一株不产生孢子的盐生海芦笋内生真菌鉴定
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摘要
对分离自盐生海芦笋的一株代号为Sal54的内生嗜盐真菌进行核糖体18S rDNA及ITS序列克隆测定,获得长度为1715bp的18SrDNA序列和长度为544bp的ITS序列,其ITS序列在GenBank的登录号为JX292134。获得的基因序列在NCBI数据库上进行同源性比对,构建18S rDNA和ITS系统发育树,并进行系统发育分析,将该菌鉴定为甜菜蛇眼茎点菌Phoma betae。
The sequences of 18S rDNA and ITS gene of a fungal strain named Sal54 isolated from Salicornia bigelovii were cloned and sequenced, and a 18S rDNA gene sequence of 1715 bp in length and an ITS gene sequence of 544 bp in length were obtained. The accession number of the ITS gene sequence in GenBank was JX292134. Homology analysis was conducted. A phylogenetic tree was constructed by using these sequences and the neighbor-joining method. Phylogenetic analysis revealed that the isolated strain was identified as Phoma betae.
The sequences of 18S rDNA and ITS gene of a fungal strain named Sal54 isolated from Salicornia bigelovii were cloned and sequenced, and a 18S rDNA gene sequence of 1715 bp in length and an ITS gene sequence of 544 bp in length were obtained. The accession number of the ITS gene sequence in GenBank was JX292134. Homology analysis was conducted. A phylogenetic tree was constructed by using these sequences and the neighbor-joining method. Phylogenetic analysis revealed that the isolated strain was identified as Phoma betae.
引文
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[15]燕勇,李卫平,高雯洁,等.rDNA-ITS序列分析在真菌鉴定中的应用[J].中国卫生检验杂志,2008(10):1958-1961.
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[21]吴清平,黄龙花,杨小兵,等.核酸序列分析在真菌分类鉴定中的应用[J].中国卫生检验杂志,2009,19(4):959-961.
[22]黄龙花,杨小兵,胡惠萍,等.rDNA部分序列在食用菌进化关系研究中的应用[J].中国卫生检验杂志,2011,21(7):1607-1610.
[23]陈剑山,郑服丛.ITS序列分析在真菌分类鉴定中的应用[J].安徽农业科学,2007,35(13):3785-3786.
[24]LARENA I,SALAZAR O,GONZáLEZ V,et al.Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes[J].Journal of Biotechnology,1999,75:187-194.
[25]李红霞,李国英,任毓忠,等.新疆甜菜苗期病害病原种群的鉴定[J].中国糖料,2001(2):25-29.
[26]WARREN R C.The effect of pollen on the fungal leaf microflora of Beta vulgaris L.and on infection of leaves by Phoma betae[J].Neth J Pl Path,1972,78:89-98.
[27]OIKAWA H,TOSHIMA H,OHASHI S,et al.Diversity of diterpene hydrocarbons in fungus Phoma betae[J].Tetrahedron Letters,2001,42:2329-2332.
[28]OIKAWA H,OHASHI S,ICHIHARA A.Biosynthesis of diterpenoid aphidicolin:isolation of intermediates from P-450 inhibitor treated mycelia of Phoma betae[J].Tetrahedron,1999,55:7541-7554.
[2]赵育卉,杨海燕,辛志宏.盐生海芦笋来源真菌Salcoli6发酵产物抗肿瘤活性成分研究[J].食品科学,2011,32(21):6-11.
[3]KIM Y A,KONG C S,UM Y R,et al.Evaluation of Salicornia herbacea as a potential antioxidant and anti-inflammatory agent[J].J Med Food,2009,12(3):661-668.
[4]JANG H S,KIM K R,CHOI S W,et al.Antioxidant and antithrombus activities of enzyme-treated Salicornia herbacea extracts[J].Ann Nutr Metab,2007,51(2):119-125.
[5]KONG C S,KIM J A,QIAN Z J,et al.Protective effect of isorhamnetin 3-O-betaD-glucopyranoside from Salicornia herbacea against oxidation-induced cell damage[J].Food Chem Toxicol,2009,47(8):1914-1920.
