基于UHPLC-QqQ-MS的四逆散水提液中16种主要化学成分的定量研究
|
摘要
目的建立同时测定四逆散水提液中16种化学成分含量的方法。方法应用UHPLC-QqQ-MS技术,采用负离子模式进行扫描,以MRM配对离子方式进行定量。结果柴胡皂苷A、柴胡皂苷B2、柴胡皂苷C、柴胡皂苷D、橙皮素、儿茶素、甘草苷、甘草酸、甘草素、绿原酸、没食子酸、芍药苷、芍药内酯苷、异甘草素、异绿原酸A、橙皮苷在负离子模式下响应良好。结论本实验所建立的同时测定四逆散水提液中16种化学成分含量的分析方法专属性强、快速、灵敏且准确可靠,在完成含量测定的同时又可以准确给出化合物质量数信息,从而达到成分鉴别的效果,该方法可以为四逆散水提液的全面质量控制提供技术支持。
OBJECTIVE A method for simultaneous determination of 16 chemical components in aqueous extract of Sinisan was established.METHODS UHPLC-QqQ-MS technology was employed.Scan was conducted in negative ion mode and quantitative determination was carried out by MRM paired ions.RESULTS Saikosaponina A,saikosaponina B2,saikosaponina C,saikosaponina D,hesperitin,cianidanol,liquiritin,glycyrrhizic acid,liquiritigenin,chlorogenic acid,gallic acid,paeoniflorin,albiflorin,isoliquiritigenin,isochlorogenic acid A,and hesperidin all have good responses in negative ion mode.CONCLUSION The established method is specific,rapid,sensitive,accurate,and reliable,which can simultaneously determine contents and accurately give mass information of compound to achieve the effects of component identification,and also it can provide references for quality control and evaluation of aqueous extract of Sinisan.
OBJECTIVE A method for simultaneous determination of 16 chemical components in aqueous extract of Sinisan was established.METHODS UHPLC-QqQ-MS technology was employed.Scan was conducted in negative ion mode and quantitative determination was carried out by MRM paired ions.RESULTS Saikosaponina A,saikosaponina B2,saikosaponina C,saikosaponina D,hesperitin,cianidanol,liquiritin,glycyrrhizic acid,liquiritigenin,chlorogenic acid,gallic acid,paeoniflorin,albiflorin,isoliquiritigenin,isochlorogenic acid A,and hesperidin all have good responses in negative ion mode.CONCLUSION The established method is specific,rapid,sensitive,accurate,and reliable,which can simultaneously determine contents and accurately give mass information of compound to achieve the effects of component identification,and also it can provide references for quality control and evaluation of aqueous extract of Sinisan.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:280.
[2]KUMAR A,DOGRA S,PRAKASH A.Protective effect of naringin,a citrus flavonoid,against colchicine-induced cognitive dysfunction and oxidative damage in rats[J].J Med Food,2010,13(4):976-984.
[3]KIM SH,HAN J,SEOG DH,et al.Antidepressant effect of Chaihu-Shugan-San extract and its constituents in rat models of depression[J].Life Sci,2005,76(11):1297-1306.
[4]蔡莉,李荣,吴清清,等.橙皮苷对慢性应激抑郁模型大鼠行为学及HPA轴的影响[J].中国中药杂志,2013,38(2):229-233.
[5]薛红莉,赵鹏.真武汤合逍遥散加减治疗慢性心力衰竭伴发抑郁阳气亏虚兼肝气郁结证[J].中国实验方剂学杂志,2016,22(19):144-148.
[6]王跃生,饶毅,魏惠珍,等.HPLC-“内标”多控法测定四逆散中芍药苷、柚皮苷、橙皮苷、甘草酸和新橙皮苷[J].中草药,2008,39(9):1316-1319.
[7]宗阳,孙冰婷,朱立静,等.高效液相色谱法同时测定四逆散中6种抗抑郁成分含量[J].中国医院药学杂志,2016,36(17):1509-1512.
[8]朱占军,潘瑞乐,斯建勇,等.锥叶柴胡化学成分研究[J].天然产物研究与开发,2008,20(5):833-835.
