好氧段碳源浓度对同步去除和富集磷酸盐生物膜的影响
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摘要
利用聚磷菌以循环交替O/A模式运行,对生活污水处理厂的主流工艺中实现磷酸盐的同步去除和富集,探究了好氧段碳源浓度对聚磷生物膜去除和富集磷酸盐性能以及生物膜中微生物种群结构的影响.结果表明,好氧COD质量浓度从200 mg·L~(-1)降低到0 mg·L~(-1),吸磷速率提升1. 29倍,出水磷质量浓度稳定在0. 5 mg·L~(-1)以下;释磷速率提升3. 56倍,富集液磷酸盐质量浓度从27. 125 mg·L~(-1)升高到55. 91 mg·L~(-1).微生物群落变化中,鉴定为聚磷菌的变形菌门(Proteobacteria)的含量增加约2倍,红环菌科(Rhodocyclaceae)和厌氧绳菌科(Anaerolineaceae)的富集效果分别提高了2. 28和5倍.降低好氧段碳源浓度,有利于聚磷菌的筛选和富集,强化了好氧段磷酸盐的去除以及厌氧段磷酸盐的释放,获得了更高的磷酸盐富集液,并且为以资源回收为目的的未来城市污水处理厂提供降低好氧段碳源需求的理论基础.
In a cyclic alternating O/A operation mode,phosphorus accumulating organisms( PAOs) can undertake phosphate removal and enrichment as the main process in wastewater treatment plants. The effects of the concentration of carbon sources during the aerobic stage on phosphate removal and enrichment performance of PAO biofilms,and the microbial population structure in the biofilms,were investigated. The results showed that the aerobic COD concentration decreased from 200 mg·L~(-1) to 0 mg·L~(-1),the phosphorus uptake rate improved by 1. 29 times,the phosphorus concentration in effluent stabilized below 0. 5 mg·L~(-1),the phosphorus release rate increased by 3. 56 times,and the phosphate concentration in the circulating solution increased from 27. 125 mg·L~(-1) to 55. 91 mg·L~(-1).With respect to the change in microbial communities,the identification showed that the abundance of Proteobacteria increased by approximately two times,and the enrichment effects of Rhodocyclaceae and Anaerolineaceae increased by 2. 28 and 5 times,respectively. Reducing the concentration of the carbon source in the aerobic section was beneficial to the screening and enrichment of PAOs,strengthening the removal of phosphate in the aerobic section and the release of phosphate in the anaerobic section. This resulted in an enriched phosphate solution. These observations provide a theoretical basis for future urban sewage treatment plants seeking to reduce their carbon demand.
In a cyclic alternating O/A operation mode,phosphorus accumulating organisms( PAOs) can undertake phosphate removal and enrichment as the main process in wastewater treatment plants. The effects of the concentration of carbon sources during the aerobic stage on phosphate removal and enrichment performance of PAO biofilms,and the microbial population structure in the biofilms,were investigated. The results showed that the aerobic COD concentration decreased from 200 mg·L~(-1) to 0 mg·L~(-1),the phosphorus uptake rate improved by 1. 29 times,the phosphorus concentration in effluent stabilized below 0. 5 mg·L~(-1),the phosphorus release rate increased by 3. 56 times,and the phosphate concentration in the circulating solution increased from 27. 125 mg·L~(-1) to 55. 91 mg·L~(-1).With respect to the change in microbial communities,the identification showed that the abundance of Proteobacteria increased by approximately two times,and the enrichment effects of Rhodocyclaceae and Anaerolineaceae increased by 2. 28 and 5 times,respectively. Reducing the concentration of the carbon source in the aerobic section was beneficial to the screening and enrichment of PAOs,strengthening the removal of phosphate in the aerobic section and the release of phosphate in the anaerobic section. This resulted in an enriched phosphate solution. These observations provide a theoretical basis for future urban sewage treatment plants seeking to reduce their carbon demand.
