黑松针叶束水培生根研究
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摘要
以黑松针叶束为试材,研究母树年龄、采叶时间、生长调节剂处理及营养液对针叶束水培生根的影响。结果表明:2 a生母树针叶束生根率最高;2月份为最佳采叶时间;最适的生长调节剂为200 mg/L IAA+IBA(1∶1),处理15 h;营养液配方以H_3BO_3 50 mg/L+NH_4NO_3 60 mg/L+VB_1 30 mg/L+KH_2PO_4 40 mg/L为最佳。
Using the needle fascicles of Pinus thunbergii as the test material,the effects of different mother tree ages,exogenous hormones,the leaf picking time and culture solutions on the rooting of needle fascicles were investigated.The results showed that the highest rooting rate was obtained with two-year-old tree needle fascicles.February was the best leaf picking time.The suitable rooting exogenous was IAA+IBA( 1∶1) with the concentration of 200 mg/L to treat the needle fascicles for 15 h. The optimum culture solution was H_3BO_3( 50 mg/L) +NH_4NO_3( 60 mg/L) +VB_1( 30 mg/L) +KH_2PO_4( 40 mg/L).
Using the needle fascicles of Pinus thunbergii as the test material,the effects of different mother tree ages,exogenous hormones,the leaf picking time and culture solutions on the rooting of needle fascicles were investigated.The results showed that the highest rooting rate was obtained with two-year-old tree needle fascicles.February was the best leaf picking time.The suitable rooting exogenous was IAA+IBA( 1∶1) with the concentration of 200 mg/L to treat the needle fascicles for 15 h. The optimum culture solution was H_3BO_3( 50 mg/L) +NH_4NO_3( 60 mg/L) +VB_1( 30 mg/L) +KH_2PO_4( 40 mg/L).
引文
[1]Ticknor R L.Review of the rooting of pines[J].Proceedings of the International Plant Propagators Society,1969,19(1):132-137.
[2]Boc A A,Steward R B,Banko T J.Effects of sedum leaf cutting[J].Hort Science,1972,74(4):404-405.
[3]周心铁,杨承桂.针叶束育苗技术[M].北京:中国林业出版社,1985:6.Zhou X T,Yang C J.Seedling technique of needle fascicles[M].Beijing:China Forestry Publishing House,1985:6.
[4]于世河.我国针叶树水培技术研究进展[J].辽宁林业科技,2014,2(2):60-62.Yu S H.Research progress of conifer hydroponic technology in China[J].Forestry Science and Technology of Liaoning Province,2014,2(2):60-62.
[5]王以珊,韦战.马尾松湿地松针叶束育苗试验小结[J].广东林业科技,1988,1(1):18,29.Wang Y S,Wei Z.Needle fascicles seedling experiment summary of Pinus elliottii and Pinus massoniana[J].Forestry Science and Technology of Guangdong Province,1988,1(1):18,29
[6]杨承桂,周全国,赵文平等.湿地松无性系针叶束育苗生根的研究[J].湖北林业科技,1990,73(3):21-24,34.Yang C J,Zhou Q G,Zhao W P,et al.Rooting of needle fascicles of Pinus elliottii.[J].Forestry Science and Technology of Hubei Province,1990,73(3):21-24,34.
[7]唐德瑞,张成高.油松针叶束离体培养及植株重建研究[J].中国科技论文在线,2008,4(21):1-7.Tang D R,Zhang C G.Propagation of pinus tabulaeformis from needle fascicles[J].Science Paper Online of China,2008,4(21):1-7.
[8]李福秀,张晓明,周体林,等.思茅松针叶束水培生根的研究[J].西南林学院报,2005,25(1):25-27.Li F X,Zhang X M,Zhou T L,et al.Rooting experiment with needle aquiculture of Pinus kesiya var.langbianensis[J].Journal of Southwest Forestry College,2005,25(1):25-27.
[9]吴敏,宋西德,周锋利,等.柴松针叶束水培生根的研究[J].西北林学院学报,2009,24(2):60-62.Wu M,Song X D,Zhou F L,et al.Rooting of needle fascicles of Pinus tabulaeformis f.shekannesis by Water[J].Culture Journal of Northwest Forestry University,2009,24(2):60-62.
