不同扁穗雀麦种质苗期抗旱性鉴定与评价
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摘要
为比较21份不同扁穗雀麦(Bromus catharticus)种质资源的苗期抗旱性,测定了在20%浓度的PEG-6000模拟干旱胁迫下扁穗雀麦苗期的5个生理生化指标:相对含水量(RWC)、相对电导率(REC)、丙二醛含量(MDA)、过氧化物酶活性(POD)和游离脯氨酸含量(Fpro)。结果表明,供试21份扁穗雀麦种质经模拟干旱胁迫前后,各项生理生化指标均发生明显变化,不同种质间总体上存在显著差异(P<0.05)。根据供试种质的抗旱性隶属函数度量值(D),可将其分为4类不同的抗旱型,其中Ⅰ类4份,Ⅱ类5份,Ⅲ类3份,Ⅳ类9份,且4类供试种质的抗旱性强弱表现为Ⅰ>Ⅱ>Ⅲ>Ⅳ。在21份扁穗雀麦供试种质中,以材料PI442076的抗旱性最强,材料PI292258的抗旱性最弱。
The study was conducted to estimate the drought tolerance of 21 Bromus catharticus germplasms by exposing them to 20% PEG-6000;five physiological and biochemical indices:relative water content,relative electric conductivity,malondialdehyde content,free proline content,and peroxidase activity were measured.The results showed that the changes in physiological and biochemical indices of the 21 B.catharticus germplasms were apparent after exposure to the simulated stress,and their values were significantly different.According to their subordinate function value(D),these germplasms can be divided into 4 different types:4 germplasms belonged to classⅠ,5 to classⅡ,4 to classⅢ,and 9 to classⅣ.The drought tolerance of these4 types of germplasms in descending order isⅠ>Ⅱ>Ⅲ>Ⅳ.Among the 21 different B.catharticus germplasms,PI442076 showed the strongest drought resistance while PI292258 showed the weakest.
The study was conducted to estimate the drought tolerance of 21 Bromus catharticus germplasms by exposing them to 20% PEG-6000;five physiological and biochemical indices:relative water content,relative electric conductivity,malondialdehyde content,free proline content,and peroxidase activity were measured.The results showed that the changes in physiological and biochemical indices of the 21 B.catharticus germplasms were apparent after exposure to the simulated stress,and their values were significantly different.According to their subordinate function value(D),these germplasms can be divided into 4 different types:4 germplasms belonged to classⅠ,5 to classⅡ,4 to classⅢ,and 9 to classⅣ.The drought tolerance of these4 types of germplasms in descending order isⅠ>Ⅱ>Ⅲ>Ⅳ.Among the 21 different B.catharticus germplasms,PI442076 showed the strongest drought resistance while PI292258 showed the weakest.
引文
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[6] MWADZINGENI L,SHIMELIS H,DUBE E.Breeding wheat for drought tolerance:Progress and technologies.Journal of Integrative Agriculture,2016,15(5):935-943.
[7] CHEN X,MIN D H,YASIR TA,HU Y G.Evaluation of 14morphological,yield-related and physiological traits as indicators of drought tolerance in Chinese winter bread wheat revealed by analysis of the membership function value of drought tolerance(MFVD).Field Crops Research,2012,137:195-201.
[8]郭郁频,米福贵,闫利军,任永霞,吕世杰,伏兵哲.不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价.草业学报,2014(4):220-228.GUO Y P,MI F G,YAN L J,REN Y X,LYU S J,FU B Z.Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties.Acta Prataculturae Sinica,2014(4):220-228.
[9]曹俊梅,芦静,周安定,刘联正,张新忠,黄天荣,高永红,吴新元.水分胁迫对新疆不同小麦品种幼苗生理特性的影响.新疆农业科学,2014(7):1190-1196.CAO J M,LU J,ZHOU A D,LIU L Z,ZHANG X Z,HUANG T R,GAO Y H,WU X Y.Effects of water stress on the physiological action of different Xinjiang winter wheat varieties in seedling.Xinjiang Agricultural Sciences,2014(7):1190-1196.
[10]刘新,符开欣,郭志慧,张新全,白史且,李达旭,张昌兵,张成林,孙铭,马啸.6份川西北短芒披碱草种质苗期的抗旱性综合评价.草业科学,2017,34(1):101-111.LIU X,FU K X,CUO Z H,ZHANG X Q,BAI S Q,Li D X,ZHANG C B,ZHANG C L,SUN M,MA X.Comprehensive evaluation of drought resistancc in six Elymus breviaristatus accessions from the Northwest Plateau of Sichuan Province at seeding stage.Pratacultural Science,2017,34(1):101-111.
