正极性驻极体5-氟尿嘧啶贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TCF-β表达的影响
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摘要
目的研究+5 kV驻极体、5-氟尿嘧啶(5-FU)贴剂和+5 kV驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和转化生长因子-β(TGF-β)表达的影响。方法将通过电晕充电系统制备的正极性聚丙烯驻极体充电面与制备的5-FU贴剂背衬层结合制成表面电位为+5 kV的驻极体5-FU贴剂,再将+5 kV驻极体、5-FU贴剂和+5 kV驻极体5-FU贴剂分别作用于兔耳创面,通过测算瘢痕增生指数,观察研究瘢痕增生状况,通过HE染色、Masson染色和免疫组织化学的方法,研究上述驻极体、5-FU贴剂和驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TGF-β表达的影响。结果①兔耳创面自然愈合4周后形成增生性瘢痕组织。②正极性驻极体不会导致创面过度修复。③5-FU贴剂和正极性驻极体5-FU贴剂通过减少瘢痕组织内Ⅰ型胶原、Ⅲ型胶原和TGF-β的表达量,抑制增生性瘢痕的生长。④正极性驻极体5-FU贴剂抑制增生性瘢痕生长的效果优于5-FU贴剂。结论正极性驻极体与5-FU联用抑制增生性瘢痕生长的效果更好。
Objective To study the effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue.Methods The positive electret 5-fluorouracil(5-FU) patches with surface potential of+5 kV were made by attaching the positive charged polypropylene(PP) electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagen and TGF-β in the rabbit ear scar tissue.Results ① The hypertrophic scar(HS) was formed after 4 weeks natural healing.② The positive electret will not lead to excessive wound repair.③ Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of type Ⅰ collagen,type Ⅲ collagen and TGF-β in scar tissue and inhibited the growth of HS.④ The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch.Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.
Objective To study the effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue.Methods The positive electret 5-fluorouracil(5-FU) patches with surface potential of+5 kV were made by attaching the positive charged polypropylene(PP) electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagen and TGF-β in the rabbit ear scar tissue.Results ① The hypertrophic scar(HS) was formed after 4 weeks natural healing.② The positive electret will not lead to excessive wound repair.③ Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of type Ⅰ collagen,type Ⅲ collagen and TGF-β in scar tissue and inhibited the growth of HS.④ The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch.Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.
引文
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[15] 孔德平,陈凯.5-氟尿嘧啶对增生性瘢痕的作用研究[J],重庆医学,2015,44(8):1029-1031.
[16] 杨卫玺,杨敏烈,吕国忠.药物注射法治疗60例增生性瘢痕的疗效观察[J],中国美容医学,2010,l9(3):323-325.
[17] JIANG J,LIANG Y,DONG F,et al.Study of electret effect of rat skin by thermally stimulated discharge analysis[J].J Electrostat,2012,70(3): 258-263.
[18] 梁媛媛,江键,程亮,等.负极性聚丙烯驻极体美洛昔康贴剂对大鼠皮肤结构的影响[J].解剖学杂志,2009,32(4):502-505.
[19] 苑旺,梁媛媛,崔黎丽,等.正极性聚丙烯驻极体对糖尿病大鼠皮肤结构的影响[J].中国医学物理学杂志,2015,32(6):835-840.
[20] TU Y,WANG X,LU Y,et al.Promotion of the transdermal delivery of protein drugs by N-trimethyl chitosan nanoparticles combined with polypropylene electret[J].Int J Nanomedicine,2016,11: 5549-5561.
[2] LANDEN N X,LI D,STAHLE M.Transition form inflammation to proliferation: a critical step during wound healing[J],Cell Mol Life Sci,2016,73(20): 3861-3885.
[3] ZHANG K,GARNER W,COHEN L,et al.Increased typesⅠand Ⅲ collagen and transforming growth factor-beta 1 mRNA and protein in hypertrophic burn scar[J],J Invest Dermatol,1995,104(5): 750-754.
[4] 周游,王婷,于尔特.增生性瘢痕形成的病理机制和当代治疗现状[J].解剖科学进展,2016,22(4):432-435.
[5] CALISKAN E,GAMSIZKAN M,ACIKGOZ G,et al.Intralesional treatments for hypertrophic scars: comparison among corticosteroid,5-fluorouracil and botulinum toxin in rabbit ear hypertrophic scar model[J].Eur Rev Med Pharmacol Sci,2016,20(8): 1603-1608.
[6] HUSSAIN A,HAQUE M W,SINGH S K,et al.Optimized permeation enhancer for topical delivery of 5-fluorouracil-loaded elastic liposome using design expert: Part Ⅱ [J].Drug Deliv,2016,23(4): 1242-1253.
[7] WANG X,CAO P,LIU J,et.al.5-Aminolaevulinic acid-based photodynamic therapy restrains pathological hyperplasia of fibroblasts [J].Med Sci Monit,2017,23: 46-56.
[8] 王春晓,郭鑫,徐立丽,等.驻极体促进5-氟尿嘧啶体外经皮渗透[J],高电压技术,2016,42(5):1463-1469.
[9] 黄平,石岩,郭鑫,等.正极性驻极体环孢菌素A贴剂的开路热刺激放电[J],高电压技术,2015,41(4): 1390-1394.
[10] CUI L,LIANG Y,MA L,et al.The combinational effect of PP electret and monoolein on transdermal delivery of cyclosporine A in vitro[J].J Electrostat,2013,71(3): 224-227.
[11] CUI L,LIU H,MA L,et al.Enhanced transdermal delivery of cyclosporine A by PP electret and ethyl oleate[J].IEEE Trans Dielectr Electr Insul,2012,19(4): 1191-1194.
[12] 徐立丽.含化学促渗剂驻极体5-FU贴剂体外经皮转运和抑制瘢痕生长的动物实验研究[D].上海:第二军医大学,2017:14-49.
[13] 李荟元.瘢痕的药物治疗进展[J],中国临床康复,2002,6(8):1090-1092.
[14] 郝冬月,李小静.5-氟尿嘧啶在病理性瘢痕治疗中的应用进展[J],中华医学美学美容杂志,2010,16(4):286-288.
[15] 孔德平,陈凯.5-氟尿嘧啶对增生性瘢痕的作用研究[J],重庆医学,2015,44(8):1029-1031.
[16] 杨卫玺,杨敏烈,吕国忠.药物注射法治疗60例增生性瘢痕的疗效观察[J],中国美容医学,2010,l9(3):323-325.
[17] JIANG J,LIANG Y,DONG F,et al.Study of electret effect of rat skin by thermally stimulated discharge analysis[J].J Electrostat,2012,70(3): 258-263.
[18] 梁媛媛,江键,程亮,等.负极性聚丙烯驻极体美洛昔康贴剂对大鼠皮肤结构的影响[J].解剖学杂志,2009,32(4):502-505.
[19] 苑旺,梁媛媛,崔黎丽,等.正极性聚丙烯驻极体对糖尿病大鼠皮肤结构的影响[J].中国医学物理学杂志,2015,32(6):835-840.
[20] TU Y,WANG X,LU Y,et al.Promotion of the transdermal delivery of protein drugs by N-trimethyl chitosan nanoparticles combined with polypropylene electret[J].Int J Nanomedicine,2016,11: 5549-5561.
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