巴戟天对小鼠学习记忆障碍的影响

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英文篇名：Effect of Morinda Officinalis extract on learning and memory impairment in mice 作者：叶志雄 ; 肖柳英 ; 潘竞锵 英文作者：Ye Zhixiong;Xiao Liuying;Pan Jingqiang;Panyu Sanatorium;Guangzhou Traditional Chinese Medical Hospital( Institute of Traditional Chinese Medicine); 关键词：巴戟天提取物 ; D-半乳糖 ; 学习记忆 ; 糖基化产物 ; 单胺类神经递质 ; 小鼠 英文关键词：Morinda Officinalis extract;;D-galacitose;;learning and memory;;glycosylation product;;monoamine neurotransmitter;;mouse 中文刊名：GZXI 英文刊名：Academic Journal of Guangzhou Medical University 机构：广州市番禺疗养院;广州市中医医院中医中药研究所; 出版日期：2015-04-15 出版单位：广州医科大学学报 年：2015 期：v.43;No.202 语种：中文; 页：GZXI201502006 页数：6 CN：02 ISSN：44-1710/R 分类号：29-34

摘要

目的:探讨巴戟天提取物对D-半乳糖诱致小鼠学习记忆障碍的改善作用。方法:60只昆明种小鼠完全随机分为6组正常对照组、模型组、石杉碱甲组[0.4 mg/(kg·d)]、巴戟天提取物高剂量组[45g/(kg·d)],巴戟天提取物中剂量组[22.5 g/(kg·d)]和巴戟天提取物低剂量组[11.25 g/(kg·d)],每组10只。上述动物除正常对照组外,均皮下(sc)注射D-半乳糖8周,并于第5周起分别灌胃相应药物。采用水迷宫和自主活动仪测试小鼠学习和记忆能力;采用高效液相检测去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)二羟苯乙酸(DOPAC)的含量;测定血清糖化血红蛋白(Hb A1c)、果糖胺(FRA)、晚期糖基化终末产物(AGEs)和糖基化终末产物受体(RAGEs)以及醛糖还原酶活性水平(AR activity)。结果:巴戟天提取物明显改善模型小鼠学习和记忆能力;各给药组与模型对照组相比单胺类递质含量无明显变化;能明显降低药物处理后引起的AR活性增高,同时巴戟天提取物高、中、低剂量组均明显降低Hb A1c、FRA、AGEs和RAGEs。结论:巴戟天提取物具有改善D-半乳糖诱致小鼠学习记忆障碍的作用,其作用可能与其减少糖基化对脑神经细胞的损伤有关。
        Objective: To investigate the improving effect of Morinda Officinalis extract on learning and memory impairment induced by D-galactose in mice. Methods: Sixty Kunming mice were randomly divided into normal control group,model control group,huperzine A group[0. 4 mg /( kg· d) ],Morinda Officinalis extracthigh dose group [45 g /( kg· d) ],medium dose group[22. 5 g / kg· d) ]and low dose group[11. 25 g·( kg·d) ],with 10 mice in each group. Except the normal control group,all groups received D-galactose by subcutaneous injection for 8 weeks,and gavages of related drugs at the fifth week,respectively. Water maze test and autonomic activity instrument were used to determine the ability of learning and memory in mice. High performance liquid chromatography( HPLC) was used to determine the content of norepinephrine( NE),dopamine( DA), 5-hydroxytryptamine( 5-HT), 5-hydroxyindolacetic acid( 5-HIAA), dihydroxy-phenyl aceticacid( DOPAC) were determined. Also, the serum glycosylated hemoglobin( Hb A1c), fructosamine( FRA),advanced glycosylation end products( AGEs),receptor for advanced glycosylation end products( RAGEs),and aldose reductase activities( AR activity) were determined. Results: Morinda Officinalis extract significantly improved the learning and memory ability in model mice. There were no significant differences in the content of monoamine neurotransmitter between medication groups and model control groups( P > 0. 05).Morinda Officinalis extract can reduce the increased AR activity after medication. Meanwhile,high-medium-low dose groups of Morinda Officinalis extract can significantly decrease Hb A1c、FRA, AGEs and RAGEs.Conclusion: Morinda Officinalis extract can improve the learning and memory impairment induced by D-galactose in mice,which may be related to the reduced glycosylation on the damage of brain cells.

引文

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