁.双波长法测定谷类和豆类作物籽粒中直链和支链淀粉的含量[J].新疆农业科学,2010,47(3):564-568.
[12]蒋卉,胡中泽.双波长法测定籼米中直链淀粉和支链淀粉含量[J].粮食与饲料工业,2013,12(2):22-25.
[13]林美娟,宋江峰,李大婧,等.用双波长分光光度法测定鲜食玉米中直链淀粉和支链淀粉含量[J].江西农业学报,2010,22(12):121-123.
[14]厦门大学化学分析化学教研室.双波长分光光度法原理简介[J].分析化学,1978,6(3):224-231.
[15]钱文文.两类不同质地莲藕的特性研究[D].武汉:华中农业大学,2011.
[16]常云彩.常见食用淀粉特性及掺假检测方法研究[D].郑州:河南工业大学,2015.
[2]廖丽莎,刘宏生,刘兴训,等.淀粉的微观结构与加工过程中相变研究进展[J].高分子学报,2014(6):761-773.
[3]陈旭红.旋光法测定木薯粗淀粉的含量[J].食品工业科技,2000(2):66-67.
[4]郭冬生,彭小兰.蒽酮比色法和酶水解法两种淀粉测定方法的比较研究[J].湖南文理学院学报(自然科学版),2007(3):34-36.
[5]张水华.食品分析[M].北京:中国轻工业出版社,2004.
[6]王宪泽.生物化学实验技术原理和方法[M].北京:中国农业出版社,2002.
[7]GRANT L A,OSTENSON A M,RAYAS-DUARTE P.Determination of Amylose and Amylopectin of Wheat Starch Using High Performance Size-Exclusion Chromatog raphy(HPSEC)[J].Cereal Chemistry,2007,79(6):771-773.
[8]FERTIG C C,PODCZECK F,JEE R D,et al.Feasibility study for the rapid determination of the amylose content in starch by near-infrared spectroscopy[J].European Journal of Pharmaceutical Sciences,2004,21(2-3):155-159.
[9]MOORTHY S N,ANDERSSON L,ELIASSON A C,et al.Determination of Amylose Content in Different Starches Using Modulated Differential Scanning Calorimetry[J].Starch-Starke,2010,58(5):209-214.
[10]王丹,吕冰,周爽,等.高效液相色谱法检测淀粉及含淀粉食品中6种有机酸[J].中国食品添加剂,2014(2):108-113.
[11]加列西·马那甫,景伟文,削合来提·再丁.双波长法测定谷类和豆类作物籽粒中直链和支链淀粉的含量[J].新疆农业科学,2010,47(3):564-568.
[12]蒋卉,胡中泽.双波长法测定籼米中直链淀粉和支链淀粉含量[J].粮食与饲料工业,2013,12(2):22-25.
[13]林美娟,宋江峰,李大婧,等.用双波长分光光度法测定鲜食玉米中直链淀粉和支链淀粉含量[J].江西农业学报,2010,22(12):121-123.
[14]厦门大学化学分析化学教研室.双波长分光光度法原理简介[J].分析化学,1978,6(3):224-231.
[15]钱文文.两类不同质地莲藕的特性研究[D].武汉:华中农业大学,2011.
[16]常云彩.常见食用淀粉特性及掺假检测方法研究[D].郑州:河南工业大学,2015.