引文
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[23]夏雪,邵明非,吕小梅,等.不同碳源驯化除磷污泥的除磷效果及菌群结构分析[J].环境科学研究,2014,27(8):936-942.Xia X, Shao M F, Lyu X M, et al. Phosphorus removal performance and bacterial community structure of denitrifying phosphorus removal sludge operated with different carbon sources[J]. Research of Environmental Sciences,2014,27(8):936-942.
[24] Qiu G L,Zuniga-Montanez R Z,Law Y Y,et al. Polyphosphateaccumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources[J]. Water Research,2019,149:496-510.
[25]孟璇,潘杨,章豪,等.同步去除并富集磷酸盐生物膜驯化过程中微生物种群分析[J].环境科学,2018,39(6):2803-2809.Meng X,Pan Y,Zhang H,et al. Microbial population dynamics during domestication and cultivation of biofilm to remove and enrich phosphate[J]. Environmental Science,2018,39(6):2803-2809.
[26] Zilles J L,Hung C H,Noguera D R. Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal[J]. Water Science and Technology:A Journal of the International Association on Water Pollution Research,2002,46(1-2):123-128.
[27]孟璇,章豪,徐林建,等.同步去除并富集磷酸盐生物膜的驯化研究[J].现代化工,2018,38(10):85-90,92.Meng X,Zhang H,Xu L J,et al. Study on domestication and cultivation of biofilm to remove and enrich phosphates[J].Modern Chemical Industry,2018,38(10):85-90,92.
[2] Li W W,Yu H Q,Rittmann B E. Chemistry:Reuse water pollutants[J]. Nature,2015,528(7580):29-31.
[3] Barnard J L,Stevens G M,Leslie P J. Design strategies for nutrient removal plant[J]. Water Science and Technology,1985,17(11-12):233-242.
[4] Ali H I,Abd El-Azim M M,Abd El-Rahman M S,et al. The effects of modification for contact stabilization activated sludge on EBPR[J]. HBRC Journal,2015,11(1):143-149.
[5] Wong P Y,Cheng K Y,Bal Krishna K C,et al. Improvement of carbon usage for phosphorus recovery in EBPR-r and the shift in microbial community[J]. Journal of Environmental Management,2018,218:569-578.
[6]方柳静.生物膜处理城市污水脱氮除磷总结及展望[J].资源节约与环保,2018,(5):98.
[7]邹东雷,李平,杨卓悦,等.序批式移动床生物膜反应器强化反硝化除磷处理低碳源污水[J].科技导报,2016,34(2):237-240.Zou D L, Li P, Yang Z Y, et al. Enhanced denitrifying phosphorus removal to treat low carbon source sewage in sequencing batch moving bed biofilm reactor[J]. Science&Technology Review,2016,34(2):237-240.
[8] Kodera H,Hatamoto M,Abe K,et al. Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor[J]. Water Research,2013,47(6):2025-2032.
[9] Tian Q,Ong S K,Xie X H,et al. Enhanced phosphorus recovery and biofilm microbial community changes in an alternating anaerobic/aerobic biofilter[J]. Chemosphere,2016,144:1797-1806.
[10] Guisasola A,Pijuan M,Baeza J A,et al. Aerobic phosphorus release linked to acetate uptake in bio-P sludge:Process modeling using oxygen uptake rate[J]. Biotechnology and Bioengineering,2004,85(7):722-733.
[11]唐旭光,王淑莹,彭永臻,等. SBR法好氧段碳源浓度对吸磷的影响[J].中南大学学报(自然科学版),2011,42(10):3220-3224.Tang X G,Wang S Y,Peng Y Z,et al. Effect of carbon source on phosphorus uptake in aerobic condition[J]. Journal of Central South University(Science and Technology),2011,42(10):3220-3224.
[12]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002.
[13] Ruban V,López-Sánchez J F,Pardo P,et al. Development of a harmonised phosphorus extractionprocedure and certification of a sediment reference material[J]. Journal of Environmental Monitoring,2001,3(1):121-125.
