不同电极距离下人体小腿组织细胞内外液电阻率的测量
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摘要
目的观察不同电极距离下人体小腿细胞内外液电阻率的均匀性,探究测量系统的可行性,以便应用此系统测量健康信息。方法采用四电极生物阻抗谱法,激励电极将1~100 kHz恒流电流施加到人体小腿两端,测量电极采集电压和相角,电极距离分别为5 cm、10 cm、15 cm。测量10例健康受试者,计算得到细胞内外液的阻抗值和电阻率。结果随两电极间距离的增加,细胞内外液阻抗均极显著增加,阻抗的增加与距离高度相关;细胞内外液电阻率随距离仅有小幅增加,5 cm和15 cm之间有显著性差异,与小腿有效截面积随距离缩小有关。细胞内外液电阻率之比接近1,且不随电极距离的变化而改变。结论健康人体小腿组织的电阻率比较均匀,细胞内外液电阻率基本相等。
Objective To explore the feasibility of tissue fluid resistivity measurement for healthcare management,we evaluated the uniformity of the extracellular( ρe) and intracellular( ρi) fluid resistivity in a lower leg( by measuring at different electrode distances). Methods Ten health volunteers were involved in the test with a four-electrode bioimpedance spectroscopy. The measurement was performed with excitation frequencies from 1 kHz to 100 kHz. Two measuring electrodes were set on one lower leg at the distances of 5 cm,10 cm and 15 cm. Voltage amplitude,phase angle,ρeand ρiwere analyzed. Results The measured impedance increased with the distance of the measuring electrodes,and the resistivity changed little with the distance. No obvious difference between extracellular and intracellular fluid resistivity was observed. In addition,voltage amplitude and phase angle were influenced by the frequencies of excitation signals. Conclusions The resistivity in a lower leg measured with the four-electrode bioimpedance spectroscopy is likely uniform. The resistivities of extracellular fluid and intracellular fluid are similar.
Objective To explore the feasibility of tissue fluid resistivity measurement for healthcare management,we evaluated the uniformity of the extracellular( ρe) and intracellular( ρi) fluid resistivity in a lower leg( by measuring at different electrode distances). Methods Ten health volunteers were involved in the test with a four-electrode bioimpedance spectroscopy. The measurement was performed with excitation frequencies from 1 kHz to 100 kHz. Two measuring electrodes were set on one lower leg at the distances of 5 cm,10 cm and 15 cm. Voltage amplitude,phase angle,ρeand ρiwere analyzed. Results The measured impedance increased with the distance of the measuring electrodes,and the resistivity changed little with the distance. No obvious difference between extracellular and intracellular fluid resistivity was observed. In addition,voltage amplitude and phase angle were influenced by the frequencies of excitation signals. Conclusions The resistivity in a lower leg measured with the four-electrode bioimpedance spectroscopy is likely uniform. The resistivities of extracellular fluid and intracellular fluid are similar.
引文
[1]周黎明,杨玉星,袁世英,等.基于坐标变换迭代拟合法的生物电阻抗模型参数提取方法研究[J].生物医学工程学杂志,2006,23(1):56-59.Zhou LM,Yang YX,Yuan SY,et al.A study of coordinates transform iterative fitting method to extract bio-impedance model parameters[J].Journal of Biomedical Engineering,2006,23(1):56-59.
[2]WD van,Westerterp KR,Wouters L,et al.Validation of bioelectrical-impedance measurements as a method to estimate body-water compartments[J].American Journal of Clinical Nutrition,1994,60(2):159-166.
[3]AC Ahn,Martinsen OG.Electrical characterization of acupuncture points:technical issues and challenges[J].Journal of Alternative&Complementary Medicine,2007,13(8):817-824.
[4]祁朋祥.基于生物电阻抗的人体成分测试与研究[D].合肥:中国科学技术大学,2009.Qi PX.Testing and research of body composition-based on bioelectrical impedance[D].Hefei:University of Science and Technology of China,2009.
[5]Matthie JR,Withers PO,Loan VMD.Development of commercial complexbio-impedance spectroscopic system for determining intracellular and extracellular water volumes[J].In Proceedings of the 8th International Conference on Electrical Bio-impedance,1992,203-205.
[6]庄翠芳,阳波,牛俊泽.多频率激励生物电阻抗测量方法的研究[J].计算机测量与控制,2016,24(5):71-73.Zhuang CF,Yang B,Niu JZ.Research of a bio-electrical impedance measurement based on multi-frequency technology[J].Computer Measurement&Control,2016,24(5):71-73.
[7]Ljungqvist O,Hedenborg G,Jacobson SH.Whole body impedance measurements reflect total body water changes.A study in hemodialysis patients[J].International Journal of Clinical Monitoring and Computing,1990,7(3):163-169.
[8]Souweine G,Lenoir J,Jenin P.Determination of body fluid compartments by impedance measurements:differences in the repartition of water between duchenne muscular dystrophy and some neurological diseases[J].Journal of Neurology,1981,226(3):199-203.
[9]苌飞霸,尹军,颜乐先,等.基于生物电阻抗法的人体成分测量系统的研究与评价[J].中国医学物理学杂志,2014,31(2):4833-4838.Chang FB,Yin J,Yan LX,et al.Research and evaluation on body composition measurement system based on bioelectrical impedance[J].Chinese Journal of Medical Physics,2014,31(2):4833-4838.
[10]云洁,王燕平,张维波,等.应用生物阻抗频谱法对循经体液分布的初步观察[J],中国中医基础医学杂志,2015,21(7):853-856.Yun J,Wang YP,Zhang WB,et al.Preliminary observation on the body fluid distribution along meridian using bioimpedance spectroscopy method[J].Journal of Basic Chinese Medicine,2015,21(7):853-856.
