都柳江粗唇种群线粒体DNA Cyt b基因和D-loop序列组成及遗传多样性
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摘要
以采自都柳江40尾粗唇(Leiocassis crassilabris)个体为样品,采用PCR和DNA测序技术对粗唇都柳江种群Cyt b基因和D-loop序列进行了分析。获得了长度分别为1 118 bp、776~778 bp的粗唇Cyt b基因、Dloop序列。2段序列的碱基组成均为A+T的含量高于G+C的含量,但Cyt b基因碱基变异率(0.27%)显著低于D-loop的碱基变异率(3.34%~3.35%)。对粗唇D-loop序列结构进行了分析,识别了其终止序列区、中央保守区和保守序列区等的特征序列。都柳江粗唇40个个体Cyt b基因、D-loop序列中分别有3、20个多态位点,分别定义了4、10种单倍型。都柳江粗唇种群Cyt b基因和D-loop序列的单倍型多样性指数、核苷酸多样性指数和平均核苷酸差异数分别为0.146、0.000 13、0.150和0.404、0.003 17、2.444。都柳江粗唇种群遗传多样性较低,开展该种鱼类种群遗传多样性的研究和保护十分必要。
The sequences of Cyt b gene and D-loop in the species were analyzed using the methods of PCR and DNA sequencing according to the 40 individuals of L. crassilabris respectively from Dushan,Sandu,Rongjiang and Congjiang along the Duliu River. The sequences of 1 118 bp of the Cyt b gene and 776 ~ 778 bp of the D-loop were obtained respectively,and the quantity proportion of bases A + T in total bases was higher than that of bases G + C in both of the two sequences but the substitution rate of bases in the sequence of Cyt b gene( 0. 27%) was obviously less than that of D-loop( 3. 34% ~ 3. 35%) in the population. The characteristic sequences in the extended termination associated sequence,the central conserved domain and the conserved sequence block of D-loop were identified in L. crassilabris. 3 and 20 variable loci were detected as well as 4 and 10 haplotypes were defined respectively for the sequences of Cyt b gene and D-loop in the 40 individuals of L. crassilabris from the Duliu River. The haploidtype diversity,nucleotide diversity and average number of nucleotide differences were 0. 146,0. 000 13,0. 150 and 0. 404,0. 003 17,2. 444 respectively on the basis of the sequences of Cyt b gene and D-loop in the population. The population of L. crassilabris had low genetic diversity in the Duliu River now. Therefore,it's necessary to study and protect the genetic diversity of population of L. crassilabris from theDuliu River.
The sequences of Cyt b gene and D-loop in the species were analyzed using the methods of PCR and DNA sequencing according to the 40 individuals of L. crassilabris respectively from Dushan,Sandu,Rongjiang and Congjiang along the Duliu River. The sequences of 1 118 bp of the Cyt b gene and 776 ~ 778 bp of the D-loop were obtained respectively,and the quantity proportion of bases A + T in total bases was higher than that of bases G + C in both of the two sequences but the substitution rate of bases in the sequence of Cyt b gene( 0. 27%) was obviously less than that of D-loop( 3. 34% ~ 3. 35%) in the population. The characteristic sequences in the extended termination associated sequence,the central conserved domain and the conserved sequence block of D-loop were identified in L. crassilabris. 3 and 20 variable loci were detected as well as 4 and 10 haplotypes were defined respectively for the sequences of Cyt b gene and D-loop in the 40 individuals of L. crassilabris from the Duliu River. The haploidtype diversity,nucleotide diversity and average number of nucleotide differences were 0. 146,0. 000 13,0. 150 and 0. 404,0. 003 17,2. 444 respectively on the basis of the sequences of Cyt b gene and D-loop in the population. The population of L. crassilabris had low genetic diversity in the Duliu River now. Therefore,it's necessary to study and protect the genetic diversity of population of L. crassilabris from theDuliu River.
引文
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[18]刘焕章.鱼类线粒体控制区的结构和进化:以鳑鲏鱼类为例[J].自然科学进展,2002,12(3):266-270.
[19]魏刚,周丽,曾令江,等.大鰭鳠线粒体D-loop结构及其比较分析[J].西南大学学报(自然科学版),2015,37(1):46-52.
[20]赵亮,谢本贵,刘志瑾,等.太湖新银鱼线粒体D-loop和Cyt b片段序列结构与进化速率比较[J].动物学杂志,2010,45(2):27-38.
[21]董新培,穆淑梅,周楠,等.不同地理群体乌鳢线粒体DNA控制区结构分析及遗传多样性[J].水产学报,2014,38(9):1277-1285.
[22]荣朝振,祖国掌,胡建华,等.泥鳅线粒体DNA控制区结构分析及遗传多样性研究[J].南方水产科学,2011,7(5):55-62.
[23]姬南京,常亚青,赵冲,等.3个地理群体仿刺参D-loop序列的变异及系统发生分析[J].水产学报,2014,38(4):476-481.
[24]肖明松,崔峰,康健,等.淮河野生鲇鱼线粒体Cyt b基因的序列变异与遗传结构分析[J].动物学杂志,2013,48(1):75-86.
[25]李娜,陈少波,谢起浪,等.闽浙地区香鱼线粒体Cyt b基因和D-loop区序列多态性分析[J].遗传,2008,30(7):919-925.
[26]代应贵,韩雪,张晓杰.小口白甲鱼都柳江种群mt DNA D环的序列变异及遗传多样性[J].动物学杂志,2010,45(2):115-120.
[27]韩虎峰,代应贵.珠江水系光唇裂腹鱼可渡河种群mt DNA D-loop序列多态性分析[J].中国水产科学,2010,17(1):143-149.
