Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers
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摘要
Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~+buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.
Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~+buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.
Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~+buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.
引文
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[3]Todd A K,Johnston M and Neidle S 2005 Nucleic Acids Res.33 2901
[4]Kaur P,Wu D,Lin J,Countryman P,Bradford K C,Erie D A,Riehn R,Opresko P L and Wang H 2016 Sci.Rep.6 20513
[5]Dai J,Carver M and Yang D 2008 Biochimie 90 1172
[6]Maizels N 2006 Nat.Struct.Mol.Biol.13 1055
[7]Cahoon L A and Seifert H S 2009 Science 325 764
[8]Rodriguez R,Miller K M,Forment J V,Bradshaw C R,Nikan M,Britton S,Oelschlaegel T,Xhemalce B,Balasubramanian S and Jackson S P 2012 Nat.Chem.Biol.8 301
[9]Siddiqui-Jain A,Grand C L,Bearss D J and Hurley L H 2002 Proc.Natl.Acad.Sci.USA 99 11593
[10]Wang H,Nora G J,Ghodke H and Opresko P L 2011 J.Biol.Chem.286 7479
[11]Dhakal S,Cui Y,Koirala D,Ghimire C,Kushwaha S,Yu Z,Yangyuoru P M and Mao H 2013 Nucleic Acids Res.41 3915
[12]Gray R D,Buscaglia R and Chaires J B 2012 J.Am.Chem.Soc.13416834
[13]Li W,Hou X-M M,Wang P-Y Y,Xi X G G and Li M 2013 J.Am.Chem.Soc.135 6423
[14]Laguerre A,Hukezalie K,Winckler P,Katranji F,Chanteloup G,Pirrotta M,Perrier-Cornet J M M,Wong J M and Monchaud D 2015 J.Am.Chem.Soc.137 8521
[15]Wang Y and Patel D J 1993 Structure 1 263
[16]Parkinson G N,Lee M P and Neidle S 2002 Nature 417 876
[17]Luu K N,Phan A T,Kuryavyi V,Lacroix L and Patel D J 2006 J.Am.Chem.Soc.128 9963
[18]You H,Zeng X,Xu Y,Lim C J,Efremov A K,Phan A T and Yan J2014 Nucleic Acids Res.42 8789
[19]Ju H P,Wang Y Z,You J,Hou X M,Xi X G,Dou S X,Li W and Wang P Y 2016 Acs.Omega.1 244
[20]Petraccone L,Spink C,Trent J O,Garbett N C,Mekmaysy C S,Giancola C and Chaires J B 2011 J.Am.Chem.Soc.133 20951
[21]Yu H,Gu X,Nakano S-i,Miyoshi D and Sugimoto N 2012 J.Am.Chem.Soc.134 20060
[22]Petraccone L,Trent J O and Chaires J B 2008 J.Am.Chem.Soc.13016530
[23]Li Y and Sugiyama H 2015 Chem.Commun.51 8861
[24]Abraham Punnoose J,Cui Y,Koirala D,Yangyuoru P M,Ghimire C,Shrestha P and Mao H 2014 J.Am.Chem.Soc.136 18062
[25]Lionnet T,Allemand J F F,Revyakin A,Strick T R,Saleh O A,Bensimon D and Croquette V 2012 Cold.Spring.Harb.Protoc.2012 34
[26]Chen H,Fu H,Zhu X,Cong P,Nakamura F and Yan J 2011 Biophys.J.100 517
[27]Zhao X,Zeng X,Lu C and Yan J 2017 Nanotechnology 28 414002
[28]Cnossen J P,Dulin D and Dekker N H 2014 Rev.Sci.Instrum.85103712
[29]Smith S B,Cui Y and Bustamante C 1996 Science 271 795
[30]Cluzel P,Lebrun A,Heller C,Lavery R,Viovy J L,Chatenay D and Caron F 1996 Science 271 792
[31]Fu H,Chen H,Marko J F and Yan J 2010 Nucleic Acids Res.38 5594
[32]Fu H,Chen H,Zhang X,Qu Y,Marko J F and Yan J 2011 Nucleic Acids Res.39 3473
[33]Zhang X,Chen H,Fu H,Doyle P S and Yan J 2012 Proc.Natl.Acad.Sci.USA 109 8103
[34]Zhang X,Chen H,Le S,Rouzina I,Doyle P S and Yan J 2013 Proc.Natl.Acad.Sci.USA 110 3865
[35]Xu Y,Ishizuka T,Kurabayashi K and Komiyama M 2009 Angew.Chem.Int.Ed.Engl.48 7833
[36]Ha¨nsel R,L o¨hr F,Trantirek L and D o¨tsch V 2013 J.Am.Chem.Soc.135 2816
[37]Cousins A R,Ritson D,Sharma P,Stevens M F,Moses J E and Searle M S 2014 Chem.Commun.50 15202
[38]Evans E and Ritchie K 1997 Biophys.J.72 1541
[39]Dhakal S,Cui Y,Koirala D,Ghimire C,Kushwaha S,Yu Z,Yangyuoru P M and Mao H 2013 Nucleic Acids Res.41 3915
[40]You H,Zeng X,Xu Y,Lim C J,Efremov A K,Phan A T and Yan J2014 Nucleic Acids Res.42 8789
[41]Yuan G,Le S,Yao M,Qian H,Zhou X,Yan J and Chen H 2017 Angew.Chem.Int.Ed.56 5490
[42]Cocco S,Yan J,Le′ger J-F F,Chatenay D and Marko J F 2004 Phys.Rev.E 70 11910
[43]Strick T R,Allemand J F,Bensimon D,Bensimon A and Croquette V1996 Science 271 1835
[44]Chen and H et al.2011 Biophys.J.100 517
[45]Lipfert J and Hao X and Dekker N H 2009 Biophys.J.96 5040
[46]Aggarwal T,Materassi D,Davison R,Hays T and Salapaka M 2012Cellular and Molecular Bioengineering 5 14