苦地丁的化学成分研究
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摘要
目的研究苦地丁乙醇提取物中的化学成分。方法利用萃取和硅胶柱层析分离和纯化苦地丁乙醇提取物,对分离得到的化合物进行理化性质表征和波谱结构鉴定。结果从苦地丁乙醇提取物中分离得到7个化合物,分别鉴定为:二氢血根碱(Ⅰ)、乙酰紫堇灵(Ⅱ)、血根碱(Ⅲ)、紫堇灵(Ⅳ)、原阿片碱(Ⅴ)、延胡索乙素(Ⅵ)、1,2,3,4,4α,9β-hexahydro-5-(4-methoxy-1,4-dioxobutyl)-2,2,8-trimethyl-,(4αS,9βR)-5H-pyrido[4,3-b]indolium(Ⅶ)。结论化合物Ⅶ为首次从苦地丁植物中分离得到。
OBJECTIVE To study the chemical constituents of the ethanol extract of Corydalis bungeana Turcz. METHODS The ethanol extract of C. bungeana was isolated and purified using various methods such as extraction and silica gel column chromatography. The physicochemical properties and spectral structure identification of the isolated compounds were performed.RESULTS Seven compounds were isolated from the ethanol extract of C. bungeana and identified as: Dihydrogen root base( Ⅰ),acetyl purpurin( Ⅱ),root alkaloids( Ⅲ),purpurin( Ⅳ),protopine( Ⅴ),tetrahydropalmatine( Ⅵ),1,2,3,4,4α,9β-hexahydro-5-( 4-methoxy-1,4-dioxobutyl)-2,2,8-trimethyl-,( 4αS,9βR)-5 H-pyrido[4,3-b]indolium.( Ⅶ). CONCLUSION Compound Ⅶ is isolated from this plant for the first time.
OBJECTIVE To study the chemical constituents of the ethanol extract of Corydalis bungeana Turcz. METHODS The ethanol extract of C. bungeana was isolated and purified using various methods such as extraction and silica gel column chromatography. The physicochemical properties and spectral structure identification of the isolated compounds were performed.RESULTS Seven compounds were isolated from the ethanol extract of C. bungeana and identified as: Dihydrogen root base( Ⅰ),acetyl purpurin( Ⅱ),root alkaloids( Ⅲ),purpurin( Ⅳ),protopine( Ⅴ),tetrahydropalmatine( Ⅵ),1,2,3,4,4α,9β-hexahydro-5-( 4-methoxy-1,4-dioxobutyl)-2,2,8-trimethyl-,( 4αS,9βR)-5 H-pyrido[4,3-b]indolium.( Ⅶ). CONCLUSION Compound Ⅶ is isolated from this plant for the first time.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1983:32.
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[3]肖扬,杨春娟,钟明亮,等.苦地丁化学成分研究(英文)[J].天然产物研究与开发,2013,25(12):1665-1668,1689.
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[5]易延逵,陈志良,邓虹珠.中药指纹图谱的研究评析[J].中成药,2006,28(8):1192-1197.
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[12]Nonaka G,Okabe H,Nishioka I,et al.Alkaloids of Corydalis incisa Pers.I.On the nonphenolic tertiary bases[J].Yakugaku Zasshi J Pharm Soc Japan,1973,93(1):87-93.
[13]Miao F,Yang XJ,Zhou L.Structural modification of sanguinarine and chelerythrine and their antibacterial activity[J].Natural Product Res,2011,25(9):863-875.
[14]Tou2ek J,Dommisse R,Dostál J,et al.Configurations and conformations of sanguinarine and chelerythrine free bases stereoisomers[J].J Mol Struct,2002,613(1-3):103-113.
[15]Tani C,Nagakura N,Nattori S,et al.Studies on the alk aloids of Papaveraceous plants XIX.Alkaloids of Corydalis koidzumiana[J].Ohwi Yakugaku Zasshi,1974,94(7):844-851.
[16]Takao N,Iwsa K,Kamigauchi M,et al.Studies on th e alkaloids of Papaveraceous plants XXX.Conformational analysis of some hydrobenzo[c]phenanth ridine-type alkaloids[J].Chem Pharm Bull,1978,26(6):1880-1889.
[17]Nonaka G,Okabe H,Nishioka I,et al.Alkaloids of Corydalis incisa Pers.I.On the nonphenolic tertiary bases[J].Yakugaku Zasshi,1973,93(1):87-93.
[18]Ma WG,Yukiharu F,Satoshi T.Fungitoxic alkaloids from Hokkaido Corydalis species[J].Fitoterapia,1999,70(3):258-265.
