p28GANK蛋白表达与肝癌发病的相关性分析
|
摘要
目的分析p28GANK蛋白表达情况与原发性肝癌(PLC)发病的相关性。方法收集2015年3月—2018年3月河南省传染病医院肿瘤科行手术切除治疗并经病理证实的48例PLC患者的蜡块组织标本,其中有28例患者术中取得癌旁组织,均经10%甲醛溶液固定、石蜡包埋、切片,采用免疫组织化学S-P法染色法分析p28GANK蛋白表达水平,以同样方法评估组织标本中凋亡相关基因Bcl-2、Bax的蛋白质表达水平,并进行统计学分析。结果 p28GANK蛋白阳性表达多表现为棕黄色细小颗粒,定位于细胞核和细胞质。PLC组织中p28GANK蛋白及Bcl-2、Bax蛋白阳性表达率均明显高于癌旁组织(P<0.05)。Spearman等级相关分析显示,PLC组织中p28GANK蛋白与Bcl-2蛋白阳性表达存在明显相关性(P<0.05),而与Bax蛋白阳性表达不相关(P>0.05)。不同性别、年龄、最大肿瘤直径、肝功能分级的PLC患者组织中p28GANK蛋白阳性表达率无明显差异(P>0.05),但Ⅲ～Ⅳ期患者组织p28GANK蛋白阳性表达率明显高于Ⅰ～Ⅱ期患者,有淋巴结或远处转移的患者组织p28GANK蛋白阳性表达率明显高于无淋巴结或远处转移的患者(P<0.05)。结论 p28GANK蛋白表达与PLC的发生发展有关,高p28GANK蛋白表达可能通过上调凋亡抑制基因Bcl-2的表达而影响肿瘤侵袭与转移。
Objective To analyze the correlation between the expression of p28 GANK protein and the pathogenesis of primary liver cancer(PLC) and its possible role mechanism. Methods The paraffin embedded tissue specimens of 48 patients with PLC who underwent surgical resection and confirmed by pathology in Henan Provincial Infectious hospital from March 2015 to March 2018 were collected. Among them,28 patients were sampled adjacent tissues during surgery,and all of them were fixed with 10% formal dehyde solution,paraffin embedded and sliced. The p28 GANK protein expression level was analyzed by immunohistochemistry SP staining method. At the same method,the expression levels of apoptosis related-genes Bcl-2 and Bax proteins in tissue samples were evaluated and given statistical analysis. Results The positive expression of p28 GANK protein was mostly brown and yellow granules,located in the nucleus and cytoplasm. The positive expression rates of p28 GANK protein,Bcl-2 and Bax proteins in PLC tissues were significantly higher than those in adjacent tissues(P<0.05). Spearman level correlation analysis shows there was a significant correlation between the expression of p28 GANK protein and the positive expression of Bcl-2 protein in PLC tissues(P <0.05),but there was no correlation with the positive expression of Bax protein(P>0.05). There was no significant difference in the positive expression rate of p28 GANK protein in PLC patients with different gender,age,maximum tumor diameter and liver function grading(P>0.05),but the positive expression rate of p28 GANK protein in patients with stage Ⅲ-Ⅳ was significantly higher than that of patients with stage Ⅰ-Ⅱ,and the positive expression rate of p28 GANK protein in patients with lymph node or distant metastasis was obviously higher than that in patients without lymph node or distant metastasis(P <0.05). Conclusion The expression of p28 GANK protein is related to the occurrence and development of PLC:the high expression of p28 GANK protein may affect the invasion and metastasis of tumor by up-regulating the expression of apoptosis inhibitory gene Bcl-2.
Objective To analyze the correlation between the expression of p28 GANK protein and the pathogenesis of primary liver cancer(PLC) and its possible role mechanism. Methods The paraffin embedded tissue specimens of 48 patients with PLC who underwent surgical resection and confirmed by pathology in Henan Provincial Infectious hospital from March 2015 to March 2018 were collected. Among them,28 patients were sampled adjacent tissues during surgery,and all of them were fixed with 10% formal dehyde solution,paraffin embedded and sliced. The p28 GANK protein expression level was analyzed by immunohistochemistry SP staining method. At the same method,the expression levels of apoptosis related-genes Bcl-2 and Bax proteins in tissue samples were evaluated and given statistical analysis. Results The positive expression of p28 GANK protein was mostly brown and yellow granules,located in the nucleus and cytoplasm. The positive expression rates of p28 GANK protein,Bcl-2 and Bax proteins in PLC tissues were significantly higher than those in adjacent tissues(P<0.05). Spearman level correlation analysis shows there was a significant correlation between the expression of p28 GANK protein and the positive expression of Bcl-2 protein in PLC tissues(P <0.05),but there was no correlation with the positive expression of Bax protein(P>0.05). There was no significant difference in the positive expression rate of p28 GANK protein in PLC patients with different gender,age,maximum tumor diameter and liver function grading(P>0.05),but the positive expression rate of p28 GANK protein in patients with stage Ⅲ-Ⅳ was significantly higher than that of patients with stage Ⅰ-Ⅱ,and the positive expression rate of p28 GANK protein in patients with lymph node or distant metastasis was obviously higher than that in patients without lymph node or distant metastasis(P <0.05). Conclusion The expression of p28 GANK protein is related to the occurrence and development of PLC:the high expression of p28 GANK protein may affect the invasion and metastasis of tumor by up-regulating the expression of apoptosis inhibitory gene Bcl-2.
引文
[1]CHOI MS,KIM JH,DONG HS,et al.Mo1418-A role of scheduled second tace in early stage hepatocellular carcinoma showing complete response after the first tace[J].Gastroenterology,2017,152(5):S1177.
