NaCl胁迫对海岛棉幼苗光合和荧光特性的影响
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摘要
为了给海岛棉耐盐材料的选育、栽培管理提供理论依据,以‘越海9号’和‘PS-7’为材料,从发芽至三叶期进行盐胁迫,分析海岛棉生长指标、光合参数对盐处理的响应。结果表明,盐胁迫降低了海岛棉叶面积、叶绿素含量等生物量积累,提高了叶绿素a/b值;提高了‘越海9号’的净光合速率(Pn)、光化学淬灭系数(qP)等光合荧光参数值,降低了非光化学猝灭系数(NPQ)等参数值;降低了‘PS-7’的Pn、qP等光合、荧光参数值,提高了NPQ等参数值。‘PS-7’的根/茎比值大于‘越海9号’,其余指标均小于‘越海9号’。盐胁迫抑制了海岛棉生长指标及叶绿素含量等生物量积累,对耐盐性弱的品系抑制程度大于耐盐性强的品系。
Aiming at breeding salt-tolerance Gossypium barbadense varieties and promoting cultivationmanagement,using‘Yuehai 9'and‘PS-7'as materials,Na Cl stress was tested from germination stage to 3-leaf stage.The response of growth indexes and photosynthetic parameters to salt was analyzed in Gossypium barbadense.The results showed that salt stress decreased the biomass accumulation such as the leaf area,chlorophyll content of leaves and so on,but increased chlorophyll a/b ratio of Gossypium barbadense.Salt stressenhanced photosynthetic fluorescence parameter values such as net photosynthetic rate(Pn),photochemicalquenching coefficient(qP) and so on,while reduced non-photochemical quenching coefficient(NPQ) and so onin‘Yuehai 9'.Salt stress decreased Pn and qP,but increased NPQ in‘PS-7'.The root/stem ratio in‘PS-7'was greater than that in‘Yuehai 9',and the other indexes of‘PS-7'were less than that of‘Yuehai 9'.Salt stress inhibited the biomass accumulation of Gossypium barbadense such as the growth index,chlorophyllcontent and so on,and the restraining level for low-salt-tolerance strains was greater than that for strong-salt-tolerance strains.
Aiming at breeding salt-tolerance Gossypium barbadense varieties and promoting cultivationmanagement,using‘Yuehai 9'and‘PS-7'as materials,Na Cl stress was tested from germination stage to 3-leaf stage.The response of growth indexes and photosynthetic parameters to salt was analyzed in Gossypium barbadense.The results showed that salt stress decreased the biomass accumulation such as the leaf area,chlorophyll content of leaves and so on,but increased chlorophyll a/b ratio of Gossypium barbadense.Salt stressenhanced photosynthetic fluorescence parameter values such as net photosynthetic rate(Pn),photochemicalquenching coefficient(qP) and so on,while reduced non-photochemical quenching coefficient(NPQ) and so onin‘Yuehai 9'.Salt stress decreased Pn and qP,but increased NPQ in‘PS-7'.The root/stem ratio in‘PS-7'was greater than that in‘Yuehai 9',and the other indexes of‘PS-7'were less than that of‘Yuehai 9'.Salt stress inhibited the biomass accumulation of Gossypium barbadense such as the growth index,chlorophyllcontent and so on,and the restraining level for low-salt-tolerance strains was greater than that for strong-salt-tolerance strains.
引文
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[3]周忠丽,蔡小彦,王春英,等.半野生棉耐盐碱筛选初报[J].中国棉花,2015,42(1):15-18.
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[5]李文昊,王振华,郑旭荣,等.新疆绿洲盐碱滴灌区域荒地土壤盐分变化[J].排灌机械工程学报,2016,34(8):722-729.
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[7]唐薇,罗振,温四民,等.干旱和盐胁迫对棉苗光合抑制效应的比较[J].棉花学报,2007,19(1):28-32.
[8]邱爱华,廖小芳,王灿灿,等.嫁接对提高棉花耐Na Cl胁迫的效应[J].中国农业大学学,2015,20(6):53-60.
