基于镍丝负载氧化镍纳米片的尿酸生物传感器
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摘要
利用水热法与高温热处理相结合,在金属镍丝表面制备了氧化镍纳米片,这些纳米片相互交错,均匀覆盖于镍丝表面,其宽度约为数百纳米,厚度约10 nm.将镍丝负载氧化镍纳米片吸附固定尿酸氧化酶后,得到尿酸生物传感器电极,该电极显示出优异的电化学性能,其灵敏度达到821. 4μA/(mmol·cm~2),线性检测范围为1～900μmol/L,检出限为0. 1μmol/L,同时具有良好的抗干扰特性.该尿酸传感器电极易于进行植入式探测或与微流控技术相结合,为快速、高灵敏的尿酸检测提供了新途径.
Ni wire modified with Ni O nanosheets was synthesized by hydro-thermal method accompanied with heat treatment at high-temperature. The characterized results revealed that these Ni O nanosheets grew uniformly on the surface of the Ni wire,with a size of several hundreds of nanometers and a thickness of about ten nanometers. Electrochemical experiments showed that the Ni wire modified with Ni O nanosheets could be used as a kind of good material for immobilization of uricase to fabricate an efficient uric acid biosensor.Sensitivity of the prepared biosensor was found to be 821. 4 μA/( mmol · cm2). The linear range for the detection was 1—900 μmol/L. The detection limit was 0. 1 μmol/L. These results indicate that the Ni wire modified with Ni O nanosheets is a new platform for the construction of biosensors.
Ni wire modified with Ni O nanosheets was synthesized by hydro-thermal method accompanied with heat treatment at high-temperature. The characterized results revealed that these Ni O nanosheets grew uniformly on the surface of the Ni wire,with a size of several hundreds of nanometers and a thickness of about ten nanometers. Electrochemical experiments showed that the Ni wire modified with Ni O nanosheets could be used as a kind of good material for immobilization of uricase to fabricate an efficient uric acid biosensor.Sensitivity of the prepared biosensor was found to be 821. 4 μA/( mmol · cm2). The linear range for the detection was 1—900 μmol/L. The detection limit was 0. 1 μmol/L. These results indicate that the Ni wire modified with Ni O nanosheets is a new platform for the construction of biosensors.
引文
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[3] Ma Q. S.,Wang Q.,Zhao K. Z.,Zhai S. B.,Liu S.,Liu Z. Q.,Chem. J. Chinese Universities,2013,34(12),2716—2720(马青山,王茜,赵凯姝,翟淑波,刘舒,刘志强.高等学校化学学报,2013,34(12),2716—2720)
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[6] Liu B. H.,Deng J. Q.,Chem. J. Chinese Universities,1996,17(5),702—706(刘宝红,邓家祺.高等学校化学学报,1996,17(5),702—706)
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[8] Hou C.,Liu H.,Zhang D.,Yang C.,Zhang M.,J. Alloy. Compd.,2016,666,178—184
[9] KanJ. Q.,Mu S. L.,Acta Phys.-Chim. Sin.,1993,9(3),345—350(阚锦晴,穆绍林.物理化学学报,1993,9(3),345—350)
[10] Zhao J.,Mu F.,Qin L.,Jia X.,Yang C.,Mater. Chem. Phys.,2015,166,176—181
[11] Zhao J.,Qin L.,Hao Y.,Guo Q.,Mu F.,Yan Z.,Microchim. Acta,2012,178,439—445
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[14] Ahmad R.,Tripathy N.,Jang N. K.,Khang G.,Hahn Y.,Sensor. Actuat. B,2015,206,146—151
[15] Cheng C.,Kao C. Y.,Electroanal.,2016,28,695—703
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[17] Duan C.,Zhao J.,Qin L. Yang L.,Zhou Y.,Mater. Lett.,2017,208,65—68
[18] Chauhan N.,Pundir C. S.,Anal. Biochem.,2011,413,97—103
[19] Peng B.,Cui J.,Wang Y.,Liu J.,Zheng H.,Jin L.,Zhang X.,Zhang Y.,Wu Y.,Nanoscale,2018,10,1939—1945
[20] Arora K.,Tomar M.,Gupta V.,Analyst,2014,139,4606—4612
[21] Singh B.,Laffir F.,Dickinson C.,Mc Cormac T.,Dempsey E.,Electroanal.,2011,23(1),79—89
[22] Yang Y.,Jo A.,Lee Y.,Lee C.,Sens. Actuators B,2018,255,316—324
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