四逆散高效液相色谱质量标准的建立
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摘要
目的研究四逆散的质量标准。方法用高效液相色谱(HPLC)法,梯度洗脱,用Kromasil C_(18)柱(4. 6 mm×250 mm,5μm),流速1. 0 mL·min~(-1),柱温30℃,流动相为乙腈-0. 05%磷酸水。结果四逆散中的主要有效成分和含量:芍药苷2. 44%、柴胡皂甙A2. 12%、柚皮苷3. 02%、甘草次酸2. 36%。10批四逆散样品在240 nm和210 nm分别确定了20,24个共有峰,且相似度分析结果均在0. 99以上。结论用HPLC方法测定四逆散中主要化学成分的含量及指纹图谱研究,方法可靠,操作简单,可用于全面控制四逆散的质量。
Objective To study the quality standard of Si-Ni-San Powder. Methods Using high performance liquid chromatography( HPLC),gradient elution,Kromasil C_(18) column( 4. 6 mm × 250 mm,5μm),flow rate 1. 0 mL · min~(-1),column temperature 30 ℃,mobile phase acetonitrile-0. 05% phosphoric acid water. Results The contents of the main effective components in Si-Ni-San Powder were as follows: paeoniflorin 2. 44%,saikosaponin A2. 12%,naringin 3. 02%,and glycyrrhetinic acid 2. 36%. The 10 batches of Si-Ni-San scattered samples at 240 nm and 210 nm,respectively 20,24 consensus peaks were identified,and similarity analysis results were all above 0. 99. Conclusion The HPLC method was used to determine the content of main chemical components in Si-Ni-San Powder. The method is reliable,simple in operation,reproducible,fingerprint characteristics and specificity,and can be used to fully control the quality of Si-Ni-San Powder.
Objective To study the quality standard of Si-Ni-San Powder. Methods Using high performance liquid chromatography( HPLC),gradient elution,Kromasil C_(18) column( 4. 6 mm × 250 mm,5μm),flow rate 1. 0 mL · min~(-1),column temperature 30 ℃,mobile phase acetonitrile-0. 05% phosphoric acid water. Results The contents of the main effective components in Si-Ni-San Powder were as follows: paeoniflorin 2. 44%,saikosaponin A2. 12%,naringin 3. 02%,and glycyrrhetinic acid 2. 36%. The 10 batches of Si-Ni-San scattered samples at 240 nm and 210 nm,respectively 20,24 consensus peaks were identified,and similarity analysis results were all above 0. 99. Conclusion The HPLC method was used to determine the content of main chemical components in Si-Ni-San Powder. The method is reliable,simple in operation,reproducible,fingerprint characteristics and specificity,and can be used to fully control the quality of Si-Ni-San Powder.
引文
[1]李廷利,朱维莉,齐凤琴,等.四逆散催眠作用的实验研究[J].中医药信息,2004,21(1):59-60.
[2]郭际.四逆散有效成分提取和成分分析研究[D].成都:成都中医药大学,2002.
[3]李春成.复方四逆散活性部位分离纯化的研究[D].南京:南京师范大学,2005.
[4]李越峰,李廷利.四逆散中主要有效成分芍药苷对睡眠影响的实验研究[J].中成药,2011,33(4):690-692.
[5]李越峰,严兴科,余琰.四逆散指纹图谱相似度的分析研究[J].时珍国医国药,2011,22(5):1042-1044.
[2]郭际.四逆散有效成分提取和成分分析研究[D].成都:成都中医药大学,2002.
[3]李春成.复方四逆散活性部位分离纯化的研究[D].南京:南京师范大学,2005.
[4]李越峰,李廷利.四逆散中主要有效成分芍药苷对睡眠影响的实验研究[J].中成药,2011,33(4):690-692.
[5]李越峰,严兴科,余琰.四逆散指纹图谱相似度的分析研究[J].时珍国医国药,2011,22(5):1042-1044.