ICAM-1和8-OHdG在肾癌组织中的表达及其与临床病理指标的关系
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摘要
目的:观察ICAM-1和8-OHdG在肾癌组织中的表达情况,并分析两者的表达与肾癌患者临床病理参数之间的关系,探讨其在肾癌发生和进展中的作用。方法:采用免疫组化的方法检测46例肾癌组织和18例远离癌组织的正常肾组织中ICAM-1和8-OHdG的表达,通过阳性染色强度及阳性细胞百分比进行综合评分,观察不同组织者中两者的表达差异,同时分别统计分析ICAM-1和8-OHdG的表达与肾癌患者年龄、性别、肿瘤大小、肿瘤分期以及是否有淋巴结转移或远处转移等临床病理参数之间的关系。结果:ICAM-1在正常肾组织中的表达多为弱阳性或阴性表达,而在肾癌组织中ICAM-1阳性表达增加(占比82. 6%),且与肿瘤的分化程度、淋巴结转移和远处转移具有显著相关性(P<0. 05或P<0. 01)。绝大多数正常肾组织中8-OHdG呈阴性表达,而肾癌组织中8-OHdG阳性表达增加(占比63%),与肿瘤大小、淋巴结转移和远处转移以及无进展生存期(PFS)之间具有显著相关性(P<0. 05或P<0. 01)。结论:ICAM-1和8-OHdG在肾癌组织中高表达,二者在肾癌的发生和进展中可能发挥着重要的作用。
Objective: To observe the expression of ICAM-1 and 8-OHdG in renal cell carcinoma,and to analyze the relationship between the expression of ICAM-1 and 8-OHdG and clinicopathological parameters of renal cell carcinoma patients,and to explore their roles in the occurrence and progression of renal cell carcinoma. Methods: The expression of ICAM-1 and 8-OHdG in 46 renal cancer tissues and 18 normal renal tissues far from cancer tissues were detected by immunohistochemical method. The expression of ICAM-1 and 8-OHdG in different tissues was observed by comprehensive score of positive staining intensity and percentage of positive cells. The expression of ICAM-1 and 8-OHdG in different tissues of renal cancer patients was observed. The relationship was statistically analyzed between the expression of ICAM-1 and 8-OHdG and clinicopathological parameters such as age,sex,tumor size and stage,lymph node metastasis or distant metastasis. Results: The expression of ICAM-1 in normal kidney tissues was weak positive or negative,but increased in renal cancer tissues( 82. 6%). ICAM-1 expression was significantly correlated with the degree of differentiation,lymph node metastasis and distant metastasis( P<0. 05 or P<0. 01). The expression of 8-OHdG was negative in most normal kidney tissues,but increased in renal cancer tissues( 63%). There was a significant correlation between 8-OHdG expression and tumor size,lymph node metastasis,distant metastasis and progression-free survival( PFS)( P<0. 05 or P<0. 01). Conclusion: ICAM-1 and 8-OHdG were highly expressed in renal cell carcinoma,which may play an important role in the occurrence and progression of renal cell carcinoma.
Objective: To observe the expression of ICAM-1 and 8-OHdG in renal cell carcinoma,and to analyze the relationship between the expression of ICAM-1 and 8-OHdG and clinicopathological parameters of renal cell carcinoma patients,and to explore their roles in the occurrence and progression of renal cell carcinoma. Methods: The expression of ICAM-1 and 8-OHdG in 46 renal cancer tissues and 18 normal renal tissues far from cancer tissues were detected by immunohistochemical method. The expression of ICAM-1 and 8-OHdG in different tissues was observed by comprehensive score of positive staining intensity and percentage of positive cells. The expression of ICAM-1 and 8-OHdG in different tissues of renal cancer patients was observed. The relationship was statistically analyzed between the expression of ICAM-1 and 8-OHdG and clinicopathological parameters such as age,sex,tumor size and stage,lymph node metastasis or distant metastasis. Results: The expression of ICAM-1 in normal kidney tissues was weak positive or negative,but increased in renal cancer tissues( 82. 6%). ICAM-1 expression was significantly correlated with the degree of differentiation,lymph node metastasis and distant metastasis( P<0. 05 or P<0. 01). The expression of 8-OHdG was negative in most normal kidney tissues,but increased in renal cancer tissues( 63%). There was a significant correlation between 8-OHdG expression and tumor size,lymph node metastasis,distant metastasis and progression-free survival( PFS)( P<0. 05 or P<0. 01). Conclusion: ICAM-1 and 8-OHdG were highly expressed in renal cell carcinoma,which may play an important role in the occurrence and progression of renal cell carcinoma.
引文
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[18]杨延辉,张玉祥,桂洋,等.胰腺癌与细胞自噬、神经浸润的关系[J].临床肝胆病杂志,2018,34(11):2475-2479.Yang YH,Zhang YX,Gui Y,et al.Research advances in the role of antophagy and perieural invasion in pancreatic cancar[J].JClin Hepatol,2018,34(11):2475-2479.
