巨紫荆种子休眠原因及破眠技术研究
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摘要
为了探究巨紫荆种子休眠的原因以及打破休眠的方法,通过形态解剖、破损种皮以及赤霉素处理等方法,明确巨紫荆种子休眠原因及破除休眠的最佳方法。结果表明:巨紫荆种子存在明显硬实现象,由于机械障碍,水分难以透过种皮,空瘪、虫蛀率等较高。巨紫荆种子休眠主要是由种皮限制所致,胚根存在生理休眠,切除胚根端结合赤霉素处理可以快速打破休眠。赤霉素50～150mg/L处理对种子萌发有很好的促进作用。巨紫荆种子清水浸泡3d,切除种子胚根端2mm左右,再用100mg/L赤霉素浸泡12h,为快速有效打破种子休眠促使萌发的最佳方法,第3天开始萌发,第7天发芽率可达90%以上。
In order to explore the cause of the seed dormancy of C.giganteaand the method of breaking dormancy,anatomical shape,mechanical damage and gibberellin treatment methods were taken to know the causes of the seed dormancy and take corresponding measures to break the seed dormancy.The results showed that there were obvious hard phenomena and mechanical obstacles in the seeds of C.gigantea.The dormancy of C.gigantea seed was mainly caused by the restriction of seed coat,and the radicle had a physiological dormancy,and the resection of the part radicle combined with the treatment of gibberellin could quickly break the dormancy.The treatment of gibberellin 50-150mg/L were beneficial to seed germination.After three days for soaking seeds,the radicle was removed about2 mm,then,the seeds were soaked with 100mg/L gibberellin for 12 h,which was the best way to break the seed dormancy and promoted germination quickly and effectively.The seed sprout in third day,and the germination rate could reach more than 90%in seventh day.
In order to explore the cause of the seed dormancy of C.giganteaand the method of breaking dormancy,anatomical shape,mechanical damage and gibberellin treatment methods were taken to know the causes of the seed dormancy and take corresponding measures to break the seed dormancy.The results showed that there were obvious hard phenomena and mechanical obstacles in the seeds of C.gigantea.The dormancy of C.gigantea seed was mainly caused by the restriction of seed coat,and the radicle had a physiological dormancy,and the resection of the part radicle combined with the treatment of gibberellin could quickly break the dormancy.The treatment of gibberellin 50-150mg/L were beneficial to seed germination.After three days for soaking seeds,the radicle was removed about2 mm,then,the seeds were soaked with 100mg/L gibberellin for 12 h,which was the best way to break the seed dormancy and promoted germination quickly and effectively.The seed sprout in third day,and the germination rate could reach more than 90%in seventh day.
引文
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[3]刘念中,王乐平.巨紫荆优良新品系实生与嫁接快速繁殖技术[J].安徽农业科学,2011,39(30):18 660,18 674.
[4]张林,刘念中.巨紫荆优良新品系组织培养快速繁殖技术[J].安徽农业科学,2012,40(2):860-862.
[5]周瑞玲,卢芳,蔡枫,等.巨紫荆在徐州地区的引种栽培与繁殖试验[J].江苏农业科学,2010(3):250-251.
[6]范军科,孙士杰.优美的园林观赏树种---巨紫荆[J].林业实用技术,2004(8):43.
[7]金继良,夏培兴.不同处理对巨紫荆种子发芽率的影响[J].南方林业科学,2015,43(5):5-6,42.
[8]卢芳,周瑞玲,蔡枫.GA3处理与层积时间对巨紫荆种子萌发的影响[J].安徽农业科学,2007(21):6 419-6 420.
[9]刘雅琦,张建,李刚,等.对节白蜡种子发芽特性的研究[J].种子,2016,35(10):27-31.
[10]米建华,孙雪霞,娄秋莲,等.打破紫荆种子休眠方法研究[J].河南农业科学,2016,45(11):100-104.
[11]Juan Niu,Da Cao,Haoxian Li,et al.Quantitative proteomics of pomegranate varieties with contrasting seed hardness during seed development stages[J].Tree Genetics&Genomes,2018,14:14-26.
[12]杨期和,尹小娟,叶万辉.硬实种子休眠的机制和解除方法[J].植物学通报,2006(1):108-118.
[13]Emine Burcu Ye爧ilyurt,SadIk Erik,9agˇatay Tav爧ano gˇlu Inter-population variability in seed dormancy,seed mass and germination in Helianthemum salicifolium(Cistaceae),a hardseeded annual herb[J].Folia Geobot,2017,52:253-263.
[14]黄利春.河西地区三种豆科牧草主要传粉昆虫及结实特性研究[D].兰州大学,2013.
[15]高成杰,杨文云,刘方炎,等.昆虫寄生与滇合欢种子大小和数量的关系[J].环境昆虫学报,2015,37(5):939-945.