不同生长调节剂对白花泡桐生理生化特性的影响
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摘要
以白花泡桐为试验材料,采用吲哚丁酸、萘乙酸、ABT生根粉配制不同浓度梯度试剂对泡桐进行浇灌,测定泡桐叶片中丙二醛、可溶性蛋白的含量以及超氧化物歧化酶、过氧化物酶的活性变化,了解不同生长调节剂对白花泡桐生长的生理作用.结果表明,与对照相比,吲哚丁酸处理下,叶片内丙二醛含量下降,可溶性蛋白含量增加、SOD活性和POD活性明显上升,其中,吲哚丁酸试剂在浓度为100 mg/kg时,比其他浓度处理效果更好.萘乙酸和ABT生根粉处理下,POD活性上升,丙二醛的含量低于对照.萘乙酸各浓度处理下SOD的活性和ABT生根粉处理下可溶性蛋白含量与对照相比较不理想.3种生长调节剂中,吲哚丁酸处理效果优于另外两种生长调节剂,且在100 mg/kg浓度时,促进效果最好.
Taking Paulownia fortunei as the research object and using IBA,NAA,ABT of rooting powder in different concentration gradient of reagent water activity in Paulownia,we measured the content of MDA and soluble protein in the leaves of Paulownia and superoxide dismutase,peroxidase,the physiological effects of different growth regulators on the growth of P. fortunei. The results showed that compared with the control,the content of malondialdehyde decreased,the content of soluble protein increased,SOD activity and POD activity increased obviously under the condition of indolbutyric acid treatment,and the indole butyric acid reagent had better effect than other concentrations when the concentration of 100 mg/kg. Under NAA and ABT rooting powder treatment,POD activity increased and MDA content was lower than the control. The concentration of SOD activity and ABT rooting powder treatment soluble protein content which compared with the control was not ideal. In the three growth regulators,the treatment effect of indolo butyric acid was better than the other two growth regulators,and the effect was the best at the concentration of 100 mg/kg.
Taking Paulownia fortunei as the research object and using IBA,NAA,ABT of rooting powder in different concentration gradient of reagent water activity in Paulownia,we measured the content of MDA and soluble protein in the leaves of Paulownia and superoxide dismutase,peroxidase,the physiological effects of different growth regulators on the growth of P. fortunei. The results showed that compared with the control,the content of malondialdehyde decreased,the content of soluble protein increased,SOD activity and POD activity increased obviously under the condition of indolbutyric acid treatment,and the indole butyric acid reagent had better effect than other concentrations when the concentration of 100 mg/kg. Under NAA and ABT rooting powder treatment,POD activity increased and MDA content was lower than the control. The concentration of SOD activity and ABT rooting powder treatment soluble protein content which compared with the control was not ideal. In the three growth regulators,the treatment effect of indolo butyric acid was better than the other two growth regulators,and the effect was the best at the concentration of 100 mg/kg.
引文
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[12]蒋彧,何俊蓉,袁刘丹,等.植物生长调节剂对彩色马蹄莲形态建成的影响及生理生化指标测定[J].西南农业学报,2010(1):184-187.
[13]张文会,郭磊,郭彦,等.植物生长激素对野生大豆耐盐性的影响[J].安徽农业科学,2007,35(5):1296-1297.
[14]弘娟,李沽维,蒋桥生,等.不同生长调节剂对中华猕猴桃扦插生根的影响[J].广西植物,2008,28(3):359-362.
[15]王慧,楼炉焕,朱小楼,等.不同植物生长调节物质对西南卫矛和卫矛扦插生根的影响[J].浙江林学院学报,2010,27(1):155-158.
[16]黎晓茜,龙友华,尹显慧,等.3种植物生长调节剂对烟草种子萌发和早期幼苗生长的影响[J].贵州农业科学,2016,44(10):22-26.
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[18]王清君,刘玉田,戴建国,等.不同种源及ABT1号生根粉对兴安落叶松造林的影响[J].林业科技,2000,25(4):4-5.
[19]裴海荣,李伟,张蕾,等.植物生长调节剂的研究与应用[J].山东农业科学,2015(7):142-146.
