丹参酮IIA对膀胱癌T24细胞增殖、凋亡和细胞周期蛋白B1、半胱氨酸天冬氨酸蛋白酶-3表达的影响
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摘要
目的探讨丹参酮IIA(TanIIA)对膀胱癌T24细胞株体外增殖、凋亡以及细胞周期蛋白B1(CyclinB1)、半胱氨酸天冬氨酸蛋白酶-3信使核糖核酸(Caspase-3 mRNA)表达的影响。方法体外培养T24细胞,分别给予不同浓度(5 mg/L和10 mg/L)TanIIA,MTT法检测细胞增殖能力,AnnexinV/PI检测细胞凋亡,逆转录-聚合酶链式反应(RTPCR)检测细胞CyclinB1、Caspase-3 mRNA的表达。结果 MTT结果显示:5～10 mg/L TanIIA作用48 h均对T24细胞增殖有抑制作用,对细胞凋亡有诱导作用,而且CyclinB1mRNA下调及Caspase-3 mRNA上调,且均呈浓度依赖性。结论 TanIIA可能通过抑制CyclinB1mRNA和增加Caspase-3mRNA的表达水平,抑制膀胱癌T24细胞增殖及诱导凋亡,发挥抗膀胱癌的作用。
【Objective】To explore the effect of tanshinone ⅡA(TanⅡA) on the proliferation and apoptosis of bladder carcinoma T24 cells and the expression of cyclin B1 and caspase-3 in vitro.【Methods】Bladder cancer T24 cells were cultured and treated with TanⅡA of different concentrations(5 mg/L and 10 mg/L) for 48 hours in vitro. TanⅡA-induced apoptosis was assessed by AnnexinⅡ/PI method and proliferation by MTT. The messenger RNA expression of cyclin B1 and caspase-3 in treated T24 cells and control were detected by real-time PCR.【Results】Treatment with TanⅡA resulted in a marked inhibition of proliferation and an obvious induction effect of apoptosis in treated T-24 cells on a dose-dependent manner. Significant down-regulated and up-regulated messenger RNA expression of cyclin B1 and caspase-3, respectively, in the treated T24 cells compared with the control were found on a dose-dependent manner.【Conclusion】The potential anti-tumor effect of TanⅡA was attested by proliferation inhibition and apoptosis induction of bladder carcinoma cells through reduction in the expression of cyclin B1 and increasing in caspase-3 in vitro.
【Objective】To explore the effect of tanshinone ⅡA(TanⅡA) on the proliferation and apoptosis of bladder carcinoma T24 cells and the expression of cyclin B1 and caspase-3 in vitro.【Methods】Bladder cancer T24 cells were cultured and treated with TanⅡA of different concentrations(5 mg/L and 10 mg/L) for 48 hours in vitro. TanⅡA-induced apoptosis was assessed by AnnexinⅡ/PI method and proliferation by MTT. The messenger RNA expression of cyclin B1 and caspase-3 in treated T24 cells and control were detected by real-time PCR.【Results】Treatment with TanⅡA resulted in a marked inhibition of proliferation and an obvious induction effect of apoptosis in treated T-24 cells on a dose-dependent manner. Significant down-regulated and up-regulated messenger RNA expression of cyclin B1 and caspase-3, respectively, in the treated T24 cells compared with the control were found on a dose-dependent manner.【Conclusion】The potential anti-tumor effect of TanⅡA was attested by proliferation inhibition and apoptosis induction of bladder carcinoma cells through reduction in the expression of cyclin B1 and increasing in caspase-3 in vitro.
引文
[1]GAO S,LIU Z,LI H,et al.Cardiovascular actions and therapeutic potential of tanshinone IIA[J].Atherosclerosis,2012,220(1):3-10.
[2]XU YM,DING GH,HUANG J,et al.Tanshinone IIA pretreatment attenuates ischemia/reperfusion-induced renal injury[J].Experimental and Therapeutic Medicine,2016,12(4):2741-2746.
[3]LIN X,QURESHI MZ,ROMERO MA,et al.Regulation of signaling pathways by tanshinones in different cancers[J].Cellular and Molecular Biology(Noisy-le-Grand,France),2017,63(9):53-58.
[4]STENZL A,COWAN NC,DE SANTIS M,et al.The updated EAUguidelines on muscle-invasive and metastatic bladder cancer[J].Eur Urol,2009,55(4):815-825.
[5]SPIESS PE,AGARWAL LN,BANGS R,et al.Bladder cancer,version 5.2017,NCCN clinical practice guidelines in oncology[J].Journal of the National Comprehensive Cancer Network:JNCCN,2017,15(10):1240-1267.
[6]ZHOU M,ZHOU G,HU S,et al.Tanshinone IIA suppress the proliferation of HNE-1 nasopharyngeal carcinoma an in vitro study[J].Saudi Journal of Biological Sciences,2018,25(2):267-272.