[14]袁林江,周国标,南亚萍.微生物聚磷及其酶学调控[J].环境科学学报,2015,35(7):1955-1962.Yuan L J, Zhou G B, Nan Y P. Review on microbial polyphosphate accumulation and its enzymological regulation[J].Acta Scientiae Circumstantiae,2015,35(7):1955-1962.
[15] Wong P Y,Ginige M P,Kaksonen A H,et al. The ability of PAOs to conserve their storage-driven phosphorus uptake activities during prolonged aerobic starvation conditions[J].Journal of Water Process Engineering,2018,23:320-326.
[16] Majone M,Massanisso P,Ramadori R. Comparison of carbon storage under aerobic and anoxic conditions[J]. Water Science and Technology,1998,38(8-9):77-84.
[17] Jaffer Y,Clark T A,Pearce P,et al. Potential phosphorus recovery by struvite formation[J]. Water Research,2002,36(7):1834-1842.
[18]任皓甜,袁林江.磷酸盐浓度及pH对聚磷菌吸磷能力的影响[J].工业微生物,2018,48(4):17-23.Ren H T,Yuan L J. Effects of phosphate concentration and pH on phosphorus uptake by phosphorus-accumulating bacteria[J].Industrial Microbiology,2018,48(4):17-23.
[19]郑莹,潘杨,周晓华,等.一种新型生物膜法除磷工艺中聚磷菌的富集培养过程[J].环境科学,2017,38(1):277-282.Zheng Y,Pan Y,Zhou X H,et al. Process of enrichment and culture of PAOs on a novel biofilm process of dephosphorization[J]. Environmental Science,2017,38(1):277-282.
[20]张顺,田晴,汤曼琳,等.磷回收对厌氧/好氧交替式生物滤池蓄磷/除磷的影响[J].环境科学,2014,35(3):979-986.Zhang S,Tian Q,Tang M L,et al. Effect of phosphorus recovery on phosphorous bioaccumulation/harvesting in an alternating anaerobic/aerobic biofilter system[J]. Environmental Science,2014,35(3):979-986.
[21] Acevedo B,Murgui M,Borrás L,et al. New insights in the metabolic behaviour of PAO under negligible poly-P reserves[J].Chemical Engineering Journal,2017,311:82-90.
[22] Nguyen H TT,Le V Q,Hansen A A,et al. High diversity and abundance of putative polyphosphate-accumulating Tetrasphaerarelated bacteria in activated sludge systems[J]. FEMS Microbiology Ecology,2011,76(2):256-267.
[23]夏雪,邵明非,吕小梅,等.不同碳源驯化除磷污泥的除磷效果及菌群结构分析[J].环境科学研究,2014,27(8):936-942.Xia X, Shao M F, Lyu X M, et al. Phosphorus removal performance and bacterial community structure of denitrifying phosphorus removal sludge operated with different carbon sources[J]. Research of Environmental Sciences,2014,27(8):936-942.
[24] Qiu G L,Zuniga-Montanez R Z,Law Y Y,et al. Polyphosphateaccumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources[J]. Water Research,2019,149:496-510.
[25]孟璇,潘杨,章豪,等.同步去除并富集磷酸盐生物膜驯化过程中微生物种群分析[J].环境科学,2018,39(6):2803-2809.Meng X,Pan Y,Zhang H,et al. Microbial population dynamics during domestication and cultivation of biofilm to remove and enrich phosphate[J]. Environmental Science,2018,39(6):2803-2809.
[26] Zilles J L,Hung C H,Noguera D R. Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal[J]. Water Science and Technology:A Journal of the International Association on Water Pollution Research,2002,46(1-2):123-128.
[27]孟璇,章豪,徐林建,等.同步去除并富集磷酸盐生物膜的驯化研究[J].现代化工,2018,38(10):85-90,92.Meng X,Zhang H,Xu L J,et al. Study on domestication and cultivation of biofilm to remove and enrich phosphates[J].Modern Chemical Industry,2018,38(10):85-90,92.