[6]KUNTE H J,TR PER H G,STAN-LOTTER H.Halophilic microorganisms[M].Astrobiology,2002:185-199.
[7]MARGESIN R,SCHINNER F.Potential of halotolerant and halophilic microorganisms for biotechnology[J].Extremophiles,2001,5:73-83.
[8]SEPCIC K,ZALAR P,GUNDE-CIMERMAN N.Low water activity induces the production of bioactive metabolites in halophilic and halotolerant fungi[J].Mar Drugs,2011,9:43-58.
[9]NAZARETH S,GONSALVES V,NAYAK S.A first record of obligate halophilic aspergilli from the dead sea[J].Indian J Microbiol,2012,52(1):22-27.
[10]TONG Shangliang,MIAO Hongzhi.A new species of marine yeast Kluyveromyces penaeid isolated from the heart of penaeid shrimp Penaeus chinensis[J].Journal of the Marine Biological Association of the United Kingdom,1999,79:559-561.
[11]SCHUBERT K,GRESLEBIN A,GROENEWALD J Z,et al.New foliicolous species of Cladosporium from South America[J].Persoonia,2009,22:111-122.
[12]GUO L D,HUANG G R,WANG Y.Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis[J].Mycol Res,2003,107(6):680-688.
[13]LIANG Xiaohui,CAI Yujie,LIAO Xiangru,et al.Isolation and identification of a new hypocrellin A-producing strain Shiraia sp.SUPER-H168[J].Microbiological Research,2009,164(1):9-17.
[14]MCCULLOUGH M J,CLEMONS K V,MCCUSKER J H,et al.Intergenic transcribed spacer PCR ribotyping for differentiation of Saccharomyces species and interspecific hybrids[J].Journal of Clinical Microbiology,1998,36(4):1035-1038.
[15]燕勇,李卫平,高雯洁,等.rDNA-ITS序列分析在真菌鉴定中的应用[J].中国卫生检验杂志,2008(10):1958-1961.
[16]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979:405-419.
[17]郭春秋,罗永兰,张志元.几种真菌的诱导产孢试验[J].海南大学学报:自然科学版,2003,21(1):74-77.
[18]韩凤侠.共同祖先原则和系统发育树的解读[J].生物学通报,2008,43(9):14-15.
[19]HUNT J,BODDY L,RANDERSON P F.An evaluation of 18S rDNA approaches for the study of fungal diversity in grassland soils[J].Microbial Ecology,2004,47:385-395.
[20]STEINER G,M LLER M.What can 18S rDNA do for bivalve phylogeny[J].J Mol Evol,1996,43:58-70.
[21]吴清平,黄龙花,杨小兵,等.核酸序列分析在真菌分类鉴定中的应用[J].中国卫生检验杂志,2009,19(4):959-961.
[22]黄龙花,杨小兵,胡惠萍,等.rDNA部分序列在食用菌进化关系研究中的应用[J].中国卫生检验杂志,2011,21(7):1607-1610.
[23]陈剑山,郑服丛.ITS序列分析在真菌分类鉴定中的应用[J].安徽农业科学,2007,35(13):3785-3786.
[24]LARENA I,SALAZAR O,GONZáLEZ V,et al.Design of a primer for ribosomal DNA internal transcribed spacer with enhanced specificity for ascomycetes[J].Journal of Biotechnology,1999,75:187-194.
[25]李红霞,李国英,任毓忠,等.新疆甜菜苗期病害病原种群的鉴定[J].中国糖料,2001(2):25-29.
[26]WARREN R C.The effect of pollen on the fungal leaf microflora of Beta vulgaris L.and on infection of leaves by Phoma betae[J].Neth J Pl Path,1972,78:89-98.
[27]OIKAWA H,TOSHIMA H,OHASHI S,et al.Diversity of diterpene hydrocarbons in fungus Phoma betae[J].Tetrahedron Letters,2001,42:2329-2332.
[28]OIKAWA H,OHASHI S,ICHIHARA A.Biosynthesis of diterpenoid aphidicolin:isolation of intermediates from P-450 inhibitor treated mycelia of Phoma betae[J].Tetrahedron,1999,55:7541-7554.
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