[11]魏云霞,王晓庆,黄洁.PEG胁迫下17份木薯种质苗期抗旱性综合评价及指标筛选.西南农业学报,2016(8):1787-1792.WEI Y X,WANG X Q,HUANG J.Comprehensive evaluation of drought resistance and screening of indices of 17 Cassava germplasms under PEG stress at seedling stage.Southwest China Journal of Agricultural Sciences,2016(8):1787-1792.
[12] BOUREIMA S,OUKARROUM A,DIOUF M,CISSE M,DAMME P V.Screening for drought tolerance in mutant germplasm of sesame(Sesamum indicum)probing by chlorophyll a,fluorescence.Environmental&Experimental Botany,2012,81(3):37-43.
[13]何雪银,文仁来,吴翠荣,周锦国.模糊隶属函数法对玉米苗期抗旱性的分析.西南农业学报,2008(1):52-56.HE X Y,WEN R L,WU C R,ZHOU J G.Analysis of maize drought resistance at seeding stage by fuzzy subordination method.Southwest China Journal of Agricultural Sciences,2008(1):52-56.
[14]逯晓萍,吕学理,张众,王四清.油用红花数量性状的多元遗传分析.中国油料作物学报,2004(2):40-43.LU X P,LYU X L,ZHANG Z,WANG S Q.Multi-genetic analysis of quantitative characters in safflower.Chinese Journal of Oil Crop Sciences,2004(2):40-43.
[15]向长萍,谢军,聂启军,汪李平.23个苦瓜品种(系)农艺性状的主成分分析.华中农业大学学报,2001,20(4):378-381.XIANG C P,XIE J,NIE Q J,WANG L P.Principal component analysis on characters of Balsam Pear.Journal of Huazhong Agricultural University,2001,20(4):378-381.
[16]孟生旺.用主成份分析法进行多指标综合评价应注意的问题.统计研究,1992,9(4):67-68.MENG S W.Problems that should be paid attention to by principal component analysis in multi index comprehensive evaluation.Statistical Research,1992,9(4):67-68.
[17]孙亚昕,巢建国,谷巍,吴启南,刘菊燕.干旱胁迫对不同产地黑三棱生理生化的影响.中药材,2014,37(3):369-371.SUN Y X,CHAO J G,GU W,WU Q N,LIU J Y.The effect of drought stress on the physiological and biochemical characteristics of Sparganium stoloniferum(Graebn.)Buch.from different regions.Journal of Chinese Medicinal Materials,2014,37(3):369-371.
[18]陶玲,任珺.牧草抗旱性综合评价的研究.甘肃农业大学学报,1999(1):23-28.TAO L,REN J.Studies on comprehensive evaluation of forage grass in drought resistance.Journal of Gansu Agricultural University,1999(1):23-28.
[19]万里强,石永红,李向林,何峰,贾亚雄.PEG胁迫下3个多年生黑麦草品种抗性生理研究.草地学报,2009(4):440-444.WANG L Q,SHI Y H,LI X L,HE F,JIA Y X.Physiological resistance of three Lolium perenne L.varieties under PEG stress.Acta Agrestia Sinica,2009(4):440-444.
[20]丁玉梅,马龙海,周晓罡,姚春馨,董禄风,孙茂林.干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析.西南农业学报,2013(1):106-110.DING Y M,MA L H,ZHOU X G,YAO C X,DONG L F,SUN M L.Effects of drought stress on free proline and malonaldedyde contents in potato leaves and correlation analysis of drought-tolerant level among different varieties.Southwest China Journal of Agricultural Sciences,2013(1):106-110.
[21]王彬,李长鼎,马仲泽,黄燕妮,李兴,兰剑.4个高羊茅品种幼苗期抗旱性比较研究.农业科学研究,2011(3):22-26.WANG B,LI C D,MA Z Z,HUANG Y N,LI X,LAN J.Study on drought resistance at seedling stage of 4varieties of tall fescue.Journal of Agricultural Sciences,2011(3):22-26.
[22]石永红,万里强,刘建宁,王运琦,吴欣民,李向林.干旱胁迫对6个坪用多年生黑麦草品种抗旱性的影响.草地学报,2009(1):52-57.SHI Y H,WANG L Q,LIU J N,WANG Y Q,WU X M,LI X L.Effects of PEG stress on the drought resistance of six turfgrass varieties of Lolium perenne L.Acta Agrestia Sinica,2009(1):52-57.
[23]李雪,田新会,杜文华.饲草型小黑麦苗期抗旱指标的筛选.草业科学,2017,34(3):539-546.LI X,TIAN X H,DU W H.Scrccning on the drought resistance index and conditions for×Triticale Wittmack at the seedling stage.Pratacultural Science,2017,34(3):539-546.