[10]陶锡岗,矫德胜.黑松针叶束育苗技术试验报告[J].山东林业科技,1985,55(2):28-31.Tao X G,Jiao D S.Needle fascicles seedling experiment summary of Pinus thunbergii[J].Journal of Shandong Forestry Science and Technology,1985,55(2):28-31.
[11]森下义郎.植物扦插理论与技术[M].李云森译.北京:中国林业出版社,1988:8.Morishita Yoshiro.Theory and technology of plant cutting[M].Li Y S translate.Beijing:China Forestry Publishing House,1988:8.
[12]汪天,许成林,何云核.黄刺玫插穗生根能力及其机理的探讨[J].安徽农业大学学报,2000,27(3):221-224.Wang T,Xu C L,He Y H.Studies on rooting capability and mechanism of cuttings of Rosa xanthine[J].Journal of Anhui Agricultural University,2000,27(3):221-224.
[13]车少辉.油松针叶束水培生根技术研究[D].杨凌:西北农林科技大学,2008.Che S H.Rooting of needle fascicles of Pinus tabulaeformis by water culture[D].Yangling:Northwest A&F University,2008.
[14]Bartel B,Le Clere S,Magidin M,et al.Inputs to the active indole-3-acetic acid pool:de novo synthesis,conjugate hydrolysis,and indole-3-butyric acidβ-oxidation[J].Journal of Plant Growth Regulation,2001,20(3):198-216.
[15]Nordstr m A C,Jacobs F A,Eliasson L.Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings[J].Plant Physiology,1991,96(3):856-861.
[16]Singh,K K,Gurung B.In vitro propagation of R.maddeni Hook.F.an endangered rhododendron species of Sikkim Himalaya[J].Notulae Botanicae Horti Agrobotanici Cluj-Napoca,2009,37(1):79-83.
[17]Chen X J,Zhou Y,Zhang Q X,et al.Cutting propagation of wild Rhododendron decorum Franch[J].Hort Science,2009,44(4):1104.
[18]Bashir M A,Anjum M A,Chaudhry Z,et al.Response of jojoba(Simmondsia chinensis)cuttings to various concentrations of auxins[J].Pak J Bot,2009,41(6):2831-2840.
[19]Rana R S,Sood K K.Effect of cutting diameter and hormonal application on the propagation of Ficus roxburghii wall.Through branch cuttings[J].Annals of Forest Research,2012,55(1):69-84.
[20]吴惠珊,张华通,赵奋成,等.湿加松针叶束水培生根研究[J].广东林业科技,2012,28(6):20-26.Wu H S,Zhang H T,Zhao F C,et al.Rooting of needle fascicles of Pinus elliottii×P.caribaea by Hydroponics[J].Forestry Science and Technology of Guangdong Province,2012,28(6):20-26.
[21]Laubscher C P,Ndakidemi P A.Rooting success using IBA auxin on endangered Leucadendron laxum(proteaceae)in different rooting mediums[J].African Journal of Biotechnology,2008,7(19):3437-3442.
[22]Rauter R M.Rooting of Picea cuttings in plastic tubes[J].Canadian Journal of Forest Research,1971,1(2):125-129.
[2]Boc A A,Steward R B,Banko T J.Effects of sedum leaf cutting[J].Hort Science,1972,74(4):404-405.
[3]周心铁,杨承桂.针叶束育苗技术[M].北京:中国林业出版社,1985:6.Zhou X T,Yang C J.Seedling technique of needle fascicles[M].Beijing:China Forestry Publishing House,1985:6.
[4]于世河.我国针叶树水培技术研究进展[J].辽宁林业科技,2014,2(2):60-62.Yu S H.Research progress of conifer hydroponic technology in China[J].Forestry Science and Technology of Liaoning Province,2014,2(2):60-62.
[5]王以珊,韦战.马尾松湿地松针叶束育苗试验小结[J].广东林业科技,1988,1(1):18,29.Wang Y S,Wei Z.Needle fascicles seedling experiment summary of Pinus elliottii and Pinus massoniana[J].Forestry Science and Technology of Guangdong Province,1988,1(1):18,29
[6]杨承桂,周全国,赵文平等.湿地松无性系针叶束育苗生根的研究[J].湖北林业科技,1990,73(3):21-24,34.Yang C J,Zhou Q G,Zhao W P,et al.Rooting of needle fascicles of Pinus elliottii.[J].Forestry Science and Technology of Hubei Province,1990,73(3):21-24,34.