[7]SU CC.Tanshinone IIA inhibits gastric carcinoma AGS cells by decreasing the protein expression of VEGFR and blocking Ras/Raf/MEK/ERK pathway[J].International Journal of Molecular Medicine,2018,41(4):2389-2396.
[8]QIU Y,LI C,WANG Q,et al.Tanshinone IIA induces cell death via Beclin-1-dependent autophagy in oral squamous cell carcinoma SCC-9 cell line[J].Cancer Medicine,2018,7(2):397-407.
[9]ZHANG Y,LI S,HE H,et al.Influence of Tanshinone IIA on apoptosis of human esophageal carcinoma Eca-109 cells and its molecular mechanism[J].Thoracic Cancer,2017,8(4):296-303.
[10]CHIEN SY,KUO SJ,CHEN YL,et al.Tanshinone IIA inhibits human hepatocellular carcinoma J5 cell growth by increasing Bax and Caspase 3 and decreasing CD31 expression in vivo[J].Molecular Medicine Reports,2012,5(1):282-286.
[11]KIM EO,KANG SE,IM CR,et al.Tanshinone IIA induces TRAILsensitization of human lung cancer cells through selective ERstress induction[J].International Journal of Oncology,2016,48(5):2205-2212.
[12]贾未玲,于淼.CyclinB1与肿瘤细胞周期调控[J].哈尔滨商业大学学报(自然科学版),2016,32(6):659-662.
[13]GAVET O,PINES J.Progressive activation of CyclinB1-Cdk1coordinates entry to mitosis[J].Developmental Cell,2010,18(4):533-543.
[14]TIAN HY,LI ZX,LI HY,et al.Effects of 14 single herbs on the induction of Caspase-3 in tumor cells:a brief review[J].Chinese Journal of Integrative Medicine,2013,19(8):636-640.
[2]XU YM,DING GH,HUANG J,et al.Tanshinone IIA pretreatment attenuates ischemia/reperfusion-induced renal injury[J].Experimental and Therapeutic Medicine,2016,12(4):2741-2746.
[3]LIN X,QURESHI MZ,ROMERO MA,et al.Regulation of signaling pathways by tanshinones in different cancers[J].Cellular and Molecular Biology(Noisy-le-Grand,France),2017,63(9):53-58.
[4]STENZL A,COWAN NC,DE SANTIS M,et al.The updated EAUguidelines on muscle-invasive and metastatic bladder cancer[J].Eur Urol,2009,55(4):815-825.
[5]SPIESS PE,AGARWAL LN,BANGS R,et al.Bladder cancer,version 5.2017,NCCN clinical practice guidelines in oncology[J].Journal of the National Comprehensive Cancer Network:JNCCN,2017,15(10):1240-1267.
[6]ZHOU M,ZHOU G,HU S,et al.Tanshinone IIA suppress the proliferation of HNE-1 nasopharyngeal carcinoma an in vitro study[J].Saudi Journal of Biological Sciences,2018,25(2):267-272.
[7]SU CC.Tanshinone IIA inhibits gastric carcinoma AGS cells by decreasing the protein expression of VEGFR and blocking Ras/Raf/MEK/ERK pathway[J].International Journal of Molecular Medicine,2018,41(4):2389-2396.
[8]QIU Y,LI C,WANG Q,et al.Tanshinone IIA induces cell death via Beclin-1-dependent autophagy in oral squamous cell carcinoma SCC-9 cell line[J].Cancer Medicine,2018,7(2):397-407.
[9]ZHANG Y,LI S,HE H,et al.Influence of Tanshinone IIA on apoptosis of human esophageal carcinoma Eca-109 cells and its molecular mechanism[J].Thoracic Cancer,2017,8(4):296-303.
[10]CHIEN SY,KUO SJ,CHEN YL,et al.Tanshinone IIA inhibits human hepatocellular carcinoma J5 cell growth by increasing Bax and Caspase 3 and decreasing CD31 expression in vivo[J].Molecular Medicine Reports,2012,5(1):282-286.
[11]KIM EO,KANG SE,IM CR,et al.Tanshinone IIA induces TRAILsensitization of human lung cancer cells through selective ERstress induction[J].International Journal of Oncology,2016,48(5):2205-2212.
[12]贾未玲,于淼.CyclinB1与肿瘤细胞周期调控[J].哈尔滨商业大学学报(自然科学版),2016,32(6):659-662.
[13]GAVET O,PINES J.Progressive activation of CyclinB1-Cdk1coordinates entry to mitosis[J].Developmental Cell,2010,18(4):533-543.
[14]TIAN HY,LI ZX,LI HY,et al.Effects of 14 single herbs on the induction of Caspase-3 in tumor cells:a brief review[J].Chinese Journal of Integrative Medicine,2013,19(8):636-640.