低盐胁迫对华贵栉孔扇贝抗氧化酶、Na~+/K~+-ATPase活力的影响
|
摘要
采用生物化学方法系统测定了低盐(18)胁迫下华贵栉孔扇贝(Mimachlamys nobilis)3种组织(肝脏、鳃、外套膜)的3种抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT、髓过氧化物酶MPO)活力、丙二醛MDA含量、Na~+/K~+-ATPase活力和肝脏糖原含量在3,6,9,12,24,48,72,96 h的动态变化,可为揭示华贵栉孔扇贝不同组织在低盐胁迫下的应激反应规律和免疫防御调节机制提供科学依据.结果表明:3种组织在低盐胁迫下的各种生理应答基本一致;3种抗氧化酶(SOD、CAT、MPO)活力随着胁迫时间的延长,呈现先增大后减小的趋势,一般在胁迫9或12 h到达最高值,随之降低逐渐恢复与起始时没有显著差异;MDA含量随胁迫时间延长持续上升;Na~+/K~+-ATPase活力随胁迫时间延长持续下降;肝脏糖原含量随胁迫时间延长持续下降.说明盐度下降后机体抗氧化系统受损,体内离子平衡被打破,能量供应不足,是导致华贵栉孔扇贝死亡的重要原因.因此在福建沿海选择其养殖区域应避免在洪水和台风季节盐度降至18的海区.
Under low-salinity(18) stress on Mimachlamys nobilis,activities of superoxide dismutase(SOD),catalase(CAT),myeloperoxidase(MPO),content of MDA,Na~+/K~+-ATPase activity in three tissues(liver,gill,mantle) and glycogen content in liver were analyzed and compared at 0,3,6,9,12,24,48,72,96 h by their corresponding biochemical methods. The results showed that physiological responses of three tissues were similar under low-salinity stress. Activities of three antioxidant enzymes(SOD,CAT,MPO) increased with extension of durature,reached their peak after 9 h or 12 h respectively,and then decreased. MDA content increased with duration,MDA content after 12 to 24 h compared to the initial level(0 h) had significant difference(P < 0. 05). Activity of Na~+/K~+-ATPase decreased with time elapse,activity after 3 to 12 h compared to the initial level had significant difference(P < 0. 05). Glycogen content in liver decreased with time elapse,glycogen content after 6 h compared to the initial level had significant difference(P < 0. 05). In conclusion,low-salinity stress would damage the antioxidant system,break the ion balance,have the insufficient energy supply,which were the important reasons leading to death of M. nobilis. The farming sites in Fujian coast should be avoided areas where the salinity falls to 18 in the flood and typhoon season.
Under low-salinity(18) stress on Mimachlamys nobilis,activities of superoxide dismutase(SOD),catalase(CAT),myeloperoxidase(MPO),content of MDA,Na~+/K~+-ATPase activity in three tissues(liver,gill,mantle) and glycogen content in liver were analyzed and compared at 0,3,6,9,12,24,48,72,96 h by their corresponding biochemical methods. The results showed that physiological responses of three tissues were similar under low-salinity stress. Activities of three antioxidant enzymes(SOD,CAT,MPO) increased with extension of durature,reached their peak after 9 h or 12 h respectively,and then decreased. MDA content increased with duration,MDA content after 12 to 24 h compared to the initial level(0 h) had significant difference(P < 0. 05). Activity of Na~+/K~+-ATPase decreased with time elapse,activity after 3 to 12 h compared to the initial level had significant difference(P < 0. 05). Glycogen content in liver decreased with time elapse,glycogen content after 6 h compared to the initial level had significant difference(P < 0. 05). In conclusion,low-salinity stress would damage the antioxidant system,break the ion balance,have the insufficient energy supply,which were the important reasons leading to death of M. nobilis. The farming sites in Fujian coast should be avoided areas where the salinity falls to 18 in the flood and typhoon season.
引文
[1]孙泽伟,郑怀平,张涛,等.低温和饥饿对华贵栉孔扇贝幼虫生长和存活的影响[J].生态学报,2010,30(1):1-6.
[2]谢仁政,梁飞龙.华贵栉孔扇贝规模化人工育苗技术[J].水产养殖,2009,130(6):21-22.
[3]王琦,袁涛,何毛贤.华贵栉孔扇贝养殖及遗传育种研究进展[J].南方水产科学,2011,7(5):73-80.
[4]LIU H,ZHENG H,WANG S,et al.Cloning and functional sharacterization of a poltunsaturated fattyidelongase in a marine bivalve noble scallop Chlamys nobilis Reeve[J].Aquaculture,2013,416(5):146-151.
