氯霉素单分散限进分子印迹聚合物的制备及其识别特性研究
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摘要
以自制的单分散交联聚甲基丙烯酸环氧丙酯(P_(GMA/EDMA))为固相基质,氯霉素(CAP)为模板分子,采用表面原子转移自由基聚合技术(SI-ATRP)制备氯霉素单分散限进分子印迹聚合物(CAP-MRAM-MIPs)。采用傅里叶变换红外光谱(FT-IR)、扫描电镜(SEM)、热重(TGA)、元素分析和接触角等方法对其表征,比表面积、孔体积和孔径的结果表明,与氯霉素单分散限进非印迹聚合物(CAP-MRAM-NIPs)相比,CAP-MRAM-MIPs具有更大的孔径和孔体积。CAP-MRAM-MIPs对牛血清白蛋白(BSA)的排阻能力为97.2%;对CAP的最大吸附量为56.7 mg/g,分配系数(K_d)为34.42 mL/g,印迹因子(α)为2.25。将CAP-MRAM-MIPs用于分离富集牛奶中的CAP,应用高效液相色谱(HPLC)检测,加标回收率为93.5%～102.6%。
Chloramphenicol monodispersed restricted access media-molecularly imprinted polymers (CAPMRAM-MIPs) were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) with monodisperse crosslinked poly (Glycidyl methacrylate-co-ethylenedimethacrlate) (P_(GMA/EDMA)) microspheres as solid phase matrix, and chloramphenicol (CAP) as template molecules. The imprinted polymers were characterized by Fourier transform infrared (FT-IR) spectrum, scanning electron microscope (SEM),thermogravimetric (TGA),element analysis and contact angle method. Furthermore,the results of specific surface area,pore volume and pore size showed that the pore size and pore volume of CAP-MRAM-MIPs were larger than those of the chloramphenicol monodisperse restricted access media-molecularly non-imprinted polymers (CAP-MRAM-NIPs). The exclusion of CAP-MRAM-MIPs to bovine serum albumin (BSA) was97.2%. The maximum absorbed amounts,distribution coefficient (K_d) and imprinting factors(α) of CAP were 56.7 mg/g,34.42 mL/g and 2.25 mg/g. By combining with high performance liquid chromatographic(HPLC) analysis,the CAP-MRAM-MIPs were applied to the separation and enrichment of CAP in milk samples with recoveries of 93.5%-102.6%.
Chloramphenicol monodispersed restricted access media-molecularly imprinted polymers (CAPMRAM-MIPs) were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) with monodisperse crosslinked poly (Glycidyl methacrylate-co-ethylenedimethacrlate) (P_(GMA/EDMA)) microspheres as solid phase matrix, and chloramphenicol (CAP) as template molecules. The imprinted polymers were characterized by Fourier transform infrared (FT-IR) spectrum, scanning electron microscope (SEM),thermogravimetric (TGA),element analysis and contact angle method. Furthermore,the results of specific surface area,pore volume and pore size showed that the pore size and pore volume of CAP-MRAM-MIPs were larger than those of the chloramphenicol monodisperse restricted access media-molecularly non-imprinted polymers (CAP-MRAM-NIPs). The exclusion of CAP-MRAM-MIPs to bovine serum albumin (BSA) was97.2%. The maximum absorbed amounts,distribution coefficient (K_d) and imprinting factors(α) of CAP were 56.7 mg/g,34.42 mL/g and 2.25 mg/g. By combining with high performance liquid chromatographic(HPLC) analysis,the CAP-MRAM-MIPs were applied to the separation and enrichment of CAP in milk samples with recoveries of 93.5%-102.6%.
