血脉灵组方对高脂血症大鼠模型血脂和血液流变学的影响
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摘要
目的:探讨血脉灵不同组方对高脂血症大鼠模型血脂和血液流变学的影响。方法:建立高脂血症大鼠模型,同时进行血脉灵组方与阳性对照辛伐他汀治疗,分别于造模开始的第2、4、6周采集大鼠颈外静脉血检测血清中的血脂指标,第6周腹主动脉采血测定全血血液流变学指标。结果:血脉灵(制首乌)和血脉灵(生首乌)组方各剂量组均能不同程度降低高脂模型大鼠给药后血清中的胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL-C)含量,亦能升高血清高密度脂蛋白(HDL-C)值,差异有统计学意义(P<0.05);血脉灵(制首乌)高剂量组能降低高脂模型大鼠的全血黏度(高切和低切)、血浆黏度、高切还原黏度和红细胞聚集指数,低剂量组能降低高切全血黏度、红细胞压积、红细胞聚集指数和红细胞刚性指数,差异有统计学意义(P<0.05);血脉灵(生首乌)高剂量组能降低大鼠的高切全血黏度、血浆黏度和红细胞压积,差异有统计学意义(P<0.05)。结论:血脉灵(制首乌)和血脉灵(生首乌)组方提取物均能明显降低高脂模型大鼠血清脂质,但以含制首乌的血脉灵组方提取物比含制首乌的血脉灵组方提取物效果略强。
Objective:To discuss the effects of XueMaiLing of different prescriptions on blood lipid and hemorheology of rat model with hyperlipemia. Methods: High fat diet was used to feed the rats and establish the model with hyperlipemia, simultaneously, they were treated after separating into the group of XueMaiLing prescription and positive control group of simvastatin, the indexes of blood lipid were detected at the second, forth and sixth week after setting up the models respectively when the blood was drawn from external jugular vein of the rats, the indexes of hemorheology were measured after the blood was taken from abdominal aorta at the sixth week.Results: The prescriptions of XueMaiLing(ZhiShouWu) and XueMaiLing(ShengShouWu) in different dosages could lower the contents of LDL-C, TG and TC of the rat models with hyperlipemia to different degrees after medication,raise HDL-C, and the difference had statistical meaning(P<0.05); high dose of XueMaiLing(ZhiShouWu) could decrease whole blood viscosity(high shear and low shear), plasma viscosity, high shear reduced viscosity and erythrocyte aggregation index, low dose group could reduce high shear whole blood viscosity, hematocrit,erythrocyte aggregation index and erythrocyte rigidity index, and the difference showed statistical meaning(P<0.05);high dose group of XueMaiLing(ShengShouWu) could decrease high shear whole blood viscosity, plasma viscosity and hematocrit, and the difference showed statistical meaning(P <0.05). Conclusion: The extract of XueMaiLing(ZhiShouWu) and XueMaiLing(ShengShouWu) prescriptions cold obviously lower blood lipid of rat model with hyperlipemia, and the extracts of XueMaiLing prescriptions containing ZhiShouWu show stronger effects compared with the extracts of XueMaiLing prescriptions containing ShengShouWu.
Objective:To discuss the effects of XueMaiLing of different prescriptions on blood lipid and hemorheology of rat model with hyperlipemia. Methods: High fat diet was used to feed the rats and establish the model with hyperlipemia, simultaneously, they were treated after separating into the group of XueMaiLing prescription and positive control group of simvastatin, the indexes of blood lipid were detected at the second, forth and sixth week after setting up the models respectively when the blood was drawn from external jugular vein of the rats, the indexes of hemorheology were measured after the blood was taken from abdominal aorta at the sixth week.Results: The prescriptions of XueMaiLing(ZhiShouWu) and XueMaiLing(ShengShouWu) in different dosages could lower the contents of LDL-C, TG and TC of the rat models with hyperlipemia to different degrees after medication,raise HDL-C, and the difference had statistical meaning(P<0.05); high dose of XueMaiLing(ZhiShouWu) could decrease whole blood viscosity(high shear and low shear), plasma viscosity, high shear reduced viscosity and erythrocyte aggregation index, low dose group could reduce high shear whole blood viscosity, hematocrit,erythrocyte aggregation index and erythrocyte rigidity index, and the difference showed statistical meaning(P<0.05);high dose group of XueMaiLing(ShengShouWu) could decrease high shear whole blood viscosity, plasma viscosity and hematocrit, and the difference showed statistical meaning(P <0.05). Conclusion: The extract of XueMaiLing(ZhiShouWu) and XueMaiLing(ShengShouWu) prescriptions cold obviously lower blood lipid of rat model with hyperlipemia, and the extracts of XueMaiLing prescriptions containing ZhiShouWu show stronger effects compared with the extracts of XueMaiLing prescriptions containing ShengShouWu.
引文
[1]赵志宏,王阶.胸痹心痛与冠心病的病证结合及辨证论治[J].中西医结合心脑血管病杂志,2009,7(5):590-592.
