电针对脑卒中痉挛状态大鼠大脑皮质中谷氨酸与γ-氨基丁酸二者受体表达的影响
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摘要
目的:观察和分析电针对脑卒中痉挛状态(SCA)大鼠大脑皮质中神经兴奋因子谷氨酸(Glu)与神经抑制因子γ-氨基丁酸(GABA)二者含量与表达的影响,从而论证针刺对SCA的缓解作用。方法:将32只SD大鼠随机分为空白组、假手术组、模型组、电针组(模型+电针法针刺阳陵泉、曲池),每组各8只。运用线栓法制作脑卒中痉挛状态模型,造模成功后第1天开始治疗,连续3d。以肌张力评分评价模型和针刺疗效,运用ELISA法分析大鼠大脑皮质中Glu、GABA的含量,RT-PCR法观察大鼠大脑皮质mGluR1a mRNA、GABABR1 mRNA的表达。结果:治疗前,模型组、电针组与空白组、假手术组比较,肌张力评分评分明显升高(P<0.01);治疗后,电针组与模型组比较评分明显降低(P<0.01)。模型组与空白组、假手术组比较Glu含量升高而GABA含量显著降低(P<0.01),电针组与模型组相比Glu含量明显降低而GABA含量显著升高(P<0.01,P<0.05)。模型组与空白组、假手术组比较大脑皮质mGluR1a mRNA的表达明显增强,而GABABR1 mRNA的表达明显减少(P<0.01),针刺组与模型组比较mGluR1a mRNA表达显著减弱,GABABR1 mRNA的表达显著增强(P<0.01)。结论:针刺可调节大脑皮质中Glu低表达和GABA的高表达,从而对SCA产生良好的治疗效果。
Objective: To observe and analyze the effects of electro-acupuncture on the content and expression of neuronal excitatory factor Glu and neuro-inhibitory factor GABA in cerebral cortex of rats with SCA, so as to demonstrate the relieving effect of acupuncture on SCA. Methods: Thirty-two SD rats were randomly divided into control group, sham operation group, model group and electro-acupuncture group with model add electro-acupuncture at Yanglingquan(G34) and Quchi(LI11). There were eight rats in each group. The model of SCA was established by wire bolting method. Treatment started on the first day after successful modeling, for 3 days. The model and acupuncture efficacy were evaluated by muscle tone score, the content of Glu and GABA in cerebral cortex of rats with SCA were analyzed by ELISA, and the mRNA expression of mGluR1 and GABABR1 in the cerebral cortex of rats with SCA were observed by RT-PCR. Results: Compared with the control group and the sham operation group before the treatment, the muscle tone scores were significantly higher in the model group and electro-acupuncture group(P<0.01). After treatment, the score of electro-cupuncture group was significantly lower than model group(P<0.01). Compared with the control group and the sham operation group, Glu content increased and GABA content decreased in the model group(P<0.01), while compared with the model group the content of Glu was decreased and the GABA was increased in the electrocupuncture group(P<0.01, P<0.05). Compared with control group and sham operation group, the mRNA expression of mGluR1 in cerebral cortex was significantly increased in model group, while the mRNA expression of GABABR1 was significantly decreased(P<0.01). Compared with model group, mRNA expression of mGluR1 was decreased and mRNA expression of GABABR1 was increased(P<0.01). Conclusion: Electro-acupuncture could regulate low expression of Glu and high expression of GABA in the cerebral cortex, and thus had a good therapeutic effect on SCA.
Objective: To observe and analyze the effects of electro-acupuncture on the content and expression of neuronal excitatory factor Glu and neuro-inhibitory factor GABA in cerebral cortex of rats with SCA, so as to demonstrate the relieving effect of acupuncture on SCA. Methods: Thirty-two SD rats were randomly divided into control group, sham operation group, model group and electro-acupuncture group with model add electro-acupuncture at Yanglingquan(G34) and Quchi(LI11). There were eight rats in each group. The model of SCA was established by wire bolting method. Treatment started on the first day after successful modeling, for 3 days. The model and acupuncture efficacy were evaluated by muscle tone score, the content of Glu and GABA in cerebral cortex of rats with SCA were analyzed by ELISA, and the mRNA expression of mGluR1 and GABABR1 in the cerebral cortex of rats with SCA were observed by RT-PCR. Results: Compared with the control group and the sham operation group before the treatment, the muscle tone scores were significantly higher in the model group and electro-acupuncture group(P<0.01). After treatment, the score of electro-cupuncture group was significantly lower than model group(P<0.01). Compared with the control group and the sham operation group, Glu content increased and GABA content decreased in the model group(P<0.01), while compared with the model group the content of Glu was decreased and the GABA was increased in the electrocupuncture group(P<0.01, P<0.05). Compared with control group and sham operation group, the mRNA expression of mGluR1 in cerebral cortex was significantly increased in model group, while the mRNA expression of GABABR1 was significantly decreased(P<0.01). Compared with model group, mRNA expression of mGluR1 was decreased and mRNA expression of GABABR1 was increased(P<0.01). Conclusion: Electro-acupuncture could regulate low expression of Glu and high expression of GABA in the cerebral cortex, and thus had a good therapeutic effect on SCA.
