桃红侧耳子实体颜色的遗传规律研究
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摘要
子实体颜色是食用菌的重要商品性状之一,也是食用菌遗传育种的重要方向。以桃红侧耳[Pleurotus djamor(Rumph. ex Fr.)Boedijn]的粉红色菌株CCMSSC00450和灰褐色菌株CCMSSC04445为亲本,通过原生质体单核化技术,分别获得各自的组成型单核菌株PDm52、PDm17和PDm99、PDm14。经相互配对杂交和出菇试验,4个杂交子均可结实,且色泽不同,其中PDm52×PDm99是粉红色、PDm52×PDm14是粉灰色、PDm17×PDm14是灰褐色、PDm17×PDm99是白色。以粉红色杂交子PDm52×PDm99的207个单孢单核体作为粉红色子实体的分离群体,以PDm17为测交菌株,构建测交群体。出菇结果显示,测交群体的子实体颜色出现明显的性状分离,由深到浅连续分布,存在粉红色、淡粉色、白色等。结果表明,P. djamor子实体的粉红色相对于白色为不完全显性,白色为隐性;子实体颜色性状是具有高遗传力的多基因控制的数量性状;菌盖颜色和菌褶颜色存在极显著相关性。
The color of fruiting body is one of the most important commercial traits of edible mushrooms and an important objective for breeding superior mushroom cultivars. In this study,a pink strain CCMSSC00450 and a taupe strain CCMSSC04445 of Pleurotus djamor(Rumph. ex Fr.)Boedijn were used as dikaryotic parental lines,and the constitutive monokaryons of two strains i.e.,PDm52 and PDm17 for strain CCMSSC00450,PDm99 and PDm14 for CCMSSC04445,were obtained by protoplast monokaryonization. After the cross-mating of4 constitutive monokaryons,4 hybrid dikaryons were generated and cultivated for phenotyping. The 4 hybrid strains showed different color,i.e.,pink for PDm52×PDm99,pinkish gray for PDm52×PDm14,taupe for PDm17×PDm14,and white for PDm17×PDm99. A total 207 single spore isolates of the pink hybrid(PDm52×PDm99)were randomly selected as the segregating population of the color of pink fruiting body and PDm17 as the tester line,the test-cross population was constructed. Fruiting results showed that the color of the test-cross population was obviously segregated in a continuous distribution pattern varying from dark to light with pink,light pink,white,etc. The results revealed that the pink of the fruiting body was not completely dominant with respect to the white,and the white was recessive. Color of fruiting body of P.djamor is a quantitative trait controlled by multiple genes with high heritability. Meanwhile,a significant correlation was detected between the colors of the cap and gill.
The color of fruiting body is one of the most important commercial traits of edible mushrooms and an important objective for breeding superior mushroom cultivars. In this study,a pink strain CCMSSC00450 and a taupe strain CCMSSC04445 of Pleurotus djamor(Rumph. ex Fr.)Boedijn were used as dikaryotic parental lines,and the constitutive monokaryons of two strains i.e.,PDm52 and PDm17 for strain CCMSSC00450,PDm99 and PDm14 for CCMSSC04445,were obtained by protoplast monokaryonization. After the cross-mating of4 constitutive monokaryons,4 hybrid dikaryons were generated and cultivated for phenotyping. The 4 hybrid strains showed different color,i.e.,pink for PDm52×PDm99,pinkish gray for PDm52×PDm14,taupe for PDm17×PDm14,and white for PDm17×PDm99. A total 207 single spore isolates of the pink hybrid(PDm52×PDm99)were randomly selected as the segregating population of the color of pink fruiting body and PDm17 as the tester line,the test-cross population was constructed. Fruiting results showed that the color of the test-cross population was obviously segregated in a continuous distribution pattern varying from dark to light with pink,light pink,white,etc. The results revealed that the pink of the fruiting body was not completely dominant with respect to the white,and the white was recessive. Color of fruiting body of P.djamor is a quantitative trait controlled by multiple genes with high heritability. Meanwhile,a significant correlation was detected between the colors of the cap and gill.
引文
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[22]潘迎捷,陈明杰,汪昭月,等.香菇菌丝原生质体的分离与培养[J].上海农业学报, 1989, 5(1):25-30.
[23]黄毅.食用菌栽培[M].北京:高等教育出版社, 2008.
[24]Weijn A, Tomassen MMM, Bastiaannet S, et al. A new method to apply and quantify bruising sensitivity of button mushrooms[J].LWT-Food Science and Technology, 2012, 47(2):308-314.
[25]Gao W, Weijn A, Baars JJP, et al. Quantitative trait locus mapping for bruising sensitivity and cap color of Agaricus bisporus(button mushrooms)[J]. Fungal Genetics&Biology, 2015, 77:69-81.
[26]Muraguchi H, Umezawa K, Niikura M, et al. Strand-Specific RNA-Seq analyses of fruiting body development in Coprinopsis cinerea[J]. PLoS One, 2015, 10(10):e0141586.
[27]Nakazawa T, Ando Y, Kitaaki K, et al. Efficient gene targeting inΔCc. ku70 orΔCc. lig4 mutants of the agaricomycete Coprinopsis cinerea[J]. Fungal Genetics&Biology, 2011, 48(10):939-946.
[28]Nakazawa T, Ando Y, Kitaaki K, et al. A mutation in the Cc. ubc2gene affects clamp cell morphogenesis as well as nuclear migration for dikaryosis in Coprinopsis cinerea[J]. Fungal Genetics&Biology, 2011, 48(5):519-525.
[29]Ohm RA, Jong JFD, Berends E, et al. An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune[J]. World Journal of Microbiology and Biotechnology,2010, 26(10):1919-1923.
