2015-2017年贵阳市禽类环境禽流感病毒污染状况监测
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摘要
目的了解贵阳市禽类环境禽流感病毒污染状况,探讨贵阳市禽流感防控措施。方法 (1)采用随机抽样方法分别抽取贵阳市禽类规模养殖场、散养集中地区、蛋禽批发市场、城乡活禽交易市场等4类禽类环境样本,采用实时荧光定量PCR法检测禽流感病毒核酸(A型、H5、H7、H9亚型)。(2)采用卡方检验对环境禽流感病毒核酸检测结果进行统计分析。结果 4 835份外环境样本的FluA阳性率为16.32%,FluA在各个季度、样本来源、样本类型、流通环节的禽类相关外环境中均有检出,不同年度、不同季度、不同地区、不同样本来源、不同样本类型、不同流通环节的FluA阳性率差异有统计学意义(P<0.001),且在同一份样本中,存在FluA不同亚型混合污染的情况。结论贵阳市禽类相关环境普遍存在禽流感病毒污染,蛋禽批发市场、活禽市场是禽流感病毒持续存在的高风险区域,是禽流感传播的关键环境,应重点监测。
Objective To understand the pollution status of avian influenza virus in the poultry environment of Guiyang,so as to explore the prevention and control measures of avian influenza in the poultry trade in Guiyang.Methods(1)The random sampling method was used to extract respectively samples of four types of poultry environments,including scale poultry farms,free-range inhabited areas,urban and rural live poultry,egg and poultry wholesale market in Guiyang,,using real-time PCR method for detection of avian influenza virus nucleic acid(FluA,H5,H7 and H9 subtype).(2)The nucleic acid test results of various links of environmental avian influenza virus were analyzed by chi-square test.Results The positive rate of FluA in 4835 samples was 16.32%.FluA was detected in each quarter,sample source,the types of samples and all aspects of the circulation.The positive rate of FluA was significantly different in different quarters,different regions,different sample sources,different kinds of samples and different circulations(P<0.000).And there were different subtypes of FluA in the same sample.Conclusion Avian influenza virus contamination is prevalent in a poultry-related environment in Guiyang.Wholesale market and live poultry market are high-risk areas where avian influenza virus persists,and the key environments for the spread of avian flu,and they should be monitored.
Objective To understand the pollution status of avian influenza virus in the poultry environment of Guiyang,so as to explore the prevention and control measures of avian influenza in the poultry trade in Guiyang.Methods(1)The random sampling method was used to extract respectively samples of four types of poultry environments,including scale poultry farms,free-range inhabited areas,urban and rural live poultry,egg and poultry wholesale market in Guiyang,,using real-time PCR method for detection of avian influenza virus nucleic acid(FluA,H5,H7 and H9 subtype).(2)The nucleic acid test results of various links of environmental avian influenza virus were analyzed by chi-square test.Results The positive rate of FluA in 4835 samples was 16.32%.FluA was detected in each quarter,sample source,the types of samples and all aspects of the circulation.The positive rate of FluA was significantly different in different quarters,different regions,different sample sources,different kinds of samples and different circulations(P<0.000).And there were different subtypes of FluA in the same sample.Conclusion Avian influenza virus contamination is prevalent in a poultry-related environment in Guiyang.Wholesale market and live poultry market are high-risk areas where avian influenza virus persists,and the key environments for the spread of avian flu,and they should be monitored.
引文
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[3] 曾乐,洪峰,郑龙超.2016年贵阳市数字化禽蛋配送中心禽类外环境禽流感病毒监测分析[J].现代预防医学,2018,45(16):3036-3039.
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[5] 陈纯,陆剑云,李魁彪,等.气象因素对活禽市场外环境H7N9禽流感病毒影响作用分析[J].首都公共卫生,2017,11(2):56-58.
[6] 张海冰,杨穗娟,彭南秀,等.广州市活禽批发市场禽流感病毒污染状况调查[J].中国动物检疫,2017,34(3):1-4.
[7] 王凤英,朱军礼,方琼楼,等.金华市2011-2013年活禽市场甲型流感病毒监测分析[J].中国预防医学杂志,2015,16(1):14-17.
[8] 陈裕华,何日文,朱建翠,等.广州市活禽批发市场活禽流感感染风险因素调查[J].中国动物检疫,2018,35(5):5-8.
[9] 黄建龙,刘道新,何世成,等.水禽禽流感防控的探讨[J].水禽世界,2012,5(1):10-12.
[10] 章清荷,王海波,侯赛,等.活禽市场休市对人感染H7N9流感防控的作用和影响[J].中国人兽共患病学报,2017,33(10):946-949.
[2] 俞玫,洪峰,石作宏,等.贵阳市2010-2013年活禽市场外环境禽流感病毒监测分析[J].医药前沿,2014(28):372-374.
[3] 曾乐,洪峰,郑龙超.2016年贵阳市数字化禽蛋配送中心禽类外环境禽流感病毒监测分析[J].现代预防医学,2018,45(16):3036-3039.
[4] 曾乐,洪峰,陆朝国,等.贵阳市禽类相关外环境1013份标本中禽流感病毒检测结果分析[J].现代预防医学,2016,43(18):3311-3313,3318.
[5] 陈纯,陆剑云,李魁彪,等.气象因素对活禽市场外环境H7N9禽流感病毒影响作用分析[J].首都公共卫生,2017,11(2):56-58.
[6] 张海冰,杨穗娟,彭南秀,等.广州市活禽批发市场禽流感病毒污染状况调查[J].中国动物检疫,2017,34(3):1-4.
[7] 王凤英,朱军礼,方琼楼,等.金华市2011-2013年活禽市场甲型流感病毒监测分析[J].中国预防医学杂志,2015,16(1):14-17.
[8] 陈裕华,何日文,朱建翠,等.广州市活禽批发市场活禽流感感染风险因素调查[J].中国动物检疫,2018,35(5):5-8.
[9] 黄建龙,刘道新,何世成,等.水禽禽流感防控的探讨[J].水禽世界,2012,5(1):10-12.
[10] 章清荷,王海波,侯赛,等.活禽市场休市对人感染H7N9流感防控的作用和影响[J].中国人兽共患病学报,2017,33(10):946-949.