大豆脲酶基本特性与粉质砂土的固化研究
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摘要
为解决粉土和粉质砂土孔隙较小,限制了微生物固化技术处理时的效果这一问题,在原有生物固化技术的基础上进行改良,采用大豆脲酶处理粉质砂土,开展了大豆脲酶基本特性试验、穿透试验以及粉粒质量分数为15%的粉质砂土试样固化处理试验.证明了大豆脲酶相对于尿素水解细菌更容易穿透粉质砂土,且处理效果更为均匀,底部的碳酸钙质量分数可达3%以上.
To solve the problem of the small porosity of silt and silty sand,which limits the effect of microbial solidification,this paper improved the treatment of silty sand by using soybean urease on the basis of the original microbial solidification technology. The basic properties of soybean urease,the penetration test and the solidification treatment of silty sand samples with 15% silt content were mainly carried out. It is proved that soybean urease could penetrate silty sand more easily than urea hydrolyzed bacteria,the content of calcium carbonate at the bottom of the treatment was more uniform than that of urea hydrolysis bacteria,and the content of calcium carbonate at the bottom of the soil was more than 3%.
To solve the problem of the small porosity of silt and silty sand,which limits the effect of microbial solidification,this paper improved the treatment of silty sand by using soybean urease on the basis of the original microbial solidification technology. The basic properties of soybean urease,the penetration test and the solidification treatment of silty sand samples with 15% silt content were mainly carried out. It is proved that soybean urease could penetrate silty sand more easily than urea hydrolyzed bacteria,the content of calcium carbonate at the bottom of the treatment was more uniform than that of urea hydrolysis bacteria,and the content of calcium carbonate at the bottom of the soil was more than 3%.
引文
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[2]荣辉,钱春香,李龙志.微生物水泥胶结机理[J].硅酸盐学报,2013,41(3):314-319.
[3]程晓辉,杨钻,李萌,等.岩土材料微生物改性的基本方法综述[J].工业建筑,2015,45(7):1-7.
[4]韩智光,程晓辉.营养盐对微生物加固可液化砂土效果的探讨[J].工业建筑,2015,45(7):19-35.
[5]MARTINEZ B C,DEJONG J T,GINN T R,et al.Experimental optimization of microbial-induced carbonate precipitation for soil improvement[J].Journal of Geotechnical and Geoenvironmental Engineering,2013,139(4):587-598.
[6]SOON N W,LEE L M,KHUN T C,et al.Factors affecting improvement in engineering properties of residual soil through microbialinduced calcite precipitation[J].Journal of Geotechnical and Geoenvironmental Engineering,2014,140(5):718-728.
[7]MONTOYA B,DEJONG J.Stress-strain behavior of sands cemented by microbially induced calcite precipitation[J].Journal of Geotechnical and Geoenvironmental Engineering,ASCE,2015,141(6):04015019.
[8]朱志铎.粉土路基稳定理论与工程应用技术研究[D].南京:东南大学,2006.
[9]朱志铎,刘松玉,孙海军.江苏徐宿地区粉土的基本特性及加固方法研究[J].岩土力学,2004,25(7):1155-1158.
[10]刘国彬,王洪新.上海浅层粉砂地层承压水对基坑的危害及治理[J].岩土工程学报,2002,24(6):790-792.
[11]房纯纲,姚成林,贾永梅.堤坝隐患及渗漏无损检测技术与仪器[M].北京:中国水利水电出版社,2010.
[12]占洪波.堤基防渗处理综述[J].治淮,2010(1):14-16.
[13]张铁军,施圆圆,孔令漪,等.黄豆豆渣中脲酶的提取精制及其影响因素研究[J].生物技术进展,2017,7(3):253-257.
[14]崔有宏,罗侃.黄豆脲酶的提取与性质研究[J].甘肃科学学报,2000,12(1):62-66.
[15]周东凯,刘莹,马学良,等.豆酶的提取及其影响因素研究[J].大豆科学,2008,27(4):704-707.
[16]邹菁,喻德忠,杨先进,等.黄豆脲酶的提取及其在缓释肥料中尿素态氮测定中的应用[J].分析科学学报,2004,20(2):178-180.
[17]MARYAM N.Biocementation of sand in geotechnical engineering[D].Singapore:Nanyang Technological University,2014.
[18]WHIFFIN V S.Microbial CaCO3precipitation for the production of biocement[D].Perth West Australia:Morduch University,2004.
[19]何稼,楚剑,刘汉龙,等.微生物岩土技术的研究进展[J].岩土工程学报,2016,38(4):643-653.
[20]CHU J,IVANOV V,HE J,et al.Use of biogeotechnologies for disaster mitigation[M].Lai S:Geotechnics for Catastrophic Flooding Events,2014.
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