[30]Jong JFD, Ohm RA, Bekker CD, et al. Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination[J]. FEMS Microbiol Lett, 2010, 310(1):91-95.
[2]Jiao F, Wang X, Song X, et al. Processing optimization and antioxidative activity of enzymatic extractable polysaccharides from Pleurotus djamor[J]. International Journal of Biological Macromolecules, 2017, 98:469-478.
[3]张其昌,黄谚谚,赖万年,等.红平菇RO-1营养成分分析[J].食用菌, 1995(4):12.
[4]Rampinelli JR, Silveira MLL, Gern RMM, et al. Nutritional value of Pleurotus djamor cultivated in banana straw[J]. Alimentos E Nutri??o, 2010, 21(2):197-202.
[5]Foulongne-Oriol M, Rodier A, Rousseau T, et al. Quantitative geneticstodissectthefungal-fungalinteractionbetween Lecanicillium verticillium and the white button mushroom Agaricus bisporus[J]. Fungal Biology, 2011, 115(4-5):421-431.
[6]Ramírez L, Larraya LM, Pisabarro AG. Molecular tools for breeding basidiomycetes[J]. International Microbiology:the Official Journal of the Spanish Society for Microbiology, 2000, 3(3):147.
[7]Salzberg AM. Breeding edible mushrooms:State of the art[J].Journal of Pediatric Surgery, 2005, 6(6):793-793.
[8]廖剑华,陈美元,卢政辉,等.双孢蘑菇子实体颜色的遗传规律研究[J].菌物学报, 2007, 26(增刊):138-140.
[9]Callac P, Moquet F, Imbernon M, et al. Evidence for PPC1,a determinant of the pilei-pellis color of Agaricus bisporus fruitbodies[J]. Fungal Genetics&Biology, 1998, 23(2):181.
[10]谢宝贵,江玉姬,吴文礼.金针菇子实体颜色的遗传规律研究[J].菌物学报, 2004, 23(1):79-84.
[11]盛春鸽,黄晨阳,陈强.白黄侧耳子实体颜色遗传规律[J].中国农业科学, 2012, 45(15):3124-3129.
[12]周伟峰.红平菇的人工栽培[J].中国食用菌, 1994(6):24-25.
[13]熊芳,朱坚,邓优锦,等.红平菇(Pleurotus diamor)培育条件和栽培技术研究[J].江西农业大学学报, 2011, 33(5):1006-1011.
[14]池玉杰,伊洪伟,刘智会.红平菇菌株H1的培养特性与营养成分分析[J].东北林业大学学报, 2007, 35(1):53-57.
[15]张建军.红平菇菌丝体多糖的提取、结构分析和抗糖尿病作用研究[D].泰安:山东农业大学, 2016.
[16]Zhang J, Liu M, Yang Y, et al. Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor[J]. Carbohydrate Polymers, 2016, 136(6):588-597.
[17]Oliveira, APD, Naozuka J. Preliminary results on the feasibility of producing selenium-enriched pink(Pleurotus djamor)and white(Pleurotus ostreatus)oyster mushrooms:bioaccumulation,bioaccessibility, and Se-proteins distribution[J]. Microchemical Journal, 2019, 145:1143-1150.
[18]郑苗苗,邵淑丽,张令昂,等.红平菇HP1漆酶基因及启动子克隆和序列分析[J].江苏农业科学, 2014, 42(8):21-26.
[19]郑苗苗,邵淑丽,张东向,等.红平菇漆酶基因异源表达及对染料脱色的研究[J].纺织学报, 2014, 35(12):84-90.
[20]Wesenberg D, Kyriakides I, Agathos SN. White-rot fungi and their enzymes for the treatment of industrial dye effluents[J].Biotechnology Advances, 2003, 22(1-2):161-187.
[21]Yildirim N, Tanyol M, Dere T, et al. The investigation on physicochemical parameters of the textile effluents after treatment by white rot fungus Pleurotus djamor[J]. New Biotechnology, 2012, 29:S184-S184.
[22]潘迎捷,陈明杰,汪昭月,等.香菇菌丝原生质体的分离与培养[J].上海农业学报, 1989, 5(1):25-30.
[23]黄毅.食用菌栽培[M].北京:高等教育出版社, 2008.
[24]Weijn A, Tomassen MMM, Bastiaannet S, et al. A new method to apply and quantify bruising sensitivity of button mushrooms[J].LWT-Food Science and Technology, 2012, 47(2):308-314.
[25]Gao W, Weijn A, Baars JJP, et al. Quantitative trait locus mapping for bruising sensitivity and cap color of Agaricus bisporus(button mushrooms)[J]. Fungal Genetics&Biology, 2015, 77:69-81.
[26]Muraguchi H, Umezawa K, Niikura M, et al. Strand-Specific RNA-Seq analyses of fruiting body development in Coprinopsis cinerea[J]. PLoS One, 2015, 10(10):e0141586.
[27]Nakazawa T, Ando Y, Kitaaki K, et al. Efficient gene targeting inΔCc. ku70 orΔCc. lig4 mutants of the agaricomycete Coprinopsis cinerea[J]. Fungal Genetics&Biology, 2011, 48(10):939-946.
[28]Nakazawa T, Ando Y, Kitaaki K, et al. A mutation in the Cc. ubc2gene affects clamp cell morphogenesis as well as nuclear migration for dikaryosis in Coprinopsis cinerea[J]. Fungal Genetics&Biology, 2011, 48(5):519-525.
[29]Ohm RA, Jong JFD, Berends E, et al. An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune[J]. World Journal of Microbiology and Biotechnology,2010, 26(10):1919-1923.
[30]Jong JFD, Ohm RA, Bekker CD, et al. Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination[J]. FEMS Microbiol Lett, 2010, 310(1):91-95.
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