引文
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29 Wang Y,Wang Y X,Chen L,Wan Q H.J.Magn.Magn.Mater.,2012,324(4):410-417
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2 Lu Y,Yao H,Li C,Han J,Tan Z J,Yan Y S.Food Chem.,2016,192(2):163-170
3 DING Qiao-Qi,LI Li,FAN Wen-Tao,LYV Ya-Nan,HU Jian-Hua,YAN Li-Ping,SONG Su-Quan.Chinese J.Anal.Chem.,2017,45(11):1686-1693丁乔棋,李丽,范文韬,吕亚楠,胡建华,闫丽萍,宋素泉.分析化学,2017,45(11):1686-1693
4 Li Z Y,Quan H J,Gong C B,Yang Y Z,Tang Q,Wei Y B,Ma X B.Food Chem.,2015,172:56-62
5 Johnston L,Mackay L,Croft M.J.Chromatogr.A,2002,982(1):97-109
6 Kaufmann A,Butcher P,Maden K,Walker S,Widmer M.Anal.Chim.Acta,2014,820:56-68
7 Crescenzi C,Bayoudh S,Cormack P A G,Klein T,Ensing K.Anal.Chem.,2001,73(10):2171-2177
8 Shi Z H,Zhang S L,Huai Q R,Xu D,Zhang H Y.Talanta,2017,162:300-308
9 CAI Ya-Qi,MOU Shi-Fen.Chinese J.Anal.Chem.,2005,33(11):1647-1652蔡亚岐,牟世芬.分析化学,2005,33(11):1647-1652
10 He J,Song L,Chen S,Li Y Y,Wei H L,Zhao D X,Gu K R,Zhang S S.Food Chem.,2015,187:331-337
11 ZHANG Yuan,LI Long,SU Li-Qiang,CHU Hong-Tao,SUN Lin,TAN Xiao,DU Yi-Ping.Chinese J.Anal.Chem.,2018,46(5):729-734张园,李龙,苏立强,初洪涛,孙琳,覃潇,杜一平.分析化学,2018,46(5):729-734
12 TONG Yu-Kui,HU Yue,XIA Qin-Fei,HUANG Wei,TIAN Miao-Miao.Chinese Journal of Chromatography,2017,35(3):291-301佟育奎,胡月,夏琴飞,黄玮,田苗苗.色谱,2017,35(3):291-301
13 DENG Hui-Yun,WANG Bin,WU Mao,WEN Rui-Zhi,MA Qiang,GUO Ya-Ping,DENG Bin,XIE Lian-Wu.Chinese J.Appl.Chem.,2018,35(5):600-608邓慧芸,王斌,吴茂,文瑞芝,马强,郭亚平,邓斌,谢练武.应用化学,2018,35(5):600-608
14 Martín-Esteban A.Tr AC-Trend.Anal.Chem.,2013,45(4):169-181
15 ZHAO Li-Juan,QI Yu-Xia,WEI Chan-Ling,LI Wen-Jing,LI Ya,WANG Fu-Qiang,GONG Bo-Lin.Chinese J.Anal.Chem.,2016,44(10):1562-1567赵丽娟,祁玉霞,魏缠玲,李文婧,李亚,王富强,龚波林.分析化学,2016,44(10):1562-1567
16 Xiao D,Dramou P,Xiong N Q,He H,Yuan D,Dai H,Li H,He X,Peng J,Li N.Analyst,2013,138(11):3287-3296
17 LIAO Su-Lan,CHEN Shao-Yun,LIU Qi-Lin,CHEN Liang-Bi,LI Xiao-Min.Chinese J.Anal.Chem.,2018,46(1):100-106廖素兰,陈少云,刘奇琳,陈良壁,李晓敏.分析化学,2018,46(1):100-106
18 YANG Xu,LIU Mei-Jiao,LIN Shen,XU Dan,DONG Xiang-Chao.Chinese J.Anal.Chem.,2016,44(1):146-151杨旭,刘美娇,林深,徐丹,董襄朝.分析化学,2016,44(1):146-151
19 Vielhauer S,Rudolphi A,Boos K S,Seidel D.J.Chromatogr.B,1995,666(2):315-322
20 Xu W J,Su S F,Jiang P,Wang H S,Dong X C,Zhang M.J.Chromatogr.A,2010,1217(46):7198-7207
21 Wang C Z,Li M,Xu H H,Wei Y M.J.Chromatogr.A,2014,1343:195-199
22 Liu L J,Yang B C,Zhang F F,Liang X M.Anal.Methods,2017,9(20):2990-2996
23 Du B,Qu T T,Chen Z,Cao X H,Han S P,Shen G P,Wang L.Talanta,2014,129:465-472
24 Pattend T E,Matyjaszewskishi K.Adv.Mater.,1998,10(12):901-915
25 Wang Y,Zhang X,Yan J L,Yan X,Lang M D.Appl.Surf.Sci.,2011,257(14):6233-6238
26 Liu Y L,Huang Y Y,Liu J Z,Wang W Z,Liu G Q,Zhao R.J.Chromatogr.A,2012,1246:15-21
27 Gong B L,Ke C Y,Geng X D.Anal.Bioanal.Chem.,2003,375(6):769-774
28 Niu Y L,Liu C A,Yang J,Ma M H,Gong Y R,Wang Y,Gong B L.Food Anal.Methods,2016,9(8):2342-2351
29 Wang Y,Wang Y X,Chen L,Wan Q H.J.Magn.Magn.Mater.,2012,324(4):410-417
30 Francesco P,Francesca I,Giuseppe C,Manuela C,Ortensia I P,Umile G S,Nevio Picci.Eur.Polym.J.,2009,45:1634-1640
31 Samanidou V,Kehagia M,Kabir A,Furton K G.Anal.Chim.Acta,2016,914:62-74