地黄不同炮制品中无机元素的ICP-OES法测定
|
摘要
目的 探讨地黄不同炮制品中8种无机元素含量的变化。方法 采用微波消解样品,结合电感耦合等离子体发射光谱(ICP-OES)法对鲜地黄干燥品、生地黄和熟地黄饮片中主要常量元素Na,K,Ca,Mg和微量元素Fe,Cu,Mn,Zn等8种无机元素含量进行测定,采用SPSS 20.0对结果进行单因素方差分析。结果 在地黄不同炮制品中含量较高的元素为K,Ca,Mg,Na,其中K含量最高,在鲜地黄和生、熟地黄饮片中依次为11023.509,11098.181,9739.629 mg·kg~(-1);之后依次是Ca,Mg,Na测得含量较高;元素Fe,Zn,Mn,Cu含量较低,但其在不同炮制品中含量变化较大,其中Fe,Mn,Cu的含量顺序为:熟地黄饮片>生地黄饮片>鲜地黄干燥品,Zn的含量顺序为:鲜地黄干燥品>熟地黄饮片>生地黄饮片。结论 地黄不同炮制品中的无机元素含量变化较大,推测其为区别地黄不同炮制品功效作用的物质基础之一。
Objective To study the contents changes of 8 inorganic elements in different processed products of Rehmannia Radix.Methods The samples were treated by Microwave digestion,and the contents of 8 inorganic elements including Na, K, Ca, Mg and Fe, Cu, Mn, Zn in different processed products of Rehmannia Radix were determined by ICP-OES,the data were analyzed by one-way ANOVA with SPSS 20.0. Results The contents of inorganic elements K, Ca, Mg and Na were higher in different processed products of Rehmannia Radix, in which K content was the highest, which were 11023.509,11098.181,9739.629 mg·kg~(-1) in fresh Rehmannia Radix,dried Rehmannia Radix and Rehmannia Radix Preparata;then the contents of Ca,Mg,Na were higher;the contents of elements Fe, Zn, Mn and Cu were relatively lower, but their contents changed greatly in different processed products of Rehmannia Radix,and the order of Fe,Mn,Cu content in different processed products were:Rehmannia Radix Preparata>dried Rehmannia Radix>fresh Rehmannia Radix,the order of Zn content in different processed products was:fresh Rehmannia Radix>Rehmannia Radix Preparata>dried Rehmannia Radix.Conclusion The content of inorganic elements in different processed products of Rehmannia Radix varies greatly, which may be one of the material bases to distinguish their effects.
Objective To study the contents changes of 8 inorganic elements in different processed products of Rehmannia Radix.Methods The samples were treated by Microwave digestion,and the contents of 8 inorganic elements including Na, K, Ca, Mg and Fe, Cu, Mn, Zn in different processed products of Rehmannia Radix were determined by ICP-OES,the data were analyzed by one-way ANOVA with SPSS 20.0. Results The contents of inorganic elements K, Ca, Mg and Na were higher in different processed products of Rehmannia Radix, in which K content was the highest, which were 11023.509,11098.181,9739.629 mg·kg~(-1) in fresh Rehmannia Radix,dried Rehmannia Radix and Rehmannia Radix Preparata;then the contents of Ca,Mg,Na were higher;the contents of elements Fe, Zn, Mn and Cu were relatively lower, but their contents changed greatly in different processed products of Rehmannia Radix,and the order of Fe,Mn,Cu content in different processed products were:Rehmannia Radix Preparata>dried Rehmannia Radix>fresh Rehmannia Radix,the order of Zn content in different processed products was:fresh Rehmannia Radix>Rehmannia Radix Preparata>dried Rehmannia Radix.Conclusion The content of inorganic elements in different processed products of Rehmannia Radix varies greatly, which may be one of the material bases to distinguish their effects.
引文
[1] 国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2015:124.
[2] 奚香君,张永宁,袁丽超,等.香菇多糖、地黄煎影响小鼠免疫及内分泌功能的比较研究[J].辽宁中医药大学学报,2013,15(2):50.
