稻鸭共育模式下水稻根际土壤真菌和细菌种群多样性分析
|
摘要
【目的】比较稻鸭共育模式与常规管理模式种植水稻的根际土壤微生物种群多样性,为利用土壤微生物多样性开展水稻生态栽培提供科学依据。【方法】采集常规管理模式水稻根际土壤样品(记为S2)和稻鸭共育模式水稻根际土壤样品(记为S3),采用电位法测定土壤pH、重铬酸钾—外加热法测定土壤有机质含量;针对土壤样品真菌ITS1～ITS2区域和细菌16S rDNA扩增子V3～V4区域进行Illumina MiSeq 2×300 bp双末端测序,分析两种模式土壤真菌和细菌的种类及丰度差异。【结果】S3的p H为5.10,有机质含量为23.91 g/kg;S2的pH为5.34,有机质含量为19.84 g/kg。在真菌门水平上,两种管理模式的根际土壤优势门均为子囊菌门(Ascomycota),S3中子囊菌门的相对丰度为42.36%,S2为93.67%,两者相差2.21倍;在真菌属的水平上,S3的优势属为毛壳菌属(Chaetomium),相对丰度为2.63%,而S2的毛壳菌属相对丰度仅为0.26%,两者相差10.12倍;S3中居第2位的属是嗜热真菌属(Mycothermus),相对丰度为2.21%,而S2的嗜热真菌属相对丰度为0.05%,两者相差44.20倍;S2的优势属是黑盘孢属(Melanconiella),相对丰度为6.66%,而S3的黑盘孢属相对丰度为0.26%,两者相差25.62倍。两种管理模式下相对丰度居前2位的细菌门均为变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria),S3中变形菌门的相对丰度为40.90%,S2为29.45%,两者相差1.38倍;S3中酸杆菌门的相对丰度为18.90%,S2为27.84%,两者相差1.47倍;两种管理模式下相对丰度居前10的细菌属相同,其中7个属隶属变形菌门[其中3个属隶属于根瘤菌目(Rhizobiales)、3个属于隶属粘细菌目(Myxococcales)、1个属隶属于δ-变形菌纲未分目(unidentified Deltaproteobacteria)]、3个属隶属于酸杆菌门,10个属中仅有红微菌属(Rhodomicrobium)在两种管理模式土壤中相对丰度相差2.23倍(S3为1.18%,S2为0.53%),其他9个属相差均在1.00倍左右。【结论】与常规管理模式比较,稻鸭共育模式的水稻根际土壤pH略低,有机质稍高,真菌的种群结构丰富,而细菌的优势门和优势属无明显差异。
【Objective】The microbial population diversity in rhizosphere soil between rice-duck farming model and conventional management model in rice planting was compared in order to provide scientific basis on the utilization of soil microbial diversity to develop rice ecological cultivation.【Method】Rhizosphere soil samples from conventional management model(marked as S2)and from rice-duck farming model(marked as S3)were collected in the field,pH value of soil samples were tested by potential method,soil organic matter content was determined by potassium dichromate and external heating method. ITS1-ITS2 region of soil fungi and V3-V4 region of 16 S r DNA amplicon of bacteria in the soil samples were sequenced by Illumina MiSeq 2×300 bp double terminal sequencing method. Then fungi and bacteria species and abundance differences of soil samples in two cultivation models were analyzed.【Result】The pH value of S3 sample was 5.10,organic matter content was 23.91 g/kg;the pH value of S2 was 5.34,organic matter content was 19.84 g/kg.In fungi phyla level,Ascomycota was the dominant phylum in rice rhizosphere soil of two cultivation models,in which the relative abundance was 42.36% in S3 sample,but 93.67% in S2 sample,2.21 times in difference.In fungi genera level,Chaetomium was the dominant genus in S3 sample,the relative abundance was 2.63%. But in S2 sample,the relative abundance of Chaetomium was 0.26% only,10.12 times in difference. The second dominant genus in S3 sample was Mycothermus whose relative abundance was 2.21%,but in S2 sample,its relative abundance was 0.05%,44.20 times in difference.Melanconiella was the dominant genus in S2 sample whose relative abundance was 6.66%,but in S3 sample the relative abundance of Melanconiella was 0.26%,25.62 times in difference. In two management models,top two bacteria phyla were Proteobacteria and Acidobacteria,in which the relative abundance of Proteobacteria was 40.90% in S3 sample,29.45% in S2 sample,1.38 times in difference;the relative abundance of Acidobacteria is 18.90% in S3 sample,27.84% in S2 sample,1.47 times in difference. In two management models,the top ten bacteria genera were the same,in which seven genera belonged to Proteobacteria(among the seven genera,three genera belonged to Rhizobiales,three genera belonged to Myxococcales,one genus belonged to unidentified Deltaproteobacteria),and three genera belonged to Acidobacteria. Among ten genera,the relative abundance difference was about 1.00 time,only Rhodomicrobium was 2.23 times difference between two management models(the relative abundance was 1.18% in S3,0.53% in S2).【Conclusion】Compared with the conventional rice management model,the pH value is slightly lower,the organic matter is slightly higher in rice-duck farming model. Among soil samples from two management models,the population structure of fungi is abundant,and there is no great difference in dominant phyla and genera for bacteria.
