推拿联合跑台训练对失神经大鼠不同时间神经吻合术后骨骼肌的影响
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摘要
目的:探讨推拿手法联合跑台训练对失神经大鼠在不同时间行吻合术后骨骼肌随干预时长增加的变化规律。方法:160只大鼠随机分为Ⅰ期缝合组(立即缝合)80只,Ⅱ期缝合组(4天缝合)80只。Ⅰ期缝合组分干预组40只,模型组40只;Ⅱ期缝合组分干预组40只,模型组40只。对干预组大鼠给予手法-跑台干预,模型组不做干预。结果:造模类型、干预手法、干预时长三个因素的主要效应均有显著意义,各因素主效应大小顺序为造模类型、干预时长、干预手法。结论:对于坐骨神经吻合术后大鼠的肌肉湿重比造模类型起主要作用,其次为干预时长,再次为干预手法。因此,失神经后及时予以神经吻合术,以及保证一定的干预时长,对于失神经支配肌肉术后的恢复至关重要。
Objective:To investigate the effect of manipulation of massage and running exercise on the change of skeletal muscle of rats' after the anastomosis.Method: A total of 160 rats were randomly divided into two groups. StageⅠ suture group(immediate suture after transection) composed of 80 rats. StageⅡsuture group(suture at fourth days after transection) also composed of 80 rats. Each group was divided into treatment group(40 cases)and control group(40 cases). Treatment groups accepted massage and running on treadmill every day. The control group was not treated.Result: The main effects of three factors were significant, and the order of main effects of each factor was modeling type, intervening time and intervening method.Conclusion: After sciatic nerve anastomosis in rats, the type of modeling played a major role in the wet weight ratio of muscle, followed by intervening time, and intervening manipulation again.Therefore, timely nerve anastomosis after denervation, as well as to ensure a certain intervening time, is essential for the recovery of denervated muscle after after nerve anastomosis.
Objective:To investigate the effect of manipulation of massage and running exercise on the change of skeletal muscle of rats' after the anastomosis.Method: A total of 160 rats were randomly divided into two groups. StageⅠ suture group(immediate suture after transection) composed of 80 rats. StageⅡsuture group(suture at fourth days after transection) also composed of 80 rats. Each group was divided into treatment group(40 cases)and control group(40 cases). Treatment groups accepted massage and running on treadmill every day. The control group was not treated.Result: The main effects of three factors were significant, and the order of main effects of each factor was modeling type, intervening time and intervening method.Conclusion: After sciatic nerve anastomosis in rats, the type of modeling played a major role in the wet weight ratio of muscle, followed by intervening time, and intervening manipulation again.Therefore, timely nerve anastomosis after denervation, as well as to ensure a certain intervening time, is essential for the recovery of denervated muscle after after nerve anastomosis.
引文
[1]朗斯塔夫(著),韩济生(主译).神经科学(中译本)[M].北京:科学出版社,2006:215-217
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[3]Wu P,Spinner RJ,Gu Y,et al.Delayed repair of the peripheral nerve:a novel model in the rat sciatic nerve[J].Journal of Neuroscience Methods,2013,214(1):37-44
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[6]Susan Y.Fu,Gordon T.Contributing factors to poor functional recovery after delayed nerve repair:prolonged axotomy[J].Journal of Neuroscience,1995,15(5):3876-3885.
[7]吴佳佳,徐建光,马书杰.失神经骨骼肌萎缩的机制及康复治疗[J].安徽医药,2017,21(11):1949-1953.
[8]邬江,孙晓娟,钟世镇,等.骨骼肌失神经和再神经化时肌卫星细胞的变化[J].中国修复重建外科杂志,2006(10):1047-1051.
[2]李应志,邰先桃,严隽陶.推拿在急性闭合性软组织损伤早期临床应用与研究现状[J].辽宁中医药大学学报,2014,16(11):80-82.
[3]Wu P,Spinner RJ,Gu Y,et al.Delayed repair of the peripheral nerve:a novel model in the rat sciatic nerve[J].Journal of Neuroscience Methods,2013,214(1):37-44
[4]Gordon T,Amirjani N,Edwards DC,et al.Brief postsurgical electrical stimulation accelerates axon regeneration and muscle reinnervation without affecting the functional measures in carpal tunnel syndrome patients[J].Experimental Neurology,2010,223(1):192-202.
[5]Lee SK,Wolfe SW.Peripheral nerve injury and repair[J].Journal of the American Academy of Orthopaedic Surgeons,2000,8(4):243-252.
[6]Susan Y.Fu,Gordon T.Contributing factors to poor functional recovery after delayed nerve repair:prolonged axotomy[J].Journal of Neuroscience,1995,15(5):3876-3885.
[7]吴佳佳,徐建光,马书杰.失神经骨骼肌萎缩的机制及康复治疗[J].安徽医药,2017,21(11):1949-1953.
[8]邬江,孙晓娟,钟世镇,等.骨骼肌失神经和再神经化时肌卫星细胞的变化[J].中国修复重建外科杂志,2006(10):1047-1051.