[11]杨威生.低阻经络研究IV对经络生理学功能的推测[J].北京大学学报(自然科学版),2008,44(2):281-288.Yang WS.Investigation of the lower resistance meridian IV.Speculation on the physiological functions of a cupuncture meridians[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2008,44(2):281-288.
[12]张维波,庄逢源,田宇瑛,等.使用凝胶模型法对经络生物物理特性的模拟研究[J].生物医学工程学杂志,2001,18(3):357-361.Zhang WB,Zhuang FY,Tian YY,et al.A simulating study of biophysical features a long meridians on a gel model[J].Journal of Biomedical Engineering,2001,18(3):357-361.
[13]张立剑,张维波,刘雨亭,等.人体电测量技术在经络研究中的运用[J].北京生物医学工程,2000,19(3):156-158.Zhang LJ,Zhang WB,Liu YT,et al.Application of human-body electrical detection techniqu ein meridian study of traditional Chinese medicine[J].Beijing Biomedical Engineering,2000,19(3):156-158.
[14]刘近贞.基于扫描电极的开放式电阻抗成像的研究[D].天津:天津大学,2014.Liu JZ.Study on the open electrical impedance tomography with scanning electrode[D].Tianjin:Tianjin University,2014.
[15]Kanai H,Sakamoto K,Haeno M.Electrical measurement of fluid distribution in human legs:estimation of extra-and intra-cellular fluid volume[J].Journal of Microwave Power,1983,18(3):233-243.
[2]WD van,Westerterp KR,Wouters L,et al.Validation of bioelectrical-impedance measurements as a method to estimate body-water compartments[J].American Journal of Clinical Nutrition,1994,60(2):159-166.
[3]AC Ahn,Martinsen OG.Electrical characterization of acupuncture points:technical issues and challenges[J].Journal of Alternative&Complementary Medicine,2007,13(8):817-824.
[4]祁朋祥.基于生物电阻抗的人体成分测试与研究[D].合肥:中国科学技术大学,2009.Qi PX.Testing and research of body composition-based on bioelectrical impedance[D].Hefei:University of Science and Technology of China,2009.
[5]Matthie JR,Withers PO,Loan VMD.Development of commercial complexbio-impedance spectroscopic system for determining intracellular and extracellular water volumes[J].In Proceedings of the 8th International Conference on Electrical Bio-impedance,1992,203-205.
[6]庄翠芳,阳波,牛俊泽.多频率激励生物电阻抗测量方法的研究[J].计算机测量与控制,2016,24(5):71-73.Zhuang CF,Yang B,Niu JZ.Research of a bio-electrical impedance measurement based on multi-frequency technology[J].Computer Measurement&Control,2016,24(5):71-73.
[7]Ljungqvist O,Hedenborg G,Jacobson SH.Whole body impedance measurements reflect total body water changes.A study in hemodialysis patients[J].International Journal of Clinical Monitoring and Computing,1990,7(3):163-169.
[8]Souweine G,Lenoir J,Jenin P.Determination of body fluid compartments by impedance measurements:differences in the repartition of water between duchenne muscular dystrophy and some neurological diseases[J].Journal of Neurology,1981,226(3):199-203.
[9]苌飞霸,尹军,颜乐先,等.基于生物电阻抗法的人体成分测量系统的研究与评价[J].中国医学物理学杂志,2014,31(2):4833-4838.Chang FB,Yin J,Yan LX,et al.Research and evaluation on body composition measurement system based on bioelectrical impedance[J].Chinese Journal of Medical Physics,2014,31(2):4833-4838.
[10]云洁,王燕平,张维波,等.应用生物阻抗频谱法对循经体液分布的初步观察[J],中国中医基础医学杂志,2015,21(7):853-856.Yun J,Wang YP,Zhang WB,et al.Preliminary observation on the body fluid distribution along meridian using bioimpedance spectroscopy method[J].Journal of Basic Chinese Medicine,2015,21(7):853-856.
[11]杨威生.低阻经络研究IV对经络生理学功能的推测[J].北京大学学报(自然科学版),2008,44(2):281-288.Yang WS.Investigation of the lower resistance meridian IV.Speculation on the physiological functions of a cupuncture meridians[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2008,44(2):281-288.
[12]张维波,庄逢源,田宇瑛,等.使用凝胶模型法对经络生物物理特性的模拟研究[J].生物医学工程学杂志,2001,18(3):357-361.Zhang WB,Zhuang FY,Tian YY,et al.A simulating study of biophysical features a long meridians on a gel model[J].Journal of Biomedical Engineering,2001,18(3):357-361.
[13]张立剑,张维波,刘雨亭,等.人体电测量技术在经络研究中的运用[J].北京生物医学工程,2000,19(3):156-158.Zhang LJ,Zhang WB,Liu YT,et al.Application of human-body electrical detection techniqu ein meridian study of traditional Chinese medicine[J].Beijing Biomedical Engineering,2000,19(3):156-158.
[14]刘近贞.基于扫描电极的开放式电阻抗成像的研究[D].天津:天津大学,2014.Liu JZ.Study on the open electrical impedance tomography with scanning electrode[D].Tianjin:Tianjin University,2014.
[15]Kanai H,Sakamoto K,Haeno M.Electrical measurement of fluid distribution in human legs:estimation of extra-and intra-cellular fluid volume[J].Journal of Microwave Power,1983,18(3):233-243.