[3] 沈华旦,方江济,郭鹏程,等.基于HPLC-UV-DPPH法的地黄和熟地黄药材抗氧化活性成分比较研究[J].中草药,2018,49(3):582.
[4] 简咏梅,孙永宁.六味地黄丸对N-甲基-N-亚硝基脲(MNU)诱导C57BL/KsJ-db/db小鼠胃癌的保护作用及其机制研究[J].中华中医药学刊,2015,33(5):1136.
[5] 贾绍华,张道勇,刘冰洁.地黄不同炮制品中水苏糖含量比较及其水苏糖抗肿瘤活性的研究[J].黑龙江医药,2012,25(4):511.
[6] 谢丽华,陈娟,李生强,等.六味地黄丸对绝经后骨质疏松症肾阴虚证JAK/STAT信号通路基因的影响[J].中国骨质疏松杂志,2014,20(7):741.
[7] 段卫娜,张振凌,孔莹莹,等.地黄不同炮制品组成的增液汤降低糖尿病大鼠血糖血脂作用的对比研究[J].中国实验方剂学杂志,2013,19(6):187.
[8] 王竹立,崔颖,叶美红.干地黄提取物A保护胃黏膜作用的形态学研究及与热休克蛋白的关系[J].中国中西医结合杂志,2005,25(Z1):38.
[9] 贾秀梅,张振凌,吴瑞环.鲜地黄及保鲜加工品对血热出血模型大鼠凉血止血药效比较[J].中国实验方剂学杂志,2014,20(6):127.
[10] 赵丹,张振凌,王胜超,等.不同方法炮制的熟地黄的补血作用比较[J].中国实验方剂学杂志,2017,23(19):46.
[11] 李更生,刘明,王慧森,等.地黄药材炮制过程中环烯醚萜苷类成分动态变化的研究[J].中国中医药科技,2008,15(6):440.
[12] 岳超,高杰,石上梅,等.HPLC测定地黄炮制前后3种苷类物质的含量[J].中国实验方剂学杂志,2015,21(4):71.
[13] 邱建国,张汝学,贾正平,等.生地黄不同炮制阶段寡糖和梓醇的变化研究[J].中草药,2011,42(12):2434.
[14] 卢鹏伟.地黄的化学成分和炮制的比较研究[D].河南大学药学部,2008:15.
[15] 李晓坤,张华锋,冯卫生,等.HPLC测定怀地黄中桃叶珊瑚苷的含量[J].中国现代应用药学,2013,30(2):171.
[16] 于文娜,张振凌,张颖,等.地黄炮制过程中异毛蕊花糖苷含量的动态变化[J].中国实验方剂学杂志,2017,23(18):22.
[17] 陶益,蒋妍慧,唐克建,等.地黄炮制前后化学成分的UHPLC-Q-TOF/MS比较研究[J].中药新药与临床药理,2016,27(1):102.
[18] 张文婷,岳超,黄琴伟,等.地黄生品与炮制品中8个糖类成分及不同炮制时间点其量变化分析[J].中草药,2016,47(7):1132.
[19] 高观祯,周建武,汪惠勤,等.地黄炮制过程氨基酸组分分析[J].氨基酸和生物资源,2010,32(03):52.
[20] 田柱萍,何邦平,王小燕,等.中药材的药效与其所含微量元素关系的研究进展[J].微量元素与健康研究,2005,22(4):54.
[21] 陈宇鸿.补血中药中微量元素的测定与分析[J].微量元素与健康研究,2018,35(2):39.
[22] 黄坚,倪静斌.中药微量元素锌,铜,铁,锰的研究[J].广东微量元素科学,1997,4(9):13.
[23] 丛涛,赵霖.微量元素锌铜铁硒锰的检测及临床营养学意义[J].微量元素与健康研究,2006,23(6):59.
[24] 黄作明,黄珣.微量元素与人体健康[J].微量元素与健康研究,2010,27(6):58.
[25] 徐军,傅喆暾.地黄炮制品名历史沿革及功效考辨探讨[J].中成药,2017,39(9):1913.
[2] 奚香君,张永宁,袁丽超,等.香菇多糖、地黄煎影响小鼠免疫及内分泌功能的比较研究[J].辽宁中医药大学学报,2013,15(2):50.