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[2]Stühler V,Bedke J.Overview of treatment of localized and metastatic renal cell carcinoma(RCC)[J].MMW Fortschr Med,2018,160(8):45-51.
[3]Lee HJ,Pyo MC,Shin HS,et al.Renal toxicity through AhR,PXR,and Nrf2 signaling pathway activation of ochratoxin A-induced oxidative stress in kidney cells[J].Food Chem Toxicol,2018,122:59-68.
[4]Zhu J,Lian L,Qin H,et al.Prognostic evaluation of patients with resectable lung cancer using systemic inflammatory response parameters[J].Oncol Lett,2019,17(2):2244-2256.
[5]Lafaro KJ.Melstrom LG.The paradoxical web of pancreatic cancer tumor microenvironment[J].Am J Pathol,2019,189(1):44-57.
[6]Zhang P,Goodrich C,Fu C,et al.Melanoma upregulates ICAM-1expression on endothelial cells through engagement of tumor CD44with endothelial E-selectin and activation of a PKCα-p38-SP-1pathway[J].FASEB J,2014,28(11):4591-4609.
[7]Bonan S,Albrengues J,Grasset E,et al.Membrane-bound ICAM-1contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts[J].Oncotarget,2017,8(1):1304-1320.
[8]魏玉亮,樊方平,汤志亮,等.ICAM-1和STAT4在肝细胞癌中的表达及其与侵袭转移的关系[J].江西医药,2017,52(6):571-573.Wei YL,Fan FP,Tang ZL,et al.ICAM-1 and STAT4 expression in hepatocellular carcinoma and their relationship with invasion and metastasis[J].Jiangxi Med,2017,52(6):571-573.
[9]陈晓燕,李庆姝.淋巴管生成与细胞间粘附分子-1在宫颈癌中表达的临床意义[J].重庆医科大学学报,2018,43(2):250-254.Chen XY,Li QS.Clinical significance of lymphangiogenesis and expression of intercellular adhesion molecule-1 in cervical cancer[J].J Chongqing Med Univ,2018,43(2):250-254.
[10]Luo L,Xia L,Zha BS,et al.miR-335-5p targeting ICAM-1inhibits invasion and metastasis of thyroid cancer cells[J].Biomed Pharmacotherapy,2018,106:983-990.
[11]主余华,傅丽娜,孙成刚,等.细胞间粘附分子-1在原发性肝癌的检测及意义[J].临床肝胆病杂志,2002,18(3):154-156.Zhu YH,Fu L,Sun CG,et al.Detection and significance of intercellular adhesion molecule-1 in primary hepatocellular carcinoma[J].J Clin Hepatobiliary Disease,2002,18(3):154-156.(下转第页)
[12]Reina M,Espel E.Role of LFA-1 and ICAM-1 in cancer[J].Cancers(Basel),2017,9(11):E153.
[13]Yanguas A,Garasa S,Teijeira,et al.ICAM-1-LFA-1 dependent CD8+T-lymphocyte aggregation in tumor tissue prevents recirculation to draining lymph nodes[J].Front Immunol,2018,9:2084.
[14]罗思佳,梅洪亮,叶家欣,等.腹腔镜肝癌切除术对肝细胞癌中的治疗效果及对血清AFP、s EC、HGF、ICAM-1的影响[J].现代生物医学进展,2018,18(2):301-304.Luo SJ,Mei HL,Ye JX,et al.The therapeutic effect of laparoscopic hepatectomy on hepatocellular carcinoma and its influence on serum AFP,s EC,HGF and ICAM-1[J].Adv Modern Biomed,2018,18(02):301-304.
[15]廖维甲,梅铭惠,覃理灵,等.肝细胞癌血清血管内皮生长因子和细胞间粘附分子-1的测定及其临床意义[J].右江医学,2006,(2):126-127.Liao WJ,Mei MH,Tan LL,et al.Determination of serum vascular endothelial growth factor and intercellular adhesion molecule-1 in hepatocellular carcinoma and their clinical significance[J].Youjiang Med Sci,2006,34(2):126-127.
[16]Schumacker PT.Reactive oxygen species in cancer:a dance with the devil[J].Cancer Cell,2015,27(2):156-157.
[17]Singh AK,Awasthi D,Dubey M,et al.High oxidative stress adversely affects nfkappab mediated induction of inducible nitric oxide synthase in human neutrophils:Implications in chronic myeloid leukemia[J].Nitric Oxide,2016,58:28-41.
[18]杨延辉,张玉祥,桂洋,等.胰腺癌与细胞自噬、神经浸润的关系[J].临床肝胆病杂志,2018,34(11):2475-2479.Yang YH,Zhang YX,Gui Y,et al.Research advances in the role of antophagy and perieural invasion in pancreatic cancar[J].JClin Hepatol,2018,34(11):2475-2479.
[19]Saeed U,Piracha ZZ,Manzoor S.Hepatitis C virus induces oxidative stress and DNA damage by regulating DNAPKCs,ATM,ATRand PARP mediated signaling and guards cell from cancerous condition by upregulating RB,P53 and downregulating VEGF[J].Acta Virol,2017,61(3):316-323.
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