鹿皮多糖的提取及其保湿性能
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摘要
以鲜鹿皮为原材料,采用复合酶法,通过单因素和正交试验探讨了酶的种类、酶的用量、鹿皮水溶液料液比及提取时间对鹿皮多糖提取率的影响,并采用体外法测定了鹿皮多糖的吸湿性和保湿性。结果表明:鹿皮多糖最佳提取工艺条件为碱性蛋白酶添加量1. 25%,木瓜蛋白酶添加量2. 50%,料液比1∶45 (g∶m L),提取时间3. 0 h,该条件下鹿皮多糖提取率为1. 231%;鹿皮多糖的吸湿性强于透明质酸和聚乙二醇2000,与甘油相近,保湿性强于透明质酸、甘油和聚乙二醇2000。
Using fresh buckskin as raw materials,the effects of enzyme type,enzyme dosage,mass concentration of aqueous solution and extraction time on the extraction rate of buckskin polysaccharides were investigated,by single factor and orthogonal tests,with complex enzyme method. The hygroscopicity and moisture retention of buckskin polysaccharides were measured in vitro method. The experimental results showed that the extraction rate of buckskin polysaccharides was the highest(1. 231%) when the additive amount of alkaline protease and papain were 1. 25% and 2. 50%,the mass concentration of aqueous solution was 1 ∶ 45(g ∶ m L),and the extraction time was 3. 0 h.The hygroscopicity of buckskin polysaccharides was better than polyethylene glycol 2000 and hyaluronic acid,and similar to glycerol. The moisture retention was better than polyethylene glycol 2000,hyaluronic acid and glycerol.
Using fresh buckskin as raw materials,the effects of enzyme type,enzyme dosage,mass concentration of aqueous solution and extraction time on the extraction rate of buckskin polysaccharides were investigated,by single factor and orthogonal tests,with complex enzyme method. The hygroscopicity and moisture retention of buckskin polysaccharides were measured in vitro method. The experimental results showed that the extraction rate of buckskin polysaccharides was the highest(1. 231%) when the additive amount of alkaline protease and papain were 1. 25% and 2. 50%,the mass concentration of aqueous solution was 1 ∶ 45(g ∶ m L),and the extraction time was 3. 0 h.The hygroscopicity of buckskin polysaccharides was better than polyethylene glycol 2000 and hyaluronic acid,and similar to glycerol. The moisture retention was better than polyethylene glycol 2000,hyaluronic acid and glycerol.
引文
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[18]胡欣蕾,田雪梅,李文香,等.花脸香蘑菌丝体多糖提取条件优化及其体外免疫活性[J].食品科学,2017,38(20):185-190.
[2]单云芳,张林源,钟震宇.中草药在养鹿业的应用现状与展望[J].黑龙江畜牧兽医,2013(3):141-143.
[3] Zong A,Cao H,Wang F. Anticancer polysaccharides from natural resources:A review of recent,research[J]. Carbohydrate Polymers,2012,90(4):1395-1410.
[4] Chen Q,Shao X,Ling P,et al. Recent advances in polysaccharides for osteoarthritis therapy[J]. European Journal of Medicinal Chemistry,2017,139:926-935.
[5]李碧婵.多糖的结构和生物学活性研究进展[J].中国西部科技,2008(22):31-32,40.
[6]林俊,李萍,陈靠山.近5年多糖抗肿瘤活性研究进展[J].中国中药杂志,2013,38(8):1116-1125.
[7]赵淑杰,贾亚龙,郑丽宁,等.鹿药多糖的超声提取工艺及含量测定[J].吉林农业大学学报,2017,39(2):238-242.
[8]戴宏杰,孙玉林,杨梅语.拟目乌贼生殖腺碱提多糖的抗氧化及吸湿保湿特性[J].食品科学,2016,37(2):31-38.
[9]张好好,洪波,李宗亮,等.响应面法优化金丝小枣枣渣中多糖提取工艺[J].吉林农业大学学报,2014,36(3):352-359.
[10]齐越.超声波辅助法酶解鹿皮胶原蛋白及其产品研发[D].长春:吉林大学,2014.
[11] Xu Y,Cai F,Yu Z,et al. Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity[J]. Food Chemistry,2016,194:650-658.
[12] Yang J,Zhang H F,Cao X Y,et al. Enzymatic water extraction of polysaccharides fromr,epimedium brevicornur,and their antioxidant activity and protective effect against DNA damage[J]. Journal of Food Biochemistry,2016,41(1):e12298.
[13] Bhotmange D,Wallenius J H,Singhal R S,et al. Enzymatic extraction and characterization of polysaccharide from Tuber aestivum[J]. Bioactive Carbohydrates&Dietary Fibre,2017,10:1-9.
[14] Li S,Han D,Row K H. Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology[J]. Korean Journal of Chemical Engineering,2012,29(5):650-656.
[15] Xu B,Dong Y,Lin L,et al. Determination of momordica charantia L. polysaccharide by improved phenol-sulfuric acid method[J]. Food Science and Technology,2005,7:79-82.
[16]郑必胜,胡康,周立武,等.羧甲基裂褶多糖的制备及其保湿活性研究[J].现代食品科技,2017,33(8):254-261.
[17]董蕊,马卓群,卫功庆,等.林蛙卵胶膜多糖的吸湿保湿性及体外抗氧化性[J].吉林农业大学学报,2018,40(5):633-639.
[18]胡欣蕾,田雪梅,李文香,等.花脸香蘑菌丝体多糖提取条件优化及其体外免疫活性[J].食品科学,2017,38(20):185-190.