[5]章启忠,刘志刚,王辉.华贵栉孔扇贝稚贝盐度适应性的研究[J].广东海洋大学学报,2008,28(1):40-43.
[6]郭春雨,管越强,刘波兰.温度、p H和盐度对克氏原螯虾鳃Na+/K+-ATPase活性的影响[J].动物学杂志,2008,42(6):96-102.
[7]尹飞,孙鹏,彭士明,等.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J].应用生态学报,2011,22(4):1059-1066.
[8]亢玉静,郎明远,赵文.水生生物体内抗氧化酶及其影响因素研究进展[J].微生物学杂志,2013,33(3):75-79.
[9]时少坤,王瑞璇,王江勇,等.盐度胁迫对近江牡蛎几种免疫因子的影响[J].南方水产科学,2013,9(3):26-30.
[10]徐永健,孙彬.盐度胁迫对大海马(Hippocampus kuda)幼体生长、组分及酶活力的影响[J].海洋与湖沼,2012,43(6):1279-1285.
[11]李子牛,林听听,么宗利,等.盐度对青蛤抗氧化酶活性及生长的影响[J].生态学杂志,2012,31(10):2625-2630.
[12]SSTYAVATHI C,RAO Y P.Inhibition of Na+/K+-ATPase in Penaeus indicus postlarvae by lead[J].Comparative Biochemistry and Physiology Part C,2000,127(1):11-22.
[13]林听听,来琦芳,陆建学,等.盐度突变对青蛤(Cyclina sinensis)鳃Na+/K+-ATPase活性的影响[J].广东海洋大学学报,2012,32(1):60-64.
[14]沈永龙,戈贤平,黄金田,等.盐度对瘤背石磺不同部位Na+/K+-ATPase酶活性、围心腔液和腹腔液渗透压及离子含量的影响[J].水产学报,2013,37(6):851-857.
[15]杨宇晴,余德光,谢骏,等.急性盐度胁迫对斜带石斑鱼Na+/K+-ATPase酶及血清应激指标的影响[J].热带海洋学报,2010,29(4):160-164.
[16]亓磊,蒋科技,顾孝连,等.盐度突降对拟穴青蟹大眼幼体生长发育和Na+/K+-ATPase活性的影响[J].海洋渔业,2011,33(2):201-205.
[17]刘美剑,常亚青,杨芸菲,等.盐度渐变对虾夷扇贝(Patinopecten yessoensis)免疫指标的影响[J].中国农业科技导报,2011,13(3):129-135.
[18]马洪明,刘晓伟,麦康森,等.盐度突降对栉孔扇贝(Chlamys farreri)抗病力指标的影响[J].高技术通讯,2006,16(7):746-751.
[19]刘志刚,刘建勇,杨博.温度与盐度对华贵栉孔扇贝幼贝存活与生长的互作效应研究[J].海洋科学,2011,35(10):75-80.
[20]李卫东,吕文刚,王辉,等.温度和盐度对华贵栉孔扇贝幼虫存活联合效应研究[J].水产养殖,2016,37(1):1-8.
[21]钱佳慧,栗志民,陈树河.温度和盐度对华贵栉孔扇贝幼贝淀粉酶和Na+/K+-ATP酶活性的联合效应[J].水产科学,2015,34(5):269-276.
[22]钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2016,11(6):49-57.
[23]栗志民,钱佳慧,劳翠英,等.温度和盐度对华贵栉孔扇贝免疫相关酶的联合效应[J].海洋科学进展,2015,33(2):227-238.
[24]孙金辉,徐赟霞,季延滨,等.温度骤降对南美白对虾仔虾抗氧化机能的影响[J].天津农学院学报,2008,15(3):7-10.
[25]潘鲁青,刘志,姜令绪.盐度,p H变化对凡纳滨对虾鳃丝Na+/K+-ATPase活力的影响[J].中国海洋大学学报(自然科学版),2004,34(5):787-790.
[26]潘鲁青,栾治华.盐度对日本囊对虾仔虾生长发育和Na+/K+-ATPase活力的影响[J].水生生物学报,2005,29(6):699-703.
[27]余德光,杨宇晴,王海英,等.盐度变化对斜带石斑鱼生理生化因子的影响[J].水产学报,2015,35(5):719-726.
[28]秦小明,章超桦,林华娟,等.净化菲律宾蛤仔低温保活及其主要营养成分变化[J].福建水产,2008(2):43-46.
[2]谢仁政,梁飞龙.华贵栉孔扇贝规模化人工育苗技术[J].水产养殖,2009,130(6):21-22.
[3]王琦,袁涛,何毛贤.华贵栉孔扇贝养殖及遗传育种研究进展[J].南方水产科学,2011,7(5):73-80.
