杏仁油挥发性成分指纹图谱的构建及掺伪定量检测方法研究
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摘要
以17种不同的杏仁油为原料,采用顶空固相微萃取(SPME)和气相色谱仪测定了其挥发性成分,建立了杏仁油挥发性成分指纹图谱,并采用向量夹角余弦法计算了指纹图谱相似度。结果表明,杏仁油挥发性成分指纹图谱由12个共有色谱峰构成,通过聚类分析和相似度分析,发现该指纹图谱重复性好,专属性强。在该方法的基础上,建立了菜籽油-杏仁油掺伪模型:Y=-2947.0X~3+1254.9X~2+77.661X+46.693(其中Y为菜籽油掺假比例%,X为夹角余弦cosθ),适用于掺伪量15%以上杏仁油的鉴别。
To build a fingerprint based on volatile component of apricot kernel oil,we used the headspace solid phase microextraction( SPME) and GC method to determinate the volatile components of 17 kinds of different apricot kernel oil,and the fingerprint similarity was calculated by vector included angle cosine method.The results showed that the apricot kernel oil fingerprint of volatile component consisted of 12 common chromatographic peaks,and the fingerprint was of good repeatability and strong specificity according to the similarity and cluster analysis.Based on the method,we built the adulteration model for rapeseed oil,apricot kernel oil which was that: Y =-2947.0X~3+ 1254.9X~2+77.661 X + 46.693( Y for rapeseed oil adulteration ratio(%),X for cosθ),and this model could be applied to the identification of more than 15% of the amount of adulteration apricot kernel oil.
To build a fingerprint based on volatile component of apricot kernel oil,we used the headspace solid phase microextraction( SPME) and GC method to determinate the volatile components of 17 kinds of different apricot kernel oil,and the fingerprint similarity was calculated by vector included angle cosine method.The results showed that the apricot kernel oil fingerprint of volatile component consisted of 12 common chromatographic peaks,and the fingerprint was of good repeatability and strong specificity according to the similarity and cluster analysis.Based on the method,we built the adulteration model for rapeseed oil,apricot kernel oil which was that: Y =-2947.0X~3+ 1254.9X~2+77.661 X + 46.693( Y for rapeseed oil adulteration ratio(%),X for cosθ),and this model could be applied to the identification of more than 15% of the amount of adulteration apricot kernel oil.
引文
[1]张慧敏,孙容芳,于同泉,等.超临界CO2流体萃取杏仁油的研究[J].粮油食品科技,2001,1(9):29-41.
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[19]喻凤香,林亲录,黄中培,等.基于指纹图谱相似度的米糠油掺伪检测方法研究[J].中国粮油学报,2013,28(10):118-122.
[20]Seo H Y,Ha J,Shin D B,et al.Detection of corn oil in adulterated sesame oil by chromatography and carbon isotope analysis[J].Journal of the American Oil Chemists’Society,2010,87(6):621-626.
[21]国家药品监督管理局.关于印发《中药注射剂指纹图谱研究的技术要求(暂行)》的通知[J].中成药,2000,22(10):671.
[22]邓冠男.聚类分析中的相似度研究[J].东北电力大学学报,2013(1):156-161.
[23]关洪月,李林,刘晓,等.中药指纹图谱相似度计算方法探析[J].中国实验方剂学杂志,2011,17(18):282-287.
[24]谷瑞敏,涂洪谊,孙鹤.中药色谱指纹图谱相似度计算方法的探讨[J].中成药,2009,31(7):988-990.
[2]中国科学院西北植物研究所编.秦岭植物志第一卷(第二册)[M].科学出版社,1974.
[3]赵文彬,刘金荣,但建明,等.用均匀设计法优选苦杏仁油的超声提取工艺[J].中国中药杂志,2002,27(4):303-304.
[4]张华,于淼.仁用杏发展及综合利用现状与潜力[J].辽宁农业科学,2005,6:40-42.
[5]林丽敏.气相色谱法测定芝麻油掺伪的研究[J].粮食储藏,2006,35(3):43-45.
[6]黄永辉,钟海雁,李忠海.固相微萃取及其在食用植物油香气研究中的应用[J].食品研究与开发,2006,27(8):192-196.
[7]吴继红,胡小松,周珊,等.固相微萃取和气-质联用技术在快速测定苹果中挥发性成分中的应用[J].饮料工业,2003,6(3):39-41.
[8]邢丽红,冷凯良,孙伟红,等.气相色谱法检测鱼和虾中复硝酚钠残留量[J].中山大学学报:自然科学版,2013,52(6):104-109.
[9]陈俊卿,王锡昌.顶空固相微萃取-气相色谱-质谱法分析白鲢鱼中的挥发性成分[J].质谱学报,2005,26(2):76-80.
[10]胡国栋,张晓磊.顶空固相微萃取-气相色谱/质谱分析啤酒微量香味组分的研究[J].食品与发酵工业,2004,30(2):1-5.
[11]Mildner-Szkudlarz S,JeleńH H.The potential of different techniques for volatile compounds analysis coupled with PCA for the detection of the adulteration of olive oil with hazelnut oil[J].Food Chemistry,2008,110(3):751-761.
[12]秦早,杨冉,高桂园,等.顶空固相微萃取结合气质联用分析芝麻油和芝麻香精的挥发性成分[J].食品科学,2012,33(24):263-268.
[13]Sanahuja AB,Santonja MR,Teruel NG,et al.Classification of almond cultivars using oil volatile compound determination by HS-SPME-GC-MS[J].Journal of the American Oil Chemists’Society,2011,88(3):329-336.
[14]陈辉,汤道权,戚建良,等.基于指纹图谱技术快速检测掺伪花生油的方法探究[J].安徽农业科学,2014,42(22):7325-7326.
[15]黄娇丽.山茶油掺伪检验方法研究[D].南宁:广西大学,2014.
[16]喻凤香.米糠油指纹图谱的构建及掺伪定量检测技术研究[J].中国油脂,2015,40(2):61-65.
[17]李雪琴,黎海红,苗笑亮.指纹图谱相似度在芝麻油掺伪检测中的应用[J].食品科技,2009,34(3):259-264.
[18]王明清.食用油气相色谱指纹图谱鉴定技术及其应用研究[D].北京:中国农业科学院,2011.
[19]喻凤香,林亲录,黄中培,等.基于指纹图谱相似度的米糠油掺伪检测方法研究[J].中国粮油学报,2013,28(10):118-122.
[20]Seo H Y,Ha J,Shin D B,et al.Detection of corn oil in adulterated sesame oil by chromatography and carbon isotope analysis[J].Journal of the American Oil Chemists’Society,2010,87(6):621-626.
[21]国家药品监督管理局.关于印发《中药注射剂指纹图谱研究的技术要求(暂行)》的通知[J].中成药,2000,22(10):671.
[22]邓冠男.聚类分析中的相似度研究[J].东北电力大学学报,2013(1):156-161.
[23]关洪月,李林,刘晓,等.中药指纹图谱相似度计算方法探析[J].中国实验方剂学杂志,2011,17(18):282-287.
[24]谷瑞敏,涂洪谊,孙鹤.中药色谱指纹图谱相似度计算方法的探讨[J].中成药,2009,31(7):988-990.