[9]张国伟,张雷,唐明星,等.土壤盐分对棉花功能叶气体交换参数和叶绿素荧光参数日变化的影响[J].应用生态学报,2011,22(7):1771-1781.
[10]汪保华,王亚峰,谢晓玲,等.盐胁迫下棉花叶绿素荧光参数的变化[J].湖北农业科学,2014,53(7):1509-1512.
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[12]王俊娟,叶武威,周大云,等.盐胁迫下不同耐盐类型棉花的萌发特性[J].棉花学报,2007,19(4):315-317.
[13]高聚林,赵涛,王志刚,等.高丹草水分利用效率与叶片生理特性的关系[J].作物学报,2007,33(3):455-460.
[14]Powles S B.Photoinhibition of photosynthesis induced by visible light[J].Global Change Biol,2010,16(7):2024-2038.
[15]邹琦.植物生理与生化实验指导[M].北京:中国农业出版社,1995:23-25.
[16]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[17]Munns R.Comparative physiology of salt and water stress[J].Plant Cell Environ,2002,25(2):239-250.
[18]Huang Z R,Long X H,Wang L,et al.Growth photosynthesisan H+-ATPase activity in two lerusalem artichoke varieties under Na Cl-induced stress[J].Process Biochem,2012,47(4):591-596.
[19]Bernstein N,Meiri A,Zilberstaine M.Root growth of Avocado is more sensitive to salinity than shoot growth[J].J Am Soc Hortic Sci,2004,129(2):188-192.
[20]Grotkopp E,Rejmánek M,Rost T L.Toward a causal explanation of plant invasiveness:seeding growth and life-history strategies of29 pine(Pinus)species[J].The Am Nat,2002,159(4):396-419.
[21]Mauchamp A,Mésleard F.Salt tolerance in Phragmites australis populations from coastal Mediterranean marshes[J].Aquat Bot,2001,70(1):39-52.
[22]Salter J,Morris K,Bailey P C E,et al.Interactive effects of salinity and water depth on the growth of Melaleuca ericifolia Sm.(Swamp paperbark)seedlings[J].Aquat Bot,2007,86(3):213-222.
[23]谢德意,王惠萍,王付欣,等.盐胁迫对棉花种子萌发及幼苗生长的影响[J].中国棉花,2000,27(9):12-13.
[24]宁丽华,张大勇,刘佳,等.盐胁迫下苗期栽培大豆生理响应及Na+动态平衡关键基因的表达[J].中国农业科学,2016,49(24):4714-4725.
[25]柯裕州,周金星,卢楠,等.盐胁迫对桑树幼苗光合生理及叶绿素荧光特性的影响[J].林业科学研究,2009,22(2):200-206.
[26]张丽辉,赵骥民,范亚红.中性盐胁迫对高粱苗期光合特性的影响[J].江苏农业科学,2012,40(8):100-101.
[27]Winter K,Schramm M J.Analysis of stomatal and nonstomatal components in the environmental control of CO2exchanges in leaves of Welwitschia mirabilis[J].Plant Physiol,1986,82:173-178.
[28]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annu Rev Plant Physiol,1982,33:317-345.
[29]Yang X H,Chen X Y,Ge Q Y,et al.Tolerance of photosynthesis to photoinhibition,high temperature and drought stress in flag leaves of wheat:A comparison between a hybridization line and its parents grown under field conditions[J].Plant Sci,2006,171:389-397.
[30]Maxwell K,Johnson G N.Chlorophyll fluorescence-a practical guide[J].J Exp Bot,2000,51(345):659-668.
[31]Jiang C D,Gao H Y,Zou Q.Changes of donor and accepter side in photosystem II complex induced by iron deficiency in attached soybean and maize leaves[J].Photosynthetica,2003,41(2):267-271.
[32]Baker N R.A possible role for photosystemⅡin environmental perturbations of photosynthesis[J].Physiol Plantarum,1991,81(4):563-570.