[7]唐德瑞,张成高.油松针叶束离体培养及植株重建研究[J].中国科技论文在线,2008,4(21):1-7.Tang D R,Zhang C G.Propagation of pinus tabulaeformis from needle fascicles[J].Science Paper Online of China,2008,4(21):1-7.
[8]李福秀,张晓明,周体林,等.思茅松针叶束水培生根的研究[J].西南林学院报,2005,25(1):25-27.Li F X,Zhang X M,Zhou T L,et al.Rooting experiment with needle aquiculture of Pinus kesiya var.langbianensis[J].Journal of Southwest Forestry College,2005,25(1):25-27.
[9]吴敏,宋西德,周锋利,等.柴松针叶束水培生根的研究[J].西北林学院学报,2009,24(2):60-62.Wu M,Song X D,Zhou F L,et al.Rooting of needle fascicles of Pinus tabulaeformis f.shekannesis by Water[J].Culture Journal of Northwest Forestry University,2009,24(2):60-62.
[10]陶锡岗,矫德胜.黑松针叶束育苗技术试验报告[J].山东林业科技,1985,55(2):28-31.Tao X G,Jiao D S.Needle fascicles seedling experiment summary of Pinus thunbergii[J].Journal of Shandong Forestry Science and Technology,1985,55(2):28-31.
[11]森下义郎.植物扦插理论与技术[M].李云森译.北京:中国林业出版社,1988:8.Morishita Yoshiro.Theory and technology of plant cutting[M].Li Y S translate.Beijing:China Forestry Publishing House,1988:8.
[12]汪天,许成林,何云核.黄刺玫插穗生根能力及其机理的探讨[J].安徽农业大学学报,2000,27(3):221-224.Wang T,Xu C L,He Y H.Studies on rooting capability and mechanism of cuttings of Rosa xanthine[J].Journal of Anhui Agricultural University,2000,27(3):221-224.
[13]车少辉.油松针叶束水培生根技术研究[D].杨凌:西北农林科技大学,2008.Che S H.Rooting of needle fascicles of Pinus tabulaeformis by water culture[D].Yangling:Northwest A&F University,2008.
[14]Bartel B,Le Clere S,Magidin M,et al.Inputs to the active indole-3-acetic acid pool:de novo synthesis,conjugate hydrolysis,and indole-3-butyric acidβ-oxidation[J].Journal of Plant Growth Regulation,2001,20(3):198-216.
[15]Nordstr m A C,Jacobs F A,Eliasson L.Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings[J].Plant Physiology,1991,96(3):856-861.
[16]Singh,K K,Gurung B.In vitro propagation of R.maddeni Hook.F.an endangered rhododendron species of Sikkim Himalaya[J].Notulae Botanicae Horti Agrobotanici Cluj-Napoca,2009,37(1):79-83.
[17]Chen X J,Zhou Y,Zhang Q X,et al.Cutting propagation of wild Rhododendron decorum Franch[J].Hort Science,2009,44(4):1104.
[18]Bashir M A,Anjum M A,Chaudhry Z,et al.Response of jojoba(Simmondsia chinensis)cuttings to various concentrations of auxins[J].Pak J Bot,2009,41(6):2831-2840.
[19]Rana R S,Sood K K.Effect of cutting diameter and hormonal application on the propagation of Ficus roxburghii wall.Through branch cuttings[J].Annals of Forest Research,2012,55(1):69-84.
[20]吴惠珊,张华通,赵奋成,等.湿加松针叶束水培生根研究[J].广东林业科技,2012,28(6):20-26.Wu H S,Zhang H T,Zhao F C,et al.Rooting of needle fascicles of Pinus elliottii×P.caribaea by Hydroponics[J].Forestry Science and Technology of Guangdong Province,2012,28(6):20-26.
[21]Laubscher C P,Ndakidemi P A.Rooting success using IBA auxin on endangered Leucadendron laxum(proteaceae)in different rooting mediums[J].African Journal of Biotechnology,2008,7(19):3437-3442.
[22]Rauter R M.Rooting of Picea cuttings in plastic tubes[J].Canadian Journal of Forest Research,1971,1(2):125-129.