走马胎的组织培养与快速繁殖
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摘要
以走马胎(Ardisia gigantifolia)幼嫩茎段为外植体,通过腋芽增殖的方式进行组织培养和快速繁殖研究。结果表明,培养基MS+1.0mg·L~(-1)6-BA+0.2mg·L~(-1) NAA和MS+0.5mg·L~(-1)ZT均可用于腋芽的诱导和前期继代培养,诱导率分别为89.3%和85.7%;芽增殖最佳培养基为MS+0.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) ZT+0.1 mg·L~(-1) NAA,增殖系数为4.3倍;根诱导最佳培养基为1/2MS+1.5 mg·L~(-1) IAA+1.0 mg·L~(-1) NAA,生根率达92.3%,且根系发达,植株健壮;生根苗在混合基质园土:泥炭:珍珠岩=3:1:1 (v/v/v)中移栽成活率为82%。该研究建立了走马胎种苗的组织培养快速繁殖技术体系,且可应用于规模化生产。
In this research, to establish a tissue culture and rapid propagation system of Ardisia gigantifolia, tender stem segments was selected as explants, and axillary buds were used to study proliferation. Culture medium of MS+1.0 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA and MS+0.5 mg·L~(-1) ZT had a significant effect on axillary bud induction and subculture of previous generations; the induction rates were 89.3% and 85.7%, respectively. The best culture medium for the multiplication was MS+0.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) ZT+0.1 mg·L~(-1) NAA, and the propagation coefficient was 4.3. The optimal medium for rooting was 1/2 MS+1.5 mg·L~(-1) IAA+1.0 mg·L~(-1) NAA; the rooting rate reached 92.3%. The roots developed well and plantlets grew vigorously. Plantlets were transplanted to garden soil:peat soil:perlite=3:1:1(v/v/v), and 82% rooted plants survived. The study established a fast propagation system for A. gigantifolia that could be applied to factory production.
In this research, to establish a tissue culture and rapid propagation system of Ardisia gigantifolia, tender stem segments was selected as explants, and axillary buds were used to study proliferation. Culture medium of MS+1.0 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA and MS+0.5 mg·L~(-1) ZT had a significant effect on axillary bud induction and subculture of previous generations; the induction rates were 89.3% and 85.7%, respectively. The best culture medium for the multiplication was MS+0.5 mg·L~(-1) 6-BA+0.1 mg·L~(-1) ZT+0.1 mg·L~(-1) NAA, and the propagation coefficient was 4.3. The optimal medium for rooting was 1/2 MS+1.5 mg·L~(-1) IAA+1.0 mg·L~(-1) NAA; the rooting rate reached 92.3%. The roots developed well and plantlets grew vigorously. Plantlets were transplanted to garden soil:peat soil:perlite=3:1:1(v/v/v), and 82% rooted plants survived. The study established a fast propagation system for A. gigantifolia that could be applied to factory production.
引文
戴卫波,董鹏鹏,梅全喜,何鉴洪,张远军(2018).走马胎石油醚提取物抗类风湿性关节炎的作用机制.中药材41,459-463.
邓小梅,戴小英,万小婷(2003).紫金牛的组织培养.江西林业科技(1),1,24.
丁爱萍,史正军(2010).6-BA对红掌组织培养中红叶变异的影响.植物生理学通讯46,571-573.
符运柳,徐立,李志英,黄碧兰,李克烈(2017).走马胎离体培养及植株再生.北方园艺(4),98-101.
刘拥海,俞乐,丁君辉,王若仲,黄志刚,萧浪涛(2012).植物激素对分枝发育的协同调控作用研究进展.植物生理学报48,941-948.
毛世忠,唐文秀,骆文华,邓涛,丁莉,隗红燕(2010).广西紫金牛属药用植物资源及可持续利用初探.福建林业科技37,119-126.
穆丽华,张静,刘屏(2018).走马胎三萜皂苷衍生物的生物转化制备及其抗肿瘤活性研究.中草药49,1266-1271.
穆丽华,赵海霞,龚强强,周小江,刘屏(2011).走马胎中的三萜皂苷类成分及其体外抗肿瘤活性研究.解放军药学学报27,1-6.
孙英坤,胡绍庆,庞基良,高凯,刘华红,陈焕伟,姚涛,陈林敬,沈柏春(2017).珍稀濒危物种堇叶紫金牛高效快繁体系的建立.植物学报52,764-773.
王清,王蒂,戴朝曦,王玉萍(1997).萘乙酸、2,4-D对马铃薯愈伤组织细胞染色体倍性的影响.甘肃农业大学学报32,304-307.