[24]崔茂盛,徐桂芬,徐驰.云南温带多年生优良牧草:扁穗雀麦.草业与畜牧,2011(6):36-37.CUI M S,XU G F,XU C.A quality perennial legume for short-term in temperate zone of Yunnan Province:Bromus catharticus.Prataculture&Animalusbandry,2011(6):36-37.
[25]王志泰,马瑞,马彦军,李毅.利用隶属函数法分析胡枝子抗旱性.干旱区资源与环境,2013(9):119-123.WANG Z T,MA R,MA Y J,LI Y.The drought resistance of 5species of Lespedeza.Journal of Arid Land Resources and Environment,2013(9):119-123.
[2]马啸,周朝杰,张成林,孙铭,郭志慧,王小利,张建波.扁穗雀麦种质资源形态和农艺性状变异的初步分析.草地学报,2015(5):1048-1056.MA X,ZHOU C J,ZHANG C L,SUN M,GUO Z H,WANG X L,ZHANG J B.Patterns of morphological variation and agronomic traits in a worldwide sample of prairie grass gremplasm.Acta Agrestia Sinica,2015(5):1048-1056.
[3]张爱玲,付晨,陈志宏,杨晓鹏,邱盛洁,董青林,严海东,蒋洁,张新全,缪致铭,解关琦,黄琳凯.8份柳枝稷种质资源苗期抗旱性综合评价.草业科学,2017,34(4):706-713.ZHANG A L,FU C,CHEN Z H,YANG X P,QIU S J,DONG Q L,YAN H D,JIANG J,ZHANG X Q,MIU Z M,XIE G Q,HUANG L K.Evaluation of drought resistance in seedlings of eight switchgrass accessions.Pratacultural Science,2017,34(4):706-713.
[4] FAROOP M,WAHID A,KOBAYASHI N,FUJITA D,BASRA SMA.Plant drought stress:effects,mechanisms and management.Agronomy for Sustainable Development,2009,29(1):185-212.
[5]周雪,赵相勇,陈国南.贵州不同地区扁穗雀麦种质资源苗期抗旱性综合评价.贵州畜牧兽医,2016(5):54-58.ZHOU X,ZHAO X Y,CHEN G N.Comprehensive evaluation of drought resistance for Guizhou different regions of Bromus catharticus at the seedling period.Guizhou Journal of Animal Husbandry&Veterinary Medicine,2016(5):54-58.
[6] MWADZINGENI L,SHIMELIS H,DUBE E.Breeding wheat for drought tolerance:Progress and technologies.Journal of Integrative Agriculture,2016,15(5):935-943.
[7] CHEN X,MIN D H,YASIR TA,HU Y G.Evaluation of 14morphological,yield-related and physiological traits as indicators of drought tolerance in Chinese winter bread wheat revealed by analysis of the membership function value of drought tolerance(MFVD).Field Crops Research,2012,137:195-201.
[8]郭郁频,米福贵,闫利军,任永霞,吕世杰,伏兵哲.不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价.草业学报,2014(4):220-228.GUO Y P,MI F G,YAN L J,REN Y X,LYU S J,FU B Z.Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties.Acta Prataculturae Sinica,2014(4):220-228.
[9]曹俊梅,芦静,周安定,刘联正,张新忠,黄天荣,高永红,吴新元.水分胁迫对新疆不同小麦品种幼苗生理特性的影响.新疆农业科学,2014(7):1190-1196.CAO J M,LU J,ZHOU A D,LIU L Z,ZHANG X Z,HUANG T R,GAO Y H,WU X Y.Effects of water stress on the physiological action of different Xinjiang winter wheat varieties in seedling.Xinjiang Agricultural Sciences,2014(7):1190-1196.
[10]刘新,符开欣,郭志慧,张新全,白史且,李达旭,张昌兵,张成林,孙铭,马啸.6份川西北短芒披碱草种质苗期的抗旱性综合评价.草业科学,2017,34(1):101-111.LIU X,FU K X,CUO Z H,ZHANG X Q,BAI S Q,Li D X,ZHANG C B,ZHANG C L,SUN M,MA X.Comprehensive evaluation of drought resistancc in six Elymus breviaristatus accessions from the Northwest Plateau of Sichuan Province at seeding stage.Pratacultural Science,2017,34(1):101-111.
[11]魏云霞,王晓庆,黄洁.PEG胁迫下17份木薯种质苗期抗旱性综合评价及指标筛选.西南农业学报,2016(8):1787-1792.WEI Y X,WANG X Q,HUANG J.Comprehensive evaluation of drought resistance and screening of indices of 17 Cassava germplasms under PEG stress at seedling stage.Southwest China Journal of Agricultural Sciences,2016(8):1787-1792.
[12] BOUREIMA S,OUKARROUM A,DIOUF M,CISSE M,DAMME P V.Screening for drought tolerance in mutant germplasm of sesame(Sesamum indicum)probing by chlorophyll a,fluorescence.Environmental&Experimental Botany,2012,81(3):37-43.
