高效液相色谱法同时测定湖南不同产地金银花中不同部位绿原酸与木犀草苷含量
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摘要
目的通过高效液相色谱法测定6个产地金银花中不同部位的绿原酸与木犀草苷的含量,考察湖南省金银花的质量情况,为该药材资源的综合开发利用提供科学依据。方法采用高效液相色谱法,色谱柱:Sepax Bio-C18(4.6 mm×250 mm,5μL);流动相:乙腈-0.4%乙酸水溶液;二元梯度洗脱,0～12min(0∶100～5∶95),12～17 min(5∶95～16∶84),17～70 min(16∶84～5∶95);流速1.0mL·min-1,检测波长350 nm,柱温:30℃。结果绿原酸、木犀草苷分别在0.0104～0.4150 mg·mL-1、0.0013～0.0410 mg·mL-1与峰面积线性关系良好,绿原酸的加样回收率在99.5%～109.4%,木犀草苷的加样回收率在93.4%～106.7%,RSD均<3.0%;不同产地金银花中活性成分含量有差异,同一产地金银花不同部位的活性成分也存在明显差异。结论该方法对于金银花中绿原酸和木犀草苷的定量分析简便、快捷、稳定,且重现性良好,对湖南省金银花的各个部位的综合利用具有一定的参考意义。
Objective To determine the content of chlorogenic acid and luteolin in different parts of Lonicera japonica Thunb. from 6 producing areas by HPLC to compare the quality of Lonicera japonica Thunb. in Hunan Province and to provide scientific basis for the comprehensive development and utilization of this medicinal material. Methods Sepax Bio-C18(4.6 mm×250 mm, 5 μm) column was used. The mobile phase was acetonitrile-0.4% acetic acid aqueous solution; binary gradient elution at 0-12 min(0 ∶ 100-5 ∶ 95),12-17 min(5 ∶ 95-16 ∶ 84), and 17-70 min(16 ∶ 84-5 ∶ 95). The flow rate was 1.0 mL·min-1,the detection wavelength was 350 nm, and the column temperature was 30℃. Results Chlorogenic acid and luteolin have good linearity at 0.0104-0.4150 mg·mL-1 and 0.0013-0.0410 mg·mL-1 respectively.The recoveries of chlorogenic acid were between 99.5% and 109.4%, and the recoveries of luteolin were between 93.4% and 106.7%. The RSDs were all less than 3.0%. The content of active ingredients in Lonicera japonica Thunb. from different producing areas was different, and the active components of different parts from the same origin also had significant differences. Conclusion The method is simple, rapid, stable and reproducible for the quantitative analysis of chlorogenic acid and luteolin in Lonicera japonica Thunb. and this study provides reference for the comprehensive utilization of various parts of Lonicera japonica Thunb. in Hunan.
Objective To determine the content of chlorogenic acid and luteolin in different parts of Lonicera japonica Thunb. from 6 producing areas by HPLC to compare the quality of Lonicera japonica Thunb. in Hunan Province and to provide scientific basis for the comprehensive development and utilization of this medicinal material. Methods Sepax Bio-C18(4.6 mm×250 mm, 5 μm) column was used. The mobile phase was acetonitrile-0.4% acetic acid aqueous solution; binary gradient elution at 0-12 min(0 ∶ 100-5 ∶ 95),12-17 min(5 ∶ 95-16 ∶ 84), and 17-70 min(16 ∶ 84-5 ∶ 95). The flow rate was 1.0 mL·min-1,the detection wavelength was 350 nm, and the column temperature was 30℃. Results Chlorogenic acid and luteolin have good linearity at 0.0104-0.4150 mg·mL-1 and 0.0013-0.0410 mg·mL-1 respectively.The recoveries of chlorogenic acid were between 99.5% and 109.4%, and the recoveries of luteolin were between 93.4% and 106.7%. The RSDs were all less than 3.0%. The content of active ingredients in Lonicera japonica Thunb. from different producing areas was different, and the active components of different parts from the same origin also had significant differences. Conclusion The method is simple, rapid, stable and reproducible for the quantitative analysis of chlorogenic acid and luteolin in Lonicera japonica Thunb. and this study provides reference for the comprehensive utilization of various parts of Lonicera japonica Thunb. in Hunan.
引文
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[8]蒋玉林,李春华,蒋枧林. HPLC分析比较金银花花茎叶中绿原酸含量[J].广东化工,2013,40(24):127-129.
[9]黄麟杰,曾爱国. HPLC-DAD法测定“武当二号金银花”、“武当三号金银花”叶中木犀草素的含量[J].中南药学,2017,15(1):107-111.
[10]雷志钧,周日宝,曾嵘,等.灰毡毛忍冬与正品金银花解热作用的比较研究[J].湖南中医药大学学报,2005,25(5):14-15.
[11]蔡嘉洛,易刚强,高昱,等.不同加工方法山银花药材中绿原酸、木犀草苷的含量测定[J].中南药学,2015,13(1):71-74.
[12]刘杨,包华音,张峰.不同产地及品种金银花中绿原酸和木犀草苷含量测定[J].食品研究与开发,2015,36(9):106-109.
[2]杨丽军,王丹丹,张允菲,等.金银花中木犀草苷的研究进展[J].中国医药导报,2017,14(24):58-61.
[3]徐晖.金银花药理作用研究进展[J].湖南中医杂志,2013,29(9):148-150.
[4]熊艳,朱晶晶,王智民,等.金银花与山银花HPLC指纹图谱比较研究[J].湖南中医药大学学报,2010,30(9):90-92.
[5]王玲娜.绿原酸的定量、类似物及衍生物的制备和生物活性研究[D].呼和浩特:内蒙古大学,2017.
[6]杨敏丽,王丽婷.金银花不同品种及不同部位木犀草素含量的比较[J].中华中医药杂志,2007,22(10):666-668.
[7]管仁伟,曲永胜,顾正位,等.木犀草苷的药理作用研究[J].中国野生植物资源,2014,33(1):1-3.
[8]蒋玉林,李春华,蒋枧林. HPLC分析比较金银花花茎叶中绿原酸含量[J].广东化工,2013,40(24):127-129.
[9]黄麟杰,曾爱国. HPLC-DAD法测定“武当二号金银花”、“武当三号金银花”叶中木犀草素的含量[J].中南药学,2017,15(1):107-111.
[10]雷志钧,周日宝,曾嵘,等.灰毡毛忍冬与正品金银花解热作用的比较研究[J].湖南中医药大学学报,2005,25(5):14-15.
[11]蔡嘉洛,易刚强,高昱,等.不同加工方法山银花药材中绿原酸、木犀草苷的含量测定[J].中南药学,2015,13(1):71-74.
[12]刘杨,包华音,张峰.不同产地及品种金银花中绿原酸和木犀草苷含量测定[J].食品研究与开发,2015,36(9):106-109.