[33]Lu C M,Zhang J H.Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants[J].J Exp Bot,1999,50(336):1199-1206.
[34]Li G,Wan S,Zhou J,et al.Leaf chlorophyll fluorescence,hyperspectral reflectance,pigments content,malondialdehyde and proline accumulation responses of castor bean(ricinus communis L.)seedlings to salt stress levels[J].Ind Crop Prod,2010,31(1):13-19.
[35]Rascher U,Liebig M,Lttge U.Evaluation of instant light-response curves of chlorophyll fluorescence parameters obtained with a portable chlorophyll fluorometer on site in the field[J].Plant Cell Environ,2000,23(12):1397-1405.
[36]Genty B,Briantais J M,Baker N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].BBA-Gen Subjects,1989,990(1):87-92.
[37]乌凤章.Na Cl胁迫对高丛越橘幼苗生长和光合生理特性的影响[J].西北植物学报,2015,35(11):2258-2265.
[38]唐剑,贺忠群,鞠丽萍,等.持续Na Cl胁迫对西洋菜叶绿素荧光参数与电子传递的影响[J].华北农学报,2014,29(6):172-176.
[39]石岱龙,田武英,焦青松,等.外源ATP对Na Cl胁迫下菜豆叶片叶绿素荧光特性的调节[J].广西植物,2016,36(9):1087-1092.
[2]Keiffer C H,Ungar I A.Germination and establishment of halophytes on brine-affected soils[J].J Appl Ecol,2002,39(3):402-415.
[3]周忠丽,蔡小彦,王春英,等.半野生棉耐盐碱筛选初报[J].中国棉花,2015,42(1):15-18.
[4]叶武威,阴祖军,王俊娟,等.旱地盐碱地的利用是中国棉花生产发展的必由出路[A].中国棉花学会2013年年会论文集[C].中国棉花学会,2013:3.
[5]李文昊,王振华,郑旭荣,等.新疆绿洲盐碱滴灌区域荒地土壤盐分变化[J].排灌机械工程学报,2016,34(8):722-729.
[6]杨淑萍,危常州,梁永超.盐胁迫对不同基因型海岛棉光合作用及荧光特性的影响[J].中国农业科学,2010,43(8):1585-1593.
[7]唐薇,罗振,温四民,等.干旱和盐胁迫对棉苗光合抑制效应的比较[J].棉花学报,2007,19(1):28-32.
[8]邱爱华,廖小芳,王灿灿,等.嫁接对提高棉花耐Na Cl胁迫的效应[J].中国农业大学学,2015,20(6):53-60.
[9]张国伟,张雷,唐明星,等.土壤盐分对棉花功能叶气体交换参数和叶绿素荧光参数日变化的影响[J].应用生态学报,2011,22(7):1771-1781.
[10]汪保华,王亚峰,谢晓玲,等.盐胁迫下棉花叶绿素荧光参数的变化[J].湖北农业科学,2014,53(7):1509-1512.
[11]杨淑萍,危常州,梁永超.海岛棉不同基因型幼苗对盐胁迫的生理响应[J].石河子大学学报:自然科学版,2010,28(6):668-673.
[12]王俊娟,叶武威,周大云,等.盐胁迫下不同耐盐类型棉花的萌发特性[J].棉花学报,2007,19(4):315-317.
[13]高聚林,赵涛,王志刚,等.高丹草水分利用效率与叶片生理特性的关系[J].作物学报,2007,33(3):455-460.
[14]Powles S B.Photoinhibition of photosynthesis induced by visible light[J].Global Change Biol,2010,16(7):2024-2038.
[15]邹琦.植物生理与生化实验指导[M].北京:中国农业出版社,1995:23-25.
[16]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[17]Munns R.Comparative physiology of salt and water stress[J].Plant Cell Environ,2002,25(2):239-250.
[18]Huang Z R,Long X H,Wang L,et al.Growth photosynthesisan H+-ATPase activity in two lerusalem artichoke varieties under Na Cl-induced stress[J].Process Biochem,2012,47(4):591-596.
[19]Bernstein N,Meiri A,Zilberstaine M.Root growth of Avocado is more sensitive to salinity than shoot growth[J].J Am Soc Hortic Sci,2004,129(2):188-192.
[20]Grotkopp E,Rejmánek M,Rost T L.Toward a causal explanation of plant invasiveness:seeding growth and life-history strategies of29 pine(Pinus)species[J].The Am Nat,2002,159(4):396-419.
[21]Mauchamp A,Mésleard F.Salt tolerance in Phragmites australis populations from coastal Mediterranean marshes[J].Aquat Bot,2001,70(1):39-52.
[22]Salter J,Morris K,Bailey P C E,et al.Interactive effects of salinity and water depth on the growth of Melaleuca ericifolia Sm.(Swamp paperbark)seedlings[J].Aquat Bot,2007,86(3):213-222.
[23]谢德意,王惠萍,王付欣,等.盐胁迫对棉花种子萌发及幼苗生长的影响[J].中国棉花,2000,27(9):12-13.
[24]宁丽华,张大勇,刘佳,等.盐胁迫下苗期栽培大豆生理响应及Na+动态平衡关键基因的表达[J].中国农业科学,2016,49(24):4714-4725.
[25]柯裕州,周金星,卢楠,等.盐胁迫对桑树幼苗光合生理及叶绿素荧光特性的影响[J].林业科学研究,2009,22(2):200-206.
[26]张丽辉,赵骥民,范亚红.中性盐胁迫对高粱苗期光合特性的影响[J].江苏农业科学,2012,40(8):100-101.
[27]Winter K,Schramm M J.Analysis of stomatal and nonstomatal components in the environmental control of CO2exchanges in leaves of Welwitschia mirabilis[J].Plant Physiol,1986,82:173-178.
[28]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annu Rev Plant Physiol,1982,33:317-345.
[29]Yang X H,Chen X Y,Ge Q Y,et al.Tolerance of photosynthesis to photoinhibition,high temperature and drought stress in flag leaves of wheat:A comparison between a hybridization line and its parents grown under field conditions[J].Plant Sci,2006,171:389-397.
[30]Maxwell K,Johnson G N.Chlorophyll fluorescence-a practical guide[J].J Exp Bot,2000,51(345):659-668.
[31]Jiang C D,Gao H Y,Zou Q.Changes of donor and accepter side in photosystem II complex induced by iron deficiency in attached soybean and maize leaves[J].Photosynthetica,2003,41(2):267-271.
[32]Baker N R.A possible role for photosystemⅡin environmental perturbations of photosynthesis[J].Physiol Plantarum,1991,81(4):563-570.
[33]Lu C M,Zhang J H.Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants[J].J Exp Bot,1999,50(336):1199-1206.
[34]Li G,Wan S,Zhou J,et al.Leaf chlorophyll fluorescence,hyperspectral reflectance,pigments content,malondialdehyde and proline accumulation responses of castor bean(ricinus communis L.)seedlings to salt stress levels[J].Ind Crop Prod,2010,31(1):13-19.
[35]Rascher U,Liebig M,Lttge U.Evaluation of instant light-response curves of chlorophyll fluorescence parameters obtained with a portable chlorophyll fluorometer on site in the field[J].Plant Cell Environ,2000,23(12):1397-1405.
[36]Genty B,Briantais J M,Baker N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].BBA-Gen Subjects,1989,990(1):87-92.
[37]乌凤章.Na Cl胁迫对高丛越橘幼苗生长和光合生理特性的影响[J].西北植物学报,2015,35(11):2258-2265.
[38]唐剑,贺忠群,鞠丽萍,等.持续Na Cl胁迫对西洋菜叶绿素荧光参数与电子传递的影响[J].华北农学报,2014,29(6):172-176.
[39]石岱龙,田武英,焦青松,等.外源ATP对Na Cl胁迫下菜豆叶片叶绿素荧光特性的调节[J].广西植物,2016,36(9):1087-1092.