细胞外ATP调节NaCl胁迫诱导的烟草悬浮细胞死亡和呼吸抑制
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摘要
本文以烟草悬浮细胞BY-2为材料,探讨了胞外ATP对NaCl诱导的细胞死亡和呼吸抑制的调节作用.实验表明,随着NaCl浓度的逐渐上升(50、100、200、400mmol/L),细胞的死亡水平逐渐上升,而胞外ATP含量和细胞呼吸速率则随NaCl浓度的上升而逐渐下降.本文在200mmol/L NaCl处理下的细胞中探索了胞外ATP对NaCl诱导的细胞死亡和呼吸抑制的调节作用.结果发现,较之200mmol/L NaCl胁迫下的细胞,对NaCl胁迫的细胞加入外源ATP(20μmol/L)其使得细胞死亡水平显著性降低,也使得胞外ATP含量和细胞呼吸速率均有所回升.上述实验观察表明,NaCl胁迫诱导的植物细胞的死亡和呼吸抑制可能和细胞外ATP水平的变化有关,并且胞外ATP对NaCl诱导的细胞死亡具有一定的调节作用.
In the present work,by using BY-2tobacco suspension cells,the effect of the extracellular ATP on the cell death and respiration inhibition induced by NaCl stress were studied.The results showed that with the increment of the concentrations of NaCl(from 50 to 400mmol/L),the level of cell death was increased,while the levels of extracellular ATP and respiratory O2 uptake were decreased.The suspension cells subjected to 200mmol/L NaCl stress were usded to study the effects of the extracellular ATP on the cell death induced by NaCl stress.Compared with the cells under NaCl stress condition(200mmol/L),the NaCl-stressed cells with addition of exogenous ATP(20μmol/L)had lower cell death and higher contents of both extracellular ATP and the respiratory O2 uptake.These results suggest that the cell death and respiration inhibition induced by NaCl stress are related to the change of extracellular ATP,and extracellular ATP can function in mediating the NaCl-induced cell death and respiration inhibition.
In the present work,by using BY-2tobacco suspension cells,the effect of the extracellular ATP on the cell death and respiration inhibition induced by NaCl stress were studied.The results showed that with the increment of the concentrations of NaCl(from 50 to 400mmol/L),the level of cell death was increased,while the levels of extracellular ATP and respiratory O2 uptake were decreased.The suspension cells subjected to 200mmol/L NaCl stress were usded to study the effects of the extracellular ATP on the cell death induced by NaCl stress.Compared with the cells under NaCl stress condition(200mmol/L),the NaCl-stressed cells with addition of exogenous ATP(20μmol/L)had lower cell death and higher contents of both extracellular ATP and the respiratory O2 uptake.These results suggest that the cell death and respiration inhibition induced by NaCl stress are related to the change of extracellular ATP,and extracellular ATP can function in mediating the NaCl-induced cell death and respiration inhibition.
引文
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[14]Dichmann S,Idzko M,Zimpfer U,et al.Adenosine triphosphate-induced oxygen radical production and CD11b up-regulation:Ca++mobilization and actin reorganization in human eosinophils[J].Blood,2000,95:973.
[15]Demidchik V,Shang Z L,Shin R,et al.Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca2+channels[J].Plant J,2009,58:903.
[16]Song C J,Steinebrunner I,Wang X,et al.Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiol,2006,140:1222.
[17]Murashige T,Skoog F.A revised medium for rapid growth and bio assays with tobacco tissue cultures[J].Physiol Plant,1962,15:473.
[18]Guilbault G G,Kramer D N.Fluorometric determination of lipase,acylase,alpha-,and gamma-chymotrypsin and inhibitors of these enzymes[J].Anal Chem,1964,36:409.
[19]Kawai M,Hirofumi U.Coleoptile senescence in rice(Oryza sativa L.)[J].Ann Bot,2000,86:405.
[20]McElroy W D,Strehler B L.Factors influencing the respon se of the biolumines cent reaction to adenosine triphosphate[J].Arch Biochem,1949,22:420.
[21]Bingham I J,Farrar J F.Activity and capacity of respiratory pathways in barley roots deprived of inorganic nutrients[J].Plant Physiol Biochem,1989,27:847.