[4]LIU H,ZHENG H,WANG S,et al.Cloning and functional sharacterization of a poltunsaturated fattyidelongase in a marine bivalve noble scallop Chlamys nobilis Reeve[J].Aquaculture,2013,416(5):146-151.
[5]章启忠,刘志刚,王辉.华贵栉孔扇贝稚贝盐度适应性的研究[J].广东海洋大学学报,2008,28(1):40-43.
[6]郭春雨,管越强,刘波兰.温度、p H和盐度对克氏原螯虾鳃Na+/K+-ATPase活性的影响[J].动物学杂志,2008,42(6):96-102.
[7]尹飞,孙鹏,彭士明,等.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J].应用生态学报,2011,22(4):1059-1066.
[8]亢玉静,郎明远,赵文.水生生物体内抗氧化酶及其影响因素研究进展[J].微生物学杂志,2013,33(3):75-79.
[9]时少坤,王瑞璇,王江勇,等.盐度胁迫对近江牡蛎几种免疫因子的影响[J].南方水产科学,2013,9(3):26-30.
[10]徐永健,孙彬.盐度胁迫对大海马(Hippocampus kuda)幼体生长、组分及酶活力的影响[J].海洋与湖沼,2012,43(6):1279-1285.
[11]李子牛,林听听,么宗利,等.盐度对青蛤抗氧化酶活性及生长的影响[J].生态学杂志,2012,31(10):2625-2630.
[12]SSTYAVATHI C,RAO Y P.Inhibition of Na+/K+-ATPase in Penaeus indicus postlarvae by lead[J].Comparative Biochemistry and Physiology Part C,2000,127(1):11-22.
[13]林听听,来琦芳,陆建学,等.盐度突变对青蛤(Cyclina sinensis)鳃Na+/K+-ATPase活性的影响[J].广东海洋大学学报,2012,32(1):60-64.
[14]沈永龙,戈贤平,黄金田,等.盐度对瘤背石磺不同部位Na+/K+-ATPase酶活性、围心腔液和腹腔液渗透压及离子含量的影响[J].水产学报,2013,37(6):851-857.
[15]杨宇晴,余德光,谢骏,等.急性盐度胁迫对斜带石斑鱼Na+/K+-ATPase酶及血清应激指标的影响[J].热带海洋学报,2010,29(4):160-164.
[16]亓磊,蒋科技,顾孝连,等.盐度突降对拟穴青蟹大眼幼体生长发育和Na+/K+-ATPase活性的影响[J].海洋渔业,2011,33(2):201-205.
[17]刘美剑,常亚青,杨芸菲,等.盐度渐变对虾夷扇贝(Patinopecten yessoensis)免疫指标的影响[J].中国农业科技导报,2011,13(3):129-135.
[18]马洪明,刘晓伟,麦康森,等.盐度突降对栉孔扇贝(Chlamys farreri)抗病力指标的影响[J].高技术通讯,2006,16(7):746-751.
[19]刘志刚,刘建勇,杨博.温度与盐度对华贵栉孔扇贝幼贝存活与生长的互作效应研究[J].海洋科学,2011,35(10):75-80.
[20]李卫东,吕文刚,王辉,等.温度和盐度对华贵栉孔扇贝幼虫存活联合效应研究[J].水产养殖,2016,37(1):1-8.
[21]钱佳慧,栗志民,陈树河.温度和盐度对华贵栉孔扇贝幼贝淀粉酶和Na+/K+-ATP酶活性的联合效应[J].水产科学,2015,34(5):269-276.
[22]钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2016,11(6):49-57.
[23]栗志民,钱佳慧,劳翠英,等.温度和盐度对华贵栉孔扇贝免疫相关酶的联合效应[J].海洋科学进展,2015,33(2):227-238.
[24]孙金辉,徐赟霞,季延滨,等.温度骤降对南美白对虾仔虾抗氧化机能的影响[J].天津农学院学报,2008,15(3):7-10.
[25]潘鲁青,刘志,姜令绪.盐度,p H变化对凡纳滨对虾鳃丝Na+/K+-ATPase活力的影响[J].中国海洋大学学报(自然科学版),2004,34(5):787-790.
[26]潘鲁青,栾治华.盐度对日本囊对虾仔虾生长发育和Na+/K+-ATPase活力的影响[J].水生生物学报,2005,29(6):699-703.
[27]余德光,杨宇晴,王海英,等.盐度变化对斜带石斑鱼生理生化因子的影响[J].水产学报,2015,35(5):719-726.
[28]秦小明,章超桦,林华娟,等.净化菲律宾蛤仔低温保活及其主要营养成分变化[J].福建水产,2008(2):43-46.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |