基于InDel标记的国内绿豆品种遗传多样性分析及指纹图谱构建
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摘要
绿豆是一种抗逆性强、适应性广的作物。本研究选取19个多态性丰富、条带稳定的InDel标记用于42个绿豆参试品种的遗传多样性分析及指纹图谱构建。19个InDel标记有效等位变异数介于1.0998～1.9955之间,平均为1.750;期望杂合度变幅0.0918～0.5049,平均值是0.421;Nei′s基因多样性指数变幅0.0907～0.4989,平均值为0.416。多态性信息含量变幅0.0866～0.3744,0.25 Mungbean(Vigna radiata(L.)Wilczek)represents strong abiotic stress tolerance and wide adaptability.The genetic diversity and fingerprint analysis of 42 mungbean varieties from China were conducted using 19 polymorphic InDel markers.The effective alleles of the InDel markers were between 1.0998-1.9955,with an average of 1.750.The expected heterozygosity(He)was between 0.0918-0.5049,with an average of 0.421.The variation of Nei′s gene diversity index was between 0.0907-0.4989,with an average of 0.416.The value of the polymorphic information content(PIC)was between 0.0866-0.3744,with a mean of 0.325.By using UPGMA,42 varieties were grouped into 2 classes when the genetic similarity coefficient is 0.522.Genetic analysis showed that the the 42 mungbean have been separated from the effect of regional isolation.The DNA fingerprints of 42 mungbean varieties were generated by these polymorphic information and each variety could be accurately identified.Thus,taking advantage of user-friendly InDel markers,our results provided scientific basis for the fingerprint analysis that might be useful in clarification and protection of mungbean varieties.
引文
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[19]赵雅楠,王颖,张东杰.基于SSR标记的黑龙江省绿豆品种遗传多样性分析及指纹图谱构建.食品工业科技,2017,38(19):148-153Zhao Y N,Wang Y,Zhang D J.Genetic diversity analysis and DNA fingerprints construction based on fluorescent labeled SSR Markers for mungbean varieties(Vigna radiate L.)from Heilongjiang province.Science and Technology of Food Industry,2017,38(19):148-153
[20]Chen H,Qiao L,Wang L,Wang S,Blair M W,Cheng X.Assessment of genetic diversity and population structure of mungbean(Vigna radiata)germplasm using EST-based and genomic SSR markers.Gene,2015,566:175-183
[21]刘岩,程须珍,王丽侠,王素华,白鹏,吴传书.基于SSR标记的中国绿豆种质资源遗传多样性研究.中国农业科学,2013,46(20):4197-4209Liu Y,Cheng X Z,Wang L X,Wang S H,Bai P,Wu C S.Genetic diversity research of mungbean germplasm resources by SSR markers in China.Scientia Agricultura Sinica,2013,46(20):4197-4209
[22]梁明山,刘煜,侯留记,李霞.蛋白质电泳指纹图谱对烟草品种鉴定的研究.西南农业学报,2000,13(2):83-88Liang M S,Liu Y,Hou L J,Li X.Studies on protein fingerprints for identification of nicotiana varieties.Southwest China Journal of Agricultural Sciences,2000,13(2):83-88
[2]陈新,袁星星,陈华涛,顾和平,张红梅,崔晓艳,陈玉.绿豆研究最新进展及未来发展方向.金陵科技学院学报,2010,26(2):59-68Chen X,Yuan X X,Chen H T,Gu H P,Zhang H M,Cui X Y,Chen Y.Recent research and future development of mungbean.Journal of Jinling Institute of Technology,2010,26(2):59-68
[3]Keatinge J D H,Yang R Y,Chadha M L,Shanmugasundaram S.Overcoming chronic malnutrition in a future warming world:the key importance of mungbean and vegetable soybean.Euphytica,2011,180(1):129-141
[4]Graham P H,Vance C P.Legumes:importance and constraints to greater use.Plant Physiology,2003,131:872-877
[5]程须珍.绿豆生产技术.北京:中国农业出版社,2016:11-15Cheng X Z.Mungbean production technology.Beijing:China Agriculture Press,2016:11-15
[6]Roy S,Ray B P,Sarker A,Das S C.DNA fingerprinting and genetic diversity in Lentil Germplasm using SSR markers.Asian Journal of Conservation Biology,2015,124(2):109-115
[7]Salimizand H,Menbari S,Ramazanzadeh R,Khonsha M,Saleh Vahedi M.DNA fingerprinting and antimicrobial susceptibility pattern of clinical and environmental Acinetobacter baumannii isolates:a multicentre study.Journal of Chemotherapy,2016,28(4):277-283
[8]吴鑫.柑桔DNA指纹图谱库的构建与分析.重庆:西南大学,2008Wu X.Construction and analysis of DNA fingerprint database for citrus cultivars.Chongqing:Southwest University,2008
[9]Kang Y J,Kim S K,Kim M Y,Lestari P,Kim K H,Ha B K,Jun T H,Hwang W J,Lee T,Lee J,Shim S,Yoon M Y,Jang Y E,Han K S,Taeprayoon P,Yoon N,Somta P,Tanya P,Kim K S,Gwag J G,Moon J K,Lee Y H,Park B S,Bombarely A,Doyle J J,Jackson S A,Schafleitner R,Srinives P,Varshney RK,Lee S H.Genome sequence of mungbean and insights into evolution within Vigna species.Nature Communications,2014,5(5543):5443
[10]Georg Jander,Susan R Norris,Steven D Rounsley,David FBush,Irena M Levin,Robert L.Arabidopsis map-based cloning in the post-genome era.Plant Physiology,2002,129(2):440-450
[11]仪泽会,卢有飞,郭晓芹,惠麦侠,张鲁刚,张明科.大白菜简单序列重复(SSR)和插入/缺失(InDel)标记的开发及通用性分析.农业生物技术学报,2012,20(12):1398-1406Yi Z H,Lu Y F,Guo X Q,Hui M X,Zhang L G,Zhang M K.Development of simple sequence repeat(SSR)and insertion/deletion(InDel)markers in Chinese cabbage(Brassica rapa ssp.pekinesis)and analysis of their transferability.Journal of Agricultural Biotechnology,2012,20(12):1398-1406
[12]Choi I Y,Hyten D L,Matukumalli L K,Song Q,Chaky J M,Quigley C V,Chase K,Lark K G,Reiter R S,Yoon M S,Hwang E Y,Yi S I,Young N D,Shoemaker R C,van Tassell C P,Specht J E,Cregan P B.A soybean transcript map:gene distribution,haplotype and single-nucleotide polymorphism analysis.Genetics,2007,176(1):685-696
[13]Chen J,Somta P,Chen X,Cui X,Yuan X,Srinives P.Gene mapping of a mutant mungbean(Vigna radiata L.)using new molecular markers suggests a gene encoding a YUC4-like protein regulates the chasmogamous flower trait.Frontiers in Plant Science,2016,7(8):830
[14]Fonteles T V,Costa M G M,Jesus A L T D,Fernandes F AN,Rodrigues S.Power ultrasound processing of cantaloupe melon juice:effects on quality parameters.Food Research International,2012,48(1):41-48
[15]Sun M.Genetic diversity in three colonizing orchids with contrasting mating systems.American Journal of Botany,1997,84(2):224-232
[16]张圣平,苗晗,程周超,张忠华,武剑,孙日飞,顾兴芳.黄瓜果实苦味(Bt)基因的插入缺失(Indel)标记.农业生物技术学报,2011,19(4):649-653Zhang S P,Miao H,Cheng Z C,Zhang Z H,Wu J,Sun R F,Gu X F.The Insertion-deletion(Indel)marker linked to the fruit bitterness gene(Bt)in Cucumber.Journal of Agricultural Biotechnology,2011,19(4):649-653
[17]李斯更,沈镝,刘博,邱杨,张晓辉,张忠华,王海平,李锡香.基于黄瓜基因组重测序的InDel标记开发及其应用.植物遗传资源学报,2013,14(2):278-283Li S G,Shen D,Liu B,Qiu Y,Zhang X H,Zhang Z H,Wang H P,Li X X.Development and application of cucumber InDel markers based on genome resequencing.Journal of Plant Genetic resources,2013,14(2):278-283
[18]冯芳君,罗利军,李荧,周立国,徐小艳,吴金红,陈宏伟,陈亮,梅捍卫.水稻InDel和SSR标记多态性的比较分析.分子植物育种,2005,3(5):725-730Feng F J,Luo L J,Li Y,Zhou L G,Xu X Y,Wu J H,Chen HW,Chen L,Mei H W.Comparative analysis of polymorphism of InDel and SSR markers in rice.Molecular Plant Breeding,2005,3(5):725-730
[19]赵雅楠,王颖,张东杰.基于SSR标记的黑龙江省绿豆品种遗传多样性分析及指纹图谱构建.食品工业科技,2017,38(19):148-153Zhao Y N,Wang Y,Zhang D J.Genetic diversity analysis and DNA fingerprints construction based on fluorescent labeled SSR Markers for mungbean varieties(Vigna radiate L.)from Heilongjiang province.Science and Technology of Food Industry,2017,38(19):148-153
[20]Chen H,Qiao L,Wang L,Wang S,Blair M W,Cheng X.Assessment of genetic diversity and population structure of mungbean(Vigna radiata)germplasm using EST-based and genomic SSR markers.Gene,2015,566:175-183
[21]刘岩,程须珍,王丽侠,王素华,白鹏,吴传书.基于SSR标记的中国绿豆种质资源遗传多样性研究.中国农业科学,2013,46(20):4197-4209Liu Y,Cheng X Z,Wang L X,Wang S H,Bai P,Wu C S.Genetic diversity research of mungbean germplasm resources by SSR markers in China.Scientia Agricultura Sinica,2013,46(20):4197-4209
[22]梁明山,刘煜,侯留记,李霞.蛋白质电泳指纹图谱对烟草品种鉴定的研究.西南农业学报,2000,13(2):83-88Liang M S,Liu Y,Hou L J,Li X.Studies on protein fingerprints for identification of nicotiana varieties.Southwest China Journal of Agricultural Sciences,2000,13(2):83-88