推拿联合跑台训练对大鼠坐骨神经吻合术后雪旺细胞及相关神经因子影响的研究
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摘要
目的:探讨推拿手法联合跑台训练对失神经大鼠立即行神经外模吻合术后神经再生的作用。方法:80只大鼠随机分为治疗组和模型组,各40只;对治疗组大鼠给予推拿手法-跑台训练干预1个月、2个月、3个月和4个月(1M、2M、3M和4M) 4个干预时长,模型组不做干预。结果:与模型组比较,治疗组大鼠的神经传导速度、神经生长因子(Nerve growth factor,NGF)均较高(P <0.01),而雪旺细胞无显著差异(P> 0.05)。与1M时比较,治疗组和模型组4M时大鼠的神经传导速度均较高;模型组4M时大鼠的NGF较高,治疗组3M、4M时大鼠的NGF均较高;治疗组和模型组大鼠的雪旺细胞在3M、4M时均较高,差异均有统计学意义(P <0.05)。结论:推拿联合跑台训练能够促进NGF的表达,进而促进坐骨神经吻合术后大鼠神经的再生。
Objective:To explore the effect of tuina combined with treadmill training on nerve regeneration in denervated rats which received epineurium anastomosis immediately. Methods:Divided 80 rats into the treatment group and the model group randomly,40 rats in each group. The treatment group was given the a total of four different intervention duration of tuina-treadmill training for one,two,three and four months(1M,2M,3M and 4M),while the model group was not given the intervention. Results: Compared with the model group,the nerve conduction velocity and NGF of rats in the treatment group were higher(P < 0.01),but there was no significant difference being found in Schwann cells(P > 0.05). Compared with 1M,the nerve conduction velocity of the rats at 4M in the treatment group and the model group were higher;the NGF of the rats at 4M in the model group was higher,and the NGF at 3M and 4M in the treatment group were higher; the Schwann cells at 3M and 4M in the treatmentgroup and the model group were higher, difference being significant(P < 0.05). Conclusion: The application of tuinacombined with treadmill training can promote the expression of NGF and then improve the regeneration of nerves in rats after sciatic nerve anastomosis.
Objective:To explore the effect of tuina combined with treadmill training on nerve regeneration in denervated rats which received epineurium anastomosis immediately. Methods:Divided 80 rats into the treatment group and the model group randomly,40 rats in each group. The treatment group was given the a total of four different intervention duration of tuina-treadmill training for one,two,three and four months(1M,2M,3M and 4M),while the model group was not given the intervention. Results: Compared with the model group,the nerve conduction velocity and NGF of rats in the treatment group were higher(P < 0.01),but there was no significant difference being found in Schwann cells(P > 0.05). Compared with 1M,the nerve conduction velocity of the rats at 4M in the treatment group and the model group were higher;the NGF of the rats at 4M in the model group was higher,and the NGF at 3M and 4M in the treatment group were higher; the Schwann cells at 3M and 4M in the treatmentgroup and the model group were higher, difference being significant(P < 0.05). Conclusion: The application of tuinacombined with treadmill training can promote the expression of NGF and then improve the regeneration of nerves in rats after sciatic nerve anastomosis.
引文
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[2]顾玉东.提高周围神经损伤的诊治水平[J].中华创伤骨科杂志,2003,5(1):1-4.
[3]周俊明,徐晓君,张沈煜,等.臂丛神经损伤规范化康复治疗的临床研究[J].中国康复医学杂志,2011,26(2):124-127,142.
[4]岳旭迎,李征宇,肖彬,等.推拿对全臂丛损伤术后患者的康复疗效观察[J].贵阳中医学院学报,2016,38(2):63-66.
[5]杨宇杰,薛景凤,刘豫安,等.结扎大鼠髂总动脉对坐骨神经传导速度的影响[J].中日友好医院学报,2001,15(1):30-32.
[6]刘玉丽,李野,任路,等.深刺“环跳”穴对大鼠损伤坐骨神经的修复作用[J].针刺研究,2014,39(2):93-99.
[7]Saika T,Senba E,Noguchi K,et al.Effects of nerve crush and transection on m RNA levels for nerve growth factor in the facial motoneurons[J].Brain Res Mol Brain Res,1991,9(1-2):157-160.
[8]Cui B,Wu C,Chen L,et al.One at a time,live tracking of NGF axonal transport using quantum dots[J].PNAS,2007,104(34):13666-13671.
[9]Peter E,Gabrel T,John Y,et al.Activated macrophages,and M icroglia induce dopaminergic sprouting in the injured striatum and express brain derived neurotrophic factoe and Glial cell Line Derived Neurotrophic Factor[J].The Journal of Neuroscience,1999,19(5):1708-1716.
[10]Lu L,Li W,Zhong C,et al.Increased apoptosis of immunoreactive host cells and augmented donor leukocyte chimerism,not sustained inhibition of B7 molecule expression are associated with prolonged cardiac allograft survival in mice preconditioned with immature donor dendritic cells[J].Transplantation,1999,68(6):747-757.
[11]蔡文琴.发育神经生物学[M].北京:科学出版社,2007.