【Objective】The microbial population diversity in rhizosphere soil between rice-duck farming model and conventional management model in rice planting was compared in order to provide scientific basis on the utilization of soil microbial diversity to develop rice ecological cultivation.【Method】Rhizosphere soil samples from conventional management model(marked as S2)and from rice-duck farming model(marked as S3)were collected in the field,pH value of soil samples were tested by potential method,soil organic matter content was determined by potassium dichromate and external heating method. ITS1-ITS2 region of soil fungi and V3-V4 region of 16 S r DNA amplicon of bacteria in the soil samples were sequenced by Illumina MiSeq 2×300 bp double terminal sequencing method. Then fungi and bacteria species and abundance differences of soil samples in two cultivation models were analyzed.【Result】The pH value of S3 sample was 5.10,organic matter content was 23.91 g/kg;the pH value of S2 was 5.34,organic matter content was 19.84 g/kg.In fungi phyla level,Ascomycota was the dominant phylum in rice rhizosphere soil of two cultivation models,in which the relative abundance was 42.36% in S3 sample,but 93.67% in S2 sample,2.21 times in difference.In fungi genera level,Chaetomium was the dominant genus in S3 sample,the relative abundance was 2.63%. But in S2 sample,the relative abundance of Chaetomium was 0.26% only,10.12 times in difference. The second dominant genus in S3 sample was Mycothermus whose relative abundance was 2.21%,but in S2 sample,its relative abundance was 0.05%,44.20 times in difference.Melanconiella was the dominant genus in S2 sample whose relative abundance was 6.66%,but in S3 sample the relative abundance of Melanconiella was 0.26%,25.62 times in difference. In two management models,top two bacteria phyla were Proteobacteria and Acidobacteria,in which the relative abundance of Proteobacteria was 40.90% in S3 sample,29.45% in S2 sample,1.38 times in difference;the relative abundance of Acidobacteria is 18.90% in S3 sample,27.84% in S2 sample,1.47 times in difference. In two management models,the top ten bacteria genera were the same,in which seven genera belonged to Proteobacteria(among the seven genera,three genera belonged to Rhizobiales,three genera belonged to Myxococcales,one genus belonged to unidentified Deltaproteobacteria),and three genera belonged to Acidobacteria. Among ten genera,the relative abundance difference was about 1.00 time,only Rhodomicrobium was 2.23 times difference between two management models(the relative abundance was 1.18% in S3,0.53% in S2).【Conclusion】Compared with the conventional rice management model,the pH value is slightly lower,the organic matter is slightly higher in rice-duck farming model. Among soil samples from two management models,the population structure of fungi is abundant,and there is no great difference in dominant phyla and genera for bacteria.
引文
鲍士旦.2000.土壤农化分析[M].第3版.北京:中国农业出版社.[Bao S D.2000.Soil and Agricultural Chemistry Analysis[M].The 3rdEdition.Beijing:China Agriculture Press.]
戴雷,张道环,于立繁,王文相,叶正和,张爱芳,陈雨.2011.水稻粒黑粉病研究进展[J].中国农学通报,27(12):261-265.[Dai L,Zhang D H,Yu L F,Wang W X,Ye Z H,Zhang A F,Chen Y.2011.Advance in rice kernel smut[J].Chinese Agricultural Science Bulletin,27(12):261-265.]