[22]张士功,刘国栋,刘更另.渗透胁迫和缺磷对小麦幼苗生长的影响[J].植物生理学报,2001,37(2):103.
[23]Curtin D,Steppuhn H.Plant responses to sulfate and chloride Salinity:growth and ionic relations[J].Soil Sci Sco Am J,1993,57:1304.
[24]Chivasa S,Ndimba B K,Simon W J,et al.Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability.Plant Cell,2005,17,3019.
[2]刘奕琳,万福绪,娄晓瑞.盐胁迫对10种墨西哥柏幼苗生理生化的影响[J].南京林业大学学报:自然科学版,2013,37(4):29.
[3]杨升,刘涛,张华新,等.盐胁迫下沙枣幼苗的生长表现和生理特性[J].福建林学院学报,2014,34(1):64.
[4]闫留华,陈敏,王宝山.NaCl胁迫对2种表型盐地碱蓬种子萌发的渗透效应和离子效应研究[J].西北植物学报,2008,28(4):718.
[5]陈莎莎,贺转转,姜生秀,等.藜CaMAPKK2的表达分析及盐胁迫信号通路互作组分的筛选[J].中国农业科学,2013,46(5):889.
[6]Jolivet Y,Pireaux J C.Dizengremel P.Changes in properties of barley leaf mitochondria isolated from NaCl-treated plants[J].Plant Physiol,1990,94:641.
[7]王慧英,孙建设,张建光.NaCl胁迫对苹果砧木K+和Na+吸收的影响及其与耐盐性的关系[J].河北农业大学学报,2002,25(增刊):104.
[8]Khakh B S,Burnstock G.The double life of ATP[J].Sci Am,2009,301:84.
[9]Burnstock G,Kennedy C.Is there a basis for distinguishing two types of P2-purinoceptor[J].Gen Pharmacoi,1985,16(5):433.
[10]Kim S Y,Sivaguru M,Stacey G.Extracellular ATP in plants.Visualization,localization,and analysis of physiological significance in growth and signaling[J].Plant Physiol,2006,142:984.
[11]Thomas C,Rajagopal A,Windsor B,et al.A role for ectophosphatase in xenobiotic resistance[J].Plant Cell,2000,12:519.
[12]Tanaka K,Gilroy S,Jones A M,et al.Extracellular ATP signaling in plants[J].Trends Cell Biol,2010,20:601.
[13]Choi J,Tanaka K,Cao Y,et al.Identification of a plant receptor for extracellular ATP[J].Science,2014,343:290.
[14]Dichmann S,Idzko M,Zimpfer U,et al.Adenosine triphosphate-induced oxygen radical production and CD11b up-regulation:Ca++mobilization and actin reorganization in human eosinophils[J].Blood,2000,95:973.
[15]Demidchik V,Shang Z L,Shin R,et al.Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca2+channels[J].Plant J,2009,58:903.
[16]Song C J,Steinebrunner I,Wang X,et al.Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiol,2006,140:1222.
[17]Murashige T,Skoog F.A revised medium for rapid growth and bio assays with tobacco tissue cultures[J].Physiol Plant,1962,15:473.
[18]Guilbault G G,Kramer D N.Fluorometric determination of lipase,acylase,alpha-,and gamma-chymotrypsin and inhibitors of these enzymes[J].Anal Chem,1964,36:409.
[19]Kawai M,Hirofumi U.Coleoptile senescence in rice(Oryza sativa L.)[J].Ann Bot,2000,86:405.
[20]McElroy W D,Strehler B L.Factors influencing the respon se of the biolumines cent reaction to adenosine triphosphate[J].Arch Biochem,1949,22:420.
[21]Bingham I J,Farrar J F.Activity and capacity of respiratory pathways in barley roots deprived of inorganic nutrients[J].Plant Physiol Biochem,1989,27:847.
[22]张士功,刘国栋,刘更另.渗透胁迫和缺磷对小麦幼苗生长的影响[J].植物生理学报,2001,37(2):103.
[23]Curtin D,Steppuhn H.Plant responses to sulfate and chloride Salinity:growth and ionic relations[J].Soil Sci Sco Am J,1993,57:1304.
[24]Chivasa S,Ndimba B K,Simon W J,et al.Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability.Plant Cell,2005,17,3019.