[2]李彦斌,张志花,韩立民.冠心病(胸痹心痛)中医文献及病证研究[J].赣南医学院学报,2010,30(2):321-323.
[3]张振千.中医胸痹与冠心病心绞痛的相关性[J].航空航天医药,2010,21(9):1736-1737.
[4]陈宁.芹菜素对动脉粥样硬化大鼠血脂和血液流变学的影响[J].现代中西医结合杂志,2016,25(11):1155-1158.
[5]苏奋翔,张艳霞,安方玉,等.林氏降脂方对高脂血症大鼠血脂和血液流变学的影响[J].西部中医药,2015,28(10):12-14.
[6]胡锡琴,方欢乐,权志博,等.制何首乌对肝脏生化物质影响的实验研究[J].陕西中医学院学报,2007,30(6):63-64.
[7]Xiaoqing Wu,Xiaozhen Chen,Qingchun Huang,et al,Toxicity of raw and processed roots of polygonum multiflorum[J].Fitoterapia 2012,83(3):469-475.
[8]Jie Yu,Jie Xie,Xiao Jian,et al.Hepatoxicity of major constituents and extractions of radix polygoni multiflori and radix polygoni multiflori praeparata[J].Journal of Ethnopharmamcology 2011,137(3):1291-1299.
[9]Cardenas A,Restrepo J,Sierra F.Acute hepatitis due to shen-min:a herbal product de rived from polygonum multiflorum[J].JClinGastroenterol2006,40(7):629-632.
[10]闫二磊,梁生旺.何首乌肝损伤的研究进展[J].广东药学院学报,2014,30(6):784-787.
[11]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1996:518-520.
[12]孙赫,梁桂洪,林勇凯,等.高脂血症大鼠模型几种造模方法的筛选及优化[J].动物医学进展,2014,35(3):78-82.
[13]姬凤彩,姚刚,李琳琳,等.对大鼠高脂血症模型分型的探讨[J].新疆医科大学学报,2007,30(11):1297-1298.
[14]刘海利,肖学凤,莫凡,等.几种高脂血症实验动物模型的研究进展[J].实验动物科学,2010,27(2):38-41.
[15]张颖,孙根义,刘寅,等.冠状动脉粥样硬化与血脂代谢紊乱的因素分析[J].天津医药,2010,38(4):270-272.
[16]王燕.血液流变学指标与高脂血症的关系[J].检验医学与临床,2007,4(12):1179-1180.
[2]李彦斌,张志花,韩立民.冠心病(胸痹心痛)中医文献及病证研究[J].赣南医学院学报,2010,30(2):321-323.
[3]张振千.中医胸痹与冠心病心绞痛的相关性[J].航空航天医药,2010,21(9):1736-1737.
[4]陈宁.芹菜素对动脉粥样硬化大鼠血脂和血液流变学的影响[J].现代中西医结合杂志,2016,25(11):1155-1158.
[5]苏奋翔,张艳霞,安方玉,等.林氏降脂方对高脂血症大鼠血脂和血液流变学的影响[J].西部中医药,2015,28(10):12-14.
[6]胡锡琴,方欢乐,权志博,等.制何首乌对肝脏生化物质影响的实验研究[J].陕西中医学院学报,2007,30(6):63-64.
[7]Xiaoqing Wu,Xiaozhen Chen,Qingchun Huang,et al,Toxicity of raw and processed roots of polygonum multiflorum[J].Fitoterapia 2012,83(3):469-475.
[8]Jie Yu,Jie Xie,Xiao Jian,et al.Hepatoxicity of major constituents and extractions of radix polygoni multiflori and radix polygoni multiflori praeparata[J].Journal of Ethnopharmamcology 2011,137(3):1291-1299.
[9]Cardenas A,Restrepo J,Sierra F.Acute hepatitis due to shen-min:a herbal product de rived from polygonum multiflorum[J].JClinGastroenterol2006,40(7):629-632.
[10]闫二磊,梁生旺.何首乌肝损伤的研究进展[J].广东药学院学报,2014,30(6):784-787.
[11]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1996:518-520.
[12]孙赫,梁桂洪,林勇凯,等.高脂血症大鼠模型几种造模方法的筛选及优化[J].动物医学进展,2014,35(3):78-82.
[13]姬凤彩,姚刚,李琳琳,等.对大鼠高脂血症模型分型的探讨[J].新疆医科大学学报,2007,30(11):1297-1298.
[14]刘海利,肖学凤,莫凡,等.几种高脂血症实验动物模型的研究进展[J].实验动物科学,2010,27(2):38-41.
[15]张颖,孙根义,刘寅,等.冠状动脉粥样硬化与血脂代谢紊乱的因素分析[J].天津医药,2010,38(4):270-272.
[16]王燕.血液流变学指标与高脂血症的关系[J].检验医学与临床,2007,4(12):1179-1180.