党雯,郜春花,张强,卢朝东,靳东升,李建华,卢晋晶.2014.解钾菌的研究进展及其在农业生产中的应用[J].山西农业科学,42(8):921-924.[Dang W,Gao C H,Zhang Q,Lu C D,Jin D S,Li J H,Lu J J.2014.Research progress of silicate bacteria and its application in agricultural production[J].Journal of Shanxi Agricultural Sciences,42(8):921-924.]
杜思瑶,于淼,刘芳华,肖雷雷,张洪霞,陶军,顾卫,顾京晏,陈茜.2017.设施种植模式对土壤细菌多样性及群落结构的影响[J].中国生态农业学报,25(11):1615-1625.[Du S Y,Yu M,Liu F H,Xiao L L,Zhang H X,Tao J,Gu W,Gu J Y,Chen X.2017.Effect of facility management regimes on soil bacterial diversity and community structure[J].Chinese Journal of Eco-Agriculture,25(11):1615-1625.]
郭云忠.2012.毛壳科Chaetomiaceae真菌多基因系统演化及分类鉴定研究[D].杨凌:西北农林科技大学.[Guo YZ.2012.The multi-gene phylogeny and classification of Chaetomiaceae[D].Yangling:Northwest A&F University.]
金崇伟,俞雪辉,郑绍建.2005.微生物在植物铁营养中的潜在作用[J].植物营养与肥料学报,11(5):688-695.[Jin C W,Yu X H,Zheng S J.2005.Latent function of microorganisms on plant iron acquisition[J].Plant Nutrition and Fertilizer Science,11(5):688-695.]
陆晓菊,官会林,张正芸,马永存,唐蜀昆.2015.三七连作根际土壤微生物区系的16S rRNA系统遗传多样性[J].微生物学报,55(2):205-213.[Lu X J,Guan H L,Zhang Z Y,Ma Y C,Tang S K.2015.Microbial distribution and 16S rRNA diversity in the rhizosphere soil of Panax notoginseng[J].Acta Microbiologica Sinica,55(2):205-213.]
吕仕琼,刘浩,赵江林,阴春晖,周立刚.2010.稻曲菌素研究进展[J].中国农学通报,26(14):265-268.[LüS Q,Liu H,Zhao J L,Yin C H,Zhou L G.2010.Research progress of ustiloxins[J].Chinese Agricultural Science Bulletin,26(14):265-268.]
潘彦硕,朱清禾,李聪,王幽静,吴坤,李烜桢,张世敏,吴宇澄.2018.纤维素、秸秆和木屑对农田土壤硝化作用及微生物的影响[J].河南农业大学学报,52(5):785-792.[Pan Y S,Zhu Q H,Li C,Wang Y J,Wu K,Li X Z,Zhang S M,Wu Y C.2018.Effects of cellulose,straw and sawdust on soil nitrification and microorganisms in farmland[J].Journal of Henan Agricultural University,52(5):785-792.]
逄好胜.2016.大兴安岭南坡植被退化对土壤微生物群落多样性的影响[D].哈尔滨:东北林业大学.[Pang H S.2016.Effects of forest degradation on soil microbial diversity in southern slope of Daxing’an Mountains[D].Harbin:Northeast Forestry University.]
乔志伟,洪坚平,方曌,李仕蓉.2017.溶磷细菌对复垦土壤有效磷及各形态无机磷含量的影响[J].河南农业科学,46(9):50-55.[Qiao Z W,Hong J P,Fang Z,Li S R.2017.Effect of phosphate-solubilizing bacteria on available phosphorus and various forms of inorganic phosphorus contents in reclaimed soils[J].Journal of Henan Agricultural Sciences,46(9):50-55.]
沈仁芳,赵学强.2015.土壤微生物在植物获得养分中的作用[J].生态学报,35(20):6584-6591.[Shen R F,Zhao XQ.2015.Role of soil microbes in the acquisition of nutrients by plants[J].Acta Ecologica Sinica,35(20):6584-6591.]
宋勇春,李晓林,冯固.2001.接种Glomus versiforme对红三叶草利用有机磷的影响[J].应用与环境生物学报,7(2):134-137.[Song Y C,Li X L,Feng G.2001.Effect of inoculated Glomus versiforme on utilization of organic phosphorus by red clover[J].Chinese Journal of Applied&Environmental Biology,7(2):134-137.]