[19]Aggarwal P,Varshney VK,Dayal R,et al.Isoquinoline alkaloids from ethno botanically important Dicentra paucinervia[J].Int J Chem Sci,2009,7(3):1841-1850.
[20]许翔鸿,王峥涛,余国奠,等.延胡索中生物碱成分的研究[J].中国药科大学学报,2002,33(6):483-486.
[21]Sturm S,Strasser EM,Stuppner H.Quantification of fumaria officinalis isoquinoline alkaloids by nonaqueous capillary electrophoresis-electrospray ion trap mass spectrometry[J].JChromatog A,2006,1112(1-2):331-338.
[2]中华人民共和国国家药典委员会.中国药典[S].一部.北京:中国医药科技出版社,2015:201.
[3]肖扬,杨春娟,钟明亮,等.苦地丁化学成分研究(英文)[J].天然产物研究与开发,2013,25(12):1665-1668,1689.
[4]郑建芳,秦民坚,郑昱,等.苦地丁生物碱的化学成分[J].中国药科大学学报,2007,38(2):112-114.
[5]易延逵,陈志良,邓虹珠.中药指纹图谱的研究评析[J].中成药,2006,28(8):1192-1197.
[6]顾英,冯怡,李玉敏.指纹图谱在中药物质基础研究中的应用[J].中成药,2007,29(7):1048-1051.
[7]白雪梅,乔春林,黄冕,等.素心腊梅种子中生物碱成分的研究[J].华西药学杂志,2016,31(6):611-614.
[8]Tani C,Nagakura N,Nattori S,et al.Studies on the alkaloids of Papaveraceous plants.XIX.Alkaloids of Corydalis koidzumiana Ohwi[J].Yakugaku Zasshi,1974,94(7):844-851.
[9]Oechslin SM,K9nig GM,Oechslinmerkel K,et al.An nmr study of four benzophenanthr-idine alkaloids[J].J Natural Products,1991,54(54).519-524.
[10]Niu L,Xie Z,Cai T,et al.Preparative isolation of alkaloids from Corydalis bungeana Turcz.by high-speed countercurrent chromatography using stepwise elution[J].J Separation Sci,2011,34(9):987-994.
[11]Takao N,Iwasa K,Kamigauchi M,et al.Studies on the alkaloids of Papaveraceous plants.XXX.Conformational analysis of some hydro-benzo[c]phenanthridine-type alkaloids[J].Chem Pharm Bull,2008,26(6):1880-1889.
[12]Nonaka G,Okabe H,Nishioka I,et al.Alkaloids of Corydalis incisa Pers.I.On the nonphenolic tertiary bases[J].Yakugaku Zasshi J Pharm Soc Japan,1973,93(1):87-93.
[13]Miao F,Yang XJ,Zhou L.Structural modification of sanguinarine and chelerythrine and their antibacterial activity[J].Natural Product Res,2011,25(9):863-875.
[14]Tou2ek J,Dommisse R,Dostál J,et al.Configurations and conformations of sanguinarine and chelerythrine free bases stereoisomers[J].J Mol Struct,2002,613(1-3):103-113.
[15]Tani C,Nagakura N,Nattori S,et al.Studies on the alk aloids of Papaveraceous plants XIX.Alkaloids of Corydalis koidzumiana[J].Ohwi Yakugaku Zasshi,1974,94(7):844-851.
[16]Takao N,Iwsa K,Kamigauchi M,et al.Studies on th e alkaloids of Papaveraceous plants XXX.Conformational analysis of some hydrobenzo[c]phenanth ridine-type alkaloids[J].Chem Pharm Bull,1978,26(6):1880-1889.
[17]Nonaka G,Okabe H,Nishioka I,et al.Alkaloids of Corydalis incisa Pers.I.On the nonphenolic tertiary bases[J].Yakugaku Zasshi,1973,93(1):87-93.
[18]Ma WG,Yukiharu F,Satoshi T.Fungitoxic alkaloids from Hokkaido Corydalis species[J].Fitoterapia,1999,70(3):258-265.
[19]Aggarwal P,Varshney VK,Dayal R,et al.Isoquinoline alkaloids from ethno botanically important Dicentra paucinervia[J].Int J Chem Sci,2009,7(3):1841-1850.
[20]许翔鸿,王峥涛,余国奠,等.延胡索中生物碱成分的研究[J].中国药科大学学报,2002,33(6):483-486.
[21]Sturm S,Strasser EM,Stuppner H.Quantification of fumaria officinalis isoquinoline alkaloids by nonaqueous capillary electrophoresis-electrospray ion trap mass spectrometry[J].JChromatog A,2006,1112(1-2):331-338.