电项针干预对急性脑出血大鼠脑组织精氨酸加压素表达及神经功能缺损的影响
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摘要
目的研究电项针干预对急性脑出血大鼠脑组织精氨酸加压素(AVP)表达、脑组织含水量和神经功能缺损的影响,探讨电项针干预对脑出血急性期脑水肿和神经功能缺损的治疗作用。方法将84只的SD大鼠随机分为假手术组,模型组和电项针组;模型组和电项针组根据不同的干预时间分别设为1、3、5 d 3个亚组,对各组进行脑组织含水量、AVP含量检测及神经功能评分。结果模型组和电项针组各亚组AVP含量、脑组织含水量均高于假手术组(P <0.01),且电项针组较模型组降低(P <0.05)。模型组和电项针组AVP含量均为1 d开始升高,3 d达到峰值后回落,且电项针组始终低于模型组(P <0.05),脑组织含水量变化趋势与AVP含量变化一致。模型组和电项针组神经功能评分均高于假手术组,且电项针组评分较模型组低(P <0.05)。结论电项针能显著降低脑出血大鼠急性期AVP的表达及脑组织含水量,可以抑制脑出血急性期脑水肿的发展,并缓解脑出血后的神经功能缺损症状。
Objective: To investigate the effects of electro-nape acupuncture on AVP expression,water content of brain tissue and neurological deficit,and to explore the therapeutic effect of electro-nape acupuncture on brain edema and neurological deficit in acute cerebral hemorrhage rats. Methods: 84 SD rats were randomly divided into the sham operation group,the model group and electro-nape acupuncture group. The model group and the electro-nape acupuncture group were respectively set to 1 d,3 d and 5 d subgroups for different intervention time.Water content of brain tissue,AVP content and neurological function scores were detected for each group. Results: AVP content and water content of brain tissue in each subgroup of the model group and the electro-nape acupuncture group were higher than those of the sham operation group. And these indicators of electro-nape acupuncture group were lower than those of the model group(P < 0.01,P < 0.01,P < 0.05). AVP content of the model group and the electro-nape acupuncture group increased from 1 d,and fell after reaching the peak in 3 d.And the electro-nape acupuncture group was always lower than the model group(P < 0.05). The change trend of water content of brain tissue was consistent with the change of AVP content. The neurological function scores of the model group and the electro-nape acupuncture group were higher than those of the sham operation group. And the electro-nape acupuncture group was lower than the model group(P < 0.05). Conclusion: Electro-nape acupuncture can significantly reduce the expression of AVP,the water content of brain tissue in rats with acute time of cerebral hemorrhage,inhibit the development of brain edema and relieve the symptoms of neurological deficits after acute cerebral hemorrhage.
Objective: To investigate the effects of electro-nape acupuncture on AVP expression,water content of brain tissue and neurological deficit,and to explore the therapeutic effect of electro-nape acupuncture on brain edema and neurological deficit in acute cerebral hemorrhage rats. Methods: 84 SD rats were randomly divided into the sham operation group,the model group and electro-nape acupuncture group. The model group and the electro-nape acupuncture group were respectively set to 1 d,3 d and 5 d subgroups for different intervention time.Water content of brain tissue,AVP content and neurological function scores were detected for each group. Results: AVP content and water content of brain tissue in each subgroup of the model group and the electro-nape acupuncture group were higher than those of the sham operation group. And these indicators of electro-nape acupuncture group were lower than those of the model group(P < 0.01,P < 0.01,P < 0.05). AVP content of the model group and the electro-nape acupuncture group increased from 1 d,and fell after reaching the peak in 3 d.And the electro-nape acupuncture group was always lower than the model group(P < 0.05). The change trend of water content of brain tissue was consistent with the change of AVP content. The neurological function scores of the model group and the electro-nape acupuncture group were higher than those of the sham operation group. And the electro-nape acupuncture group was lower than the model group(P < 0.05). Conclusion: Electro-nape acupuncture can significantly reduce the expression of AVP,the water content of brain tissue in rats with acute time of cerebral hemorrhage,inhibit the development of brain edema and relieve the symptoms of neurological deficits after acute cerebral hemorrhage.
引文
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[11]Simard M,Nedergaard M.The neurobiology of glia in the context of water and ion homeostasis[J].Neuroscience,2004,129(4):877-878.
[12]王宏,姬丽娅,汪平,等.脑出血患者脑水肿与精氨酸加压素相关性研究[J].中华神经外科疾病研究杂志,2015,14(5):434-436.
[13]汪平,王宏,史航宇,等.脑出血患者精氨酸加压素与水代谢相关性研究[J].中华神经外科疾病研究杂志,2009,8(4):294-296.
[14]高维滨,刘勇,倪金霞,等.项针治疗中风后假性延髓麻痹的临床研究[J].上海针灸杂志,2009,28(1):19.
[15]朱冬梅.电针风池穴、供血穴治疗椎动脉型颈椎病的临床观察[J].针灸临床杂志,2009,25(7):41-42.
[16]Hertz L,Chern Y,Spater M.Involvement of non-neuronal brain cells in AVP-mediated regulation of water space at the cellular,organ and the whole-body level[J].J Neurosci Res,2000,62:480-490.
[2]王陇德.中国脑卒中防控要有战略与战术[J].医学研究杂志,2012,41(2):1.
[3]王宏,姬丽娅,汪平,等.脑出血患者脑水肿与精氨酸加压素相关性研究[J].中华神经外科疾病研究杂志,2015,14(5):434-436.
[4]王茜,唐美霞,崔海,等.电颈针干预对急性脑出血大鼠脑组织水通道蛋白4表达及脑含水量的影响[J].中国中医急症,2016,25(1):48-49.
[5]Del BM,Yan HJ,Buist R,et al.Experimental in tracerebral hemorrhage in rats.Magnetic resonance imaging and histopathological correlates[J].Stroke,1996,27(12):2312,2319.
[6]方宗畴.实验大鼠针灸俞穴图谱及针刺手法[J].南京铁道医学院学报,1993,12(1):19-21.
[7]华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991(1):1-5.
[8]Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84.
[9]苏伟.精氨酸加压素在创伤性脑水肿中的作用[D].杭州:浙江大学,2006.
[10]Ken,ichi Yamaguchi,Kazuo Watanabe.Anteroventral third ventricular N-methyl-d-aspartate receptors,but not metabotropic glutamate receptors are involved in hemorrhagic AVP secretion[J].Brain Research Bulletin,2005,66:59-61.
[11]Simard M,Nedergaard M.The neurobiology of glia in the context of water and ion homeostasis[J].Neuroscience,2004,129(4):877-878.
[12]王宏,姬丽娅,汪平,等.脑出血患者脑水肿与精氨酸加压素相关性研究[J].中华神经外科疾病研究杂志,2015,14(5):434-436.
[13]汪平,王宏,史航宇,等.脑出血患者精氨酸加压素与水代谢相关性研究[J].中华神经外科疾病研究杂志,2009,8(4):294-296.
[14]高维滨,刘勇,倪金霞,等.项针治疗中风后假性延髓麻痹的临床研究[J].上海针灸杂志,2009,28(1):19.
[15]朱冬梅.电针风池穴、供血穴治疗椎动脉型颈椎病的临床观察[J].针灸临床杂志,2009,25(7):41-42.
[16]Hertz L,Chern Y,Spater M.Involvement of non-neuronal brain cells in AVP-mediated regulation of water space at the cellular,organ and the whole-body level[J].J Neurosci Res,2000,62:480-490.