谭悠久.2005.毛壳科Chaetomiaceae分类及分子系统发育研究[D].杨凌:西北农林科技大学.[Tan Y J.2005.Classification,identification and molecular phylogenetics of the family Chaetomiaceae[D].Yangling:Northwest A-griculture and Forestry University.]
王芳,图力古尔.2014.土壤真菌多样性研究进展[J].菌物研究,12(3):178-186.[Wang F,Bau Tolgor.2014.Research advances in the diversity of soil fungi[J].Journal of Fungal Research,12(3):178-186.]
王满,卫宣志,郭芳阳,李建华,张军召,张东峰.2017.动植物有机肥对烤烟根际土壤酶活性及微生物碳氮的影响[J].江西农业学报,29(10):63-66.[Wang M,Wei X Z,Guo F Y,Li J H,Zhang J Z,Zhang D F.2017.Effects of animal and plant organic fertilizer on enzymatic activity and microbial biomass carbon and nitrogen in rhizospheric soil of flue-cured[J].Acta Agriculturae Jiangxi,29(10):63-66.]
王轶,李季,曹志平,杨合法.2014.长期施肥对农田土壤真菌的影响[J].中国生态农业学报,22(11):1267-1273.[Wang Y,Li J,Cao Z P,Yang H F.2014.Effects of long-term fertilization on soil fungi[J].Chinese Journal of Eco-A-griculture,22(11):1267-1273.]
韦俊,杨焕文,徐照丽,邓小鹏,王戈,白羽祥,吕世保,谭小兵.2017.不同烤烟套作模式对烤烟根际土壤细菌群落特征及烤烟产质量的影响[J].南方农业学报,48(4):601-608.[Wei J,Yang H W,Xu Z L,Deng X P,Wang G,Bai Y X,LüS B,Tan X B.2017.Effects of different intercropping patterns on rhizosphere soil bacteria colony characteristics and flue-cured tobacco yield and quality[J].Journal of Southern Agriculture,48(4):601-608.]
谢联辉.2006.普通植物病理学[M].北京:科学出版社.[Xie L H.2006.General Plant Pathology[M].Beijing:Science Press.]
徐一兰,唐海明,肖小平,郭立君,李微艳,孙继民.2016.长期施肥对双季稻田土壤微生物学特性的影响[J].生态学报,36(18):5847-5855.[Xu Y L,Tang H M,Xiao X P,Guo L J,Li W Y,Sun J M.2016.Effects of different long-term fertilization regimes on the soil microbiological properties of a paddy field[J].Acta Ecologica Sinica,36(18):5847-5855.]
张薇,魏海雷,高洪文,胡跃高.2005.土壤微生物多样性及其环境影响因子研究进展[J].生态学杂志,24(1):48-52.[Zhang W,Wei H L,Gao H W,Hu Y G.2005.Advances of studies on soil microbial diversity and environmental impact factors[J].Chinese Journal of Ecology,24(1):48-52.]
周秀文.2013.土壤中粘细菌群落的调查及领地性行为的分子机制的研究[D].济南:山东大学.[Zhou X W.2013.Sub-population census for Myxobacteria in soil and molecular strategy research for their territoriality[D].Jinan:Shandong University.]
俎千惠,王保战,贾仲君,林先贵,冯有智.2016.水稻土中紫色光合细菌沿纬度梯度的空间分异特征[J].生态学报,36(21):6730-6737.[Zu Q H,Wang B Z,Jia Z J,Lin XG,Feng Y Z.2016.Spatial shifts in purple photosynthetic bacterial community composition in paddy soils along the latitude[J].Acta Ecologica Sinica,36(21):6730-6737.]
Basotra N,Kaur B,FalcoM D,Tsang A,Chadha B S.2016.Mycothermus thermophilus(Syn.Scytalidium thermophilum):Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed[J].Bioresource Technology,222:413-421.
Ferrer A,Miller A N,Shearer C A.2011.Minutisphaera and natipusilla:Two new genera of freshwater Dothideomycetes[J].Mycologia,103(2):411-423.
Ishaq S L,Johnson S P,Miller Z J,Lehnhoff E A,Olivo S,Yeoman C J,Menalled F D.2017.Impact of cropping systems,soil inoculum,and plant species identity on soil bacterial community structure[J].Microbial Ecology,73(2):417-434.
Raja H A,Oberlies N H,Figueroa M,Tanaka K,Hirayama K,Hashimoto A,Miller A N,Zelski S E,Shearer C A.2013.Freshwater Ascomycetes:Minutisphaera(Dothideomycetes)revisited,including one new species from Japan[J].Mycologia,105(4):959-976.
Senanayake I C,Crous P W,Groenewald J Z,Maharachchikumbura S S N,Jeewon R,Phillips A J L,Bhat J D,Perera R H,Li Q R,Li W J,Tangthirasunun N,Norphanphoun C,Karunarathna S C,Camporesi E,Manawasighe I S,AlSadi A M,Hyde K D.2017.Families of Diaporthales based on morphological and phylogenetic evidence[J],Studies in Mycology,86:217-296.
Voglmayr H,Rossman A Y,Castlebury L A,Jaklitsch W M.2012.Multigene phylogeny and taxonomy of the genus Melanconiella(Diaporthales)[J].Fungal Diversity,57(1):1-44.
Wang H,Li Z Y,Qiu H Y,Li S,Zhang T C.2018a.Biological degradation of aflatoxin B1 by Emericellopsis sp.1912 and Sarocladium sp.10A[J].Lecture Notes in Electrical Engineering,444:525-532.
Wang R Q,Xiao Y P,LüF J,Hu L Y,Wei L G,Yuan ZQ,Lin H X.2018b.Bacterial community structure and functional potential of rhizosphere soilsas influenced by nitrogen addition and bacterial wilt disease under continuous sesame cropping[J].Applied Soil Ecology,125:117-127.
Wikipedia.2018a.Ascomycota[EB/OL].https://en.wikipedia.org/wiki/Ascomycota.
Wikipedia.2018b.Chytridiomycota[EB/OL].https://en.wikipedia.org/wiki/Chytridiomycota.
Wikipedia.2018c.Glomeromycota[EB/OL].https://en.wikipedia.org/wiki/Glomeromycota.
Wikipedia.2018d.Rhodomicrobium[EB/OL].https://en.wikipedia.org/wiki/Rhodomicrobium.
戴雷,张道环,于立繁,王文相,叶正和,张爱芳,陈雨.2011.水稻粒黑粉病研究进展[J].中国农学通报,27(12):261-265.[Dai L,Zhang D H,Yu L F,Wang W X,Ye Z H,Zhang A F,Chen Y.2011.Advance in rice kernel smut[J].Chinese Agricultural Science Bulletin,27(12):261-265.]
党雯,郜春花,张强,卢朝东,靳东升,李建华,卢晋晶.2014.解钾菌的研究进展及其在农业生产中的应用[J].山西农业科学,42(8):921-924.[Dang W,Gao C H,Zhang Q,Lu C D,Jin D S,Li J H,Lu J J.2014.Research progress of silicate bacteria and its application in agricultural production[J].Journal of Shanxi Agricultural Sciences,42(8):921-924.]
杜思瑶,于淼,刘芳华,肖雷雷,张洪霞,陶军,顾卫,顾京晏,陈茜.2017.设施种植模式对土壤细菌多样性及群落结构的影响[J].中国生态农业学报,25(11):1615-1625.[Du S Y,Yu M,Liu F H,Xiao L L,Zhang H X,Tao J,Gu W,Gu J Y,Chen X.2017.Effect of facility management regimes on soil bacterial diversity and community structure[J].Chinese Journal of Eco-Agriculture,25(11):1615-1625.]
郭云忠.2012.毛壳科Chaetomiaceae真菌多基因系统演化及分类鉴定研究[D].杨凌:西北农林科技大学.[Guo YZ.2012.The multi-gene phylogeny and classification of Chaetomiaceae[D].Yangling:Northwest A&F University.]
金崇伟,俞雪辉,郑绍建.2005.微生物在植物铁营养中的潜在作用[J].植物营养与肥料学报,11(5):688-695.[Jin C W,Yu X H,Zheng S J.2005.Latent function of microorganisms on plant iron acquisition[J].Plant Nutrition and Fertilizer Science,11(5):688-695.]
陆晓菊,官会林,张正芸,马永存,唐蜀昆.2015.三七连作根际土壤微生物区系的16S rRNA系统遗传多样性[J].微生物学报,55(2):205-213.[Lu X J,Guan H L,Zhang Z Y,Ma Y C,Tang S K.2015.Microbial distribution and 16S rRNA diversity in the rhizosphere soil of Panax notoginseng[J].Acta Microbiologica Sinica,55(2):205-213.]
