2,3,7,8-四氯二苯二■英诱导胎鼠腭裂过程中转化生长因子β受体基因表达变化
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摘要
目的 探讨转化生长因子β受体(TβR-Ⅰ、TβR-Ⅱ及TβR-Ⅲ)基因变化与2,3,7,8-四氯二苯二■恶(TCDD)所致胎鼠腭发育障碍的相关性。方法 将24只C57BL/6 J孕鼠随机分为TCDD组和对照组,每组再根据怀孕时间分成3个亚组,每组4只孕鼠。在妊娠第10日(GD10)一次性分别灌胃20μg/kg TCDD(TCDD组)和等量玉米油(对照组)。在GD13、GD14和GD15分别剖腹取各组胎鼠腭组织,提取总RNA,逆转录成cDNA,利用q-PCR技术检测各组胎鼠腭组织中TβR-Ⅰ、TβR-Ⅱ及TβR-Ⅲ mRNA表达变化。结果 对照组TβR-Ⅰ mRNA相对表达量在GD13、GD14和GD15分别为(2.06±0.21)、(1.38±0.12)和(0.78±0.17);TCDD组分别为(0.98±0.26)、(0.75±0.14)和(1.22±0.05)。对照组TβR-Ⅱ mRNA相对表达量在GD13～GD15分别为(1.84±0.08)、(1.14±0.09)、(0.81±0.13);TCDD组分别为(0.91±0.22)、(0.96±0.06)、(1.30±0.13)。TβR-Ⅲ mRNA在对照组的相对表达量分别为(1.86±0.10)、(1.11±0.11)、(0.67±0.15);TCDD组分别为(0.85±0.05)、(0.93±0.09)、(1.17±0.16)。TCDD组的TβR-Ⅰ、TβR-Ⅱ及TβR-Ⅲ mRNA在GD13和GD14相对表达量均低于对照组(P<0.05);而在GD15,TβR-Ⅰ、TβR-Ⅱ及TβR-Ⅲ mRNA高于对照组(P<0.05)。结论 TCDD诱导的胎鼠在腭发育关键时期GD13与GD14,TβR-Ⅰ、TβR-Ⅱ及TβR-Ⅲ mRNA的低表达很可能参与了TCDD诱发胎鼠腭部融合障碍的过程。
引文
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[15] 王晨.TCDD诱导的胎鼠腭发育过程中DNA甲基化变化[J].中华整形外科杂志,2016,32 (5):372-377.
[16] YUAN X,HE X,ZHANG X,et al.Comparative study of folic acid and alpha-naphthoflavone on reducing TCDD-induced cleft palate in fetal mice [J].Cleft Palate-Craniofacial J,2017,54(2):216-222.
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[2] GAO L Y,WANG Y,YAO Y C,et al.2,3,7,8-Tetrachlorodibenzo-p-dioxin mediated cleft palate by mouse embryonic palate mesenchymal cells [J].Arch Oral Biol,2016,71:150-154.
[3] GAO L Y,ZHANG F Q,ZHAO W H,et al.LncRNA H19 and target gene-mediated cleft palate induced by TCDD [J].BES,2017,30(9):676-680.
[4] MOSSEY P A,LITTLE J,MUNGER R G,et al.Cleft lip and palate[J].Lancet,2009,374(9703):1773-1785.
[5] TARR J T,LAMBI A G,BRADLEY J P,et al.Development of normal and cleft palate:a central role for connective tissue growth factor (CTGF)/CCN2[J].J Dev Biol,2018,6(3):1-23.
[6] ZHAO M,MISHRA L,DENG C X.The role of TGF-beta/SMAD4 signaling in cancer [J].Int J Biol Sci,2018,14(2):111-123.
[7] LI C,LAN Y,JIANG R.Molecular and cellular mechanisms of palate development [J].J Dent Res,2017,96(11):1184-1191.
[8] NIK A M,JOHANSSON J A,GHIAMI M,et al.Foxf2 is required for secondary palate development and Tgfbeta signaling in palatal shelf mesenchyme [J].Develop Biol,2016,415(1):14-23.
[9] HATA A,CHEN Y G.TGF-beta signaling from receptors to Smads[J].Cold Spring Harbor Persp Biol,2016,8(9):1-31.
[10] VANDER ARK A,CAO J,LI X.TGF-β receptors:in and beyond TGF-β signaling [J].Cell Signal,2018,52:112-120.
[11] GAN L Q,FU Y X,LIU X,et al.Transforming growth factor-β3 expression up-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice [J].Toxicol Indust Health,2009,25(7):473-478.
[12] 蒲亚兰.四氯二苯二噁英致胎鼠腭裂作用机制的初步探讨 [J].中华整形外科杂志,2011,6(27):448-452.
[13] 何晓梦.以形态与组织学为基础筛选诱导胎鼠腭裂的四氯二苯二(口恶)英最适剂量[J].卫生研究,2013,42 (2):141-145.
[14] 刘翠苹,袁心刚,傅跃先,等.组蛋白H3乙酰化在TCDD致胎鼠腭裂发生中的作用 [J].中华整形外科杂志,2014,30(5):369-373.
[15] 王晨.TCDD诱导的胎鼠腭发育过程中DNA甲基化变化[J].中华整形外科杂志,2016,32 (5):372-377.
[16] YUAN X,HE X,ZHANG X,et al.Comparative study of folic acid and alpha-naphthoflavone on reducing TCDD-induced cleft palate in fetal mice [J].Cleft Palate-Craniofacial J,2017,54(2):216-222.
[17] BUDI E H,DUAN D,DERYNCK R.Transforming growth factor-beta receptors and smads:regulatory complexity and functional versatility[J].Trends Cell Biol,2017,27(9):658-672.