雌二醇降解菌Acinetobacter sp.DS1的筛选及降解条件优化
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摘要
筛选并鉴定出1株雌激素降解菌Acinetobacter sp.命名为DS1,对DS1降解17β-雌二醇实验条件进行优化,使用响应面法(RSM)对降解过程中底物浓度、接种量、培养温度和p H值进行了分析,构建响应面模型,并对反应条件进行优化。优化最佳降解条件为:17β-雌二醇量5.10 mg/L;接种量7.5%;p H值8.7;温度27℃。并进行试验验证,结果接近,响应面模型可有效描述降解过程,为降解工程应用和设计提供技术参考。
A strain of estrogen-degrading bacteria Acinetobacter sp.,named DS1,was screened from soil samples collected in proximity to a pharmaceutical factory in Beijing,and then was identified.To optimize the degradation of 17β-estradiol by DS1,a laboratory experiment was conducted by use of the response surface methodology(RSM) involving the parameters:substrate concentration,inoculation amount,culture temperature and pH value;those parameters were analyzed to construct a response surface model and the reaction conditions were optimized.Thereby,the optimum degradation condition was determined as follows:17β-estradiol was 5.10 mg/L,inoculation 7.5%,pH 8.7 and temperature 27 ℃;in addition,the optimized result was verified,which was indicating that RSM could well describe the degradation process,providing technical references for application and design of degradation engineering.
A strain of estrogen-degrading bacteria Acinetobacter sp.,named DS1,was screened from soil samples collected in proximity to a pharmaceutical factory in Beijing,and then was identified.To optimize the degradation of 17β-estradiol by DS1,a laboratory experiment was conducted by use of the response surface methodology(RSM) involving the parameters:substrate concentration,inoculation amount,culture temperature and pH value;those parameters were analyzed to construct a response surface model and the reaction conditions were optimized.Thereby,the optimum degradation condition was determined as follows:17β-estradiol was 5.10 mg/L,inoculation 7.5%,pH 8.7 and temperature 27 ℃;in addition,the optimized result was verified,which was indicating that RSM could well describe the degradation process,providing technical references for application and design of degradation engineering.
引文
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[19]刘鹏宇,夏传,常青,等.聚合硫酸铁混凝消除水中有机氯的研究[J].中国环境科学,2015(8):2382-2392.Liu Pengyu,Xia Chuan,Chang Qing,et al.Elimination of organic chlorine in water by coagulation of polymeric ferric sulfate[J].Chinese Journal of Environmental Science,2015(8):2382-2392.
[20]李会丽.大黄素纳米乳递释系统的优化及其药物动力学研究[D].兰州:兰州大学,2015.Li Huili.Optimization of Emodin Delivery System for Nanoemulsion and Its Pharmacokinetic Studies[D].Lanzhou:Lanzhou University,2015.
[2]陈明,杨小丽,沈丹群,等.长江中下游某Unitank污水处理厂去除雌激素的效果分析[J].环境工程,2012,30(2):1-5.Chen Ming,Yang Xiaoli,Shen Danqun,et al.Effect of estrogenic removal in a Unitank wastewater treatment plant in the middle and lower reaches of the Yangtze River[J].Environmental Engineering,2012,30(2):1-5.
[3]李红萍,孙志武,李好,等.污水处理工艺中雌激素活性的分布与去除研究[J].环境科学与技术,2016,39(10):128-132.Li Hongping,Sun Zhiwu,Li Hao,et al.Distribution and removal of estrogenic activity in wastewater treatment process[J].Environmental Science and Technology,2016,39(10):128-132.
[4]纪付元,田云飞,袁守军,等.甾体雌激素在不同污水处理工艺中的去除规律[J].中南大学学报:自然科学版,2017,48(4):873-879.Ji Fuyuan,Tian Yunfei,Yuan Shoujun,et al.Elimination of steroidal estrogen in different wastewater treatment processes[J].Journal of Central South University:Science and Technology,2017,48(4):873-879.
[5]Fujii K,Kikuchi S,Satomi M,et al.Degradation of 17β-estradiol by a gram-negative bacterium isolated from activated sludge in a sewage treatment plant in Tokyo,Japan[J].Applied and Environmental Microbiology,2002,68(4):2057-2060.