吕仕琼,刘浩,赵江林,阴春晖,周立刚.2010.稻曲菌素研究进展[J].中国农学通报,26(14):265-268.[LüS Q,Liu H,Zhao J L,Yin C H,Zhou L G.2010.Research progress of ustiloxins[J].Chinese Agricultural Science Bulletin,26(14):265-268.]
潘彦硕,朱清禾,李聪,王幽静,吴坤,李烜桢,张世敏,吴宇澄.2018.纤维素、秸秆和木屑对农田土壤硝化作用及微生物的影响[J].河南农业大学学报,52(5):785-792.[Pan Y S,Zhu Q H,Li C,Wang Y J,Wu K,Li X Z,Zhang S M,Wu Y C.2018.Effects of cellulose,straw and sawdust on soil nitrification and microorganisms in farmland[J].Journal of Henan Agricultural University,52(5):785-792.]
逄好胜.2016.大兴安岭南坡植被退化对土壤微生物群落多样性的影响[D].哈尔滨:东北林业大学.[Pang H S.2016.Effects of forest degradation on soil microbial diversity in southern slope of Daxing’an Mountains[D].Harbin:Northeast Forestry University.]
乔志伟,洪坚平,方曌,李仕蓉.2017.溶磷细菌对复垦土壤有效磷及各形态无机磷含量的影响[J].河南农业科学,46(9):50-55.[Qiao Z W,Hong J P,Fang Z,Li S R.2017.Effect of phosphate-solubilizing bacteria on available phosphorus and various forms of inorganic phosphorus contents in reclaimed soils[J].Journal of Henan Agricultural Sciences,46(9):50-55.]
沈仁芳,赵学强.2015.土壤微生物在植物获得养分中的作用[J].生态学报,35(20):6584-6591.[Shen R F,Zhao XQ.2015.Role of soil microbes in the acquisition of nutrients by plants[J].Acta Ecologica Sinica,35(20):6584-6591.]
宋勇春,李晓林,冯固.2001.接种Glomus versiforme对红三叶草利用有机磷的影响[J].应用与环境生物学报,7(2):134-137.[Song Y C,Li X L,Feng G.2001.Effect of inoculated Glomus versiforme on utilization of organic phosphorus by red clover[J].Chinese Journal of Applied&Environmental Biology,7(2):134-137.]
谭悠久.2005.毛壳科Chaetomiaceae分类及分子系统发育研究[D].杨凌:西北农林科技大学.[Tan Y J.2005.Classification,identification and molecular phylogenetics of the family Chaetomiaceae[D].Yangling:Northwest A-griculture and Forestry University.]
王芳,图力古尔.2014.土壤真菌多样性研究进展[J].菌物研究,12(3):178-186.[Wang F,Bau Tolgor.2014.Research advances in the diversity of soil fungi[J].Journal of Fungal Research,12(3):178-186.]
王满,卫宣志,郭芳阳,李建华,张军召,张东峰.2017.动植物有机肥对烤烟根际土壤酶活性及微生物碳氮的影响[J].江西农业学报,29(10):63-66.[Wang M,Wei X Z,Guo F Y,Li J H,Zhang J Z,Zhang D F.2017.Effects of animal and plant organic fertilizer on enzymatic activity and microbial biomass carbon and nitrogen in rhizospheric soil of flue-cured[J].Acta Agriculturae Jiangxi,29(10):63-66.]
王轶,李季,曹志平,杨合法.2014.长期施肥对农田土壤真菌的影响[J].中国生态农业学报,22(11):1267-1273.[Wang Y,Li J,Cao Z P,Yang H F.2014.Effects of long-term fertilization on soil fungi[J].Chinese Journal of Eco-A-griculture,22(11):1267-1273.]
韦俊,杨焕文,徐照丽,邓小鹏,王戈,白羽祥,吕世保,谭小兵.2017.不同烤烟套作模式对烤烟根际土壤细菌群落特征及烤烟产质量的影响[J].南方农业学报,48(4):601-608.[Wei J,Yang H W,Xu Z L,Deng X P,Wang G,Bai Y X,LüS B,Tan X B.2017.Effects of different intercropping patterns on rhizosphere soil bacteria colony characteristics and flue-cured tobacco yield and quality[J].Journal of Southern Agriculture,48(4):601-608.]