[13]何雪银,文仁来,吴翠荣,周锦国.模糊隶属函数法对玉米苗期抗旱性的分析.西南农业学报,2008(1):52-56.HE X Y,WEN R L,WU C R,ZHOU J G.Analysis of maize drought resistance at seeding stage by fuzzy subordination method.Southwest China Journal of Agricultural Sciences,2008(1):52-56.
[14]逯晓萍,吕学理,张众,王四清.油用红花数量性状的多元遗传分析.中国油料作物学报,2004(2):40-43.LU X P,LYU X L,ZHANG Z,WANG S Q.Multi-genetic analysis of quantitative characters in safflower.Chinese Journal of Oil Crop Sciences,2004(2):40-43.
[15]向长萍,谢军,聂启军,汪李平.23个苦瓜品种(系)农艺性状的主成分分析.华中农业大学学报,2001,20(4):378-381.XIANG C P,XIE J,NIE Q J,WANG L P.Principal component analysis on characters of Balsam Pear.Journal of Huazhong Agricultural University,2001,20(4):378-381.
[16]孟生旺.用主成份分析法进行多指标综合评价应注意的问题.统计研究,1992,9(4):67-68.MENG S W.Problems that should be paid attention to by principal component analysis in multi index comprehensive evaluation.Statistical Research,1992,9(4):67-68.
[17]孙亚昕,巢建国,谷巍,吴启南,刘菊燕.干旱胁迫对不同产地黑三棱生理生化的影响.中药材,2014,37(3):369-371.SUN Y X,CHAO J G,GU W,WU Q N,LIU J Y.The effect of drought stress on the physiological and biochemical characteristics of Sparganium stoloniferum(Graebn.)Buch.from different regions.Journal of Chinese Medicinal Materials,2014,37(3):369-371.
[18]陶玲,任珺.牧草抗旱性综合评价的研究.甘肃农业大学学报,1999(1):23-28.TAO L,REN J.Studies on comprehensive evaluation of forage grass in drought resistance.Journal of Gansu Agricultural University,1999(1):23-28.
[19]万里强,石永红,李向林,何峰,贾亚雄.PEG胁迫下3个多年生黑麦草品种抗性生理研究.草地学报,2009(4):440-444.WANG L Q,SHI Y H,LI X L,HE F,JIA Y X.Physiological resistance of three Lolium perenne L.varieties under PEG stress.Acta Agrestia Sinica,2009(4):440-444.
[20]丁玉梅,马龙海,周晓罡,姚春馨,董禄风,孙茂林.干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析.西南农业学报,2013(1):106-110.DING Y M,MA L H,ZHOU X G,YAO C X,DONG L F,SUN M L.Effects of drought stress on free proline and malonaldedyde contents in potato leaves and correlation analysis of drought-tolerant level among different varieties.Southwest China Journal of Agricultural Sciences,2013(1):106-110.
[21]王彬,李长鼎,马仲泽,黄燕妮,李兴,兰剑.4个高羊茅品种幼苗期抗旱性比较研究.农业科学研究,2011(3):22-26.WANG B,LI C D,MA Z Z,HUANG Y N,LI X,LAN J.Study on drought resistance at seedling stage of 4varieties of tall fescue.Journal of Agricultural Sciences,2011(3):22-26.
[22]石永红,万里强,刘建宁,王运琦,吴欣民,李向林.干旱胁迫对6个坪用多年生黑麦草品种抗旱性的影响.草地学报,2009(1):52-57.SHI Y H,WANG L Q,LIU J N,WANG Y Q,WU X M,LI X L.Effects of PEG stress on the drought resistance of six turfgrass varieties of Lolium perenne L.Acta Agrestia Sinica,2009(1):52-57.
[23]李雪,田新会,杜文华.饲草型小黑麦苗期抗旱指标的筛选.草业科学,2017,34(3):539-546.LI X,TIAN X H,DU W H.Scrccning on the drought resistance index and conditions for×Triticale Wittmack at the seedling stage.Pratacultural Science,2017,34(3):539-546.
[24]崔茂盛,徐桂芬,徐驰.云南温带多年生优良牧草:扁穗雀麦.草业与畜牧,2011(6):36-37.CUI M S,XU G F,XU C.A quality perennial legume for short-term in temperate zone of Yunnan Province:Bromus catharticus.Prataculture&Animalusbandry,2011(6):36-37.
[25]王志泰,马瑞,马彦军,李毅.利用隶属函数法分析胡枝子抗旱性.干旱区资源与环境,2013(9):119-123.WANG Z T,MA R,MA Y J,LI Y.The drought resistance of 5species of Lespedeza.Journal of Arid Land Resources and Environment,2013(9):119-123.