利用分子标记技术对玉米自交系子粒油分的改良研究
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摘要
高油玉米是指子粒油分含量高于6%的玉米,是一种优良的粮油饲兼用型作物。高油玉米自交系By804含有qHO1和qHO6两个提高子粒油分的主效QTL。以By804为供体,利用分子辅助选择技术,结合田间农艺性状鉴定及核磁共振油分检测,将2个主效QTL分别回交转育至普通玉米自交系lx9801、lx03-2及lx00-1中。结果表明:来自By804的2个QTL均表现为随着回交次数的增加,子粒含油量较轮回亲本提高幅度逐步降低;不同遗传背景对QTL效应有较大影响:2个QTL在不同自交系的效应均以lx9801最佳,转育目标QTL后自交系绝对含油量较轮回亲本平均增加0.72%;2个位点效应相比,qHO6对子粒含油量的改良效果较优,3个自交系的改良系平均绝对含油量增加0.58%。
High-oil maize refers to maize with a grain oil content higher than 6%.It is an excellent crop of grain,oil and feed.The high oil maize inbred line By804 contains two major QTLs,qHO1 and qHO6.In this study,By804 was used as a donor,using linkage-labeled molecular-assisted selection technology,combined with field agronomic traits identification and nuclear magnetic resonance verification,two major QTLs were transferred to common maize inbred lines lx9801,lx03-2 and lx00-1 by backcrosses.The results showed that the growth trend of oil in different generations of improved inbred lines was similar.The generation BC1 F1 had the highest oil content increase,and with the increase of backcross algebra,the oil content of grain decreased gradually.Different genetic backgrounds had an effect on oil content.The average effect on lx9801 was the best with absolute oil content increases by 0.72% compared with the two-point effects.qHO6 was better in improving the grain oil content,which could increased the oil content of grain by an average of 0.58%.
High-oil maize refers to maize with a grain oil content higher than 6%.It is an excellent crop of grain,oil and feed.The high oil maize inbred line By804 contains two major QTLs,qHO1 and qHO6.In this study,By804 was used as a donor,using linkage-labeled molecular-assisted selection technology,combined with field agronomic traits identification and nuclear magnetic resonance verification,two major QTLs were transferred to common maize inbred lines lx9801,lx03-2 and lx00-1 by backcrosses.The results showed that the growth trend of oil in different generations of improved inbred lines was similar.The generation BC1 F1 had the highest oil content increase,and with the increase of backcross algebra,the oil content of grain decreased gradually.Different genetic backgrounds had an effect on oil content.The average effect on lx9801 was the best with absolute oil content increases by 0.72% compared with the two-point effects.qHO6 was better in improving the grain oil content,which could increased the oil content of grain by an average of 0.58%.
引文
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[15]Hong Y X,Qiu G Y,Bing Y J,et al.Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize.Theoretical&Applied Genetics,2010,120(3):665-678.
[2]范弘伟.高油玉米群体的杂种优势模式与高油授粉者选育.北京:中国农业大学,2001.
[3]Han Y,Parsons C M,Alexander D E.Nutritive value of high oil corn for poultry.Poultry Science,1987,66(1):103-111.
[4]Lambert R J,Hallauer A R.High-oil Corn Hybrids.London:CRCPress,1994.
[5]Lambert R J,Alexander D E,Mejaya I J.Single Kernel Selection for Increased Grain Oil in Maize Synthetics and High-Oil Hybrid Development.John Wiley&Sons,Inc.2004:153-175.
[6]Song T M,Chen S J.Long term selection for oil concentration in five maize populations[Zea mays L.;China].Maydica,2004,49(1):9-14.
[7]Clark D,Dudley J W,Rocheford T R,et al.Genetic analysis of corn kernel chemical composition in the random mated 10 generation of the cross of generations 70 of IHO×ILO.Translational Oncology,2006,3(6):373-379.
[8]Collard B C Y,Mackill D J.Marker-assisted selection:an approach for precision plant breeding in the twenty-first century.Philosophical Transactions:Biological Sciences,2008,363(1491):557-572.
[9]Xu Y B,Crouch J H.Marker-assisted selection in plant breeding:from publications to practice.Crop Science,2008,48(2):391-407.
[10]郝晓敏.利用两个高油主效QTL改良优良杂交种-郑单958的研究.北京:中国农业大学,2014.
[11]Hao X M,Li X W,Yang X H,et al.Transferring a major QTL for oil content using marker-assisted backcrossing into an elite hybrid to increase the oil content in maize.Molecular Breeding,2014,34(2):739-748.
[12]Guo Y,Yang X,Yang,et al.Identification of unconditional and conditional QTL for oil,protein and starch content in maize.The Crop Journal,2013,1(1):34-42.
[13]郭玉秋.高油玉米籽粒品质性状条件与非条件QTL分析及农艺性状QTL的定位.北京:中国农业大学,2011.
[14]李文才.粗山羊草D组染色体对小麦若干产量性状的影响.泰安:山东农业大学,2005.
[15]Hong Y X,Qiu G Y,Bing Y J,et al.Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize.Theoretical&Applied Genetics,2010,120(3):665-678.