[3] 沈华旦,方江济,郭鹏程,等.基于HPLC-UV-DPPH法的地黄和熟地黄药材抗氧化活性成分比较研究[J].中草药,2018,49(3):582.
[4] 简咏梅,孙永宁.六味地黄丸对N-甲基-N-亚硝基脲(MNU)诱导C57BL/KsJ-db/db小鼠胃癌的保护作用及其机制研究[J].中华中医药学刊,2015,33(5):1136.
[5] 贾绍华,张道勇,刘冰洁.地黄不同炮制品中水苏糖含量比较及其水苏糖抗肿瘤活性的研究[J].黑龙江医药,2012,25(4):511.
[6] 谢丽华,陈娟,李生强,等.六味地黄丸对绝经后骨质疏松症肾阴虚证JAK/STAT信号通路基因的影响[J].中国骨质疏松杂志,2014,20(7):741.
[7] 段卫娜,张振凌,孔莹莹,等.地黄不同炮制品组成的增液汤降低糖尿病大鼠血糖血脂作用的对比研究[J].中国实验方剂学杂志,2013,19(6):187.
[8] 王竹立,崔颖,叶美红.干地黄提取物A保护胃黏膜作用的形态学研究及与热休克蛋白的关系[J].中国中西医结合杂志,2005,25(Z1):38.
[9] 贾秀梅,张振凌,吴瑞环.鲜地黄及保鲜加工品对血热出血模型大鼠凉血止血药效比较[J].中国实验方剂学杂志,2014,20(6):127.
[10] 赵丹,张振凌,王胜超,等.不同方法炮制的熟地黄的补血作用比较[J].中国实验方剂学杂志,2017,23(19):46.
[11] 李更生,刘明,王慧森,等.地黄药材炮制过程中环烯醚萜苷类成分动态变化的研究[J].中国中医药科技,2008,15(6):440.
[12] 岳超,高杰,石上梅,等.HPLC测定地黄炮制前后3种苷类物质的含量[J].中国实验方剂学杂志,2015,21(4):71.
[13] 邱建国,张汝学,贾正平,等.生地黄不同炮制阶段寡糖和梓醇的变化研究[J].中草药,2011,42(12):2434.
[14] 卢鹏伟.地黄的化学成分和炮制的比较研究[D].河南大学药学部,2008:15.
[15] 李晓坤,张华锋,冯卫生,等.HPLC测定怀地黄中桃叶珊瑚苷的含量[J].中国现代应用药学,2013,30(2):171.
[16] 于文娜,张振凌,张颖,等.地黄炮制过程中异毛蕊花糖苷含量的动态变化[J].中国实验方剂学杂志,2017,23(18):22.
[17] 陶益,蒋妍慧,唐克建,等.地黄炮制前后化学成分的UHPLC-Q-TOF/MS比较研究[J].中药新药与临床药理,2016,27(1):102.
[18] 张文婷,岳超,黄琴伟,等.地黄生品与炮制品中8个糖类成分及不同炮制时间点其量变化分析[J].中草药,2016,47(7):1132.
[19] 高观祯,周建武,汪惠勤,等.地黄炮制过程氨基酸组分分析[J].氨基酸和生物资源,2010,32(03):52.
[20] 田柱萍,何邦平,王小燕,等.中药材的药效与其所含微量元素关系的研究进展[J].微量元素与健康研究,2005,22(4):54.
[21] 陈宇鸿.补血中药中微量元素的测定与分析[J].微量元素与健康研究,2018,35(2):39.
[22] 黄坚,倪静斌.中药微量元素锌,铜,铁,锰的研究[J].广东微量元素科学,1997,4(9):13.
[23] 丛涛,赵霖.微量元素锌铜铁硒锰的检测及临床营养学意义[J].微量元素与健康研究,2006,23(6):59.
[24] 黄作明,黄珣.微量元素与人体健康[J].微量元素与健康研究,2010,27(6):58.
[25] 徐军,傅喆暾.地黄炮制品名历史沿革及功效考辨探讨[J].中成药,2017,39(9):1913.