王燕,汪一婷,吕永平,牟豪杰,李海营,陈剑平(2015).组培增殖方式对网纹草嵌合性状稳定性的影响.植物学报50,372-377.
张宝红,李秀兰,李凤莲,李付广(1996).棉花组织培养中异常苗的发生与转化.植物学报38,845-852.
Barbier F,Péron T,Lecerf M,Perez-Garcia MD,Barrière Q,Rol?ík J,Boutet-Mercey S,Citerne S,Lemoine R,Porcheron B,Roman H,Leduc N,Le Gourrierec J,Bertheloot J,Sakr S(2015).Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida.J Exp Bot 66,2569-2582.
Faiss M,ZalubìlováJ,Strnad M,Schmülling T(1997).Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants.Plant J 12,401-415.
Janssen BJ,Drummond RSM,Snowden KC(2014).Regulation of axillary shoot development.Curr Opin Plant Biol17,28-35.
Mason MG,Ross JJ,Babst BA,Wienclaw BN,Beveridge CA(2014).Sugar demand,not auxin,is the initial regulator of apical dominance.Proc Natl Acad Sci USA 111,6092-6097.
Müller D,Leyser O(2011).Auxin,cytokinin and the control of shoot branching.Ann Bot 107,1203-1212.
Müller D,Waldie T,Miyawaki K,To JPC,Melnyk CW,Kieber JJ,Kakimoto T,Leyser O(2015).Cytokinin is required for escape but not release from auxin mediated apical dominance.Plant J 82,874-886.
邓小梅,戴小英,万小婷(2003).紫金牛的组织培养.江西林业科技(1),1,24.
丁爱萍,史正军(2010).6-BA对红掌组织培养中红叶变异的影响.植物生理学通讯46,571-573.
符运柳,徐立,李志英,黄碧兰,李克烈(2017).走马胎离体培养及植株再生.北方园艺(4),98-101.
刘拥海,俞乐,丁君辉,王若仲,黄志刚,萧浪涛(2012).植物激素对分枝发育的协同调控作用研究进展.植物生理学报48,941-948.
毛世忠,唐文秀,骆文华,邓涛,丁莉,隗红燕(2010).广西紫金牛属药用植物资源及可持续利用初探.福建林业科技37,119-126.
穆丽华,张静,刘屏(2018).走马胎三萜皂苷衍生物的生物转化制备及其抗肿瘤活性研究.中草药49,1266-1271.
穆丽华,赵海霞,龚强强,周小江,刘屏(2011).走马胎中的三萜皂苷类成分及其体外抗肿瘤活性研究.解放军药学学报27,1-6.
孙英坤,胡绍庆,庞基良,高凯,刘华红,陈焕伟,姚涛,陈林敬,沈柏春(2017).珍稀濒危物种堇叶紫金牛高效快繁体系的建立.植物学报52,764-773.
王清,王蒂,戴朝曦,王玉萍(1997).萘乙酸、2,4-D对马铃薯愈伤组织细胞染色体倍性的影响.甘肃农业大学学报32,304-307.
王燕,汪一婷,吕永平,牟豪杰,李海营,陈剑平(2015).组培增殖方式对网纹草嵌合性状稳定性的影响.植物学报50,372-377.
张宝红,李秀兰,李凤莲,李付广(1996).棉花组织培养中异常苗的发生与转化.植物学报38,845-852.
Barbier F,Péron T,Lecerf M,Perez-Garcia MD,Barrière Q,Rol?ík J,Boutet-Mercey S,Citerne S,Lemoine R,Porcheron B,Roman H,Leduc N,Le Gourrierec J,Bertheloot J,Sakr S(2015).Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida.J Exp Bot 66,2569-2582.
Faiss M,ZalubìlováJ,Strnad M,Schmülling T(1997).Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants.Plant J 12,401-415.
Janssen BJ,Drummond RSM,Snowden KC(2014).Regulation of axillary shoot development.Curr Opin Plant Biol17,28-35.
Mason MG,Ross JJ,Babst BA,Wienclaw BN,Beveridge CA(2014).Sugar demand,not auxin,is the initial regulator of apical dominance.Proc Natl Acad Sci USA 111,6092-6097.
Müller D,Leyser O(2011).Auxin,cytokinin and the control of shoot branching.Ann Bot 107,1203-1212.
Müller D,Waldie T,Miyawaki K,To JPC,Melnyk CW,Kieber JJ,Kakimoto T,Leyser O(2015).Cytokinin is required for escape but not release from auxin mediated apical dominance.Plant J 82,874-886.