引文
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[2]杨慎峭,金荣疆,朱天民,等.康复训练结合电针对脑卒中肢体痉挛大鼠γ-氨基丁酸能中间神经元表达的影响.中国康复医学杂志,2013,28(3):198-203
[3]杨柳,刘玉山,吴宁渤,等.低频重复经颅磁刺激对缺血性脑卒中后抑郁患者血清神经递质、细胞因子的影响.海南医学院学报,2017,23(10):1434-1437
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[6]初晓.基于腱器官“反牵张反射”效应研究按法对于脑卒中后肌痉挛大鼠血浆G1y、GABA含量的影响.长沙:湖南中医药大学,2015
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[10]袁昌杰,黄必德.巨刺联合经筋刺治疗脑卒中后痉挛性偏瘫随机平行对照研究.实用中医内科杂志,2017,31(10):85-88
[11]黄丹霞.脑卒中后肌痉挛的发生率及危险因素调查研究.福州:福建中医药大学,2016
[12]陈紫薇.脑卒中后痉挛性偏瘫证素研究及芍药甘草汤治疗的疗效评价研究.武汉:湖北中医药大学,2016
[13]吴红新.针灸治疗脑卒中后痉挛性偏瘫临床研究.中医学报,2013,28(11):1761-1762
[14]胡德宇,赵明,栗雪梅.电针结合康复训练治疗脑卒中后偏瘫痉挛状态的临床研究.针灸临床杂志,2017,33(7):26-29
[15]杨柳,刘玉山,吴宁渤,等.低频重复经颅磁刺激对缺血性脑卒中后抑郁患者血清神经递质、细胞因子的影响.海南医学院学报,2017,23(10):1434-1437
[2]杨慎峭,金荣疆,朱天民,等.康复训练结合电针对脑卒中肢体痉挛大鼠γ-氨基丁酸能中间神经元表达的影响.中国康复医学杂志,2013,28(3):198-203
[3]杨柳,刘玉山,吴宁渤,等.低频重复经颅磁刺激对缺血性脑卒中后抑郁患者血清神经递质、细胞因子的影响.海南医学院学报,2017,23(10):1434-1437
[4]秦思,金荣疆,文超,等.针刺治疗脑卒中后肢体痉挛调控氨基丁酸转氨酶的研究进展.社区医学杂志,2015,13(7):12-14
[5]朱婷婷.通督治郁针法对卒中后抑郁患者血清GABA水平的影响.合肥:安徽中医药大学,2014
[6]初晓.基于腱器官“反牵张反射”效应研究按法对于脑卒中后肌痉挛大鼠血浆G1y、GABA含量的影响.长沙:湖南中医药大学,2015
[7]李丹.针灸联合电针治疗脑卒中痉挛性瘫痪随机平行对照研究.实用中医内科杂志,2013,27(5):145-146,162
[8]Longa E Z,Weistein P R,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke,1989,20(1):84-91
[9]李忠仁.实验针灸学.北京:中国中医药出版社,2003:329-331
[10]袁昌杰,黄必德.巨刺联合经筋刺治疗脑卒中后痉挛性偏瘫随机平行对照研究.实用中医内科杂志,2017,31(10):85-88
[11]黄丹霞.脑卒中后肌痉挛的发生率及危险因素调查研究.福州:福建中医药大学,2016
[12]陈紫薇.脑卒中后痉挛性偏瘫证素研究及芍药甘草汤治疗的疗效评价研究.武汉:湖北中医药大学,2016
[13]吴红新.针灸治疗脑卒中后痉挛性偏瘫临床研究.中医学报,2013,28(11):1761-1762
[14]胡德宇,赵明,栗雪梅.电针结合康复训练治疗脑卒中后偏瘫痉挛状态的临床研究.针灸临床杂志,2017,33(7):26-29
[15]杨柳,刘玉山,吴宁渤,等.低频重复经颅磁刺激对缺血性脑卒中后抑郁患者血清神经递质、细胞因子的影响.海南医学院学报,2017,23(10):1434-1437