一测多评法同时测定降脂减肥保健品中儿茶素类活性成分的含量
|
摘要
目的:建立同时测定减肥降脂保健品中儿茶素类活性成分含量的方法。方法:采用高效液相色谱法,以表没食子基儿茶素没食子酸酯(EGCG)为内标,计算其与没食子儿茶素(EGC)、儿茶素(C)、表儿茶素(EC)、没食子基儿茶素没食子酸酯(GCG)和没食子酰表儿茶素(ECG)的相对校正因子(RCF),通过RCF计算样品中EGC、C、EC、GCG、ECG的含量,以外标法测定的EGC、C、EC、GCG和ECG含量作实测值;采用向量夹角余弦法评估实测值与计算值之间的相似性。结果:EGC、C、EC、EGCG、GCG、ECG检测质量浓度线性范围分别为0.006 5~0.130 5 mg/m L(r=0.999 8)、0.000 5~0.010 7 mg/m L(r=0.999 7)、0.002 0~0.040 0mg/m L(r=0.999 9)、0.015 3~0.305 3 mg/m L(r=0.999 8)、0.000 8~0.015 5 mg/m L(r=0.999 8)、0.004 0~0.079 2 mg/m L(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为95.07%~100.35%(RSD=1.94%,n=6)、95.24%~101.87%(RSD=2.79%,n=6)、96.08%~103.86%(RSD=3.01%,n=6)、97.51%~101.06%(RSD=1.45%,n=6)、96.01%~101.66%(RSD=2.27%,n=6)、96.20%~102.89%(RSD=2.71%,n=6)。实测值与计算值差异无统计学意义(P>0.05)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于减肥降脂保健品中儿茶素类活性成分的含量测定。
OBJECTIVE:To establish the method for contents determination of catechins active components in lipid-lowering slimming health products. METHODS:HPLC method was adopted. Using epigallocatechin gallate(EGCG)as a reference,relative correction factor(RCF)of EGCG to gallocatechin(EGC),catechin(C),epicatechin(EC),gallocatechin gallate(GCG)and galloylepicatechin(ECG)were calculated. The contents of EGC,C,EC,GCG and ECG in 5 batches of samples were calculated through RCF. The contents of EGC,C,EC,GCG and ECG determined by external standard method were used as measured value.The similarity of the value determined by external standard method with the value calculated by quantitative analysis of multi-components via single marker method(QAMS)was evaluated with vector included angle cosine method. RESULTS:The linear ranges of EGCG,EGC,C,EC,GCG and ECG were 0.006 5-0.130 5 mg/m L(r=0.999 8)、0.000 5-0.010 7 mg/m L(r=0.999 7)、0.002 0-0.040 0 mg/m L(r=0.999 9)、0.015 3-0.305 3 mg/m L(r=0.999 8)、0.000 8-0.015 5 mg/m L(r=0.999 8)、0.004 0-0.079 2 mg/m L(r=0.999 9);RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.07%-100.35%(RSD=1.94%,n=6)、95.24%-101.87%(RSD=2.79%,n=6)、96.08%-103.86%(RSD=3.01%,n=6)、97.51%-101.06%(RSD=1.45%,n=6)、96.01%-101.66%(RSD=2.27%,n=6)、96.20%-102.89%(RSD=2.71%,n=6),respectively. There was no significant difference between measured value and calculated value. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for contents determination of catechins active components in lipid-lowering slimming health products.
OBJECTIVE:To establish the method for contents determination of catechins active components in lipid-lowering slimming health products. METHODS:HPLC method was adopted. Using epigallocatechin gallate(EGCG)as a reference,relative correction factor(RCF)of EGCG to gallocatechin(EGC),catechin(C),epicatechin(EC),gallocatechin gallate(GCG)and galloylepicatechin(ECG)were calculated. The contents of EGC,C,EC,GCG and ECG in 5 batches of samples were calculated through RCF. The contents of EGC,C,EC,GCG and ECG determined by external standard method were used as measured value.The similarity of the value determined by external standard method with the value calculated by quantitative analysis of multi-components via single marker method(QAMS)was evaluated with vector included angle cosine method. RESULTS:The linear ranges of EGCG,EGC,C,EC,GCG and ECG were 0.006 5-0.130 5 mg/m L(r=0.999 8)、0.000 5-0.010 7 mg/m L(r=0.999 7)、0.002 0-0.040 0 mg/m L(r=0.999 9)、0.015 3-0.305 3 mg/m L(r=0.999 8)、0.000 8-0.015 5 mg/m L(r=0.999 8)、0.004 0-0.079 2 mg/m L(r=0.999 9);RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.07%-100.35%(RSD=1.94%,n=6)、95.24%-101.87%(RSD=2.79%,n=6)、96.08%-103.86%(RSD=3.01%,n=6)、97.51%-101.06%(RSD=1.45%,n=6)、96.01%-101.66%(RSD=2.27%,n=6)、96.20%-102.89%(RSD=2.71%,n=6),respectively. There was no significant difference between measured value and calculated value. CONCLUSIONS:The method is simple,precise,stable and reproducible,and can be used for contents determination of catechins active components in lipid-lowering slimming health products.
引文
[1]赵振军,刘宗岸,仓梅琴,等.茶多酚及儿茶素对人体健康的影响[J].福建茶叶,2006,39(1):38-41.
[2]李学鸣,孟宪军,彭杰.茶多酚降低小鼠营养性肥胖的效果[J].沈阳农业大学学报,2009,40(5):612-614.
[3]丁友昌.分光光度法测定茶多酚保健品中茶多酚[J].中国公共卫生,1998,14(9):573-575.
[4]袁和军,邱明丰.茶多酚检测方法综述[J].北方药学,2013,10(11):11-13.
[5]刘振,余梦瑶,胡丹,等.好易升颗粒剂的含量测定及稳定性研究[J].华西药学杂志,2016,31(4):422-425.
[6]左岚,孟胜男.一测多评法在中药药物分析中的应用进展[J].中国药房,2016,27(18):2589-2591.
[7]徐彦,刘圆,吕露阳,等.一测多评法测定卷叶贝母中生物碱类成分含量[J].天然产物研究与开发,2012,24(11):1513-1515.
[8]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-659.
[9]王龙星,肖红斌,梁鑫淼,等.一种评价中药色谱指纹谱相似性的新方法:向量夹角法[J].药学学报,2002,37(9):713-715.
[2]李学鸣,孟宪军,彭杰.茶多酚降低小鼠营养性肥胖的效果[J].沈阳农业大学学报,2009,40(5):612-614.
[3]丁友昌.分光光度法测定茶多酚保健品中茶多酚[J].中国公共卫生,1998,14(9):573-575.
[4]袁和军,邱明丰.茶多酚检测方法综述[J].北方药学,2013,10(11):11-13.
[5]刘振,余梦瑶,胡丹,等.好易升颗粒剂的含量测定及稳定性研究[J].华西药学杂志,2016,31(4):422-425.
[6]左岚,孟胜男.一测多评法在中药药物分析中的应用进展[J].中国药房,2016,27(18):2589-2591.
[7]徐彦,刘圆,吕露阳,等.一测多评法测定卷叶贝母中生物碱类成分含量[J].天然产物研究与开发,2012,24(11):1513-1515.
[8]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-659.
[9]王龙星,肖红斌,梁鑫淼,等.一种评价中药色谱指纹谱相似性的新方法:向量夹角法[J].药学学报,2002,37(9):713-715.