[6]Yoshimoto T,Nagai F,Fujimoto J,et al.Degradation of estrogens by Rhodococcus zopfii and Rhodococcus equi isolates from activated sludge in wastewater treatment plants[J].Applied and Environmental Microbiology,2004,70(9):5283-5289.
[7]Zeng Q,Li Y,Gu G,et al.Sorption and biodegradation of 17β-estradiol by acclimated aerobic activated sludge and isolation of the bacterial strain[J].Environmental Engineering Science,2009,26(4):783-790.
[8]杨俊.17β-雌二醇的生物降解模式及高效降解菌的降解特性研究[D].杭州:浙江工业大学,2010.Yang Jun.Biodegradation Mode of 17β-estradiol and Degradation Characteristics of Highly Efficient Degrading Bacteria[D].Hangzhou:Zhejiang University of Technology,2010.
[9]杨俊,姜理英,陈建孟.1株17β-雌二醇高效降解菌的分离鉴定及降解特性[J].环境科学,2010(5):1313-1319.Yang Jun,Jiang Liying,Chen Jianmeng.Isolation,identification and degradation characteristics of a 17β-estradiol highly efficient degradation bacteria[J].Environmental Science,2010(5):1313-1319.
[10]李欣,凌婉婷,刘静娴,等.固定化菌剂对污水和牛粪中雌二醇和己烯雌酚的去除作用[J].环境科学,2015(7):2581-2590.Li Xin,Ling Wanting,Liu Jingxian,et al.Effects of immobilized microbial agents on the removal of estradiol and diethylstilbestrol in sewage and cattle manure[J].Environmental Science,2015(7):2581-2590.
[11]Lee H,Liu D.Degradation of 17β-estradiol and its metabolites by sewage bacteria[J].Water,Air&Soil Pollution,2002,134(1):351-366.
[12]Li Z,Nandakumar R,Madayiputhiya N,et al.Proteomic analysis of 17β-estradiol degradation by Stenotrophomonas maltophilia[J].Environmental Science and Technology,2012,46(11):5947-5955.
[13]Li F,Yuasa A,Obara A,et al.Aerobic batch degradation of17-βestradiol(E2)by activated sludge:effects of spiking E2 concentrations,MLVSS and temperatures[J].Water Research,2005,39(10):2065-2075.
[14]周海东,黄霞,王晓琳,等.北京市城市污水雌激素活性的研究环境科学,2009,30(12):3590-3595.
[15]梁超.鹅肉超高压保鲜的研究[D].长春:吉林农业大学,2011.Liang Chao.Study on Ultra High Pressure Preservation of Goose Meat[D].Changchun:Jilin Agricultural University,2011.
[16]周力,陈哲,胡传荣,等.响应面法优化山茱萸籽蛋白制备工艺条件的研究[J].食品工业,2016(9):58-62.Zhou Li,Chen Zhe,Hu Chuanrong,et al.Optimization of processing conditions for hawthorn seed proteins by response surface methodology[J].Food Industry,2016(9):58-62.
[17]贺湘,赵振刚,于淑娟,等.葡聚糖酶应用于甘蔗混合汁的工艺优化[J].食品工业科技,2012(4):330-335.He Xiang,Zhao Zhengang,Yu Shujuan,et al.Application of dextranase in sugarcane juice mixing process optimization[J].Food Science,2012(4):330-335.
[18]林泳墨.环境雌激素中17β-雌二醇降解菌的筛选及其降解特性研究[D].长春:吉林农业大学,2016.Lin Yongmo.Screening and Degradation Characteristics of17β-estradiol Degrading Bacteria in Environmental Estrogens[D].Changchun:Jilin Agricultural University,2016.
[19]刘鹏宇,夏传,常青,等.聚合硫酸铁混凝消除水中有机氯的研究[J].中国环境科学,2015(8):2382-2392.Liu Pengyu,Xia Chuan,Chang Qing,et al.Elimination of organic chlorine in water by coagulation of polymeric ferric sulfate[J].Chinese Journal of Environmental Science,2015(8):2382-2392.
[20]李会丽.大黄素纳米乳递释系统的优化及其药物动力学研究[D].兰州:兰州大学,2015.Li Huili.Optimization of Emodin Delivery System for Nanoemulsion and Its Pharmacokinetic Studies[D].Lanzhou:Lanzhou University,2015.