[28]Grant W S,Bowen B W.Shallow population histories in deep evolutionary linages of marine fishes:Insights from sardines and anchovies and lessons for conservation[J].J Here,1998,89(5):415-426.
[29]郭新红,刘少军,刘巧,等.鱼类线粒体DNA研究新进展[J].遗传学报,2004,31(9):983-1000.
[30]陈灵芝.中国的生物多样性现状及其保护对策[M].北京:科学出版社,1993:99-113.
[2]Yang J Q,Tang W Q,Liao T Y,et al.Phylogeographical analysis on squalidus argentatus recapitulates historical landscapes and drainage evolution on the island of Taiwan and Mainland China[J].Internat J Mole Sci,2012,13(2):1405-1425.
[3]Yu M L,He S P.Phylogenetic relationships and estimation of divergence times among Sisoridae catfishes[J].Sci China Life Sci,2012,55(4):312-320.
[4]Yang Y,Kim K,Cothran E,et al.Genetic Diversity of Cheju Horses(Equuscaballus)Determined by using mitochondrial DNA D-loop Polymorphism[J].Biochem Genet,2002,40(5-6):175-186.
[5]陈永强,魏刚,李振华,等.粗唇精巢显微结构和精子发生的研究[J].淡水渔业,2008,38(1):6-10.
[6]李振华,陈永强,魏刚,等.嘉陵江粗唇卵巢组织学的周年变化[J].淡水渔业,2010,40(2):62-66.
[7]冯晓宇,郭水荣,谢楠,等.粗唇人工繁育的初步研究[J].水产科技情报,2008,35(4):157-163.
[8]彭作刚,何舜平,张耀光.细胞色素b基因序列变异与东亚鲿科鱼类系统发育[J].自然科学进展,2002,12(6):38-42.
[9]许宝青,刘凯,谢楠,等.钱塘江粗唇细胞色素b基因片段序列分析及系统发生研究[J].水产科学,2009,28(7):383-386.
[10]吕凤义,左艳玲,范月明,等.4种鲿科鱼类基于线粒体DNA D-loop序列的比较研究[J].华南师范大学学(自然科学版),2008,(2):105-110.
[11]张燕,张鹗,何舜平.中国鲿科鱼类线粒体DNA D-loop结构及其系统发育分析[J].水生生物学报,2003,27(5):463-467.
[12]Folmer O,Black M,Hoeh W,et al.DNA primer for amplification of mitochondrial cytochrome coxidase subunitⅠfrom diverse metazoan invertebrates[J].Mole Mar Biol Biotechnol,1994,3(5):294-299.
[13]钟立强,刘朋朋,潘建林,等.长江中下游5个湖泊黄颡鱼种群线粒体细胞色素b基因的遗传变异分析[J].湖泊科学,2013,25(2):302-308.
[14]刘朋朋,钟立强,潘建林,等.基于线粒体DNA D-loop分析黄颡鱼五个淡水湖泊群体的遗传多样性[J].海洋与湖沼,2013,44(3):728-733.
[15]彭作刚,张耀光,何舜平,等.从细胞色素b基因序列变异分析中国鲇形目鱼类的系统发育[J].遗传学报,2005,32(2):145-154.
[16]Loftus R T,Machugh D E,Bradley D G,et al.Evidence for two independent domestications of cattle[J].Proceed Nation Acad Sci United States of Am,1994,91(7):2575-2761.
[17]殷先兵,郝家胜,许丽,等.基于线粒体ND1和COI基因序列探讨锯眼蝶亚科主要类群的系统发生关系[J].动物学研究,2007,05:477-484.
[18]刘焕章.鱼类线粒体控制区的结构和进化:以鳑鲏鱼类为例[J].自然科学进展,2002,12(3):266-270.
[19]魏刚,周丽,曾令江,等.大鰭鳠线粒体D-loop结构及其比较分析[J].西南大学学报(自然科学版),2015,37(1):46-52.
[20]赵亮,谢本贵,刘志瑾,等.太湖新银鱼线粒体D-loop和Cyt b片段序列结构与进化速率比较[J].动物学杂志,2010,45(2):27-38.
[21]董新培,穆淑梅,周楠,等.不同地理群体乌鳢线粒体DNA控制区结构分析及遗传多样性[J].水产学报,2014,38(9):1277-1285.
[22]荣朝振,祖国掌,胡建华,等.泥鳅线粒体DNA控制区结构分析及遗传多样性研究[J].南方水产科学,2011,7(5):55-62.
[23]姬南京,常亚青,赵冲,等.3个地理群体仿刺参D-loop序列的变异及系统发生分析[J].水产学报,2014,38(4):476-481.
[24]肖明松,崔峰,康健,等.淮河野生鲇鱼线粒体Cyt b基因的序列变异与遗传结构分析[J].动物学杂志,2013,48(1):75-86.
[25]李娜,陈少波,谢起浪,等.闽浙地区香鱼线粒体Cyt b基因和D-loop区序列多态性分析[J].遗传,2008,30(7):919-925.
[26]代应贵,韩雪,张晓杰.小口白甲鱼都柳江种群mt DNA D环的序列变异及遗传多样性[J].动物学杂志,2010,45(2):115-120.
[27]韩虎峰,代应贵.珠江水系光唇裂腹鱼可渡河种群mt DNA D-loop序列多态性分析[J].中国水产科学,2010,17(1):143-149.
[28]Grant W S,Bowen B W.Shallow population histories in deep evolutionary linages of marine fishes:Insights from sardines and anchovies and lessons for conservation[J].J Here,1998,89(5):415-426.
[29]郭新红,刘少军,刘巧,等.鱼类线粒体DNA研究新进展[J].遗传学报,2004,31(9):983-1000.
[30]陈灵芝.中国的生物多样性现状及其保护对策[M].北京:科学出版社,1993:99-113.
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