谢联辉.2006.普通植物病理学[M].北京:科学出版社.[Xie L H.2006.General Plant Pathology[M].Beijing:Science Press.]
徐一兰,唐海明,肖小平,郭立君,李微艳,孙继民.2016.长期施肥对双季稻田土壤微生物学特性的影响[J].生态学报,36(18):5847-5855.[Xu Y L,Tang H M,Xiao X P,Guo L J,Li W Y,Sun J M.2016.Effects of different long-term fertilization regimes on the soil microbiological properties of a paddy field[J].Acta Ecologica Sinica,36(18):5847-5855.]
张薇,魏海雷,高洪文,胡跃高.2005.土壤微生物多样性及其环境影响因子研究进展[J].生态学杂志,24(1):48-52.[Zhang W,Wei H L,Gao H W,Hu Y G.2005.Advances of studies on soil microbial diversity and environmental impact factors[J].Chinese Journal of Ecology,24(1):48-52.]
周秀文.2013.土壤中粘细菌群落的调查及领地性行为的分子机制的研究[D].济南:山东大学.[Zhou X W.2013.Sub-population census for Myxobacteria in soil and molecular strategy research for their territoriality[D].Jinan:Shandong University.]
俎千惠,王保战,贾仲君,林先贵,冯有智.2016.水稻土中紫色光合细菌沿纬度梯度的空间分异特征[J].生态学报,36(21):6730-6737.[Zu Q H,Wang B Z,Jia Z J,Lin XG,Feng Y Z.2016.Spatial shifts in purple photosynthetic bacterial community composition in paddy soils along the latitude[J].Acta Ecologica Sinica,36(21):6730-6737.]
Basotra N,Kaur B,FalcoM D,Tsang A,Chadha B S.2016.Mycothermus thermophilus(Syn.Scytalidium thermophilum):Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed[J].Bioresource Technology,222:413-421.
Ferrer A,Miller A N,Shearer C A.2011.Minutisphaera and natipusilla:Two new genera of freshwater Dothideomycetes[J].Mycologia,103(2):411-423.
Ishaq S L,Johnson S P,Miller Z J,Lehnhoff E A,Olivo S,Yeoman C J,Menalled F D.2017.Impact of cropping systems,soil inoculum,and plant species identity on soil bacterial community structure[J].Microbial Ecology,73(2):417-434.
Raja H A,Oberlies N H,Figueroa M,Tanaka K,Hirayama K,Hashimoto A,Miller A N,Zelski S E,Shearer C A.2013.Freshwater Ascomycetes:Minutisphaera(Dothideomycetes)revisited,including one new species from Japan[J].Mycologia,105(4):959-976.
Senanayake I C,Crous P W,Groenewald J Z,Maharachchikumbura S S N,Jeewon R,Phillips A J L,Bhat J D,Perera R H,Li Q R,Li W J,Tangthirasunun N,Norphanphoun C,Karunarathna S C,Camporesi E,Manawasighe I S,AlSadi A M,Hyde K D.2017.Families of Diaporthales based on morphological and phylogenetic evidence[J],Studies in Mycology,86:217-296.
Voglmayr H,Rossman A Y,Castlebury L A,Jaklitsch W M.2012.Multigene phylogeny and taxonomy of the genus Melanconiella(Diaporthales)[J].Fungal Diversity,57(1):1-44.
Wang H,Li Z Y,Qiu H Y,Li S,Zhang T C.2018a.Biological degradation of aflatoxin B1 by Emericellopsis sp.1912 and Sarocladium sp.10A[J].Lecture Notes in Electrical Engineering,444:525-532.
Wang R Q,Xiao Y P,LüF J,Hu L Y,Wei L G,Yuan ZQ,Lin H X.2018b.Bacterial community structure and functional potential of rhizosphere soilsas influenced by nitrogen addition and bacterial wilt disease under continuous sesame cropping[J].Applied Soil Ecology,125:117-127.
Wikipedia.2018a.Ascomycota[EB/OL].https://en.wikipedia.org/wiki/Ascomycota.
Wikipedia.2018b.Chytridiomycota[EB/OL].https://en.wikipedia.org/wiki/Chytridiomycota.
Wikipedia.2018c.Glomeromycota[EB/OL].https://en.wikipedia.org/wiki/Glomeromycota.
Wikipedia.2018d.Rhodomicrobium[EB/OL].https://en.wikipedia.org/wiki/Rhodomicrobium.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |