多金属氧酸盐/壳聚糖磁性复合材料的制备及其用于磷酸化肽的富集
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摘要
建立了一种基于多金属氧酸盐磁性材料富集磷酸化肽的方法。采用层层自组装技术制备多金属氧酸盐/壳聚糖磁性材料,结合基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)检测手段,用于磷酸化肽的富集。该磁性材料具有快速磁响应、亲水性、正电性等优点,对磷酸化肽具有高的富集选择性。实验用β-酪蛋白作为模型蛋白质,通过富集后,方法的检出限为0.02 fmol,说明合成的磁性材料对微量蛋白样品分析具有很高的应用潜力。
A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate(POM) was developed. A layer-by-layer assembly technique was used to prepare magnetic nanoparticles(MNPs) coated with polyoxometalate and chitosan. The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS). The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides. β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique. After the enrichment step, the limit of detection was determined to be 0.02 fmol. The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate(POM) was developed. A layer-by-layer assembly technique was used to prepare magnetic nanoparticles(MNPs) coated with polyoxometalate and chitosan. The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS). The composites exhibited advantages of rapid magnetic separation, hydrophilicity, positive electricity, and a high selectivity for enrichment of phosphopeptides. β-Casein was selected as a model phosphorylated protein to assess the performance of the enrichment technique. After the enrichment step, the limit of detection was determined to be 0.02 fmol. The prepared POM-based MNPs thus exhibit significant potential in the detection of low-abundance phosphoproteins.
引文
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