[20]刘军,曾德静,王铖,等.植物生长调节剂对海州常山硬枝扦插生根的影响[J].东北林业大学学报,2012,40(8):10-12.
[21]阎小芳,刘庆华,王奎玲,等.不同植物生长调节剂及浓度对曼地亚红豆杉扦插生根的影响[J].北方园艺,2014(20):56-62.
[22]程晓月,许宏刚,汉梅兰,等.3种植物生长调节剂对金叶接骨木扦插生根的影响[J].甘肃农业大学学报,2016,8(4):108-112.
[2]罗江华,李科,恩特马克·布拉提白.白花泡桐的研究进展[J].贵州农业科学,2010,38(4):200-Z02.
[3]李焕勇,刘涛,张华新,等.植物扦插生根机理研究进展[J].世界林业研究,2014,27(1):23-28.
[4]王英英.泡桐繁殖及造林技术[J].安徽农学通报,2016,22(3-4):90-91.
[5]杨立学,王海南,范晶,等.母株年龄和生长调节剂对俄罗斯大果沙棘嫩枝扦插生根的影响[J].北京林业大学学报,2011,33(6):107-111.
[6]满冰心,张龙,孙嘉志,等.植物生长调节剂促进红松提早结实技术研究[J].北华大学学报,2012,13(3):329-334.
[7]李红晓.ABT生根粉和保水剂对三种观赏树木移栽后生长和抗寒性的影响[D].保定:河北农业大学,2013.
[8]程国华,魏红,王建兴,等.泡桐育苗及栽培技术[J].中国园艺文摘,2014,10:140-141,222.
[9]矫鑫,刘世强,张保仁,等.NAA对甘草种子活力的影响[J].南阳师范学院学报,2013,12(12):37-41.
[10]贠玉洁,康永祥,赵宝鑫,等.毛梾硬枝扦插和嫁接繁殖技术研究[J].北方园艺,2011(21):60-64.
[11]王敏,姚维传,张从宇.植物生长调节剂对干旱胁迫下大豆幼苗生长的影响[J].水土保持学报,2005,19(4):190-192.
[12]蒋彧,何俊蓉,袁刘丹,等.植物生长调节剂对彩色马蹄莲形态建成的影响及生理生化指标测定[J].西南农业学报,2010(1):184-187.
[13]张文会,郭磊,郭彦,等.植物生长激素对野生大豆耐盐性的影响[J].安徽农业科学,2007,35(5):1296-1297.
[14]弘娟,李沽维,蒋桥生,等.不同生长调节剂对中华猕猴桃扦插生根的影响[J].广西植物,2008,28(3):359-362.
[15]王慧,楼炉焕,朱小楼,等.不同植物生长调节物质对西南卫矛和卫矛扦插生根的影响[J].浙江林学院学报,2010,27(1):155-158.
[16]黎晓茜,龙友华,尹显慧,等.3种植物生长调节剂对烟草种子萌发和早期幼苗生长的影响[J].贵州农业科学,2016,44(10):22-26.
[17]赵天宏,曹艳红,王岩,等.臭氧胁迫对大豆根系形态和活性氧代谢的影响[J].大豆科学,2012,31(1):52-57.
[18]王清君,刘玉田,戴建国,等.不同种源及ABT1号生根粉对兴安落叶松造林的影响[J].林业科技,2000,25(4):4-5.
[19]裴海荣,李伟,张蕾,等.植物生长调节剂的研究与应用[J].山东农业科学,2015(7):142-146.
[20]刘军,曾德静,王铖,等.植物生长调节剂对海州常山硬枝扦插生根的影响[J].东北林业大学学报,2012,40(8):10-12.
[21]阎小芳,刘庆华,王奎玲,等.不同植物生长调节剂及浓度对曼地亚红豆杉扦插生根的影响[J].北方园艺,2014(20):56-62.
[22]程晓月,许宏刚,汉梅兰,等.3种植物生长调节剂对金叶接骨木扦插生根的影响[J].甘肃农业大学学报,2016,8(4):108-112.