[9]林东昊,茅仁刚,王智华,等.23种国产柴胡属植物中柴胡皂苷a,c,d含量的RP-HPLC测定[J].药物分析杂志,2004,24(5):479-483.
[10]李玉东,刁勇,王立强.芍药及其有效成分抗肿瘤作用的研究进展[J].海峡药学,2009,21(12):27-32.
[11]张治针,范催生,黄爱华.江西枳实和枳壳的商品质量研究[J].中国中药杂志,1999,24(5):259-262.
[12]张红,孙明江,王凌.枳实的化学成分及药理作用研究进展[J].中草药,2009,32(11):1787-1790.
[13] MARJAN NA, HOSSEIN H.Review of pharmacological effects of Glycyrrhiza sp.and its bioactive compounds[J].Phytother Res,2008,22(6):709-724.
[14]蔡勇敏,孙大鹏,邹杰.论甘草应分生甘草和炙甘草[J].中国中医基础医学杂志,2010,16(12):1164-1166.
[15]王跃飞,文红梅.不同产地甘草的聚类分析[J].中草药,2006,37(3):435-439.
[16]周倩,吕佳,李贵海,等.蜜炙甘草饮片HPLC指纹图谱研究[J].中国中药杂志,2010,35(12):1547-1550.
[2]KUMAR A,DOGRA S,PRAKASH A.Protective effect of naringin,a citrus flavonoid,against colchicine-induced cognitive dysfunction and oxidative damage in rats[J].J Med Food,2010,13(4):976-984.
[3]KIM SH,HAN J,SEOG DH,et al.Antidepressant effect of Chaihu-Shugan-San extract and its constituents in rat models of depression[J].Life Sci,2005,76(11):1297-1306.
[4]蔡莉,李荣,吴清清,等.橙皮苷对慢性应激抑郁模型大鼠行为学及HPA轴的影响[J].中国中药杂志,2013,38(2):229-233.
[5]薛红莉,赵鹏.真武汤合逍遥散加减治疗慢性心力衰竭伴发抑郁阳气亏虚兼肝气郁结证[J].中国实验方剂学杂志,2016,22(19):144-148.
[6]王跃生,饶毅,魏惠珍,等.HPLC-“内标”多控法测定四逆散中芍药苷、柚皮苷、橙皮苷、甘草酸和新橙皮苷[J].中草药,2008,39(9):1316-1319.
[7]宗阳,孙冰婷,朱立静,等.高效液相色谱法同时测定四逆散中6种抗抑郁成分含量[J].中国医院药学杂志,2016,36(17):1509-1512.
[8]朱占军,潘瑞乐,斯建勇,等.锥叶柴胡化学成分研究[J].天然产物研究与开发,2008,20(5):833-835.
[9]林东昊,茅仁刚,王智华,等.23种国产柴胡属植物中柴胡皂苷a,c,d含量的RP-HPLC测定[J].药物分析杂志,2004,24(5):479-483.
[10]李玉东,刁勇,王立强.芍药及其有效成分抗肿瘤作用的研究进展[J].海峡药学,2009,21(12):27-32.
[11]张治针,范催生,黄爱华.江西枳实和枳壳的商品质量研究[J].中国中药杂志,1999,24(5):259-262.
[12]张红,孙明江,王凌.枳实的化学成分及药理作用研究进展[J].中草药,2009,32(11):1787-1790.
[13] MARJAN NA, HOSSEIN H.Review of pharmacological effects of Glycyrrhiza sp.and its bioactive compounds[J].Phytother Res,2008,22(6):709-724.
[14]蔡勇敏,孙大鹏,邹杰.论甘草应分生甘草和炙甘草[J].中国中医基础医学杂志,2010,16(12):1164-1166.
[15]王跃飞,文红梅.不同产地甘草的聚类分析[J].中草药,2006,37(3):435-439.
[16]周倩,吕佳,李贵海,等.蜜炙甘草饮片HPLC指纹图谱研究[J].中国中药杂志,2010,35(12):1547-1550.