[2]HIGASHITSUJI H,ITOH K,NAGAO T,et al.Reduced stability of retinoblastoma protein by gankyrin,an oncogenic ankyrinrepeat protein overexpressed in hepatomas[J].Nature Medicine,2000,6(1):96.
[3]赵国宏,汤善宏,康建琴,等.p28^GANK的表达与食管鳞癌转移的相关性[J].细胞与分子免疫学杂志,2013,29(1):42-45.
[4]许洪宝,潘杰,汪伟民,等.p28GANK在食管胃交界部癌中的表达研究[J].浙江医学,2017,39(8):624-627.
[5]REN YB,LUO T,LI J,et al.p28(GANK)associates with p300to attenuate the acetylation of RelA[J].Molecular Carcinogenesis,2015,54(12):1626-1635.
[6]高杰,朱继业.中华人民共和国卫生部《原发性肝癌诊疗规范(2011年版)》解读[J].中华普通外科杂志,2012,27(8):693-695.
[7]陆录,钦伦秀.美国癌症联合委员会肝癌分期系统(第8版)更新解读[J].中国实用外科杂志,2017,37(2):141-145.
[8]蔡炜龙,许洪宝,汪伟民.p28GANK在结肠癌中的表达及其意义[J].胃肠病学,2013,18(2):105-108.
[9]王涛,温桂海,张桂东,等.N-乙酰半胱氨酸通过抑制HIF-1α下调肝癌HepG2细胞survivin和VEGF表达的实验研究[J].西部医学,2016,28(7):904-907.
[10]IWAI A,MARUSAWA H,KIUCHI T,et al.Role of a novel oncogenic protein,gankyrin,in hepatocyte proliferation[J].Journal of Gastroenterology,2003,38(8):751-758.
[11]PARKIN DM,BRAY F,FERLAY J,et al.Global cancer statistics,2002[J].Ca A Cancer Journal for Clinicians,2005,55(2):74.
[12]李世杰,杨奕,田玉龙,等.TACE后残余肝癌细胞β-catenin和p28 GANK的表达及意义的研究[J].实用肿瘤学杂志,2016,30(5):430-435.
[13]陈平平,李灼日.p28GANK在乙肝相关肝癌中表达意义[J].中国热带医学,2014,14(3):326-327.
[14]陈平平,刘初平,李灼日.p28GANK在肝门胆管癌表达的意义及其与预后的关系[J].中国普通外科杂志,2014,23(1):64-68.
[15]朱玉侠,赵明星,姜登鸽,等.抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达[J].胃肠病学和肝病学杂志,2016,25(9):1040-1043.
[16]莫瑞祥,杨威,李西融,等.p28GANK蛋白在肝细胞性肝癌组织中的表达及其临床意义[J].广西医学,2016,38(7):919-921.
[2]HIGASHITSUJI H,ITOH K,NAGAO T,et al.Reduced stability of retinoblastoma protein by gankyrin,an oncogenic ankyrinrepeat protein overexpressed in hepatomas[J].Nature Medicine,2000,6(1):96.
[3]赵国宏,汤善宏,康建琴,等.p28^GANK的表达与食管鳞癌转移的相关性[J].细胞与分子免疫学杂志,2013,29(1):42-45.
[4]许洪宝,潘杰,汪伟民,等.p28GANK在食管胃交界部癌中的表达研究[J].浙江医学,2017,39(8):624-627.
[5]REN YB,LUO T,LI J,et al.p28(GANK)associates with p300to attenuate the acetylation of RelA[J].Molecular Carcinogenesis,2015,54(12):1626-1635.
[6]高杰,朱继业.中华人民共和国卫生部《原发性肝癌诊疗规范(2011年版)》解读[J].中华普通外科杂志,2012,27(8):693-695.
[7]陆录,钦伦秀.美国癌症联合委员会肝癌分期系统(第8版)更新解读[J].中国实用外科杂志,2017,37(2):141-145.
[8]蔡炜龙,许洪宝,汪伟民.p28GANK在结肠癌中的表达及其意义[J].胃肠病学,2013,18(2):105-108.
[9]王涛,温桂海,张桂东,等.N-乙酰半胱氨酸通过抑制HIF-1α下调肝癌HepG2细胞survivin和VEGF表达的实验研究[J].西部医学,2016,28(7):904-907.
[10]IWAI A,MARUSAWA H,KIUCHI T,et al.Role of a novel oncogenic protein,gankyrin,in hepatocyte proliferation[J].Journal of Gastroenterology,2003,38(8):751-758.
[11]PARKIN DM,BRAY F,FERLAY J,et al.Global cancer statistics,2002[J].Ca A Cancer Journal for Clinicians,2005,55(2):74.
[12]李世杰,杨奕,田玉龙,等.TACE后残余肝癌细胞β-catenin和p28 GANK的表达及意义的研究[J].实用肿瘤学杂志,2016,30(5):430-435.
[13]陈平平,李灼日.p28GANK在乙肝相关肝癌中表达意义[J].中国热带医学,2014,14(3):326-327.
[14]陈平平,刘初平,李灼日.p28GANK在肝门胆管癌表达的意义及其与预后的关系[J].中国普通外科杂志,2014,23(1):64-68.
[15]朱玉侠,赵明星,姜登鸽,等.抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达[J].胃肠病学和肝病学杂志,2016,25(9):1040-1043.
[16]莫瑞祥,杨威,李西融,等.p28GANK蛋白在肝细胞性肝癌组织中的表达及其临床意义[J].广西医学,2016,38(7):919-921.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |