痤疮消霜对兔耳痤疮模型的作用研究
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摘要
目的:观察痤疮消霜对兔耳痤疮模型的影响。方法:选取新西兰兔30只,雌雄不限,分为正常对照组、模型组、痤疮消霜高剂量组、痤疮消霜低剂量组、维A酸霜组共5组。正常对照组3只不涂煤焦油,不用药;余下27只分别外涂煤焦油造模2周,并于造模成功后每天外涂乳膏基质、痤疮消霜(高、低剂量)、0.1%维A霜3周。于末次给药后24小时,以打孔器制作涂药处耳片标本,以10%甲醛溶液固定,苏木精-伊红染色,光学显微镜下观察组织病理学改变,并进行显微照相。结果:27只新西兰兔造模成功24只,未造模成功的3只归为正常对照组。经观察,在改善兔耳痤疮反应强度上,痤疮消霜高剂量组与模型组比较(t=2.62,P<0.05),说明痤疮消霜高剂量能明显降低兔耳痤疮反应强度,有很好的治疗痤疮的作用;痤疮消霜高剂量组与维A酸霜组相比较(t=0.06,P>0.05),两者无统计学差异。同时,在兔耳病理改变上,痤疮消霜高剂量组兔耳病变改善明显,其表皮增厚状况明显减轻,毛囊口扩张程度减轻,毛囊四周角化层明显减轻,炎性细胞减少,与模型组相比(χ~2=4.00,P<0.05),有统计学差异。痤疮消霜低剂量组兔耳病变程度与模型组相比(χ~2=1.09,P>0.05),无统计学差异,未见明显改善。维A酸霜组与痤疮消霜高剂量组相比(χ~2=0.34,P>0.05),无统计学差异。结论:痤疮消霜高剂量组对煤焦油所致兔耳痤疮模型有明显改善作用。
Objective:To observe the effect of acne-eliminating cream on acne in a rabbit ear acne model.Methods:Thirty New Zealand rabbits of both genders were divided into normal control group, acne model group, high and low dose of acne-eliminating cream group, tretinoin cream group. Except 3 rabbits in normal control group, ears of the rest rabbits were treated topically with coal tar for 2 weeks to establish acne model. Afterwards vehicle, acne cream(high and low dose), and 0.1 % tretinoin cream were applied to respective group of rabbit ears daily for 3 weeks. Ear samples were taken 24 hours after the last topical treatment, followed by fixation in 10% formaldehyde and hematoxylin-eosin staining. Histopathological changes were assessed and photographed under a microscope.Results:In comparison to untreated acne ears, high dose of acne-eliminating cream markedly reduced the intensity of ear reactions to coal tar(t=2.62, P<0.05). Moreover, high dose of acne-eliminating cream significantly reduced epidermal thickness, dilation of follicular orifice, perifollicular hyperkeratosis and inflammatory cells( χ~2=4.00,P<0.05 vs. untreated acne ears). However, low-dose of acne-eliminating cream did not dramatically alleviate acne in this model( χ~2=1.09, P>0.05 vs. untreated acne ears). High dose of acne-eliminating cream and tretinoin cream exerted comparable efficacy in reduction of intensity of ear reactions to coal tar and improvements in histopathology(t=0.06, P>0.05 for reduction of intensity; χ~2=0.34, P>0.05 for histopathology).Conclusion:High dose of acne-eliminating cream can significantly improve the acne in coal tar-induced rabbit ear acne model.
Objective:To observe the effect of acne-eliminating cream on acne in a rabbit ear acne model.Methods:Thirty New Zealand rabbits of both genders were divided into normal control group, acne model group, high and low dose of acne-eliminating cream group, tretinoin cream group. Except 3 rabbits in normal control group, ears of the rest rabbits were treated topically with coal tar for 2 weeks to establish acne model. Afterwards vehicle, acne cream(high and low dose), and 0.1 % tretinoin cream were applied to respective group of rabbit ears daily for 3 weeks. Ear samples were taken 24 hours after the last topical treatment, followed by fixation in 10% formaldehyde and hematoxylin-eosin staining. Histopathological changes were assessed and photographed under a microscope.Results:In comparison to untreated acne ears, high dose of acne-eliminating cream markedly reduced the intensity of ear reactions to coal tar(t=2.62, P<0.05). Moreover, high dose of acne-eliminating cream significantly reduced epidermal thickness, dilation of follicular orifice, perifollicular hyperkeratosis and inflammatory cells( χ~2=4.00,P<0.05 vs. untreated acne ears). However, low-dose of acne-eliminating cream did not dramatically alleviate acne in this model( χ~2=1.09, P>0.05 vs. untreated acne ears). High dose of acne-eliminating cream and tretinoin cream exerted comparable efficacy in reduction of intensity of ear reactions to coal tar and improvements in histopathology(t=0.06, P>0.05 for reduction of intensity; χ~2=0.34, P>0.05 for histopathology).Conclusion:High dose of acne-eliminating cream can significantly improve the acne in coal tar-induced rabbit ear acne model.
引文
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[2] 项蕾红,马英.痤疮治疗进展[J].皮肤性病诊疗学杂志,2017,24(4):227-231.
[3] 夏庆梅, 景春晖, 杜天乐.复方中药痤疮凝胶对兔耳痤疮模型治疗作用的实验研究[J] 天津中医药,2014,31(5):296-300.
[4] 江丽,单萍萍,申国庆,等. 中药对痤疮致病菌的体外抑菌活性实验研究[J].药学与临床研究,2014,22(4):315-318.
[5] 王醊恩,林莺,杨毅.痤消膏外治痤疮54例疗效观察[J]. 长春中医药大学学报,2010,26(2):244-245.
[6] 陶迪生,梅令兰.皮炎1号洗液治疗寻常痤疮48例临床疗效观察[J].中国中西医结合皮肤性病学杂志,2004,3(3):171-172.